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Przydatność liofilizowanego osocza końskiego do identyfikacji Candida albicans za pomocą metody Taschdjian

100%

Pekowski M., Szyszymar B.

Diagnostyka Laboratoryjna

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1969

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tom 05

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nr 3

2

Comparison of aspartate proteinase activity of Candida albicans strains isolated from children with and without Helicobacter pylori

100%

Rozga A., Kurnatowski M., Wasowska-Krolikowska K.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

3

The effect of pulsation magnetic field on enzymatic activity of hydrolases of Candida albicans strains isolated from patients of risk group

100%

Glowacka A., Budak A.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

4

Candida albicans Berthout, 1923: Hydrolases activity and own method of digestive tract strains biotyping

100%

Kurnatowski M., Wasowska-Krolikowska K., Kurnatowska A.

Wiadomości Parazytologiczne

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2002

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tom 48

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nr 4

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMerieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e₂ - phosphatase alcaline, e₆ – leucine arylamidase, e₁₁ - phosphatase acid, e₅ - lipase (c₁₄); e₇ - valine arylamidase, e₁₂ - naphtol-AS-BI-phosphohydrolase, e₁₆ - α-glucosidase and e₁₈ - N-acetyl-ß-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1: 4: 6: 4: 1): all „+”; one „ -” three „+”; two „-”, two ,,+”; tbree „-”, one „+”; all „-”. We bave found the following biotypes: A (16.1 ± 3.5%), B₁ (2.7 ± 2.53%), B₃ (8.0 ± 25%), B₄ (22.3 ± 3.9%), C₂ (1.8 ± 1.3%), C₃ (7.1 ± 2.4%), C₆ (30.4 ± 4.3%), D₃ (11.6 ± 3.0%).

5

Multiple drug resistance in Candida albicans

100%

Prasad R., Murthy S. K., Gupta V., Prasad R.

Acta Biochimica Polonica

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1995

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tom 42

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nr 4

By functional complementation of a PDR 5 (pleiotropic drug resistance) null mutant of S. cerevisiae, we have recently cloned and sequenced a multidrug resistance gene CDR1 (Candida Drug Resistance). Transformation by CDR1 of a PDR 5 disrupted host hypersensitive to cycloheximide and chloramphenicol resulted in resistance to these as well as other unrelated drugs. The nucleotide sequence of CDR 1 revealed that, like PDR 5, it encodes a putative membrane pump belonging to the ABC superfamily. CDR 1 encodes a protein of 169.9 kDa whose predicted structural organisation is characterised by two homologous halves, each comprising a hydrophobic region, with a set of six transmembrane stretches, preceded by a hydrophilic binding fold. We now have evidence to suggest that there are several PDR homologues present in C. albicans which display multidrug resistance and a collateral sensitivity pattern different from PDR 5 and CDR 1. The functions of such genes and their products in the overall physiology of C. albicans is not yet established.

6

Candida albicans denture biofilm and its clinical significance

86%

Serefko A. D., Poleszak E. J., Malm A.

Polish Journal of Microbiology

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2012

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tom 61

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nr 3

Fungi belonging to Candida genus, especially C. albicans play an important role in microflora of oral cavity. Microbial colonisation process taking place within oral cavity is inseparably related to formation of multispecies biofilm, i.e. dental and denture plaque. A mature fungal biofilm is a heterogeneous three-dimensional dense conglomeration of mixture of di!erent morphological forms: blastospores, germ tubes, pseudohyphae and hyphae surrounded by the extracellular polymeric matrix. Composition and specific properties of substratum, saliva and yeasts as well as multiple intricate interactions between all of them influence the ability of Candida spp. isolates to adhere and colonise both natural and artificial surfaces, followed by biofilm formation. Obviously, specific complex host-pathogen interactions also should not be neglected. A lot of additional factors like poor oral and denture hygiene, low pH under prosthesis, sufficient concentration of sugar and iron or antibody titres influence Candida adhesion and colonisation of acrylic resin base. C. albicans is capable of inducing a variety of superficial diseases of the oral mucosa. The most common clinical form of oral candidal infection related to biofilm formation a!ecting a great deal of denture wearers is denture-associated stomatitis, also known as chronic atrophic candidiasis or erythemateous candidasis. Development of C. albicans biofilm on a denture surface constitutes a difficult and hard to resolve problem which may concern every single prosthesis-wearer. Thus, careful oral and denture hygiene is highly recommended for the population of artificial teeth wearers.

