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Dying clematis leaves were observed during a two-year study (2011–2012) conducted in the city of Krakow, Poland. The dying leaves showing symptoms of necrosis contained 46 fungi species. Alternaria alternata and Epicoccum nigrum dominated in the clematis phyllosphere. Arthinium phaeospermum, Boeremia exigua, Botrytis cinerea, Chaetomium globosum, Cladosporium cladosporioides, Fusarium oxysporum, Gibberella baccata, G. tricincta, Penicillium expansum, Pestalotiopsis sydowiana, Phoma glomerata, Ph. medicaginis, Pleurostomophora richardsiae and others occurred in large numbers. The dynamics of leaf colonization by fungi causing necrosis increased during the vegetation period. In November 2012, over 60% more of the fungous colonies were isolated in comparison with the number isolated in July. The most infected plants grew nearer to the acoustic screen. Kacper and Polish Spirit were the most infected cultivars, whereas Etoile Violette and Maidwell Hall were the least infected. The occurrence of fungi resulted in the considerable worsening of the decorative values of the plants.
For the last decade there have been considerable discussion concerning the linearity / non-linearity of the oxygen uptake (O2) - power output (W) relationship with strong experimental evidence of non-linearity provided mainly by breath-by-breath measurements. In this study, we attempted to answer the question whether the O2 - W relationship in the Åstrand nomogram, as presented in the Textbook of Work Physiology, P.-O. Åstrand et al. (2003), page 281, based on the Douglas bag method, is indeed linear, as stated by the authors before, or if a change point in O2, described by Zoladz et al. (1998) Eur J Appl Physiol 78: 369-377, can possibly be detected in those data. The O2 - W data were taken from the Åstrand nomogram referenced above and from the Table 9.5 on page 282 in the same reference and tested for the presence of the change point in O2, using our two-phase model (see the reference above). In the first phase, a linear O2 - W relationship was assumed, whereas in the second one (above the so-called change point) an additional increase in O2 above the values expected from the linear model was allowed. It was found that in the data taken from the Åstrand nomogram (data for men), as well as in the data taken from the Table 9.5, statistically significant change points in O2 were present at the power output of 150 W. The documentation of the presence of a change point in the O2 - W relationship in the Åstrand data provides further evidence for the existence of a non-linearity in the O2 - W relationship in incremental exercise tests of humans, also in O2 data based upon the Douglas bag method.
 Both ADAM17, the secretase responsible for the shedding of ectodomains of numerous membrane proteins including TNF and its receptors, as well as nitric oxide synthesized by inducible nitric oxide synthase play regulatory roles in inflammation and tumor progression. We analyzed the effect of endogenous and exogenous nitric oxide on the expression and activity of ADAM17 in murine endothelial cells and a monocyte/macrophage cell line. We found that endogenous nitric oxide influenced neither ADAM17 mRNA level nor the shedding of two ADAM17 substrates, TNF and TNFR1. Exogenous NO significantly diminished the release of TNF and TNFR1 without affecting the ADAM17 transcript level. Our data seem contrary to a previous report that showed the activation of ADAM17 by nitric oxide (Zhang et al., 2000, J Biol Chem 275: 15839-15844). We discuss potential mechanisms of NO-mediated inhibition of ectodomain shedding and possible reasons of discrepancy between our results and the previous report.
The aim of this study was to examine the relationship between the content of various types of myosin heavy chain isoforms (MyHC) in the vastus lateralis muscle and pulmonary oxygen uptake during moderate power output incremental exercise, performed at low and at high pedalling rates. Twenty one male subjects (mean ± SD) aged 24.1 ± 2.8 years; body mass 72.9 ± 7.2 kg; height 179.1 ± 4.8 cm; BMI 22.69 ± 1.89 kg . m-2; VO2max 50.6 ± 5.3 ml . kg . min-1, participated in this study. On separate days, they performed two incremental exercise tests at 60 rev . min-1 and at 120 rev . min-1, until exhaustion. Gas exchange variables were measured continuously breath by breath. Blood samples were taken for measurements of plasma lactate concentration prior to the exercise test and at the end of each step of the incremental exercise. Muscle biopsies were taken from the vastus lateralis muscle, using Bergström needle, and they were analysed for the content of MyHC I and MyHC II using SDS - PAGE and two groups (n=7, each) were selected: group H with the highest content of MyHC II (60.7 % ± 10.5 %) and group L with the lowest content of MyHC II (27.6 % ± 6.1 %). We have found that during incremental exercise at the power output between 30 - 120 W, performed at 60 rev . min-1, oxygen uptake in the group H was significantly greater than in the group L (ANCOVA, p=0.003, upward shift of the intercept in VO2 / power output relationship). During cycling at the same power output but at 120 rev . min-1, the oxygen uptake was also higher in the group H, when compared to the group L (i.e. upward shift of the intercept in VO2 / power output relationship, ANCOVA, p=0.002). Moreover, the increase in pedalling rate from 60 to 120 rev . min-1 was accompanied by a significantly higher increase of oxygen cost of cycling and by a significantly higher plasma lactate concentration in subjects from group H. We concluded that the muscle mechanical efficiency, expressed by the VO2 / PO ratio, during cycling in the range of power outputs 30 - 120 W, performed at 60 as well as 120 rev . min-1, is significantly lower in the individuals with the highest content of MyHC II, when compared to the individuals with the lowest content of MyHC II in the vastus lateralis.
