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1

Udzial termoregulacji behawioralnej w utrzymaniu homeostazy organizmu oraz rola pozapodwzgorzowej kortykoliberyny i cytokin w modyfikowaniu zachowania sie zwierzat w stanie stresu

100%
Swiergiel A.
Prace i Materiały Zootechniczne. Zeszyt Specjalny
|
2001
|
nr 11
1-64
2

Effects of cannabidiol administration on body weight gains in rats

51%
Ignatowska-Jankowska B., Jankowski M., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Cannabidiol (CBD), the major non-psychoactive compound of Cannabis has been reported to display neuroprotective, anti-infl ammatory, and anti-tumor actions. Despite growing interest in pharmacological effects of CBD, there is no data concerned with the effects of CBD on body weight, food intake or metabolism. The present study examined whether CBD administration affects body weight, during 13 days of systemic CBD treatment. Adult male Wistar rats (n=54), weighing approximately 250 ± 20 g at the beginning of the experiment received intraperitoneal injections of CBD at dose of 2.5 or 5 mg/kg/day, or the vehicle, for 13 consecutive days. The animals were weighed each day 2 h before each injection. Body weight on the fi rst day of treatment served as baseline (100%), then in following 13 days percentage body weight gains were calculated. Repeated CBD administration signifi cantly slowed body weight gains. Two-way ANOVA revealed signifi cant difference between weight gains of rats receiving CBD and the control group (P<0.0001).Rats receiving CBD at dose of 5 mg/kg gained, on average, 8.0 ± 0.9 g (SEM) less weight than control animals, which is 19.6% of total weight gain of controls during that period. Mean body weight gain of rats receiving 2.5 mg/kg of CBD was 6.8 ± 0.9 g lower than controls (16.6% of total weight gain of controls).The results indicate that CBD administered in low doses lowers body weight gain. The data suggest that CBD may affect food intake or metabolism
3

Intravenous cocaine alters limphocyte subsets distribution in peripheral blood of rats

51%
Jankowski M., Ignatowska-Jankowska B., Glac W., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Cocaine use has been associated with the increased risk for HIV and HCV infection in humans. The addicts have faster progression of HIV infection and increased incidence of AIDS suggesting that cocaine use results in a specifi c functional impairment of the immune system. Present study aimed to evaluate lymphocyte subsets number and distribution in peripheral blood after acute series of intravenous cocaine infusions. Adult male Wistar rats received 3 injections of cocaine hydrochloride (5 mg/kg/ml) in 30 min intervals. Blood samples were collected 30 min after the last infusion. Total leukocyte number, percentage numbers of leukocyte subpopulations and T, B, NK, T helper and T cytotoxic lymphocyte subsets were assessed. Intravenous cocaine caused signifi - cant decrease in total leukocyte and lymphocyte number and signifi cant fall in total numbers of T, B, NK and both T helper and T cytotoxic lymphocyte subsets. Furthermore, intravenous cocaine altered proportions between lymphocyte subsets by increasing T, mainly T helper and decreasing B, T cytotoxic and NK lymphocyte percentage portion. Immunosuppressive effect of intravenous cocaine infusions may thus be connected with alterations in lymphocyte subsets proportions. Such destabilization of lymphocyte subsets balance, especially T helper lymphocyte, could constitute basis of increased susceptibility for HIV and HCV infections.
4

Effects of repeated administration of cannabidiol on body weight gain, food intake and accumulation of adipose tissue in rats

45%
Ignatowska-Jankowska B., Jankowski M., Torczynska A., Kurrek A., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Cannabidiol (CBD) is a major non-psychotropic compound derived from Cannabis that has wide therapeutic potential. In our previous studies we observed decreased body weight gain in rapidly growing rats (10 weeks of age at the start of study) treated with CBD for 14 consecutive days. The present study aimed to evaluate the effects of repeated CBD administration on body weight gain, food intake and accumulation of abdominal adipose tissue in rats fed standard (SD) and high fat (HFD) diet. Adult male Wistar rats (n = 36) weighing approximately 400 g at the beginning of the experiment (14 weeks of age), fed with SD only, or having access to free choice HFD (60% kcal from fat, 10% kcal from sucrose) received intraperitoneal injections of CBD or vehicle for 14 consecutive days (5 mg/kg/day). Body weight gain as well as food and water intake were measured daily. Total amount of intra-abdominal adipose tissue was assessed by a dissection method. In contrast to previous observations in younger rats, repeated CBD administration induced significant increase in body weight gain of rats fed SD, but no significant change in food or water intake was observed. CBD produced tendency for decreased body weight gain in rats fed with HFD, but it did not reach level of significance. CBD did not affect total energy intake in rats that had access to HFD, but slightly increased preference for SD. CBD administration reduced intra-abdominal adipose tissue accumulation in rats with access to HFD, but not in rats fed with SD only. The results suggest that CBD may produce different effects on body weight gain depending on age or the metabolic state of the animal. Moreover, the results indicate that CBD may decrease accumulation of intra-abdominal adipose tissue in animals under HFD.
5