7

rDNA-based genotyping of clinical isolates of Candida albicans

86%

Nawrot U., Pajaczkowska M., Wlodarczyk K., Mecler I.

Polish Journal of Microbiology

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2010

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tom 59

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nr 3

The study presents an analysis of the restriction pattern of rDNA fragments of 95 C. albicans isolates previously classified on the basis of the presence of the intron in rDNA into genotypes A (62 isolates), B (28), and C (5). Most isolates (61) with genotype A were classified as "subtype a" and one as "subtype d" (Karahan and Akar; 2005). No differences were observed in the restriction patterns of the tested genotype B isolates. Similarly, most genotype C strains (4/5) showed the same restriction pattern. The results indicate low subtyping variations of the analyzed isolates, which is in contrast to published data obtained from a Turkish collection of yeasts.

8

Ultrastructure of Candida albicans pleomorphic forms: phase-contrast microscopy, scanning and transmission electron microscopy

86%

Staniszewska M., Bondaryk M., Siennicka K., Kurzatkowski W.

Polish Journal of Microbiology

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2012

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tom 61

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nr 2

A modified method of glutaraldeyde-osmium tetroxide fixation was adjusted to characterize the ultrastructure of Candida albicans pleomorphic forms, using phase-contrast microscopy, scanning electron microscopy and transmission electron microscopy. &e discovered morphological criteria defining the individual morphotypes are discussed in terms of mycological and histopathological diagnostics of candidiasis. &e relations are discussed between fungal pleomorphism, virulence and susceptibility of di!erent morphotypes to fungicides.

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Ocena stopnia przyczepnosci Candida albicans do wybranych tworzyw akrylowych

86%

Bregula L., Trzeciak H., Nolewajka-Lasak I.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 1

67-71

W pracy zbadano czy występują różnice w adhezji wzorcowego szczepu Candida albicans АТСС 10231 do wybranych tworzyw akrylowych stosowanych w protetyce stomatologicznej. Badania przeprowadzono in vitro celem zachowania jednakowych parametrów dla każdego z materiałów. Uzyskane wyniki wskazują na różny stopień adhezji Candida albicans do poszczególnych tworzyw zarówno w przypadku powierzchni w ygładzonej jak i nie wygładzonej.

10

Tempo wzrostu Candida albicans [Robin, 1853] i Candida stellatoidea [Martin et Jones, 1938] izolowanych z ukladu oddechowego czlowieka

86%

Dynowska M.

Wiadomości Parazytologiczne

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1993

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tom 39

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nr 4

383-390

The study comprises an analysis of the growth rate of C. albicans and C. stellatoidea strains isolated from the respiratory system and incubated on Nickerson's and Sabouraud's agar in the dark and by light. The property to form hyphae and pseudohyphae by both species was used to evaluate the growth rate. The increase in their growth allowed to isolate the stages characteristic for growth and development of most fungi. Disturbances appeared with the fungi exposed to light which is the factor definitely setting back the growth. Faster growth and development was observed with C. stellatoidea.

11

Inhibitory effects of different medicinal plants on Candida albicans growth

86%

Tambur Z., Cenic Milosevic D., Mileusnic I., Doder R., Marjanovic M., Miljkovic Selimovic B., Kulisic Z., Opacic D.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 07

The objective of this study was to evaluate the in vitro antifungal properties of ethanol extracts and essential oils of medicinal plants from Serbia against Candida albicans (C. albicans) ATCC 10231. Ethanol extracts of fifteen plants were investigated, and their effects were compared with those of three different essential oils. The sensitivity of C. albicans to all plants was tested by the agar dilution method. The assay plates were estimated to contain 300, 150, 75, and 37.5 µg/ml of active extracts and 100, 50, 25, and 12.5 µg/ml of active essential oils. Inocula were applied to agar surfaces, giving approximately 106 cfu/ml of C. albicans. No inhibitory effects were observed for ethanol extracts of Hypericum perforatum and Salvia officinalis (MIC > 300 µg/ml). The most effective were the ethanol extract of Aesculus hippocastanum (MIC = 37.5 µg/ml) and the essential oil of Satureja kitaibelii (MIC = 12.5 µg/ml). Other plants showed MIC from 25 to 300 µg/ml. As far as we know, the inhibitory effects of these medicinal plants against the reference strain of C. albicans have not been commonly investigated in our country. Although the essential oil of Satureja kitaibelii shows strong activity against C. albicans, these results need clinical evaluation.

12

Fungistatic properties of glucosinolates - a reconnaissance study

86%

Goralska K., Dynowska M., Ciska E.

