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The aim of the study was to evaluate the quality of pasteurized milk found in retail outlets. The milk was derived from seven different dairies. Eight parameters of milk were the basis of the evaluation of its quality. The parameters included the following: a) physical - temperature of the milk and its density at the retail outlets; b) chemical - acidity, dry non-fatty weight content, fat, protein, lactosis and water; c) hygienic - presence of inhibitory substances. The evaluation was conducted in accordance with the method proposed by Polish Standards. Significant differences were confirmed between the dairies in all the evaluated parameters. This attests to the differing quality of the raw milk from which milk for consumption is subsequently produced. The quality of milk was also compared with standards from the directives of the Ministry of Agriculture and Rural Development that were valid until January 19, 2008, with those directives that were binding at the time of these investigations from August 18, 2004 concerning veterinary requirements for milk and milk products, as well as in the Polish Standards for milk for consumption, PN-A-86003: 1996. It was found that the milk quality of a certain portion of milk that is available for sale is not in accord with the binding directives. It is milk of a higher level of water, not rarely overly acidic, of a lower level of dry non-fatty weight and fat content than claimed, or containing an unacceptable level of inhibitory substances. The obtained results indicate insufficient quality control of the produced milk on the part of the dairies, and that the units monitoring milk sales do not sufficiently care about the conditions in which the milk is stored.
The purpose of the work was to establish the susceptibility of pigs to halothane on a farm situated in the Lublin region. Two hundred and thirty five pigs of Large White Polish breed and its cross-breeds were exposed to halothane anaesthesia. A positive reaction expressed by muscle contraction especially of posterior legs was found in two individuals i.e. in 0.8G per cent of animals under study. The results indicated to the usefulness of the test in practice. As the Hal gen did not penetrate completly and there appeared resistance of heterozygotic carriers to halothanc it seemed to be reasonable to includ the studies on polymorphism of proteins (Phi, Pgd, Po2) and blood groups to the breed programme. This kind of procedure should allow to diminish the number of halothane positive animals breed in Poland.
Due to certain properties of ultra violet rays they are primarily used for sterilizing a surface or the air. UV rays are used, among others, to disinfect isolation wards for sterile work, laminar flow cabinets, bacteriological and virusological laboratories, operating rooms, rooms in animal houses, as well as the surfaces of tables, walls or floors. The evaluation of bacterial pollution as well as the effectiveness of methods for sterilizing the air remain relevant problems in many fields, e.g. in industry, animal production, investigative laboratories or health clinics. The aim of the study was to evaluate the degree that the level of bacterial air pollution was reduced in a cabinet for microbiological investigations, sterilized by a UV lamp with a 30 watt florescent lamp. Sampl'air Lite (AES Laboratoire Chemunex) apparatus was used to gather air from the atmosphere. Samples were sampled after 15, 30, 45, 60, 75, 90, 120, 150 and 180 minutes of sterilizing the air in the cabinet with 6 repetitions for each period of irradiation. The initial and final level of the bacterial air pollution in the cabinet for microbiological investigations in relation to the period of the action of the UV rays as well as the level of bacterial reduction were evaluated. The minimal period of the action of the UV rays that ascertained a statistically significant and simultaneously the largest reduction of the bacterial air pollution was 180 minutes. With regards to all the remaining periods of irradiation no essential differences in the levels of bacterial reduction were noted, despite considerable (1.7-4.0 times) reductions of bacteria. An essential correlation was indicated between initial air pollution in the cabinets and 15, 45, and 60 minute periods of the action of UV rays. The correlation coefficients were 0.95 (p ≤ 0.01), 0.82 (p ≤ 0.05) and 0.96 (p ≤ 0.01), respectively. In the remaining cycles of the experiment the correlations were not statistically significant, and the correlation coefficients include in the interval of 0.45-0.60. There is a need, also noted by other authors, of unifying acceptable levels of microbiological air pollution for the purposes of laboratory investigations and production processes.
The objective of the presented study was to determine microbial contamination of calf muscle tissues in relation to slaughtering process of calves during a slaughter day. In order to determine the total aerobic bacteria count, and the presence of bacteria from the Enterobacteriaceae family and enterococci, 32 carcasses were examined (eight each slaughter day), while 40 carcasses (10 each slaughter day) were examined for the detection of Salmonella organisms. Microbiological evaluation of each bacterial group was performed according to the Polish Norms. In most cases, no significant differences were reported between the total aerobic counts on calf carcass surfaces as determined at each slaughter cycle. The daily log mean values were lower by 1 up to 1.5 log, respectively , than the maximal bacteria count (M). Bacteria from the Enterobacteriaceae family were isolated from 59.4% of the evaluated samples. However, contamination with these bacteria was insignificant - 1.7 x 10 cfu/cm². Enterococci were isolated from all investigated samples and contamination ranged from 1.1 x 10 cfu/cm² up to 4.1 x 10² cfu/cm². Salmonella strains were not found in any of the evaluated samples. The order of the slaughtering calves during a slaughter day and the day of the week when the examination was performed did not have any influence on total microbial contamination of carcasses. Thus, sanitary conditions in the examined abattoir were satisfactory and slaughter process was conducted at a high quality level.
