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1

Why use the mini-FLOTAC to detect metastrongyloid larvae in dogs and cats?

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Ianniello D., Pepe P., Alves l. C., Ciuca L., Maurelli M. P., Amadesi A., Bosco A., Musella V., Cringoli G., Rinaldi L.
Acta Parasitologica
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2020
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tom 65
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nr 2
2

An additional asset for the FLOTAC technique: detection of gastrointestinal parasites in vegetables

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Nascimento Ramos I. C., Nascimento Ramos R. A., Giannelli A., Santana Lima V. F., Cringoli G., Rinaldi L., de Carvalho G. A., Alves L. C.
Acta Parasitologica
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2019
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tom 64
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nr 2
3

The comparison of FLOTAC, FECPAK and McMaster techniques for nematode egg counts in cattle

100%
Bosco A., Rinaldi L., Maurelli M. P., Musella V., Coles G. C., Cringoli G.
Acta Parasitologica
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2014
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tom 59
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nr 4
Three methods, FLOTAC, FECPAK and McMaster were compared for accuracy and sensitivity for counting numbers of nematode eggs in faeces of naturally infected cattle with high or low nematode egg counts. Only FLOTAC gave positive results for 12 replicates from pooled samples with low egg counts making it more sensitive than FECPAK (67%) and McMaster (41.7%). FLOTAC resulted in generally higher egg counts and lower coefficients of variation than the other two methods used. The reliability of FECPAK and McMaster is depended on the area under the slide counted. All three methods can be used for making decisions whether to treat but FLOTAC or Mini-FLOTAC should be used for faecal egg count reduction tests when lower egg counts are present.
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A comparison of FLOTAC and CFF techniques in detecting gastrointestinal parasites in water buffaloes (Bubalus bubalis)

84%
Salvador R. T., Abalos R. P., Ruba A. M., Mingala C. N.
Annals of Parasitology
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2014
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tom 60
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nr 2
The objective of the study was to compare the usefulness of FLOTAC and centrifugal fecal flotation (CFF) techniques. More specifically, the taxonomic classes (Nematoda and Cestoda) of endoparasites present in fecal samples of buffaloes are identified, the sensitivity and specificity of FLOTAC relative to CFF are calculated, and the agreement of both techniques is evaluated using Kappa statistics. Fresh fecal samples from 220 buffaloes in 10 municipalities were collected. Sheather’s sugar was used as a flotation solution for both the FLOTAC and CFF techniques. Of the 220 animals, 109 samples were nematode positive and 111 samples were nematode negative according to the FLOTAC technique, while 74 were found to be positive and 146 negative according to the CFF technique. No cestodes were detected by either technique. The calculated sensitivity for FLOTAC is 89.19% and its specificity is 70.55%. Kappa statistics revealed moderate agreement (k=0.535) between the two techniques in detecting nematodes. The prevalence observed based on FLOTAC and CFF test were 49.54% (109/220; 95% CI: 47.75–56.34) and 33.64% (72/220; 95% CI: 27.42–40.3), respectively.
5

Parasitic infections detected by FLOTAC in zoo mammals from Warsaw, Poland

67%
Maesano G., Capasso M., Ianniello D., Cringoli G., Rinaldi L.
Acta Parasitologica
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2014
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tom 59
|
nr 2
The aim of this study was to estimate the occurrence of intestinal parasites in groups of mammals kept in the Warsaw zoological garden (Poland). 71 pools of fecal samples were analyzed using the FLOTAC techniques. 48% of animals were positive and 47% of positivities showed multiple infections. Toxocara cati (71.4%) was found in felines; marsupials were infected with Coccidia (90%). Giardia spp. (24.0%), Blastocystis spp. (12.3%), Iodamoeba spp. (10.0%), Enterobius vermicularis (6.0%) and Entamoeba coli (3.3%) were found in primates. Gastrointestinal strongyles (60.5%) were prevalent in ruminants which resulted positive also to Coccidia (Eimeria spp. = 50.0%), Trichuris spp. (25.0%) and Nematodirus (14.0%). Strongyles (34.0%) were the most frequent parasites in monogastric herbivores, followed by Parascaris equorum (17.0%). None of the animals showed any symptom associated with gastrointestinal parasitic infections. According to our results the need to prevent, diagnose, control, and treat intestinal parasitism trough specific control programs is mandatory for animal welfare in order to limit the spread of parasitic infections in animals and humans.
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