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Detection of anti-Borrelia garinii antibodies in diagnosis of Lyme borreliosis

100%

Flisiak R., Prokopowicz D.

Wiadomości Parazytologiczne

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1998

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tom 44

|

nr 3

2

Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic)

84%

Venclikova K., Betasova L., Sikutova S., Jedlickova P., Hubalek Z., Rudolf I.

Acta Parasitologica

|

2014

|

tom 59

|

nr 4

A total of 1279 field-collected Ixodes ricinus ticks were screened for Borrelia burgdorferi sensu lato (s.l.) in a natural and an urban ecosystem of Ostrava city (Czech Republic) by using molecular methods. Minimal prevalence rate for Borrelia burgdorferi s.l. in ticks for the urban park Bělský les was found to be 13.8% (17.6% in males, 17.8% in females and 11.7% in nymphs), similarly for the natural site Proskovice was minimal prevalence 15% (12.5% in males, 20% in females and 14.9% in nymphs). Six proven human pathogenic genomic species have been recorded in the study: B. afzelii, B. garinii, B. burgdorferi s.s., B. valaisiana, B. lusitaniae, and B. spielmanii. Emerging B. spielmanii was detected for the first time in Ixodes ricinus ticks in the region. Our results highlight the need for surveillance of zoonotic tick-borne pathogens even in urban areas.

3

Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin Region [Eastern Poland]

84%

Cisak E., Wojcik-Fatla A., Stojek N. M., Chmielewska-Badora J., Zwolinski J., Buczek A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2006

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tom 13

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nr 2

The objective of the study was to determine the prevalence rate of 3 Borrelia burgdorferi genospecies in Ixodes ricinus ticks collected from wooded areas of the Lublin region (eastern Poland). A group of 1,813 I. ricinus ticks from 6 districts were examined for the presence of Borrelia burgdorferi sensu lato (B.b. s.l.) by polymerase chain reaction (PCR). Another group of 438 I. ricinus ticks collected from 4 districts were examined for the presence of B.b. s.l. by culture on BSKH liquid medium confirmed by PCR, and for the presence of Borrelia spp. by dark field microscopy (DFM). Borrelia burgdorferi genospecies (Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii) were determined by nested-PCR in 113 ticks lysates showing presence of B.b. s.l. (in PCR or in culture and PCR). 5.4% of I. ricinus ticks examined by PCR showed the presence of B.b. s.l. DNA. The infection rate was highest in females (12.1%), lower in males (6.0%) and the lowest in nymphs (1.7%) (p<0.001). The minimum infection rate of I. ricinus ticks with B.b. s.l. determined by culture was 3.4%, whereas the minimum infection rate of ticks with motile spirochetes morphologically resembling Borrelia spp., determined by DFM, amounted to 11.2%. The presence of all 3 Borrelia burgdorferi genospecies under investigation was found in ticks collected from 5 out of 6 examined districts. In 81.4% of infected ticks only single infection with 1 genospecies was observed, while coinfections with 2 or 3 genospecies were detected respectively in 16.8% and 1.8% of infected ticks. Borrelia burgdorferi sensu stricto was the dominant genospecies in all examined tick stages and districts, both in single infections and in coinfections, and found in a total of 62.8% of I. ricinus ticks infected with B.b. s.l. Borrelia afzelii and Borrelia garinii were less frequent and observed in respectively 39.8% and 17.8% of infected ticks.

4

The preference of sexual transmission of Borrelia garinii by males of Ixodes persulcatus ticks [Acari, Ixodidae]

84%

Alekseev A. N., Dubinina H. V., Rijpkema S. G. T., Schoul L. M.

Wiadomości Parazytologiczne

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1998

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tom 44

|

nr 3

5

Phylogenetic diversity of flaB gene among Borrelia spp. in Ixodes ricinus ticks from urban and semi-natural habitats

67%

Kowalec M., Szewczyk T., Welc-Faleciak R.

Annals of Parasitology

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2016

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tom 62

|

nr Suppl.

6

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

67%

Stefancikova A., Derdakova M., Stepanova G., Pet'ko B., Szestakova E., Skardova I., Cislakova L.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

|

nr 2

211-216

Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B31 and Ir112, B 31 and K 24 differed statistically significantly ( test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K 24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir 105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04% The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close strains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.

7

Ekologia boreliozy z Lyme - rola ladowych zwierzat kregowych

67%

Gryczynska A.

Wiadomości Ekologiczne

|

1997

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tom 43

|

nr 3

207-222

8

Risk of tick-borne bacterial diseases among workers of Roztocze National Park [South-Eastern Poland]

67%

Cisak E., Chmielewska-Badora J., Zwolinski J., Wojcik-Fatla A., Polak J., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2005

|

tom 12

|

nr 1

The objective of the present study was to assess the risk of borreliosis and anaplasmosis (ehrlichiosis) among the forestry workers of the Roztocze National Park (south-eastern Poland) by examination of Ixodes ricinus ticks living in park area with PCR method, and by the serological and clinical examination of the workers. In 406 examined ticks, the prevalence of infection with Borrelia burgdorferi sensu lato was 11.5%. The nested PCR reaction for determining the genospecies showed that the most common was Borrelia burgdorferi sensu stricto (55.3% of total positive) followed by Borrelia afzelii (38.3%). As many as 6.1% out of 115 examined ticks showed the presence of Anaplasma phagocytophilum DNA. The infection rate was high in males and females (14.3% and 11.1% respectively) and low in nymphs (1.5%). In 46 out 113 examined forestry workers (40.7%) the presence of specific IgG and/or IgM antibodies against Borrelia burgdorferi sensu lato was found in ELISA test, while only 4 out of 56 urban blood donors showed a positive response (p<0.0001).The prevalence of IgG antibodies against Anaplasma phagocytophilum determined with the use of indirect immunofluorescence test (IFA) was 17.7% in forestry workers compared to 5.4% in reference group of blood donors (p<0.05). No correlation was found between the presence of antibodies to A. phagocytophilum and to B. burgdorferi s. l. Clinical investigations of 113 forestry workers showed 3 cases of borreliosis (2.7%) and no cases of anaplasmosis (ehrlichiosis). In conclusion, forestry workers of the Roztocze National Park in south-eastern Poland are often exposed to Ixodes ricinus ticks infected with Borrelia burgdorferi and Anaplasma phagocytophilum, and show a high proportion of asymptomatic borreliosis and anaplasmosis (ehrlichiosis) manifested by a positive serologic response, while the number of clinical cases is relatively low.

