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Due to certain properties of ultra violet rays they are primarily used for sterilizing a surface or the air. UV rays are used, among others, to disinfect isolation wards for sterile work, laminar flow cabinets, bacteriological and virusological laboratories, operating rooms, rooms in animal houses, as well as the surfaces of tables, walls or floors. The evaluation of bacterial pollution as well as the effectiveness of methods for sterilizing the air remain relevant problems in many fields, e.g. in industry, animal production, investigative laboratories or health clinics. The aim of the study was to evaluate the degree that the level of bacterial air pollution was reduced in a cabinet for microbiological investigations, sterilized by a UV lamp with a 30 watt florescent lamp. Sampl'air Lite (AES Laboratoire Chemunex) apparatus was used to gather air from the atmosphere. Samples were sampled after 15, 30, 45, 60, 75, 90, 120, 150 and 180 minutes of sterilizing the air in the cabinet with 6 repetitions for each period of irradiation. The initial and final level of the bacterial air pollution in the cabinet for microbiological investigations in relation to the period of the action of the UV rays as well as the level of bacterial reduction were evaluated. The minimal period of the action of the UV rays that ascertained a statistically significant and simultaneously the largest reduction of the bacterial air pollution was 180 minutes. With regards to all the remaining periods of irradiation no essential differences in the levels of bacterial reduction were noted, despite considerable (1.7-4.0 times) reductions of bacteria. An essential correlation was indicated between initial air pollution in the cabinets and 15, 45, and 60 minute periods of the action of UV rays. The correlation coefficients were 0.95 (p ≤ 0.01), 0.82 (p ≤ 0.05) and 0.96 (p ≤ 0.01), respectively. In the remaining cycles of the experiment the correlations were not statistically significant, and the correlation coefficients include in the interval of 0.45-0.60. There is a need, also noted by other authors, of unifying acceptable levels of microbiological air pollution for the purposes of laboratory investigations and production processes.
The objective of the presented study was to determine microbial contamination of calf muscle tissues in relation to slaughtering process of calves during a slaughter day. In order to determine the total aerobic bacteria count, and the presence of bacteria from the Enterobacteriaceae family and enterococci, 32 carcasses were examined (eight each slaughter day), while 40 carcasses (10 each slaughter day) were examined for the detection of Salmonella organisms. Microbiological evaluation of each bacterial group was performed according to the Polish Norms. In most cases, no significant differences were reported between the total aerobic counts on calf carcass surfaces as determined at each slaughter cycle. The daily log mean values were lower by 1 up to 1.5 log, respectively , than the maximal bacteria count (M). Bacteria from the Enterobacteriaceae family were isolated from 59.4% of the evaluated samples. However, contamination with these bacteria was insignificant - 1.7 x 10 cfu/cm². Enterococci were isolated from all investigated samples and contamination ranged from 1.1 x 10 cfu/cm² up to 4.1 x 10² cfu/cm². Salmonella strains were not found in any of the evaluated samples. The order of the slaughtering calves during a slaughter day and the day of the week when the examination was performed did not have any influence on total microbial contamination of carcasses. Thus, sanitary conditions in the examined abattoir were satisfactory and slaughter process was conducted at a high quality level.
The aim of the research was to determine chosen sensory features of rabbit muscle tissue and their variability in relation to breed and the part of carcass. The research material comprised the carcasses of approximately 20-week-old rabbits. The evaluation concerned the carcasses of crossbreeds and a meat breed (French Lops). The entire carcass was evaluated in terms of its appearance and aroma. The evaluation was performed directly after slaughter and after a 24-hour cold storage. The juiciness, tenderness and general assessment of muscle tissue from the leg and saddle were established after 24 hours. The sensory analysis was performed by a 6-member commission with the use of an extended 5-point scale. An additional evaluation of tenderness was carried out with the texture analyser TA.XTplus. The muscle tissue of both the crossbreeds and French Lops was light pink, glossy and uniform on its entire surface. The aroma was imperceptible or faint, characteristic of the breed. No significant differences in the appearance and aroma of muscle tissue were observed after a 24-hour cold storage. Juiciness and tenderness depended on the kind of muscles under examination; leg muscles received higher marks. In the general evaluation leg meat was also appraised more highly than that of the saddle. The juiciness, tenderness and general evaluation of French Lop meat were definitely lower as compared to crossbreeds; the differences were demonstrated by both the sensory evaluation and apparatus measurements.