Repeated administration of cannabidiol decreases splenic lymphocyte number via CB2 receptors

45%
Ignatowska-Jankowska B., Jankowski M., Torczynska A., Glac W., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Cannabidiol (CBD) is a major non-psychotropic compound of cannabis that has been reported to exert neuroprotective, antipsychotic and anxiolytic effects. CBD has promising anti-inflammatory properities, but despite therapeutic potential its mechanisms of action remain poorly understood. Our previous study revealed decrease in number of lymphocytes B and T in peripheral blood following repeated CBD administration. The present study aimed to assess effects of repeated administration of CBD on distribution of lymphocyte subsets in the spleen and the involvement of CB2 receptors. Adult male Wistar rats (n=35, 10 weeks of age at the start of study) received intraperitoneal injections of CBD at a dose of 5 mg/kg/day, or the vehicle, for 14 consecutive days. Total and relative numbers of lymphocyte T (T CD4+, and T CD8+), B, NK subsets were determined by flow cytometry. The selective CB2 receptor antagonist AM630 (1 mg/kg) was administered 15 min before CBD (or the vehicle) in order to block CB2 receptors. Repeated administration of CBD decreased total leukocyte number resulting from decreased numbers of lymphocytes B and T (both T CD8+ and T CD4+) in the spleen. Pretreatment with CB2 receptor antagonist partially inhibited CBD-induced decrease in lymphocyte number that was most pronounced in case of T CD8+ lymphocytes. AM630 itself produced slight decline in lymphocyte number that did not reach statistical significance. Observed effects were accompanied by a decrease in body weight gain, which was prevented by pretreatment with CB2 antagonist. The results indicate that CBD reduces lymphocyte number in the spleen, as it does in peripheral blood and that CBD has ability to affect the lymphocyte number via CB2 receptor.
6

Effects of acute administration of cannabidiol on food intake in deprived rats

45%
Ignatowska-Jankowska B., Jankowski M., Torczynska A., Kurrek A., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Cannabidiol (CBD) is a major non-psychotropic constituent of Cannabis. The endocannabinoid system, which can be modulated by administration of CBD, is known to play an important role in control of food intake and energy balance. In recent years, numerous studies have revealed various pharmacological effects of CBD such as neuroprotective, analgetic, anxiolytic, antipsychotic and anti-inflammatory properties, and also antitumor potential. Surprisingly, very few reports concern effects of CBD on feeding behavior. Our previous studies revealed decreased body weight gain following repeated CBD administration in rats. The present study aimed to assess acute food intake in deprived rats under standard (SD) and high fat diet (HFD). Adult male Wistar rats (n=40), weighing approximately 350 ± 30 g were food deprived for 24 h and 30 minutes before return of food rats received intraperitoneal injections of CBD (5 mg/kg) or the vehicle. Rats were fed with SD (12% kcal from fat) only, or had additional access to HFD (60% kcal from fat, 10% kcal from sucrose). Food and water intake was measured 1 h, 2 h, 3 h and 24 h after animals were allowed ad libitum access to their assigned diet. Administration of CBD induced significant decrease in food intake of deprived rats within first 3 hours after food was returned in rats fed with SD only. Total energy intake within 24 h was not significantly altered in rats fed with SD. No change in total energy intake or preference of SD and HFD was observed in rats that had access to free choice HFD. CBD did not affect water intake or defecation. The results indicate that acute dose of CBD may decrease food intake in food deprived animals fed with SD, but not in rats having access to HFD. The data indicate that effects of acute CBD administration in deprived rats depend on type of diet.
7

Effect of advanced paternal age on ultrasound vocalization in mice

45%
Zacchini F., Sampino S., Swiergiel A., Loi P., Ptak G.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
8