In this study we have examined the relationship between the content of different isoforms of MyHC in the vastus lateralis m. quadricipitis femoris and the VO2 / power output relationship during incremental cycling exercise. Twenty-one male subjects: aged 24.0 ± 2.5 years, body mass 73.0 ± 7.2 kg, height 179 ± 5 cm, BMI 22.78 ± 1.84 kg . m-2 , VO2 max 3697 ± 390 ml . min-1 , 50.9 ± 5.2 ml . kg-1 . min-1 , participated in this experiment. The subjects performed an incremental exercise test until exhaustion. The exercise test started at power output of 30 W, followed by an increase amounting to 30 W every 3 minutes. The pedalling rate was maintained at 60 rev . min-1 . Gas exchange variables were measured continuously using breath-by- breath system Oxycon Jaeger. At the end of each step blood samples were taken for lactate concentration. Muscle biopsy samples taken from the vastus lateralis m. quadricipitis femoris, using the Bergstrom needle, were analysed for the content of different MyHC (I, IIa, IIx) using SDS-PAGE and Western blotting. The pre-exercise VO2, as a mean value of six-minute measurements, expressed both in ml . min-1 , and in ml . kg -1 . min-1 , was positively correlated with the content of MyHC II in the vastus lateralis (p < 0.01). We have also found that the pre-exercise values of VO2 in the group of subjects with a high proportion of MyHC II (59.9 ± 11.2 %) were significantly higher (p < 0.02, when VO2 was expressed in ml . min-1 , and p < 0.01 when VO2 was expressed in ml . kg-1 . min-1 ) than in the group with low content of MyHC II (27.5 ± 6.0 %) in the vastus lateralis. Moreover, we have found a significant negative correlation (r = -0.562, p < 0.01) between the slope in the VO2/PO relationship below the lactate threshold (LT) and the content of MyHC IIa in the vastus lateralis. The most interesting finding of our study was that the magnitude of the non-linear increase in the VO2 / power output relationship present above the LT was positively correlated ( r = 0.510, p < 0.02) with the content of MyHC II in the vastus lateralis. Our results show, that there is no simple relationship between the content of different types of MyHC in the vastus lateralis and the oxygen cost of work during incremental exercise test. Individuals with a high content of MyHC II in the vastus lateralis m. quadricipitis femoris consume more oxygen in the pre-exercise conditions than subjects with a low content of MyHC II in their muscles. Subjects with a high content of MyHC II require a smaller increase in VO2 for maintaining a linear increase in power output up to the lactate threshold (lower slope in this relationship), but after exceeding the LT, they consume more oxygen above that expected from the linear relationship below the LT, than the subjects with a low content of MyHC II in their muscles. Therefore, non-linear increase in the VO2 / power output relationship, present above the LT, is more pronounced in subjects with a higher content of MyHC II in the vastus lateralis m. quadricipitis femoris.
