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1

Bialka lubinu indukowane jonami olowiu i innymi czynnikami stresowymi

100%
Przymusinski R.
Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu
|
2003
|
nr 68
90
2

Immunohisto- and cytochemical localization of PR-10 proteins induced by heavy metals in lupine roots

63%
Przymusinski R., Gzyl J.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 05
Most of the data concerning PR-10 proteins accumulation in plants under various environmental stress conditions are derived from biochemical analysis of transcript expression or polypeptide accumulation. In the current study, we examined the localization of PR (L1–L6) proteins, belonging to the PR-10 class family, in the root tips of yellow lupine seedlings using histo- and cytochemical methods. Immunohistochemical localization revealed the presence of PR-proteins in all examined yellow lupine root tip tissue, and lead treatment did not change the distribution of proteins in the roots. However, the metal induced a significant increase in the accumulation of PR-proteins in the meristematic cells and procambium tissue. An immunocytochemical approach with immunogold method demonstrated the cytoplasmic nature of the PR-proteins and their increased accumulation after copper treatment. The results confirmed the affiliation of PR (L1– L6) to PR-10 class family proteins, as well as their intracellular localization.
3

Organospecific responses of lupin seedlings to lead. Localization of hydrogen peroxide and peroxidase activity

51%
Przymusinski R., Rucinska-Sobkowiak R., Ilska B., Gwozdz E. A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 5
In an earlier work using tissue printing method, we found that the PR-10 stress protein was observed in leaf petiole of lupin seedling where lead was not detected (Przymusiński et al. 2001). These results suggested the presence of substance(s) mediating a signal transduction from directly affected cells to distant organs. As the hydrogen peroxide was found to be involved in signal transduction pathway, in the present paper, we analysed the level of H₂O₂ in the organ of lupin seedlings exposed to Pb²⁺ with spectrophotometric method and tissue printing technique. It was unequivocally demonstrated that the level of H₂O₂ and the activity of peroxidase increased in every tested organ of lead-treated lupin seedling. Both the level of H₂O₂ and the activity of POX were correlated with amount of Pb²⁺ ions in the cells (Przymusiński et al. 2001) and decreased in tissues more and more distant from the site of metal application. On the other hand, there was no correlation between the histological localization of H₂O₂ and peroxidase. Our results seem to confirm the hypothesis that H₂O₂ may act as a signalling substance involved in the induction of PR protein synthesis. It was indicated that there is high degree of correlation between the localization of H₂O₂ and the histological localization of PR-10 proteins (Przymusiński et al. 2001) in every tested organ of lupin seedling. The presented hypothesis is also supported by the fact that H₂O₂ and PR-10 proteins are detected in organs and tissues where Pb²⁺ was not found at all.
4

The peroxidase activity in lead-treated lupin roots

51%
Rucinska R., Przymusinski R., Kolodziejska A., Gwozdz E. A.
Biological Bulletin of Poznań. Supplement
|
1995
|
tom 32
5

Stress proteins and antioxidant enzymes in lupin seedling exposed to lead

51%
Przymusinski R., Rusinska R., Gwozdz E. A.
Acta Physiologiae Plantarum
|
1999
|
tom 21
|
nr 3 suppl.
6

Changes in proline level and polypeptide pattern in lupin roots exposed to salt stress

51%
Zimna-Zarnowska A., Swiercz B., Przymusinski R., Gwozdz E.
Biological Bulletin of Poznań. Supplement
|
1995
|
tom 32
7

Organospecific reactions of Yellow lupin seedlings to lead

51%
Gzyl J., Przymusinski R., Wozny A.
Acta Societatis Botanicorum Poloniae
|
1997
|
tom 66
|
nr 1
61-66
8

The effect of kinetinon cytochemical localization of Mg ++ dependent ATP-ase in isolated lupine cotyledons

51%
Przymusinski R., Wozny A., Mlodzianowski F.
Acta Societatis Botanicorum Poloniae
|
1981
|
tom 50
|
nr 4
9
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Gamma-tubulin distribution in root cells of soybean (Glycine max L.) seedlings under cadmium stress