Upon Poland’s entering the EU in 2004, dairy factories were modernized and adapted to EU standards. Only raw milk with a content not exceeding 100 thousand bacteria and up to 400 thousand somatic cells per 1 cm3 could be used for the production of drinking milk. In some regions of Poland milk did not meet these standards which made it impossible for dairy producers to maintain continuous production. In these conditions some of the regions gained a transition period, until the end of 2006, during which they had the right to “segregate milk”, that is to collect milk that meets EU standards, as well as milk that does not meet them, i.e. with a higher bacteria count (up to 400 thousand) and somatic cell count (up to 500 thousand per 1 cm3). The aim of the investigation was evaluating the quality of the raw milk collected by dairies from the producers that were in the adaptation period for EU standards representing two different regions in the eastern part of Poland. The investigations were conducted on raw milk collected from individual farms for two dairy factories (A and B) from different regions of the eastern part of the country. The producers were small farms that maintained an average of 3-4 cows. After it was transported to the dairy, the milk temperature was evaluated according to Polish standards, along with its density, acidity, freezing point, total bacteria count, somatic cell count per 1 cm3, as well as the presence of retarding substances. It was stated that in comparison with milk collected by dairy A, milk from dairy B was characterized by higher acidity, a higher bacterial and somatic cell count per 1 cm3 contamination as well as presence of retarding substances. Milk from both dairies did not meet the lower transitional standards and hygienic standards and was unfit for consumption. The results are give in table 1. They indicate the difficulty small farms have in adapting to the necessary hygienic standards. Poland is among the countries with a strongly fragmented milk production, which results in lesser technological advances in small farms than in the large ones. The implementation of sanitary-veterinary standards connected with the conditions of maintaining the cows’ health and handling of the milk after milking is also less advanced. What is crucial in this matter are economic factors as well as ingrained habits of a low hygienic regime in milking and milk storage. However, in the general evaluation of the hygienic quality of Polish milk it is possible to express a measure of satisfaction that a good deal of the measures taken regarding the problem since 2004 have already produced good results.
The PSE (pale, soft, exudative) and DFD (dark, firm, dry) syndromes were determined in 200 pigs and 201 slaughter cattle originated from the Lublin region. There were estimated pH1, pH24 and R index; besides, there were calculated a correlation between the pH1 measurements and R index and the size of carcasses. Qualitative PSE and DFD deviations were found in 133 pigs, i.e. in 66.5 per cent of the animals under study; in 61.5 per cent there were found distinct or partial PSE changes, while in 5 per cent the lesions of the DFD type. The changes of this type were also observed in 42.5 per cent of cattle, in 34 per cent the lesions were of the PSE type and in 8.5 per cent of the DFD type. The highest susceptibility to myopathy concerned m. semitendinosus, then m. semimembranosus and m. longissimus p. lumborum. In cattle myopathy was stated most often in m. psoas major and then in m. longissimus p. lumborum in which the DFD syndrome took place in most cases. There was found a significant correlation between pH1 and the size of carcasses.
The aim of the study was to determine the bacterial contamination of lamb carcasses in relation to the number of slaughtered animals during a day of slaughtering. The investigations were conducted on carcasses derived from a slaughterhouse that had accreditation for market production. In the investigated slaughterhouse the slaughter of the animals is carried out in one cycle, during one day of the week (most frequently on Mondays). The average number of animals slaughtered at the facility comes to 164 lambs. In order to determine the general number of aerobic bacteria, bacteria from the Enterobacteriaceae family and fecal streptococcus, 32 carcasses were examined (8 from each slaughter cycle); in order to determine the presence of Salmonella rods, 50 carcasses were examined (12 in 3 slaughter cycles and 14 in the fourth). Determining specific groups of microorganisms was conducted in accordance with Polish Norms (PN). Samples for examination were collected by the destructive and non-destructive methods (swabbing technique with sterile cotton swab) from the chuck steaks, saddle, brisket and thigh in accordance with PN. Each daily slaughter cycle was divided into 4 phases. In phase I the number of slaughtered animals did not surpass 25% of the average number of animals scheduled for slaughter in a given day, in phase II - the number was within the 51-60% division, in phase II - the number was within 61-74%, and in phase IV it was always at a level above 75%. In every phase samples were gathered from 2 carcasses (n = 8). In order to calculate the logarithmic daily average for the examined slaughter cycles (days) the results gained for the samples gathered in the II, III and IV phases of slaughter (n = 6) were taken into account. The obtained results were statistically analyzed with the average value and standard deviation calculated. The variability factor was determined on the basis of an analysis of the variance through the application of T-Tukey’s multiple confidence intervals for p ≤ 0.05. The investigation confirmed that the order of the slaughter of lambs in the course of the slaughter day does not significantly influence the level of the general number of bacteria on the surface of the examined carcasses. The results demonstrated that the general