The aim of the study was to determine the bacterial contamination of lamb carcasses in relation to the number of slaughtered animals during a day of slaughtering. The investigations were conducted on carcasses derived from a slaughterhouse that had accreditation for market production. In the investigated slaughterhouse the slaughter of the animals is carried out in one cycle, during one day of the week (most frequently on Mondays). The average number of animals slaughtered at the facility comes to 164 lambs. In order to determine the general number of aerobic bacteria, bacteria from the Enterobacteriaceae family and fecal streptococcus, 32 carcasses were examined (8 from each slaughter cycle); in order to determine the presence of Salmonella rods, 50 carcasses were examined (12 in 3 slaughter cycles and 14 in the fourth). Determining specific groups of microorganisms was conducted in accordance with Polish Norms (PN). Samples for examination were collected by the destructive and non-destructive methods (swabbing technique with sterile cotton swab) from the chuck steaks, saddle, brisket and thigh in accordance with PN. Each daily slaughter cycle was divided into 4 phases. In phase I the number of slaughtered animals did not surpass 25% of the average number of animals scheduled for slaughter in a given day, in phase II - the number was within the 51-60% division, in phase II - the number was within 61-74%, and in phase IV it was always at a level above 75%. In every phase samples were gathered from 2 carcasses (n = 8). In order to calculate the logarithmic daily average for the examined slaughter cycles (days) the results gained for the samples gathered in the II, III and IV phases of slaughter (n = 6) were taken into account. The obtained results were statistically analyzed with the average value and standard deviation calculated. The variability factor was determined on the basis of an analysis of the variance through the application of T-Tukey’s multiple confidence intervals for p ≤ 0.05. The investigation confirmed that the order of the slaughter of lambs in the course of the slaughter day does not significantly influence the level of the general number of bacteria on the surface of the examined carcasses. The results demonstrated that the general contamination of the lamb carcasses with aerobic bacteria vacillated from 8.0 × 10² jtk/cm² (phase III) to 2.9 × 10³ jtk/cm² (phase I). Nor were essential differences between specific slaughter phases confirmed within the range of the general number of microorganisms. The contamination was within the division of 7.5 × 10² (2.87 log) to 2.0 × 10³ (3.34 log) jtk/cm². The obtained logarithmic daily averages of contamination did not exceed boundary values for the number of microorganisms (m) determined for the hygiene standard for the sheep slaughter process in the order of the Commission 2073/2005. Bacteria from the Enterobacteriaceae family were confirmed in 18 samples (56.25%), and fecal streptococcus in all the examined samples. No significant differences were demonstrated in the levels of carcass contamination regarding the enumerated microorganisms at any specific phases of the daily slaughter cycle. The lowest contamination, which amounted to 25 jtk/10 cm² in the case of Enterobacteriaceae and 10 jtk/cm² in the case of enterococci, was determined in phase III; the highest - which amounted to 25 jtk/10 cm² and 5.0 × 10¹ jtk/cm², respectively - was determined in phase I. In most of the cases no influence of the day (cycle) in which the examinations were conducted upon the level of contamination of the carcasses with the enumerated microorganisms was confirmed. Only in the fourth (bacteria from the Enterobacteriaceae family) and in the second cycles (Enterococcus bacteria) a significantly lower level of contamination was confirmed in comparison with the first cycle. The logarithmic daily averages of contamination with these microorganisms ranged from 0.2 to 0.96 log jtk/cm² (15 jtk/10 cm² - 9 jtk/cm²) as well as 0.76 - 1.76 log jtk/cm² (6 - 5.8 × 10¹ jtk/cm²), respectively. Likewise in the case of bacteria from the Enterobacteriaceae family the logarithmic daily averages did not exceed boundary values for the number of microorganisms (m) determined for the hygiene standard for the sheep slaughter process in the order of the Commission 2073/2005. In none of the examined samples were Salmonella rods detected. The conducted study demonstrated no influence stemming from the number of slaughtered animals during the course of the slaughter day on the level of bacterial contamination on the surface of carcasses. This results from following the procedures and principles of the HACCP system as well as a properly conducted sanitary-veterinary supervision of the investigated slaughter house.
The investigated material comprised rabbit carcasses obtained from a domestics slaughter (H) and two commercial slaughterhouses (L and M). The spoilage process and its dynamics were determined on the basis of bacteriological studies (total aerobic bacteria count, psychrophilic bacteria count, and presence of proteolitic bacteria), pH measurements, ammonia level as well as basic organoleptic characteristics (appearance and odour). All the determinations were performed after 24 h, 72 h, and 144 h of the storage at 2°C ±1°C and relative humidity ca 80%. The studies indicated the effect of hygienic status of a slaughter place on the bacterial contamination level and muscle tissue durability. Higher initial bacterial contamination of the muscle tissue from the slaughterhouse L contributed to the earlier occurrence of the undesirable organoleptic deviations and higher pH and NH₃ values, that evidenced a more rapid progressive spoilage process. After the 144 h storage, the unfavourable sensory properties disqualified comestible usefulness of the carcasses and markedly limited the further cold storage life of the carcasses from the on domestics slaughter and the slaughterhouse M.
