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The purpose of the study was to define if anaesthetic action of xylazine could conceivably result from the potentiation of inhibitory neurotransmitters or the inhibition of excitatory neurotransmitter systems in the brain. Rats were injected with xylazine at a dose of 50 mg/kg b.w., and then the hippocampus and thalamencephal were removed at 0.1, 0.25, 0.5, 1, 1.5, 2, 4, and 6 h after the injection. Glutamate (Glu) and γ-aminobutyric-acid (GABA) were measured in the brain tissue by reversed-phase high-performance liquid chromatography. The results revealed that the hippocampus Glu level decreased significantly 0.1 h after the injection of xylazine, the thalamencephal GABA increased significantly 0.1 h after the injection, while the changes in hippocampus GABA and thalamencephal Glu were not significant. However, all of these changes returned to the normal level after 2 and 4 h, respectively. The results indicated the relative effects of xylazine on Glu and GABA levels in the hippocampus and thalamencephal.
This paper reports on a hydroponics experiment that was conducted to investigate the effect of inorganic arsenics on the seedlings of the rice cultivar Shanyou63. The seedlings were subjected to two treatments, i.e., As(III) and As(V). The results showed that the morphological traits of the seedlings were significantly altered after the arsenic treatments. Analysis of nitrogen, phosphorus, potassium, and arsenic contents of the roots and leaves of the seedlings indicated that the absorption of phosphorus and potassium was mainly affected by As(III), while that of nitrogen was mainly affected by As(V). The expression of 12 genes involved in the absorption and utilization of nitrogen, phosphorus, and potassium were all observed to be down-regulated after the arsenic treatments. As(V) significantly affected the absorption and utilization of nitrogen, while As(III) significantly affected those of phosphorus and potassium. The result obtained by realtime FQ-PCR regarding the difference in the gene expressions agreed with that of our hydroponics experiment.
Alimentary oligofructose (OF) overload induces acute laminitis in dairy heifers. We examined the correlation between acute bovine laminitis and antioxidant levels by measuring the activities of malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD) in dairy heifers with acute bovine laminitis. A total of 12 clinically healthy non-pregnant Chinese Holstein heifers, aged between 18-26 months (20.67 ± 3.01 mo), weighing 335-403 kg (379.71 ± 19.87 kg), and with BCS ranging from 2.7 to 3.3 were selected and divided into two groups of six animals: an OF-treated group and a control group. The OF-treated heifers (n = 6) received 17 g/kg BW of oligofructose dissolved in 2 L/100 kg BW of tap water, whereas the control heifers (n = 6) received 2 L/100 kg BW of tap water. Blood samples of OF-treated and the control heifers were collected at –72 h before, as well as 0, 6, 12, 18, 24, 36, 48, 60 and 72 h after OF overload. Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) activity levels were measured by authenticated standard methods using blood samples. The mean concentration of superoxide dismutase (SOD) was significantly lower (p < 0.01) in the OF-treated heifers from 24 h to 72 h and significantly lower (p < 0.05) at 18 h compared to the control group. Malondialdehyde (MDA) level was significantly higher (p < 0.01) at 24 h to 72 h and significant increased (p < 0.05) at 12 h to 18 h in OF-treated heifers compared with healthy heifers. There were no significant differences in GSH and CAT activities between the control and treatment groups. Our study showed that inadequate levels of antioxidants (enzymatic and non-enzymatic) may be linked to oxidative stress in sick heifers. OF-treated heifers had decreased SOD and increased MDA in response to acute laminitis. Therefore, SOD, GSH, CAT, and MDA activities may play a dynamic role in the etiopathogenesis of acute laminitis in dairy heifers. However, further detailed research is required to describe these responses and to develop strategies to control acute laminitis.
The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R² = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.
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