Polish Journal of Environmental Studies

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2009

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tom 18

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nr 3

377-382

The aim of our study was to investigate fungistatic properties of glucosinolates from seeds of four cruciferous plants against Candida albicans, obtained from different sections of the gastrointestinal and respiratory tracts. The examined extracts were observed to exert different effects on fungi depending on the site of their isolation. The strongest antimycotic activity was reported for the extract obtained from seeds of broccoli – the extract appeared more effective than fluconazole. In contrast, upon the activity of white mustard seeds extract the size of the growth inhibition zone was similar to that yielded by fluconazole. Promising results of our experiments suggest the need to further investigate the field.

13

Effect of different drying conditions for the viability of Candida albicans present on carriers

86%

Rohm-Rodowald E., Chojecka A., Jakimiak B., Wiercinska O., Ziemba B., Kanclerski K.

Roczniki Państwowego Zakładu Higieny

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2014

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tom 65

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nr 1

Background. Problems in substantial under recovery of Pseudomonas aeruginosa and Candida albicans from carriers have been demonstrated for laboratories performing phase 2, step 2 efficacy tests on disinfectants relative to levels required by the EN 13697 standard. It was thus necessary to determine recoveries of these microorganisms following procedural losses incurred during drying and to optimise drying conditions such that recoveries then complied with the standard. Objectives. The aim of the study was to establish optimal drying conditions for the recovery of Candida albicans ATCC 10231 from carriers. Materials and Methods. The evaluation was performed according to the EN 13697:2001 standard procedure. A test suspension of Candida albicans and interfering substance were inoculated onto the surface of carriers (2 cm diameter stainless steel discs) and then dried under different conditions consisting of: a 37°C incubation with and without an incubator fan as well as at 23°C (room temperature) in a laminar air flow cabinet. Carriers were dried until the surfaces appeared visibly dry and the number of surviving organisms then recovered from the surface were quantified. The following were calculated for colony forming units (cfu); N (log10 cfu in a 0.05 ml test suspension), NC (the control log10 cfu in neutralizing medium), Nts (cfu numbers remaining on the surface) and the N-NC difference which should not exceed 2 log10 when microorganism recoveries are adequate and without any toxicity effects of the neutralising medium. Experiments was conducted using validating procedure (NC) which is performed with distilled water. Results. Drying at 37°C adversely affected the survival of Candida albicans and prevented the levels of microbial recovery from carriers to reach those specified by the EN 13697 standard. However, drying at around room temperatures of 23°C reduced Candida albicans mortality and increased recoveries from the carrier to levels compliant with the standard, where the N-NC differences were not greater than 2 log10. Conclusions. The viability of Candida albicans ATCC 10231 is sufficiently improved when carriers are dried at 23°C, even if the drying time exceeds 60 minutes. The density of the initial test suspension (N) should also be increased.

14

In vitro study of secreted aspartyl proteinases Sap1 to Sap3 and Sap4 to Sap6 expression in Candida albicans pleomorphic forms

86%

Staniszewska M., Bondaryk M., Siennicka K., Kurek A., Orlowski J., Schaller M., Kurzatkowski W.

Polish Journal of Microbiology

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2012

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tom 61

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nr 4

Transition from round budding cells to long hyphal forms and production of secreted aspartic proteases (Saps) are considered virulenceassociated factors of Candida albicans. Although plenty of data dealing with Saps involvement in the infection process have been published, Saps expression by the different pleomorphic forms as well as the capacity of C. albicans filaments to express Sap1-6 under serum influence are poorly investigated. In this study, we used immunofluorescence and immunoelectron microscopy for the detection of Sap1-6 isoenzymes in C. albicans pleomorphic cells (blastoconidia, germ tubes, pseudohyphae, true hyphae) grown in Sap-inductive human serum and Sap non-inductive medium – yeast extract-peptone-glucose (YEPD). Isoenzymes were below the detection level in all blastoconidial cells grown in YEPD for 18 h. Sap1-6 expression was hardly detected in C. albicans cells cultivated in serum for 20 min. Increasing level of Sap1-6 expression was observed when C. albicans was incubated for 2, 6 and 18 h in serum corresponding to the development of germ tubes, pseudohyphae and true hyphae. !e expression of Sap1-3 in pseudohyphae and true hyphae was more intensive compared to Sap4-6. !us, we could show that human serum induced hyphae formation and the expression of Sap1-6 were co-regulated.

15

Emodin reduces the activity of (1,3)-beta-D-glucan synthase from Candida albicans and does not interact with caspofungin

86%

Janeczko M.

