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The purpose of this study was to determine the in vitro susceptibility to 6 antifungal agents of 143 strains of Candida species isolated from 545 patients with suspicion of fungal infection. Test ATB Fungus (bioMerieux) was used. Among all Candida isolates 91.6% were susceptible to tested polyenes and 32.9% to all azoles. Less susceptible to polyenes isolates were noted in low percentage (0.7 to 4.2%) and to azoles more frequcntly (32.9 to 41.2%). Candida isolates resistant to antifungal agents tested were dependent from the kind of clinical samples and species of fungi. Monitoring of the susceptibility to antifungal agents by ATB commercial test seems to be uscful tool for therapeutic purposes.
Candida sp. may be regarded as one of the leading etiologic agents of hospital-acquired infections, including those related with the indwelling medical devices, which become colonized by the yeasts, accompanied by biofilm formation. In this paper we assayed in vitro susceptibility to caspofungin of planktonic and sessile cells of nasopharyngeal isolates of Candida sp. Two types of biomaterials were used - silicone elastomer-coated latex urinary Foley catheter and PCV Thorax catheter. The minimal inhibitory concentrations (MIC) of caspofungin for planktonic Candida sp. cells ranged from 0.008 to 0.031 mg/l, while the minimal fungicidal concentrations (MFC) from 0.008 to 0.062 mg/l, with MFC/MIC ratios ≤2. The minimal concentration of caspofungin preventing adhesion process of Candida sp. on both biomaterials ranged from 0.004 to 0.031 mg/l, while preventing biofilm formation from 0.004 to 0.062 mg/l. In contrast, much higher minimal concentrations of caspofungin were needed to eradicate the mature biofilm (0.25 to >8 mg/l). In all cases, drug concentrations depended on the strain and the biomaterial used. Our preliminary data suggest that caspofungin, showing good anti-adherent activity in vitro against Candida sp., appears to be a potential agent rather for prophylaxis of the yeast infections associated with biomaterials but not for their treatment.
Increasing use of elctromagnetic fields (EF) in the treatment of various diseases may have potential impacts on fungi - possible aetiological factors of concomitant mycoses. The aim of this study was to investigate the effects of EF on miconazole susceptibility among fungi of the genus Candida showing confirmed pathogenicity in humans. Fifteen Candida strains obtained from patients were used and their susceptibility to miconazole was determined by diffusion in agar gel. Antifungal activity of miconazole was assessed as minimal inhibitory concentration (MIC) which was calculated for Candida strains not exposed to EF (control) and EF-exposed (3 experiments). In the majority of cases the susceptibility of Candida species to miconazole decreased (higher MICs) after the first week of EF exposure, regardless of the EF's parameters. This was followed by an increase in susceptibility (lower MICs) after the second week of exposure to EF of 2 mT intensity and frequency 3 Hz (experiment I) and an EF of 9 mT intensity and 12.5 Hz frequency (experiment II) relative to control. An increase in susceptibility (lower MICs) was observed in the second week of exposure, whatever the parameters of the EF. The application of low intensity, low frequency EF for a period of at least two weeks may be beneficial in the treatment of mycoses caused by pathogenic fungi of Candida genus.
The human body is composed of 1014 cells, of which only 10% of them belong to the human host itself: the remaining 90% are microorganisms. Commensal microorganisms are necessary for the proper functioning of the human body and covers an area that could potentially become sites of adhesion of pathogenic microorganisms, it thus represents a form of competition for potential pathogens. The coexistence of fungi and bacteria in cases of systemic infections is a significant diagnostic and therapeutic problem, and the human immune system reacts differently, depending on the pathogen. Numerous publications exist concerning the relationship between microorganisms belonging to different ecological groups, the majority of which concern the interaction between macro-organisms and potential pathogens, or the synergistic relationship between parasitic species. However, there is still too little information concerning the role of natural microbiota in maintaining homeostasis and the relationships between particular species inhabiting the human organism.
Chorzy po zabiegach chirurgicznych, żywieni pozajelitowe, są szczególnie narażeni na zakażenie grzybicze o etiologii Candida. Dokonano analizy mikologicznej próbek materiałów klinicznych pozyskanych od chorych żywionych pozajelitowe, a także od chorych, u których nie zastosowano tej metody leczenia. W materiale klinicznym pochodzącym od chorych żywionych pozajelitowe, dominującym gatunkiem była Candidaglabrata. W posiewach materiałów klinicznych pozyskanych od chorych nie otrzymujących żywienia pozajelitowego dominował gatunek Candida albicans
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