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1

Meat products as functional foods. A paradox?

100%
Pegg R. B.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
2
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Content available

Content of tocopherol isomers in oilseed radish cultivars - a short report

63%
Pegg R. B., Amarowicz R.
Polish Journal of Food and Nutrition Sciences
|
2009
|
tom 59
|
nr 2
Three oilseed radish (Raphanus sativus [cv. Daikon, China Rose, and German]) cultivars from two research farms in Saskatchewan, Canada, were analysed for their tocopherol contents. The findings were compared to tocopherol levels determined in an oriental mustard, a field mustard, a canola, and an arugula cultivar. HPLC data revealed that the oilseed radish cultivars were devoid of both α- and β-tocopherol, but contained γ-tocopherol at levels similarly found in many canola varieties (i.e., 11.4 mg/100 g seed). In addition, they possessed a small content of δ-tocopherol (~1-1.4 g/100 g seed). Application of a YMC™ Carotenoid (C30) column successfully separated and allowed quantification of all tocopherol isomers using reversed‑phase HPLC with UV detection in <15 min.
3

Content of gallic acid in selected plant extracts

51%
Karamac M., Kosinska A., Pegg R. B.
Polish Journal of Food and Nutrition Sciences
|
2006
|
tom 15/56
|
nr 1
Ethanolic extracts of phenolic compounds were prepared from green teas (Camellia sinensis L.), bearberry leaves (Arctostaphylos uva-ursi L.), hazelnuts (Corylus avellana), evening primrose (Oenothera biennis) and grape seeds (Vitis vinifera L.). All crude extracts were examined for their gallic acid content by HPLC. The bearberry-leaf preparation was also fractionated by a Sephadex LH-20 column chromatographic method with 95% (v/v) ethanol and acetone:water (1:1; v/v) as mobile phases: one fraction consisted of low molecular-weight phenolics and the other of tannins. To all samples tannase was applied in an effort to liberate gallic acid from the phenolic esters and hydrolysable tannins. The content of free gallic acid in the extracts ranged from 1 to 15 mg/g with the highest quantity being found in evening primrose. The content of gallic acid liberated by tannase ranged from 5 to 309 mg/g with the highest content being found in the tannin fraction from the bearberry-leaf extract.
4

Confirming the chemical structure of antioxidative trihydroxyflavones from Scutellaria baicalensis using modern spectroscopic methods

51%
Pegg R. B., Amarowicz R., Oszmianski J.
Polish Journal of Food and Nutrition Sciences
|
2005
|
tom 14/55
|
nr 1
5

Metody peklowania bezazotynowego. Zastapienie tradycyjnego procesu

51%
Pegg R. B., Fisch K. M., Shahidi F.
Mięso i Wędliny
|
2001
|
nr 7
46
6
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Determination of hippuric acid by RP-HPLC using two different analytical columns - a short report

51%
Pegg R. B., Rybarczyk A., Amarowicz R.
Polish Journal of Food and Nutrition Sciences
|
2007
|
tom 57
|
nr 4
447-450
Reversed-phase HPLC of liberated hippuric acid (HA) from an ACE assay in the presence of ACE inhibitory peptides derived from a crackling hydrolysate was conducted. The efficacies of two different analytical HPLC columns using identical mobile phases with an isocratic system were tested. Chromatograms revealed that the shorter C8 column (4.6 × 150 mm, 5 μm) was just as efficient as the longer C18 column (4.6 × 250 mm, 5 μm) in resolving liberated HA, but achieved this in a much shorter time (i.e., 3.67 cf 12.52 min). The presence of the crackling hydrolysate exhibited ACE-inhibiting activity by retarding the liberation of HA from the substrate hippuryl-L-histydyl-L-leucine (HHL) in a dose-dependent manner, and did not interfere with the chromatography. Hence, a quick reliable analytical methodology is at hand for the in vitro examination of various “bioactive peptide concoctions” for possible use in the development of functional foods.
7
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Chromatographic separation of tannin fractions from a bearberry-leaf (Arctostaphylos uva-ursi L. Sprengel) extract by SE-HPLC - a short report

51%
Pegg R. B., Rybarczyk A., Amarowicz R.
Polish Journal of Food and Nutrition Sciences
|
2008
|
tom 58
|
nr 4
Phenolic compounds from the leaves of the bearberry plant (Arctostaphylos uva-ursi L. Sprengel) were extracted into 95% (v/v) ethanol. Tannin fractions were obtained by Sephadex LH-20 column chromatography using 50% (v/v) acetone as the mobile phase. The tannin fraction was then further characterised by size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SWXL column with a mobile phase comprising 45% acetonitrile and 0.1% (v/v) TFA. The chromatography revealed the presence of additional phenolic compounds in the tannin fractions which are not proanthocyanidins (i.e. condensed tannins).
8

Comparison of radical-scavenging activities for selected phenolic acids

51%
Karamac M., Kosinska A., Pegg R. B.
Polish Journal of Food and Nutrition Sciences
|
2005
|
tom 14/55
|
nr 2
9
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Improved method of wheat starch isolation for friabilin analysis

51%
Budny J. A., Fornal J., Amarowicz R., Pegg R. B.
International Agrophysics
|
2005
|
tom 19
|
nr 2
10
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Content available

Capillary zone electrophoresis [CZE] of flaxseed phenolic constituents - a short report

51%
Rybarczyk A., Pegg R. B., Amarowicz R.
Polish Journal of Food and Nutrition Sciences
|
2008
|
tom 58
|
nr 2
Phenolic compounds were extracted from flaxseed using 80% (v/v) methanol. The resultant crude extract was hydrolysed under basic conditions. Secoisolariciresinol diglucoside (SDG) was separated from the prepared extract using semi-preparative HPLC. Secoisolariciresinol (SECO) was liberated from SDG by acid hydrolysis. The chemical structures of SDG and SECO were confirmed by ESI-MS. Capillary zone electrophoresis (CZE) was employed for the separation of phenolics from the crude extract, the extract resulting after base hydrolysis, and the two lignans. CZE was found to offer a quicker analysis of SDG with higher separation efficiency than with a conventional HPLC methodology. The retention time for SDG was noted at 5.33 min. The migration times of SDG and SECO were close together; a base line separation of both lignans was not achieved. The electropherogram of the crude flaxseed extract showed only one broad peak with a retention time of 6.02 min.
11
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Chromatographic separation of phenolic compounds from rapeseed by SE-HPLC - a short report

51%
Karamac M., Kosinska A., Rybarczyk A., Pegg R. B.
Polish Journal of Food and Nutrition Sciences
|
2007
|
tom 57
|
nr 1
Phenolic compounds from rapeseed were extracted into 80% (v/v) methanol. The crude extract was then separated using size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SWXL column with a mobile phase of 45% acetonitrile and 0.1% TFA (v/v). Six peaks were identified on the chromatogram: the main peak originated from sinapin. Calibration of the assay was characterised by an excellent linearity of the sinapin standard (r2=1.0). The method so described can be used for screening phenolic constituents in rapeseed or canola. It is also useful for the purification of the sinapin as a standard from the crude extract.
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