Wyniki wyszukiwania

1

Species identification of Diplostomum pseudospathaceum Niewiadomska, 1984 and D.paracaudum [Iles, 1959] metacercariae using DNA polymorphism amplified by arbitrary primers

100%

Laskowski Z.

Acta Parasitologica

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1996

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tom 41

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nr 1

Metacercariae of Diplostomum pseudospathaceum and D. paracaudum were examined for genetic differences by random amplified polymorphic DNA analysis. Eleven different oligonucleotide decamers with arbitrary DNA sequence were tested as primers to amplify DNA from D. pseudospathaceum and D. paracaudum. Depending on the primer, the number of reproducible amplified fragments varied from 7 to 15, ranging in size from 0.2 to 2.0 kb DNA. Reproducible differences in patterns allowed differentiation of the two species with each primer. RAPD patterns unambiguously discriminated between D. pseudospathaceum and D. paracaudum, allowing good species identification.

2

DNA polymorphism of introns 1 and 2 of prolactin receptor gene and its association with litter size in goats

88%

Ran D., Jing Y., Ming-Xing C., Gui-Ling C., Tao F., Li F., Zhong-Xiao Z.

Animal Science Papers and Reports

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2011

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tom 29

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nr 4

The prolactin receptor (PRLR) gene was studied as a candidate gene for the high prolificacy of Jining Grey goats. Polymorphisms in intron 1 and intron 2 of PRLR gene were detected in high prolificacy (Jining Grey) and low prolificacy (Boer, Wendeng dairy, Liaoning Cashmere and Beijing) native goats using PCR-SSCP. For intron 1, five genotypes (AA, AH, AK, HH and HK) were identified in Jining Grey goats, and two (AA and AK) in the other four breeds. The Jining Grey does of genotype HH, HK, AH and AK delivered by 0.65, 0.62, 0.59 and 0.57 more kids (P<0.01) than those of genotype AA, respectively. For intron 2, three genotypes (CC, CD and DD) were detected in Boer goats, and two (CC and CD) in the other four breeds. The Jining Grey does of genotype CD delivered by 0.55 (P<0.01) more kids than those of genotype CC.

3

Silent point mutation polymorphism of the bovine CD18 encoding gene

88%

Czarnik U., Zabolewicz T., Galinski M., Pareek C. S., Walawski K.

Journal of Applied Genetics

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2004

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tom 45

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nr 1

4

DNA polymorphism of the alphaA-globin gene in domestic pigeon

75%

Dybus A., Chang M.-H., Cheng Y.-H., Szatkowska I.

Animal Science Papers and Reports

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2008

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tom 26

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nr 3

The study aimed at identifying the polymorphism of domestic pigeon αA-globin gene which can be a potential homing marker in selection of racing pigeons. A total of 329 domestic pigeons were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP)method. The PCR products were digested with 17 restriction endonucleases. One RFLP – detected with HphI – was found in intron 1 and represented a C/T mutation. The second RFLP – detected with BseLI – was a point mutation in the 3’UTR region of the gene (C/T mutation). The polymorphism in the 3’UTR region of the pigeon αA-globin gene can potentially affect the stability of mRNA and modify the gene expression. The mechanisms of haemoglobin function reflecting variants of the αA-globin gene remain unknown.

5

Searching for the most useful genotypes of Lupinus mutabilis sweet for breeding purpose

63%

Galek R., Kozak B., Sawicka-Sienkiewicz E., Zalewski D., Nowosad K.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2017

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tom 20

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nr 4

6

PCo-A analysis of strawberry germplasm used in European breeding programs, based on evaluation of DNA polymorphism of investigated plants

63%

Kuras A., Korbin M.

Journal of Fruit and Ornamental Plant Research

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2010

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tom 18

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nr 2

7-16

The level of genetic relatedness of ninety-six strawberry cultivars, released in different breeding centres of seventeen countries, was estimated based on analysis of their DNA polymorphism. Five hundred fifty-eight polymorphic amplicons, with a size range from 80 to 2600 bp, were generated in PCRs carried out on the template of DNA isolated from plants representing all analyzed cultivars. In RAPD reactions, polymorphic bands covered 58% of the total number of PCR products, while in ISSR, SSR and selective AFLP, the polymorphic DNA fragments covered 75%, 70% and 67% of all amplicons, respectively. Data concerning DNA polymorphism were assembled using the PCo-A method (Principal Component Analysis), and then referred to information about country of origin and pedigree described by the breeders. The results showed that contemporary breeding uses genetic resources in a very narrow range. Consequently, the cultivars released in individual breeding centres presented a very close relationship and were grouped in one, or at most two, genetic clusters.

7

The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil

63%

Werner M., Irzykowska L.

Phytopathologia Polonica

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2007

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tom 46

8

Dopamine D2 receptor gene polymorphism and body mass

63%

Wlodarczyk M., Jarosz A., Nowicka G.