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

39%
Sampino S., Zacchini F., Swiergiel A., Modlinski J., Loi P., Ptak G.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Prenatal developmental period is a critical window, where any perturbation of embryonic/fetal environment can lead to behavioural abnormalities and diseases in adult life, such as depression and stress disorders. The mechanisms underlying long term effects, induced by early perturbation, are not well described, but an epigenetic origin has been suggested. In this work we wanted to investigate whether and how blastomere biopsy on 8-cells stage embryos can have long-term effects on behaviour. Onemonth-old mice derived from the biopsed embryos were subjected to a battery of behavioural tests. The animals displayed an increased locomotor and exploration activity (p<0.05) and increased anxiety-like behaviour. Interestingly, the depressionlike behaviour in the tail suspension test was observed only in female offspring (p<0.001). In addition, to investigate the epigenetic mechanism underlying these behavioural alterations, we analyzed expression of imprinted genes Snrpn, Peg1 and Ube3a in blastocysts obtained after biopsy. These imprinted genes are highly expressed in pre-implantation embryos, where their epigenetic programming is defined, and in brain, shaping the behavioural phenotype of offspring. Real-Time PCR analysis revealed significant down-regulation of Peg1 (p<0.05), Snrpn and Ube3a (p<0.01) in blastocysts derived from the biopsed embryos, compared to controls. The results suggest that blastomere biopsy causes an altered expression of imprinted genes in preimplantation embryo. The reduction of expression of these transcripts can cause anxiety- or depression-like behaviours and alteration of locomotory activity in offspring obtained following biopsy of early embryos.
9

Effects of cocaine on the distribution on lymphocyte subsets in the speen of rats: possible involvement of dopamine receptors

39%
Jankowski M., Ignatowska-Jankowska B., Glac W., Torczynska A., Kurrek A., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
10

Effect of advanced paternal age on ultrasound vocalization in mice

39%
Zacchini F., Sampino S., Swiergiel A., Modlinski J., Loi P., Ptak G.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Delayed parenthood is a growing phenomenon in western countries due to modern lifestyle. In particular, delayed fatherhood has been described as a factor of risk for several mental disorders and intermediate phenotypes such as poor cognitive or social functions. In this work we want to investigate whether and how advanced paternal age can affect development of offspring. We evaluated UltraSound Vocalization (USV) activity on 4-day old mice, born from both aged and young (control) father, after removal from their nest. We found that the total number of USV in the 5 minutes of testing was significantly higher in pups from aged mice than control (p<0.002) and that the main difference was present during the first minute (p <0.0004). Also, our data showed that the number of high intensity USV was increased in pups from aged mice (p<0.002). We also evaluated the righting reflex ability of 6 and 10-days-old mice. We did not find any difference in righting reflex ability between groups. All together our data show that delayed fatherhood affect communication skills and anxiety likebehavior but no innate righting reflex ability. In conclusion, advanced paternal age affects the development of offspring and these effects are detectable already in first days of post-natal life and they could be first signs of brain diseases in adult life. Future analysis will be necessary to define the long term effect of advanced paternal age and the mechanism underlying developmental alterations in offspring.
11

Effects of cocaine on the distribution of lymphocyte subsets in the spleen of rats: possible involvement of dopamine receptors

39%
Jankowski M., Ignatowska-Jankowska B., Glac W., Torczynska A., Kurrek A., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
The expression of dopamine, dopamine receptors and dopamine active transporters in peripheral lymphoid tissues and lymphocytes suggest that increased dopaminergic activity induced by cocaine may be involved in regulation of the distribution of lymphocyte subsets in the spleen. Adult male Wistar rats implanted with jugular vein catheter received cocaine hydrochloride (3 × 5 mg/kg/ml, i.v., at 30 min intervals) or saline infusions. Animals were pretreated with haloperidol (1 mg/kg/ml, i.v.) or vehicle 10 min prior to the first cocaine infusion. After each cocaine infusion the locomotor-activating effects of cocaine were measured. The spleens were collected 30 min after the third cocaine infusion and total splenocyte numbers and percentage numbers of leukocyte subpopulations were assessed using a morphological method. Three-color immunofluorescent antibody staining procedure (CD3-FITC/CD45RA-PC7/CD161A-APC and CD3-FITC/ CD4-PC7/CD8-APC) was used for determination of T, B, NK, T CD4+ and T CD8+ lymphocyte subsets. Plasma corticosterone and serum concentrations of IL-4, IFN-gamma and cocaine were assessed. In the spleen, administration of cocaine after pretreatment with haloperidol decreased numbers of splenocytes, lymphocytes and T CD4+ and B lymphocytes, significantly in comparison to rats treated with cocaine alone. The proportions between lymphocyte subsets and CD4/CD8 ratio in the spleen were not affected. Cocaine or/and haloperidol increased plasma corticosterone concentration. Serum cocaine concentration indicated the possibility of accumulation of cocaine in the applied schedule of administration. Serum concentrations of IL-4 and IFN-gamma were not altered. Haloperidol abolished locomotor response induced by cocaine infusions. In conclusion, dopaminergic activity following administration of cocaine is involved in retention of T CD4+ and B lymphocytes in the spleen of rats.
12