contamination of the lamb carcasses with aerobic bacteria vacillated from 8.0 × 10² jtk/cm² (phase III) to 2.9 × 10³ jtk/cm² (phase I). Nor were essential differences between specific slaughter phases confirmed within the range of the general number of microorganisms. The contamination was within the division of 7.5 × 10² (2.87 log) to 2.0 × 10³ (3.34 log) jtk/cm². The obtained logarithmic daily averages of contamination did not exceed boundary values for the number of microorganisms (m) determined for the hygiene standard for the sheep slaughter process in the order of the Commission 2073/2005. Bacteria from the Enterobacteriaceae family were confirmed in 18 samples (56.25%), and fecal streptococcus in all the examined samples. No significant differences were demonstrated in the levels of carcass contamination regarding the enumerated microorganisms at any specific phases of the daily slaughter cycle. The lowest contamination, which amounted to 25 jtk/10 cm² in the case of Enterobacteriaceae and 10 jtk/cm² in the case of enterococci, was determined in phase III; the highest - which amounted to 25 jtk/10 cm² and 5.0 × 10¹ jtk/cm², respectively - was determined in phase I. In most of the cases no influence of the day (cycle) in which the examinations were conducted upon the level of contamination of the carcasses with the enumerated microorganisms was confirmed. Only in the fourth (bacteria from the Enterobacteriaceae family) and in the second cycles (Enterococcus bacteria) a significantly lower level of contamination was confirmed in comparison with the first cycle. The logarithmic daily averages of contamination with these microorganisms ranged from 0.2 to 0.96 log jtk/cm² (15 jtk/10 cm² - 9 jtk/cm²) as well as 0.76 - 1.76 log jtk/cm² (6 - 5.8 × 10¹ jtk/cm²), respectively. Likewise in the case of bacteria from the Enterobacteriaceae family the logarithmic daily averages did not exceed boundary values for the number of microorganisms (m) determined for the hygiene standard for the sheep slaughter process in the order of the Commission 2073/2005. In none of the examined samples were Salmonella rods detected. The conducted study demonstrated no influence stemming from the number of slaughtered animals during the course of the slaughter day on the level of bacterial contamination on the surface of carcasses. This results from following the procedures and principles of the HACCP system as well as a properly conducted sanitary-veterinary supervision of the investigated slaughter house.
The aim of the study was to evaluate the bacterial contamination of beef carcass surfaces in relation to the order of slaughtering cattle during a slaughter day. The research was conducted on carcasses from a slaughterhouse approved to produce for the market. The daily average number of cattle slaughtered in the facility was 237. For the purpose of determining the total count of aerobic bacteria, bacteria from the Enterobacteriaceae family and Enterococci, 40 carcasses were examined (8 per each working day), whereas 50 carcasses (10 per each working day) were used to detect the presence of Salmonella rods. The microbiological denotation of particular bacterial groups was conducted according to the relevant Polish Standards. Samples for examination were collected by the destructive technique and swabbing (with sterile swabs) from the rump, neck, plate and chuck of carcasses before chilling, in accordance with the Polish Standards. It was found that the order of slaughtering cattle during a slaughter day had no significant influence on the total bacterial count on the examined carcass surfaces. The total count of aerobic bacteria in beef carcasses ranged from 1.8 × 10² cfu/cm² (2.26 log cfu/cm²), at the first stage of the slaughter process, to 5.9 × 10² cfu/cm² (2.77 log cfu/cm²), at the third stage of the slaughter process. In most cases no relationship was observed between the total bacterial count and the day of the week on which the examination took place. The daily log mean value of contamination fell within the range of 3.5 × 10² (2.55 log cfu/cm²) to 8.3 × 10² cfu/cm² (2.92 log cfu/cm²). Only on the third working day the total aerobic count was found to be significantly lower (8.7 × 10¹ cfu/cm² - 1.94 log cfu/cm²) than on the fourth day. The values determined were lower than the hygienic standards for the slaughter process set in Commission Regulation (EC) No. 2073/2005 by about 1 to 0.4 log (and as much as 1.4 log on the third working day), respectively. The bacteria from the Enterobacteriacae family were found in 23% of samples examined and Enterococci in 45%. Contamination with the above bacteria was insignificant as it did not exceed 8 cfu/cm² for Enterobacteriacae and 3.2 × 10¹ cfu/cm² for Enterococci. None of the samples examined was found to be contaminated with Salmonella rods. Neither the order of slaughtering cattle during a slaughter day nor the day of the week on which the examination took place affected the total bacterial contamination of carcasses. The standard of sanitary supervision in the slaughterhouse was good.
The aim of the study was evaluating the bacterial contamination of surface carcasses in relation to the order of slaughtering on the day of slaughter. The total bacteria count as well as the enterobacteriaceae, enterococci and Salmonella rods were described. The total count of aerobic bacteria in the swine carcasses vacillated from 4.3 × 103 to 8.7 × 103 microorganisms per 1 cm2. Significant differences in the level of contamination of the examined carcasses were not discovered. Enterobacteriaceae were confirmed in 8 samples (27%) and enterococci in 9 (30%). Salmonella rods were not determined in the examined samples. The order of slaughter of the swine did not influence the general level of bacterial contamination of the carcasses. The level of the sanitary inspection at the examined slaughter house was at a good level.
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