Wyniki wyszukiwania

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In vitro cultivation of Babesia bovis in a modified microaerophilous stationary phase [MASP] culture system

100%

Mishra A. K., Clabaugh G., Kakoma I., Ristic M.

Acta Parasitologica

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1992

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tom 37

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nr 1

2

End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle

63%

Charaya G., Rakha N. K., Kumar A., Maan S., Goel P.

Acta Parasitologica

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2021

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tom 66

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nr 1

3

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

63%

Liu J., Guan G., Liu A., Li Y., Yin H., Luo J.

Acta Parasitologica

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2014

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tom 59

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nr 1

In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.

4

Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA)

51%

Yang C., Liu J., Li A., Li Y., Liu A., Xie J., Liu G., Yin H., Guan G., Luo J.

Acta Parasitologica

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2015

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tom 60

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nr 4

5

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Babeszjoza u bydla

51%

Sawczuk M.

Wiadomości Parazytologiczne

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2007

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tom 53

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nr 2

73-79

6

Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes

51%

Mishra A. K., Reddy G. G. B., Rao J. R., Tewari A. K.

Acta Parasitologica

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1998

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tom 43

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nr 1

An affinity purified Babesia bigemina piroplasm antigen was successfully employed in a Dot-ELISA for detection of antibodies in buffaloes and catle. A low degree of cross-reactivity was seen against putative B. bigemina (Mexican) and B. bovis undiluted reference serum and no cross-reactivity was observed in seru dilutions of 1:10 and above. Theileria annulata did not show any reactivity with B. bigemina. The assay detected antibodies at 7, 14 or 21 days post-experimental infection in bovine calves with 37.5, 87.5 and 100% seroreactivity, respectively. The incidence of B. bigemina infections was found highest in cattle (19.91) as against 7.4% in buffaloes out of 486 sera samples, examined from Boophilus microplus infested areas from Orissa, Rajasthan and Uttar Pradesh States.

7

W kleszczach strachu

45%

Welc-Faleciak R.

Wiedza i Życie

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2012

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nr 05

Ograniczanie wyników

In vitro cultivation of Babesia bovis in a modified microaerophilous stationary phase [MASP] culture system

End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA)

Babeszjoza u bydla

Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes

W kleszczach strachu