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

39%
Sampino S., Zacchini F., Swiergiel A., Modlinski J., Loi P., Ptak G.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Prenatal developmental period is a critical window, where any perturbation of embryonic/fetal environment can lead to behavioural abnormalities and diseases in adult life, such as depression and stress disorders. The mechanisms underlying long term effects, induced by early perturbation, are not well described, but an epigenetic origin has been suggested. In this work we wanted to investigate whether and how blastomere biopsy on 8-cells stage embryos can have long-term effects on behaviour. One-month-old mice derived from the biopsed embryos were subjected to a battery of behavioural tests. The animals displayed an increased locomotor and exploration activity (p<0.05) and increased anxiety-like behaviour. Interestingly, the depression-like behaviour in the tail suspension test was observed only in female offspring (p<0.001). In addition, to investigate the epigenetic mechanism underlying these behavioural alterations, we analyzed expression of imprinted genes Snrpn, Peg1 and Ube3a in blastocysts obtained after biopsy. These imprinted genes are highly expressed in preimplantation embryos, where their epigenetic programming is defined, and in brain, shaping the behavioural phenotype of offspring. Real-Time PCR analysis revealed significant down-regulation of Peg1 (p<0.05), Snrpn and Ube3a (p<0.01) in blastocysts derived from the biopsed embryos, compared to controls. The results suggest that blastomere biopsy causes an altered expression of imprinted genes in preimplantation embryo. The reduction of expression of these transcripts can cause anxiety- or depression-like behaviours and alteration of locomotory activity in offspring obtained following biopsy of early embryos.
13

Subdiaphragmatic vagotomy does not protect against the increase of noradrenergic response of the rat

39%
Wieczorek M., Kobrzycka A., Hubner M., Oclon E., Zubel J., Swiergiel A.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Peripheral administration of gram-negative bacteria endotoxin – lipopolisaccharide (LPS) is known to activate the hypothalamo-pituitary adrenal axis (HPAA) and brain noradrenergic systems. We studied the vagotomized rats responses to peripherally administered LPS using the HPLC-ED to measure the concentration of noradrenaline and their metabolite MHPG in various brain regions. METHODS: Rats were submitted to subdiaphragmatic vagotomy and after 30 days we used them for experiments. They were injected with saline (100 μl ip) and LPS (10 μg/100 μl ip) in random order, and two hours after injection they were euthanized. They brains where removed from the skull and we isolated the hypothalamus, amygdala, prefrontal medial cortex, hippocampus periaqueductal gray matter and the brainstem and submitted for chromatographic analysis. RESULTS: Future chromatographic analysis indicates, that subdiaphragmatic vagotomy did not protect against increase of noradrenaline concentration in analyzed brain regions. In case of LPS injected animals we observed increased noradrenaline concentration versus saline injected ones. These results were comparable with those observed in sham operated rats. CONCLUSIONS: There results suggest that there may be compensatory mechanisms, responsible for transferring of immune signal to the brain, and develop during such a long time of recovery after subdiaphragmatic vagotomy. Supported by NCN, grant no. UMO-2012/07/B/NZ4/00205
14

Divergent selection of mice for high and low swim stress-induced analgesia alters polymorphism at microsatellite loci

33%
Fedorowicz K., Sacharczuk M., Konarzewski M., Lapo I., Kawka M., Sadowski B., Swiergiel A., Jaszczak K.
Animal Science Papers and Reports
|
2007
|
tom 25
|
nr 4
15

Effect of acute and repeated social stress on hippocampal transcriptome in mice

33%
Stankiewicz A., Zabielska J., Milewczyk A., Korowin M., Gasiorkiewicz I., Swiergiel A., Juszczak G., Lisowski P.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
The aim of the study was to investigate dynamics of transcriptomic changes in hippocampus during stress in correlation with behavioral and physiological symptoms of acute and chronic stress response. Male Swiss Webster mice were subjected to repeated social stress and decapitated after 1, 4, 8, and 13 days of repeated encounters with other male mice. There was also a group of mice subjected to 13 days of social stress and then left without stress for 5 days. Acute stress induced decrease in food intake and in body weight. Repeated stress induced thymic involution progressing with increasing duration of stress and significant increase in spleen weight, was observed after 13 days of stress. In mice subjected to stress and then left for 5 days for recovery the spleen weight did not differed from control mice and there was partial recovery of thymic weight. During the recovery period there was also increased food intake compared with control mice. Using microarray and quantitative real-time PCR technologies we found that social stress affected hippocampal transcription of genes involved in pathways of insulin secretion, intracellular signaling and cellular transport. Among them, during subsequent time points of social stress we observed progressive upregulation of Ttr gene coding transthyretin involved in amyloidosis, seizures, or dementia and prolactin receptor – Prlr, involved in anxiolyting effects at brain level. The results show that repeated stress provokes major changes in hippocampal physiological pathways. Effects of stress on expression of genes involved in insulin signalling and cellular transport indicate that stress may affect the CNS structure and produce the long-term and irreversible changes in the CNS functions.
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