Strenuous exercise was reported to involve the alteration in the release of some "stress" hormones such as growth hormone (GH), cortisol, catecholamines and appropriate adjustment of energy metabolism but the relative contribution of these hormones to metabolic response, to cycling exercise performed at different muscle shortening velocities, has not been clarified. Aims: The purpose of this experiment was to assess the effect of applying different pedalling rates during a prolonged incremental cycling exercise test on the changes in the plasma levels of growth hormone, cortisol, insulin, glucagon and leptin in humans. Material and Methods: Fifteen healthy non-smoking men (means ± SD: age 22.9 ± 2.4 years; body mass 71.9 ± 8.2 kg; height 178 ± 6 cm; with VO2max of 3.896 ± 0.544 l . min-1), assessed in laboratory tests, were subjects in this study. The subjects performed in two different days a prolonged incremental exercise tests at two different pedalling rates, one of them at 60 and another at 120 rev . min-1. During this tests the power output has increased by 30 W every 6 minutes. The tests were stopped when the subject reached about 70 % of the VO2max. Results and conclusions: We have found that choosing slow or fast pedalling rates (60 or 120 rev . min-1), while generating the same external mechanical power output, had no effect on the pattern of changes in plasma cortisol, insulin, glucagon, glucose and leptin concentrations. But, generation of the same external mechanical power output at 120 rev . min-1 causes more stepper increase (p < 0.01) in the plasma growth hormone concentration [GH]pl and plasma lactate concentrations [La]pl when compared to that observed during cycling at 60 rev . min-1. We have also found that the onset of a significant increase in [GH]pl during cycling at 60 rev . min-1 was not accompanied by significant increase in [La]pl. While during cycling at 120 rev . min-1 the onset of a significant increase in [La]pl occurred without increase in [GH]pl, but with continuation of exercise when plasma [La]pl increased, there was also a parallel rise in plasma [GH]pl, as reported before. This results indicates that the increase in [GH]pl during exercise is not closely related to the increase in [La]pl.
Background: Although hormonal responses to exercise performed in fed state are well documented, far less in known about the effect of a single exercise bout, performed after overnight fasting, on cardio-respiratory responses and hormones secretion. It has been reported that recently discovered hormones as leptin and ghrelin may affect cardiovascular responses at rest. However, their effect on the cardiovascular responses to exercise is unknown. Aims: This study was designed to determine the effect of overnight fasting on cardio- respiratory responses during moderate incremental exercise. We have hypothesised that fasting / exercise induced changes in plasma leptin / ghrelin concentrations may influence cardiovascular response. Material and Methods: Eight healthy non-smoking men (means ± SE.: age 23.0 ± 0.5 years; body mass 71.9 ± 1.5 kg; height 179.1 ± 0.8 cm; BMI 22.42 ± 0.49 kg . m-2 with VO2max of 3.71 ± 0.10 l . min-1) volunteered for this study. The subjects performed twice an incremental exercise test, with the increase of power output by 30 W every 3 minutes. Tests were performed in a random order: once in the feed state - cycling until exhaustion and second, about one week later, after overnight fasting - cycling until reaching 150 W. Results: In the present study we have compared the results obtained during incremental exercise performed only up to 150 W (59 ± 2 % of VO2max) both in fed and fasted state. Heart rate measured during exercise at each power output, performed in fasted state was by about 10 bt . min-1 (p = 0.02) lower then in fed subjects. Respiratory quotient and plasma lactate concentration in fasted state were also significantly (p<0.001) lower than in the fed state. Pre-exercise plasma leptin and ghrelin concentrations were not significantly different in fed and fasted state. Exercise induced increase in hGH was not accompanied by a significant changes in the studied gut hormones such as ghrelin, leptin, and insulin, except for plasma gastrin concentration, which was significantly (p = 0.008) lower in fasting subjects at the power output of 150 W. Plasma [IL-6] at rest before exercise performed in fasted state was significantly (p = 0.03) elevated in relation to the fed state. This was accompanied by significantly higher (p = 0.047) plasma noradrenaline concentration. Plasma IL-6 concentration at rest in fed subjects was negatively correlated with plasma ghrelin concentration (r = - 0.73, p < 0.05) and positively correlated with plasma insulin concentration (r = 0.78, p < 0.05). Significant negative correlation (r = - 0.90; p < 0.05) was found between plasma insulin and ghrelin concentration at rest in fed subjects. Conclusions: We have concluded that plasma leptin and ghrelin concentrations have no significant effect on the fasting-induced attenuation of heart rate during exercise. We have postulated that this effect is caused by increased plasma norepinephrine concentration, leading to the increase in systemic vascular resistance and baroreceptor mediated vagal stimulation. Moreover we believe, that the fasting-induced significant increase in plasma IL-6 concentration at rest, accompanied by higher plasma norepinephrine concentration and lower RQ, belongs to the physiological responses, maintaining energy homeostasis in the fasting state.
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