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Muszka - najlepszy przyjaciel neurobiologa

100%
Damulewicz M.
Wszechświat
|
2010
|
tom 111
|
nr 04-06
92-96
2

Komorki macierzyste - terapia XXI wieku

100%
Damulewicz M.
Wszechświat
|
2008
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tom 109
|
nr 07-09
214-217
3

Localization of cryptochrome-immunopositive neurons in the visual system of Drosophila melanogaster

100%
Damulewicz M.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Cryptochrome (CRY) is a blue light absorbing protein involved in the photic entrainment of circadian clock in the fruit fl y. It has also been suggested that CRY may play role in molecular circadian clock that generates circadian rhythms in the visual system. The aim of our study was to examine localization of CRY-immunopositive neurons in the visual system of Drosophila and changes in abundance of CRY protein in these neurons. We used Drosophila transgenic lines which express green fl uorescent protein (GFP) under control of the cry promotor. Analyses of GFP fl uorescence showed that CRY is present in the dorsal neurons DN3, and in the dorsal (LNd) and ventral lateral neurons (LNv) and its density decreases during the day and increases during the night. In the LNv and in the processes of DN3, the CRY abundance was the highest at ZT1 (1 h after lights-on) and the lowest at ZT13 (1 h after lights-off) in both males and females. In addition we found that the LNvs project to the second visual neuropil (medulla) and form CRY-immunopositive network of many varicose processes in the medulla. We also detected numerous CRY-immunopositive processes in the fi rst optic neuropil (lamina) which origin from a single fi bre extending from the LNvs. In the lamina it divides into many thin branches next to the retina. Our results showed for the fi rst time that the lamina is invaded directly by processes of clock neurons which seem to control circadian plasticity of neurons and synapses in the lamina.
4

Neuroplasticity in the visual system of Drosophila melanogaster

51%
Gorska-Andrzejak J., Damulewicz M., Pyza E.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2012
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tom 54
|
nr 1
5

Clock-controlled daily remodeling of neurons and synaptic contacts in the visual system of drosophila

51%
Pyza E., Gorska-Andrzejczak J., Damulewicz M.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
In the first optic neuropil (lamina) of the visual system of Drosophila melanogaster, the first order interneurons receive photic and visual inputs from the overlying retina photoreceptors. Next they filtrate, enhance and transmit this information to the second optic neuropil (medulla). The inputs from the photoreceptors are transmitted by means of tetrad synapses, using histamine as a neurotransmitter, to 2-3 lamina interneurons, glial and amacrine cells. Among the lamina interneurons, L1 and L2 monopolar cells show circadian morphological plasticity. Their axons swell at the beginning of both the day and night and shrink at other times of the day. These changes in neurons are offset by morphological changes of glia in the lamina. The pattern of size changes of L1 and L2 axons is correlated with the pattern of D. melanogaster locomotor activity, which has two peaks, in the morning and in the evening. Moreover, the tetrad synapses in the lamina show similar structural oscillations, however, the rhythm in abundance of a presynaptic protein Bruchpilot (BRP) in the photoreceptor terminals is only maintained in a day/night (LD) regime but not in constant darkness (DD). It means that the density of tetrad presynaptic elements depends on light. In contrast to the presynaptic elements of tetrad synapses, their postsynaptic partners, dendrites of the L2 monopolar cells, change their structure not only in LD but also in DD. They are largest at the beginning of day. It indicates that the number of presynaptic elements of the tetrad synapses is regulated by direct exposure of light while organization of the postsynaptic sites is controlled by a circadian clock. In result circadian morphological plasticity of the L2 dendrites is light independent and driven by a circadian input from the circadian clock located in the brain.
6

Effects of PINK1 mutation on synapses and behavior in the brain of Drosophila melanogaster

45%
Doktor B., Damulewicz M., Krzeptowski W., Bednarczyk B., Pyza E. M.
Acta Neurobiologiae Experimentalis
|
2018
|
tom 78
|
nr 3
Mutations in the PINK1 gene are responsible for typical symptoms of Parkinson’s disease. Using Drosophila melanogaster mutant PINK1B9 and after PINK1 silencing with RNAi using transgenic lines, we observed defects in synapses and behavior. The lack or reduced expression of PINK1 prolonged sleep during the day (nap) and decreased the total locomotor activity during 24 h, in addition to a decrease in climbing ability and a reduced lifespan. In the brain, PINK1 mutants had a lower level of Bruchpilot (BRP), a presynaptic scaffolding protein that is crucial for neurotransmission in all type of synapses in Drosophila. In addition, other proteins that are involved in synaptic transmission; Rab5, Syntaxin and Wishful Thinking were also decreased in abundance in mutants, except Synaptotagmin. Transmission electron microscopy (TEM) also confirmed less and abnormal synaptic vesicles at tetrad synapses in the visual system of PINK1 mutants. The lower level of BRP and longer day sleep observed was also detected in white mutants, which were examined to test the effect of the white background on the PINK1B9 strain. The reduced locomotor activity and longer day sleep in PINK1 mutants and after decreasing the PINK1 level in neurons seem to be correlated with a decrease in mitochondria number during the day, when they normally peak, and with impaired synaptic transmission.
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