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1

Establishment of transgenic marigold using the floral dip method

100%
Cheng X., Huang C., Zhang X., Lyu Y.
Acta Physiologiae Plantarum
|
2019
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tom 41
|
nr 08
The floral dip transformation method avoids tissue culture and regeneration processes of marigold, and the green fluorescent protein (GFP) is widely used in the detection of genetically modified organisms. In our study, the binary vector PCB260 containing the screening gene basta, the reporter gene GFP, and a 4 × 35S enhancer was transferred into marigold (Tagetes erecta) via Agrobacterium tumefaciens EHA105-mediated transformation using the floral dip method. After herbicide-resistance screening and genomic PCR testing, four transgenic lines were obtained in T₀ generation. In the T₁ generation, 15 transgenic plants showed fluorescence and were GFP-positive with phenotypic changes. The segregation ratio of mutant to normal plants was 1:3. Plant height, leaf length and width were significantly greater in the normal plants than in the mutant plants. Mutant plants did not bloom.
2

Variations of cuticular wax in mulberry trees and their effects on gas exchange and post-harvest water loss

88%
Ni Y., Sun Z., Huang X., Huang C., Guo Y.
Acta Physiologiae Plantarum
|
2015
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tom 37
|
nr 06
Though mulberry (Morus alba) tree shows great adaptations to various climate conditions, their leaf water status and photosynthesis are sensitive to climate changes. In the current study, seven widely planted mulberry cultivars in Chongqing, Southwest China, were selected to analyze leaf cuticular wax characteristics, gas exchange index, post-harvest leaf water status and their relationships, aiming to provide new theory in screening high resistant mulberry cultivars. Mulberry trees formed rounded cap-type idioblasts on the adaxial leaf surface. Film-like waxes and granule-type wax crystals covered leaf surfaces, varying in crystal density among cultivars. The stomatal aperture on the abaxial surface of cultivars with high wax amount was smaller than that of cultivars with low wax amount. The amount of total wax was negatively correlated with the net photosynthetic rate (PN), transpiration rate (E) and stomatal conductance (gs) and positively correlated with the moisture retention capacity. It suggested that both cuticular wax and stomatal factor might be involved in regulating water loss in mulberry leaves under field conditions. The variability in moisture retention capacity and cuticular wax characteristics might be important in evaluating and screening mulberry cultivars for increasing silk quality and silkworm productivity.
3

Isolation and characterization of novel human short-chain dehydrogenase-reductase SCDR10B which is highly expressed in the brain and acts as hydroxysteroid dehydrogenase

88%
Huang C., Wan B., Gao B., Hexige S., Yu L.
Acta Biochimica Polonica
|
2009
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tom 56
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nr 2
279-289
Hydroxysteroid dehydrogenase belongs to the subfamily of short-chain dehydrogenases/reductases (SDR), and 11-β-hydroxysteroid dehydrogenase catalyzes the interconversion of inactive glucocorticoids (cortisone in human, dehydrocorticosterone in rodents) and active glucocorticoids (cortisol in human, corticosterone in rodents). We report here the cloning and characterization of a novel human SDR gene SCDR10B which encodes a protein with similarity to 11β-hydroxysteroid dehydrogenase 1. SCDR10B was isolated from a human brain cDNA library, and was mapped to chromosome 19p13.3 by browsing the UCSC genomic database. It contains an ORF with a length of 858 bp, encoding a protein with a transmembrane helix and SDR domain. Its molecular mass and isoelectric point are predicted to be 30.8 kDa and 10.3 kDa, respectively. SCDR10B protein is highly conserved in mammals and fish. Phylogenetic tree analysis indicated that SCDR10B stands for a new subgroup in the 11β-hydroxysteroid dehydrogenase family. Northern blot analysis showed that SCDR10B was highly expressed in brain, and a strong expression signal was detected in hippocampal neurons by immunohistochemical analysis. RT-PCR and immunohistochemical analysis showed that SCDR10B was up-regulated in lung-cancer cell lines and human lung cancer. SCDR10B can catalyze the dehydrogenation of cortisol in the presence of NADP+, and therefore it is a hydroxysteroid dehydrogenase.
4

Lutein content in petals and leaves of marigold and analysis of lutein synthesis gene expression

88%
Cheng X., Zhao X., Huang C., Zhang X., Lyu Y.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 07
Lutein is a bioactive natural carotenoid that is a member of the oxygen-containing carotenoids, and marigolds are the main raw material for the extraction of lutein. The lutein content in the leaves and petals of eight varieties of marigold including two inbred lines and six nuclear male sterile AB lines was measured using high-performance liquid chromatography (HPLC). The results showed that lutein was mainly stored in the petals of marigold, which had a lutein content 20 times higher than that in the leaves. Additionally, the lutein content in male parent 3 was the highest among the eight varieties of marigold in both petals and leaves. Correlation analysis showed that the expression level of the LCYE gene in the petals was positively correlated with the lutein content, with a correlation coefficient of 0.82, and the expression level of the LCYB gene in the leaves was positively correlated with the lutein content, with correlation a coefficient of 0.77. This study laid the foundation for the evaluation of parental resources and quality breeding of pigment marigold.
5