Polish Journal of Microbiology

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2018

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tom 67

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nr 4

Candidiasis is the most common opportunistic yeast infection, with Candida albicans as a paramount causative species. (1,3)-β-D-glucan is one of the three main targets of clinically available antifungal agents used to treat Candida infections. It is one of the most abundant fungal cell wall components. Echinocandins represent the newest class of antifungals affecting cell wall biosynthesis through non-competitive inhibition of (1,3)-β-D-glucan synthase. Therefore, treatment with echinocandins causes defects in fungal cell integrity. In the present study, similar activity of emodin (6-methyl-1,3,8-trihydroxyanthraquinone) has been revealed. Many reports have already shown the antifungal potential of this pleiotropic molecule, including its activity against C. albicans. The aim of this report was to evaluate the activity of emodin towards a new molecular target, i.e. (1,3)-β-D-glucan synthase isolated from Candida cells. Moreover, given the identical mechanism of the activity of both molecules, interaction of emodin with caspofungin was determined. The study revealed that emodin reduced (1,3)-β-D-glucan synthase activity and increased cell wall damage, which was evidenced by both a sorbitol protection assay and an aniline blue staining assay. Furthermore, the synergy testing method showed mainly independence of the action of both tested antifungal agents, i.e. emodin and caspofungin used in combination.

16

Selected parameters of local immunity and properties of fungi [between and within species] isolated from palatine tonsils

86%

Kurnatowski P., Jaskolowska A., Rozga A.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

17

Antifungal activity of 2-[4-chlorophenyl]-1,2-benzisoselenazol-3[2H]-one, the analog ebselen

86%

Bien M., Blaszczyk B., Kalinowska K., Mlochowski J., Inglot A. D.

Archivum Immunologiae et Therapiae Experimentalis

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1999

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tom 47

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nr 3

18

Evaluation of adhesive properties of Candida albicans isolated from the oral cavity in HIV positive patients

86%

Macura A. B., Bort A.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 4

The aim of the work was to compare adhesive properties of Candida albicans strains isolated from the oral cavity in HIV+ vs. HIV- persons. The materials were Candida albicans strains and buccal epithelial cells isolated from both HIV+ and HIV- persons. We applied the in vitro adherence test, primarily described by Kimura and Pearsall and modified by Macura. The strongest adherence was found between both fungi and epithelial cells isolated from a HIV+ person. The adherence of C. albicans isolated from HIV+ patients was significantly stronger to epithelium collected from HIV+ than HIV- persons.

19

QSARs of some novel isosteric heterocyclics with antifungal activity

86%

Yalcin I., Oren I., Temiz O., Sener E. A.

Acta Biochimica Polonica

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2000

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tom 47

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nr 2

QSAR analysis of a set of previously synthesized 2,5,6-trisubstituted benzoxazole, benzimidazole and 2-substituted oxazolo(4,5-b)pyridine derivatives tested for growth inhibitory activity against Candida albicans, was performed by using the computer-assisted multiple regression procedure. The activity contributions for either heterocyclic ring systems or substituent effects of these compounds were determined from the correlation equation and the predictions for the lead optimization were described. The resulting QSAR revealed that the oxazolo(4,5-b)pyridine ring system with the substitution of a benzyl moiety at position 2 was the most favourable structure among the heterocyclic nuclei. Moreover, the fifth position in the fused ring system is found more significant than the other positions in improving the activity.

20

Comparative studies on cell stimulatory, permeabilizing and toxic effects induced in sensitive and multidrug resistant fungal strains by amphotericin B [AMB] and N-methyl-N-D-fructosyl amphotericin B methyl ester [MFAME]

86%

Szlinder-Richert J., Cybulska B., Grzybowska J., Borowski E., Prasad R.

Acta Biochimica Polonica

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2000

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tom 47

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nr 1

N-Methyl-N-D-fructosyl-amphotericin B methyl ester (MFAME) is a new derivative of amphotericin B, which is characterised by low toxicity to mammalian cells and good solubility in water of its salts. The antifungal activity and effects of MFAME towards Candida albicans and Saccharomyces cerevisiae multidrug resistant MDR(+) and sensitive MDR(-) strains was compared with those of parent compound. The results obtained indicate that MDR(+) S. cerevisiae was sensitive to MFAME as well as to AMB. MFAME exhibited the same effects on fungal cells studied as parent antibiotic. The two antibiotics, depending on the dose applied induced cell stimulation, K+ efflux, and/or had a toxic effect.

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