Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 12

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Attempts of using stallions naturally infected with Equine arteritis virus in reproduction The objective of the studies was to check both in vitro and vivo the use in reproduction of convalescent stallions of semen free from the Equine arteritis virus (EAV). The examinations were done on 92 stallions from the stud К in which sera of 83.2% of animals were free from antibodies to EAV. Three stallions used for mating in 1993 were seropositive. Attempts to isolate the EAV from semen were performed on RK-13 and Vero cell cultures. From the semen of one stallion a virus identified as AEV by the direct immunofluorescence test was isolated. As for the two remaining convalescent stallions with negative results of the laboratory test for EAV, semen was examined for the EAV transfer on four fully susceptible mares.
On the surface of the skin of a dog aged 9 months some noduli of different size were found. They were painless, of compact consistency, movable in relation to the base and immobile in regards to the skin. Haematological examinations revealed an increased amount of lymphocytes up to 44 per cent, a decreased number of neutrocytes (up to 35 per cent) and the appearance of mononuclear and blastic cells, as well as myelocytes. Histopathological examinations showed the infiltration of dermis and subdermis layers with cells of the lymphoblast type. It was found that the changes were caused by lymphosarcoma which is relatively rarely met in dogs.
In the study flow cytometry was attempted to identify EAV infection in stallions’ semen. Material for the study consisted of 8 semen samples taken from EAV-seropositive stallions. The samples were put on RK-13 and Vero cells. After a 24-hour incubation period the cells were treated with a specific conjugate and then analyzed in FACS aparatus. In the case of two samples positive fluorescence was observed. The above positive result was confirmed by virus isolation in both cell cultures used.
The aim of the study was to determine the frequency of bacterial and mycologic flora in dogs with otitis externa and its sensitivity to selected drugs. Swabs were taken trom 92 dogs with signs of otitis externa. The following strains were isolated from 87 dogs: S. intermedius (51), E. colt (13), Streptococcus spp. (9), P. vulgaris (8), S. aureus (1), S. simulans (1), P. mirabilis (1), K. pneumoniae (1), and P. pachydermatis (36). Gram-positive bacteria were sensitive to gentamicin (91.9%), amikacin (90.3%) and ce- fradine (72.6%), while an increased resistance was found to hnkomycin, penicillin, doxycycline and ampicillin. Gram-negative bacteria proved to be sensitive to amikacin (87 1%) metylmicin (83.8%), gentamicin (67.7%), and partly to doxycycline, cefradine, chloramphenicol and amoxicilline. The isolated strains of P. pachydermatis were most sensitive to ketoconazole (100%) and mycoconazole (88.9%). They were relatively resistant to pimaricine, nystatin and amphotericin B.
The study was carried out on an Anglo-Arab horse stud "O" at which 186 horses were kept including 67 brood-mares and 8 stallions. During tne 1991 breeding season the semen from the four stallions was used for insemination. At the begining of April 1991, insemination with semen from the stallion ARC, who was brought to the stud "O" a month earlier, was carried out. About seven days later, fever (39.5 - 40.5°C) and anorexia appeared in some of the inseminated mares. In most of the feverish mares, edema of eyelids and reddening of conjunctivas were also found. Two mares aborted after 3-4 months of gestation. Gynecological examination with an ultrasonograph was performed three times: before, at the begining and after the enzootic of Equine Viral Arteritis and showed permanent increase of embrionie death and resorption up to 31.8%. From tissue samples of two aborted fetuses and ARC's semen Equine Arteritis Virus was isolated. In serological examination for the presence of neutralizing antibodies against EAV conducted in 1989 on a group of 71 horses from the stud "O", 9.8% positive reactions were found. Subsequent serological examination performed on a group of 186 horses in 1991 after introduction of the stallion ARC to the stud revealed that 67.7% of animals were positive for EAV antibodies.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.