Quantitative evaluation of soil erosion in the upper Minjiang River Basin of China based on integration of geospatial technologies using RUSLE

76%
Ye H., Hua W., Huang C., Huang T., Wang H., Yuan X., Yang H.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Pharmacokinetics of diclofenac sodium injection in swine

76%
Yang H. F., Li Y. J., Li Y. Y., Huang C., Huang L. X., Bu S. J.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 2
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Abundance and community structure of ammonia oxidizing archaea and bacteria in response to peanut growth under controlled condition in Shandong, China

76%
Huang C., Xu N., Fu Z., Sun B., You S., Ma D., Yu J.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
|
nr 6
Based on a three-year field experiment under controlled condition in Ji’nan, China, the effects of peanut growth on the variation in the abundance and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA) before and after peanut growth were investigated through quantitative PCR and cluster analysis of terminal-restriction fragment length polymorphism. Our results show that the community composition of AOA and AOB was greatly affected by the peanut growth leading to the decreased abundance of AOA and increased abundance of AOB. Furthermore, AOA and AOB community structures varied before and after peanut growth. Phylogenetic analysis indicated that all AOA and AOB community sequences were clustered into the uncultured group. Altogether, the results suggested that the abundance of AOA and AOB in soil and their community compositions can be greatly affected by the peanut growth.
8

Repairing compound-contaminated soil using EK-laccase combination repairing technology

64%
Wang Z., Ren D., Dai X., Kang C., Zhang S., Zhang X., Deng Z., Huang C., Guo H.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 3
9

Effect of polyhydroxyl compounds on the thermal stability and structure of laccase

64%
Kang C., Ren D., Zhang S., Zhang X., He X., Deng Z., Huang C., Guo H.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
In this study, glucose, inositol, glycerol, and sorbitol have been chosen as chemical modifiers to enhance laccase stability. The regularity of laccase activity variations was studied at different temperatures. Low concentrations of four polyhydroxyl compounds can improve the thermal stability of laccase at the whole temperature gradient (20~60ºC). The effect of glycerol is best in the all additives, and the relative enzyme activity of laccase increased 1.35 times by glycerol, in the concentration of 0.6 mol/L. The conformation of modified laccase is characterized by UV, fluorescence and circular dichroism. The result shows that the polyhydroxy compounds can change the conformation of laccase by increasing the hydrogen bonds between the laccase molecules and prevent the adverse change due to the increase of temperature in the secondary structure of laccase. Therefore, low concentrations of polyhydroxy compounds could be served as an additive to improve the thermal stability of the laccase.
10

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase-reductase 9

64%
Liu S., Huang C., Li D., Ren W., Zhang H., Qi M., Li X., Yu L.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 1
We report here the cloning and characterization of a novel human short-chain dehydrogenases/ reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.
11

Enrichment process and efficient removal of thallium from steel plant desulfurization wastewater

52%
Liu J., Lin Y., Zhang W., Yin M., Wang J., Li N., Luo X., Wei X., Chen Y., Wu Y., Liu S., Yu X., Wu X., Zhang W., Huang C.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
Thallium (Tl) is a typical trace metal of extreme high toxicity. As a concomitant element, Tl is widely found in various sulfide minerals and K-containing rock minerals. The outburst of Tl pollution in drinking water sources of the northern branch of the Pearl River in China as reported in 2010 has greatly aroused public concerns about Tl pollution in China. Apart from typical sources of Tl pollution such as Pb and Zn smelting and the mining and utilization of Tl-containing pyrite ores, the steel-making industry was discovered a new significant source that contributed to this Tl pollution incidence. Thallium contents in raw materials, fly ash and wastewater collected from a typical steel-making enterprise were determined by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that Tl contents (0.02-1.03 mg/kg) are generally low in the raw materials, while fly ash samples have generally enriched Tl levels (1.31-6.45 mg/kg). Wastewater obtained from the dedusting process of the sintering furnace also exhibited excessive Tl levels (574-2130 μg/L). All these results suggested a possible release and gasification of Tl compounds from the raw materials under high temperatures (>800ºC) during the sintering processes, which were then accumulated in the flue gas and fly ash and washed into the wastewater by wet dedusting. Lime precipitation method is not effective for removing Tl from wastewater, since Tl mostly is present as dissolved Tl⁺ in the water. The study initiated a preliminary design of a fast and effective treatment method for Tl removal from Tl-containing industrial wastewater by using a deep oxidation system.
12

A Survey on How Ocular Surface Demodex Infestation Interactively Associates with Diabetes Mellitus and Dry Eye Disease

52%
Huang C., Chen S., Fu S., Li Y., Li Z., Li S., Liang X., Wang Z., Wang Z., Chen Y., Deng Q., Yi G., Fu M.
Acta Parasitologica
|
2021
|
tom 66
|
nr 3
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