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Background. Commonly occurring diseases can have the origin in oxidative processes ongoing in the human body. Vegetables of Brassicaceae family are the essential sources of natural antioxidants, especially phenolic compounds, in the human diet. The research was aimed to estimate the content of phenolic compounds in selected vegetables and their quantity changes during hydrothermal processes. Material and methods. The vegetables subjected to analysis were: kale, broccoli, Brussels sprouts, and white and green cauliflower. The fresh and processed (blanched, cooked, frozen, cooked after freezing) vegetables were freeze-dried. The levels of phenolic acids and flavonols by HPLC method were estimated. Results. The presence of derivatives of hydroxycinnamic acid, mainly of caffeic acid, p-coumaric acid, sinapic acid and of flavonols - kaempferol, and in smaller amounts of quercetin was found. The largest amounts of above components were present in kale (total 94.4 mg-100 g'1 of fresh matter), whereas the smallest amounts were found in white and green cauliflower - 3.6 mg-100 g’1 f.m. and 3.03 mg-100 g'1 f.m., respectively. The applied technological processes contributed to lover amounts of all tested compounds depending on the process and the vegetable kind. The biggest loses, up to 70-80%, took place during cooking of raw and previously frozen vegetables. Conclusions. Analysed Brassicaceae were characterized by high contents of the investigated flavonoids. The best source of those compounds was kale whereas the smallest amounts of searched components were presented in cauliflowers. The used hydrothermal processes led to losses of searched compounds.
Several factors, including growth medium, inoculum density, and inoculum storage affected the reaction of resistant and susceptible Brassicas to Plasmodiophora brassicae in the greenhouse. A high level of disease was achieved using Peat-litte mix R and a commercial greenhouse mix. There was little difference in disease incidence when spore suspensions were pipeted into planting holes or when seedlings were dipped into spore suspensions. Seedlings transplanted from sand or Petri dishes gave higher levels of disease than direct seeding. Two-year frozen storage of clubs reduced the inoculum potential to a level unable to define resistance. Inoculum levels of 10³⁻⁷ spores per ml from fresh clubs, or 10⁵⁻⁷ spores per ml from clubs frozen for 2 or 4 years, produced 90% club incidence of susceptible cauliflower and Chinese cabbage. A concentration of only 10⁶⁻⁸ spores per ml from fresh clubs was required for maximum disease expression in a cauliflower line partially resistant to clubroot.
The micromorphological typology of seed surfaces was investigated in rapid-cycling Brassica (RCBr) forms, using scanning electron microscopy. Four types of basic ornamentation pattern were recognized: reticulate (B. rapa, B. juncea), reticulate-foveolate (B. nigra), randomly reticulate (B. oleracea, B. napus) and reticulate-rugose (B. carinata). The seed coats showed variation in the shape and size of the testa epidermal cells and the structure of the outer periclinal and anticlinal cell walls. The surface patterns in RCBr seeds were less exposed and were moderately reticulated compared to cultivated Brassica species. The micromorphological characteristics of the seed coats may provide valuable additional diagnostic criteria for delimitation of RCBr forms, and can be used in identification of seeds.
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