51%
Gzyl J., Przymusinski R., Gwozdz E. A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
10

Changes of H2O2 level in lupine seedlings exposed to lead

51%
Przymusinski R., Bartczak J., Gwozdz E. A.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 3 suppl.
11

Lupin root responses to heavy metals

51%
Przymusinski R., Rucinska R., Gwozdz E. A.
Biological Bulletin of Poznań. Supplement
|
1996
|
tom 33
12

Plant cell responses to heavy metals: molecular and physiological aspects

51%
Gwozdz E. A., Przymusinski R., Rucinska R., Deckert J.
Biological Bulletin of Poznań. Supplement
|
1997
|
tom 34
13

Changes of H2O2 level in organs of lupine seedlings exposed to lead

51%
Przymusinski R., Bartczak J., Gwozdz E. A.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
14

Plant cell responses to heavy metals: molecular and physiological aspects

51%
Gwozdz E. A., Przymusinski R., Rucinska R., Deckert J.
Acta Physiologiae Plantarum
|
1997
|
tom 19
|
nr 4
The effect of lead, cadmium and cooper on protein pattern, free radicals and antioxidant enzymes in root of Lupinus luteus L. were investigated. Heavy metals inhibited growth of lupin roots, which was accompanied by increased synthesis and accumulation of a 16 kDa polypeptide (Przymusiński et al. 1991 Biochem. Physiol. Pflanzen., 187:51–57). This component has been earlier identified as immunologically related to Cu,Zn-superoxide dismutase (Przymusiński et al. 1995 Env.Exp.Bot., 35:485–495). However, more detailed study revealed that this stress-stimulated protein is composed of four to six polypeptides of different electrophoretic mobility. The most abundant polypeptides of the 16kDa region were found to be closely homologous to pathogen related proteins. The number and intensity of these polypeptides was highly variable in roots of individual seedlings, which suggests that they might represent separate allelic forms. Electron paramagnetic spectra revealed that at low lead concentrations the amplitude of the first derivative was similar to the control and distinctly increased at higher metal concentrations. On the other hand, at the lower lead concentrations the activity of antioxidant enzymes increased, whereas at higher metal doses the enzyme activities did not raise further (SOD) or even dropped (CAT, APOX). This implies that the responses of antioxidant system to lead is dose-dependent stimulated by low metal concentrations, whereas at the higher metal level the free radical emission is beyond the quenching capacity of antioxidant enzymes, which in turn might contribute to the reduced root growth. The effect of various heavy metals: Pb²⁺, Cd²⁺ and Cu²⁺ on phytochelatins and antioxidant enzymes depends on the kind of metal ion. Pb²⁺ and Cd²⁺ stimulated the PCs formation whereas Cu²⁺ was not effective. On the other hand, in root exposed to Cu the activity of catalase (CAT) was the highest as was the production of H₂O₂. The strong oxidative effect of Cu²⁺ ions which were not complexed by PCs suggests that these peptides might by involved in the cellular defense system by binding excessive heavy metal ions. On the basis of our results it can be concluded that in lupin roots exposed to heavy metals there is a complex defense system against metal phytotoxicity, which comprises of specific proteins, antioxidant enzymes and phytochelatins.
15

Organospecific responses of lupin seedlings to lead. I. Localization of lead ions and stress proteins

51%
Przymusinski R., Banaszak A., Gwozdz E. A.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 1
16

Plant cells responses to heavy metals

45%
Gwozdz E. A., Rucinska R., Przymusinski R., Sobkowiak R., Deckert J.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
17

The effects of lead on the organization of cytoskeleton in lupin root cells

45%
Gzyl J., Kwasnik E., Samardakiewicz S., Przymusinski R., Gwozdz E. A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
18

Physiological and molecular aspects of plant cell responses to heavy metals

33%
Gwozdz E. A., Deckert J., Przymusinski R., Rucinska-Sobkowiak R., Pawlak S., Arasimowicz-Jelonek M., Kopyra M., Gzyl J., Sobkowiak R.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1 Suppl.
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