Żywienie Człowieka i Metabolizm

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2009

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tom 36

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nr 4

Dopamine plays a role in the regulation of appetite. Polymorphism in dopamine receptor gene may influence obesity development by their potential impact on dopamine reward function. The aim of the study was to investigate prevalence of TaqI A D2 dopamine receptor genotypes in relation to degree of obesity. Polymorphic variants of DRD2 gene was determined by PCR-RFLP method in 266 subjects. The A1 allele and the A1 allele containing genotypes were more frequent in the obese subjects than in non-obese subjects (43% vs. 14% for A1A1; 40% vs. 35% for A1A2; 32% vs 22% for A1 allele, respectively). Our findings are in line with the hypothesis of the involvement of gene implicated in dopamine regulation in development of obesity.

9

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Badanie polimorfizmu rzepakochwastow za pomoca markerow RAPD

63%

Aleksandrzak L., Broda Z.

Rośliny Oleiste - Oilseed Crops

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2004

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tom 25

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nr 1

61-66

10

Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers

63%

Okon S., Paczos-Grzeda E., Loboda M., Sugier D.

Acta Scientiarum Polonorum. Hortorum Cultus

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2014

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tom 13

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nr 4

Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.

11

Analysis of DNA polymorphism [RAPD-PCR] and reciprocal effects of geese crossbreeds

63%

Lisowski M., Slawinska A., Dluzniewska P., Mazanowski A., Bednarczyk M.

Folia Biologica

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2008

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tom 56

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nr 3-4

159-164

12

Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo

63%

Wachowiak W., Baczkiewicz A., Celinski K., Prus-Glowacki W.

Dendrobiology

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2004

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tom 51

67-72

Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.

13

Identification of pear cultivars with RAPD and ISSR markers

63%

Lisek A., Rozpara E.

Journal of Fruit and Ornamental Plant Research

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2010

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tom 18

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nr 2

17-22

RAPD and ISSR techniques were used in identification of 26 pear cultivars. As a result of reactions carried out with RAPD 25 primers, 103 polymorphic DNA fragments were obtained. The largest number of polymorphic dNa fragments (7-8) was produced in reactions with the following primers: OPT 15, OPG 16 and OPG 19. Identification of cultivars and rootstocks was achievable with the use of OPT 15, OPG 19 and OPU 07 primers. In the reactions performed with 22 ISSR primers, 135 markers of pear cultivars were obtained. The size of fragments varied from 280 to 1790 bp. The greatest number of polymorphic products (9) was obtained in the reactions with primers: 830, 840 and 844. Primers 840 and 844 enabled identification of all tested genotypes. Degree of DNA polymorphism was estimated at 56.3% (RAPD) and 71.5% (ISSR). Results of the research confirm the usefulness of both techniques in identifying pear cultivars.

14

Microsatellite DNA polymorphism divergence in Chinese wheat [Triticum aestivum L.] landraces highly resistant to Fusarium head blight

63%

Wei Y. M., Hou Y. C., Yan Z. H., Wu W., Zhang Z. Q., Liu D. C., Zheng Y. L.

Journal of Applied Genetics

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2005

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tom 46

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nr 1

Genetic differences between 20 Chinese wheat (Triticum aestivum L.) landraces highly resistant to Fusarium head blight (FHB) and 4 wheat lines highly susceptible to FHB were evaluated by means of microsatellite markers, in order to select suitable parents for gene mapping studies. Thirty-nine out of 40 microsatellite markers (97.5%) were polymorphic among the 24 wheat genotypes. A total of 276 alleles were detected at the 40 microsatellite loci. The number of alleles per locus ranged from 1 to 16, with an average of 6.9 alleles. Among these microsatellite loci, the largest polymorphism information content (PIC) value was 0.914 (GWM484), while the lowest PIC value was 0 (GWM24). The mean genetic similarity index among the 24 genotypes was 0.419, ranging from 0.103 to 0.673. Clustering analysis indicated that the highly susceptible synthetic wheat line RSP was less genetically related to and more divergent from the Chinese highly resistant landraces. These results were useful in the identification of suitable parents for the development of mapping populations for tagging the FHB resistance genes among these Chinese wheat landraces.

15

The taxonomic consequences of morphological and genetic variability in Euglena agilis Carter [Euglenophyta]: species of clones in Euglena?

63%

Zakrys B.

Acta Protozoologica

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1997

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tom 36

|

nr 3

16

DNA polymorphism among barley NILs of cv. Pallas, carrying genes for resistance to powdery mildew [Blumeria graminis f.sp.hordei]

63%

Czembor P. C., Czembor J. H.

Journal of Applied Genetics

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2004

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tom 45

|

nr 2

17

Association of serum triglyceride levels with APO CIII gene Sac I [SST I] polymorphism

63%

Wlodarczyk M., Nowicka G., Walczak A.

