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Ultrastructure of the spermatozoon of Paroniella reynoldsae [Cyclophyllidea, Davaineidae] an intestinal parasite of Corvus albus [Aves, Corvidae]

100%

Ba C. T., Ba A., Marchand B.

Acta Parasitologica

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2005

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tom 50

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nr 3

The mature Paroniella reynoldsae spermatozoon exhibits an apical cone of electron-dense material about 2.2 µm long and 0.65 µm wide at its base and two helicoidal crest-like bodies roughly 100 to 150 nra thick. The latter are of different lengths, spiralled and make an angle of about 45° with the spermatozoon axis. The axoneme is of the 9 + '1' trepaxonematan pattern and does not reach the posterior extremity of the gamete. The nucleus is an electron-dense cord 0.25 µm thick coiled in a spiral around the axoneme. The cytoplasm exhibits a posterior densification and contains few small electron-dense granules in regions I, II and V of the spermatozoon. In regions III and IV, it is divided into irregular compartments by walls of electron-dense material. The cortical microtubules are spiralled at an angle of about 45°. The presence of an electron-lucent apical cone containing numerous small granules of electron-dense material has never, to our knowledge, been reported in a cestode. Likewise, a crest-like body forming a terminal spot of electron-dense material located in the prolongation of the apical cone has never been described before in a cestode. Moreover, in this study, we try to show the existence of tight reciprocal phylogenetic relationships between genera within the Davaineidae and the Anoplocephalidae.

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In search of mitochondrial markers for resolving the phylogeny of cyclophyllidean tapeworms [Platyhelminthes, Cestoda] - a test study with Davaineidae

100%

Littlewood D. T. J., Waeschenbach A., Nikolov P. N.

Acta Parasitologica

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2008

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tom 53

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nr 2

The most species rich order of tapeworms is the Cyclophyllidea and prior to wide-scale sampling of these worms for phylogenetics, we wished to develop reliable PCR primers that would capture fragments of mitochondrial (mt) DNA with phylogenetic utility across the order. Nuclear ribosomal RNA gene sequences are well-established and valuable markers for resolving flatworm interrelationships spanning a wide range of taxonomic divergences, but fail to provide resolution amongst recently diverged lineages. Entire mt genomes of selected cyclophyllidean tapeworms are available on GenBank, and we used these to design PCR primers to amplify mtDNA from cox1, rrnL and nad1 for a range of cyclophyllideans (7 davaineids, 1 hymenolepidid and 1 dilepidid) and selected outgroups (Tetrabothrius sp. and Mesocestoides sp.). A combined nuclear and mt gene data set was used to estimate a reference phylogeny and the performance of the individual genes was compared to this. Although nuclear and mt genes each contributed to the structure and stability of the phylogenetic estimate, strongest nodal support was provided by nuclear data amongst the basal lineages and by mt data amongst the most recently diverged lineages. The apparent complementarity afforded by combining nuclear and mt data was compromised by these data partitions providing conflicting signal at poorly supported nodes. Nevertheless, we argue for a combined evidence approach. PCR primers that amplify rrnL were designed and tested successfully against a diversity of cyclophyllideans; rrnL and nad1 appeared to be more informative than the fragment of cox1. The genus Raillietina was not supported by molecular evidence. The new primers will likely provide considerable resolution to estimates of cyclophyllidean interrelationships in future studies.

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Skrjabini - cestodiasis of poultry chickens of the south of Ukraine

80%

Stepanova N. O., Bogach M. V., Greben O. B., Kornyushyn V. V.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

Ograniczanie wyników

2 Acta Parasitologica

1 Annals of Parasitology

1 Ba A.

1 Ba C. T.

1 Bogach M. V.

1 Greben O. B.

1 Kornyushyn V. V.

1 Littlewood D. T. J.

1 Marchand B.

1 Nikolov P. N.

1 Stepanova N. O.

1 Waeschenbach A.

1 2016

1 2008

1 2005

Ultrastructure of the spermatozoon of Paroniella reynoldsae [Cyclophyllidea, Davaineidae] an intestinal parasite of Corvus albus [Aves, Corvidae]

In search of mitochondrial markers for resolving the phylogeny of cyclophyllidean tapeworms [Platyhelminthes, Cestoda] - a test study with Davaineidae

Skrjabini - cestodiasis of poultry chickens of the south of Ukraine