9

Epidemiological survey of human borreliosis diagnosed in Eastern Slovakia

67%

Stefancikova A., Pet'ko B., Rozicka I., Salyova N., Ohlasova D.

Annals of Agricultural and Environmental Medicine

|

2001

|

tom 08

|

nr 2

10

Positive findings of Borrelia burgdorferi in Culex [Culex] pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method

67%

Zakovska A., Nejedla P., Holikova A., Dendis M.

Annals of Agricultural and Environmental Medicine

|

2002

|

tom 09

|

nr 2

11

ELISA comparison of three whole-cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Kosice, eastern Slovakia

67%

Stefancikova A., Tresova G., Pet'ko B., Skardova I., Sesztakova E.

Annals of Agricultural and Environmental Medicine

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1998

|

tom 05

|

nr 1

12

Identification and characterization of 31 isolates of Borrelia burgdorferi [Spirochaetales, Spirochaetaceae] obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis

67%

Zakovska A., Janouskovcova E., Pejchalova K., Halouzka J., Dendis M.

Acta Parasitologica

|

2008

|

tom 53

|

nr 2

Borrelia burgdorferi sensu lato, the etiologic agent of Lyme borreliosis, circulates between ticks and vertebrate hosts. Two main genospecies typically occur in the Czech Republic Borrelia garinii and Borrelia afzelii, transmitted generally by Ixodes ricinus (L., 1758) ticks. The aim of our study was to identify spirochaete isolates focusing on Borrelia burgdorferi acquired from different sources: vectors (ticks), potential vectors (mosquitoes, small mites) and hosts (wild rodents). In the years 1996–2001 a total of 2398 ticks, 72 mites (from wild rodents), 2700 mosquito adults, 1798 mosquito larvae and organ parts (kidney and spleen) of 216 wild rodents were collected from seven localities in the Czech Republic. A total of 31 spirochaete strains were isolated: 13 strains from ticks, 1 strain from mite (Haemogamasus sp.), 15 strains from rodents, 1 strain from mosquito adults and 1 strain from mosquito larva. For the genospecies identification of these isolates PCR, PCR-RFLP was used and their characterization was also performed by SDS-PAGE. By nested PCR method all except one isolated strains were detected as Borrelia burgdorferi s.l. Following PCR-RFLP molecular analysis results, tick isolates were identified as B. garinii and B. afzelii, the strain isolated from the mite was identified as B. afzelii. This is the first isolated strain of B.b.s.l. from a different mite of infraorder Parasitiformes than tick. All of rodent isolates were identified as B. afzelii; mosquito adult isolate was identified as B. afzelii. Larval isolate from mosquito is spirochaete, but does not belong to Borrelia burgdorferi sensu lato group.

13

Wspolwystepowanie Borrelia garinii i B. afzelii wsrod populacji gryzoni lesnych

59%

Pawelczyk A., Sinski E.

Wiadomości Parazytologiczne

|

2001

|

tom 47

|

nr 4

741-746

The maintenance of Borrelia burgdorferi s.1. in the environment is dependent on the zoonotic cycle involving tick vectors and certain reservoir hosts. It is well known, that the same species of wild rodents, as well as the vector Ixodes ricinus, are often co-infected with at least two genomospecies of B. burgdorferi s.1.: B. afzelii and B. garinii. The ticks collected from two rodent species: Clethrionomys glareolus and Apodemus flavicollis were examined for the presence of B. burgdorferi s.1., as well as for B. garinii and B. afzelii. In this study, an immunofluorescent antibody assay (lFA) and polymerase chain reaction (PCR) protocols were used. The high level of infestation in rodents (90% for C. glareolus and nearly 100% for A. flavicollis) shows that wild rodents are important hosts of the immature stages of I. ricinus. A high percent of Borrelia positive ticks collected from bank voles and yellow necked mice; above 7% determined by 1FA and 2% determined by PCR, elearly revealed that these species of animals are competent zoonotic reservoirs of B. burgdorferi s.l.

Ograniczanie wyników

Detection of anti-Borrelia garinii antibodies in diagnosis of Lyme borreliosis

Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic)

Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin Region [Eastern Poland]

The preference of sexual transmission of Borrelia garinii by males of Ixodes persulcatus ticks [Acari, Ixodidae]

Phylogenetic diversity of flaB gene among Borrelia spp. in Ixodes ricinus ticks from urban and semi-natural habitats

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

Ekologia boreliozy z Lyme - rola ladowych zwierzat kregowych

Risk of tick-borne bacterial diseases among workers of Roztocze National Park [South-Eastern Poland]

Epidemiological survey of human borreliosis diagnosed in Eastern Slovakia

Positive findings of Borrelia burgdorferi in Culex [Culex] pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method

ELISA comparison of three whole-cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Kosice, eastern Slovakia

Identification and characterization of 31 isolates of Borrelia burgdorferi [Spirochaetales, Spirochaetaceae] obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis

Wspolwystepowanie Borrelia garinii i B. afzelii wsrod populacji gryzoni lesnych