Żywienie Człowieka i Metabolizm

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2007

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tom 34

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nr 6

Apolipoprotein CIII is a major protein component of chylomicrons and very low density lipoproteins (VLDL) and it is important in the regulation of triglyceride metabolism. Sst 1 polymorphism in the apolipoprotein CIII gene (APO CIII) has been recognized as a factor influencing levels of plasma triglycerides. We examined APO CIII Sac I (Sst I) variants and serum lipids profile in 175 subjects. DNA samples were analyzed by polymerase chain reaction (PCR) followed by Sac I (Sst I) digestion. The frequency of S2 allele was found to be three times higher among men with enhanced plasma triglyceride (TG) compared to men with normal TG levels (16,2% vs. 5,2%). Subjects with the S1S2 genotype compared to S1S1 homozygotes had higher plasma triglycerides and cholesterol levels (190 ± 155 mg/dl vs. 139 ± 90 mg/dl, p=0,035; 234 ± 60 mg/dl vs. 214 ± 37, p=0,04 respectively). These results indicate that a relationship between S2 allele of APO CIII gene and enhanced TG levels occur in studied population.

18

DNA polymorphism in various goose lines by RAPD-PCR

63%

Bednarczyk M., Siwek M., Mazanowski A., Czekalski P.

Folia Biologica

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2002

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tom 50

|

nr 1-2

19

Charakterystyka samosiewow rzepaku ozimego [Brassica napus L.] za pomoca markerow RAPD

51%

Bocianowski J., Liersch A., Bartkowiak-Broda I., Poplawska W.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2008

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nr 249

183-192

20

Identification and genetic diversity assessment of cherry cultivars and rootstocks using the ISSR-PCR technique

51%

Lisek A., Rozpara E.

Journal of Fruit and Ornamental Plant Research

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2009

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tom 17

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nr 2

95-106

The ISSR technique was used to determine the genetic similarity between 18 cultivars of sour cherry (Prunus cerasus L.), 24 cultivars of sweet cherry (Prunus avium L.) and 9 types of rootstocks for plants of these species. In reactions where 35 primers were used, 230 polymorphic DNA fragments diversifying the rootstocks were acquired, as well as 144 polymorphic fragments for the cultivars of sour cherry and 98 of sweet cherry. The highest degree of DNA polymorphism was observed in the case of the rootstocks (71.2%). For sour cherry, it was 50.7% and for sweet cherry 39.5%. It was possible to distinguish between types of rootstocks using two primers (827, 841), cultivars of sour cherry using also two primers (825, 841), whereas in order to distinguish the sweet cherry cultivars, three primers had to be used: 830, 841 and 843. Among the cultivars of sweet cherry, the highest genetic similarity was observed between 'Van' and 'Techlovan', 'Regina' and 'Karina', 'Summit' and 'Sam'. In the case of sour cherry, the most similar genetically proved to be 'Debreceni Botormo' and 'Ujfehertoi Furtos' as well as 'Nefris' and 'Safir'. Among the rootstocks, the least disparity demonstrated genotypes of two groups from the GiSeLa and PHL series. Obtained ISSR markers allow the identification of tested genotypes as well as their more accurate characterization. Results of the research may find application in gene banks of Prunus genotypes, and in orchard and nursery practice.

Ograniczanie wyników

Species identification of Diplostomum pseudospathaceum Niewiadomska, 1984 and D.paracaudum [Iles, 1959] metacercariae using DNA polymorphism amplified by arbitrary primers

DNA polymorphism of introns 1 and 2 of prolactin receptor gene and its association with litter size in goats

Silent point mutation polymorphism of the bovine CD18 encoding gene

DNA polymorphism of the alphaA-globin gene in domestic pigeon

Searching for the most useful genotypes of Lupinus mutabilis sweet for breeding purpose

PCo-A analysis of strawberry germplasm used in European breeding programs, based on evaluation of DNA polymorphism of investigated plants

The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil

Dopamine D2 receptor gene polymorphism and body mass

Badanie polimorfizmu rzepakochwastow za pomoca markerow RAPD

Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers

Analysis of DNA polymorphism [RAPD-PCR] and reciprocal effects of geese crossbreeds

Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo

Identification of pear cultivars with RAPD and ISSR markers

Microsatellite DNA polymorphism divergence in Chinese wheat [Triticum aestivum L.] landraces highly resistant to Fusarium head blight

The taxonomic consequences of morphological and genetic variability in Euglena agilis Carter [Euglenophyta]: species of clones in Euglena?

DNA polymorphism among barley NILs of cv. Pallas, carrying genes for resistance to powdery mildew [Blumeria graminis f.sp.hordei]

Association of serum triglyceride levels with APO CIII gene Sac I [SST I] polymorphism

DNA polymorphism in various goose lines by RAPD-PCR

Charakterystyka samosiewow rzepaku ozimego [Brassica napus L.] za pomoca markerow RAPD

Identification and genetic diversity assessment of cherry cultivars and rootstocks using the ISSR-PCR technique