The aim of the study was to describe the influence of the age of geese and type of fat on its chemical composition and selected sensory features. The research was conducted on depot and subcutaneous goose fat derived from 20 Biała Kołudzka breed goose carcasses, which were crossbred from two stocks: W11 from the maternal side and W33 from the paternal side (the so called ‘gęś owsiana’). The investigated fat was derived from geese of two age groups. The first group were young fowl, slaughtered at the age of 16-18 weeks, the second group of 3-4-year-old fowl, slaughtered after their reproductive period. For the examined raw fat the basic composition was described (protein content, fat, water, ash and fatty acids), features that are useful for storage (percentage of free fatty acids, acid and peroxide count) as well as the sensory quality of the fat on the basis of the intensity and desirability of the smell. It has been affirmed that the age and type of fat are essential factors leading to varied chemical compositions, fatty acid profiles and smell of goose fat. In the case of chemical composition the influence of age is most pronounced on subcutaneous fat, which in young fowl contains more protein, water and ash and less fat. The fatty acid profile is also significantly varied. Fat from older geese scores higher regarding nutritional value for both types of fat. This fat contains less saturated fats and more single and multi-unsaturated fats in comparison with fat from younger geese. At the same time, both subcutaneous and depot fat of older geese is less favorably valued by consumers with regards to sensory features.
The aim of the study was to evaluate the bacterial contamination of beef carcass surfaces in relation to the order of slaughtering cattle during a slaughter day. The research was conducted on carcasses from a slaughterhouse approved to produce for the market. The daily average number of cattle slaughtered in the facility was 237. For the purpose of determining the total count of aerobic bacteria, bacteria from the Enterobacteriaceae family and Enterococci, 40 carcasses were examined (8 per each working day), whereas 50 carcasses (10 per each working day) were used to detect the presence of Salmonella rods. The microbiological denotation of particular bacterial groups was conducted according to the relevant Polish Standards. Samples for examination were collected by the destructive technique and swabbing (with sterile swabs) from the rump, neck, plate and chuck of carcasses before chilling, in accordance with the Polish Standards. It was found that the order of slaughtering cattle during a slaughter day had no significant influence on the total bacterial count on the examined carcass surfaces. The total count of aerobic bacteria in beef carcasses ranged from 1.8 × 10² cfu/cm² (2.26 log cfu/cm²), at the first stage of the slaughter process, to 5.9 × 10² cfu/cm² (2.77 log cfu/cm²), at the third stage of the slaughter process. In most cases no relationship was observed between the total bacterial count and the day of the week on which the examination took place. The daily log mean value of contamination fell within the range of 3.5 × 10² (2.55 log cfu/cm²) to 8.3 × 10² cfu/cm² (2.92 log cfu/cm²). Only on the third working day the total aerobic count was found to be significantly lower (8.7 × 10¹ cfu/cm² - 1.94 log cfu/cm²) than on the fourth day. The values determined were lower than the hygienic standards for the slaughter process set in Commission Regulation (EC) No. 2073/2005 by about 1 to 0.4 log (and as much as 1.4 log on the third working day), respectively. The bacteria from the Enterobacteriacae family were found in 23% of samples examined and Enterococci in 45%. Contamination with the above bacteria was insignificant as it did not exceed 8 cfu/cm² for Enterobacteriacae and 3.2 × 10¹ cfu/cm² for Enterococci. None of the samples examined was found to be contaminated with Salmonella rods. Neither the order of slaughtering cattle during a slaughter day nor the day of the week on which the examination took place affected the total bacterial contamination of carcasses. The standard of sanitary supervision in the slaughterhouse was good.
The aim of the study was evaluating the bacterial contamination of surface carcasses in relation to the order of slaughtering on the day of slaughter. The total bacteria count as well as the enterobacteriaceae, enterococci and Salmonella rods were described. The total count of aerobic bacteria in the swine carcasses vacillated from 4.3 × 103 to 8.7 × 103 microorganisms per 1 cm2. Significant differences in the level of contamination of the examined carcasses were not discovered. Enterobacteriaceae were confirmed in 8 samples (27%) and enterococci in 9 (30%). Salmonella rods were not determined in the examined samples. The order of slaughter of the swine did not influence the general level of bacterial contamination of the carcasses. The level of the sanitary inspection at the examined slaughter house was at a good level.
The purpose of the study was to determine microbial contamination of mutton carcass surface with regard to the number of the slaughtered animals. The total bacterial load and Enlerobacteriaceae and enterococci counts were determined. Sampling for microbiological analysis as well as detection and enumeration of each microorganism group were performed according to the Polish Standards. No significant effect of the order of the slaughtering animals during the slaughter day on total bacterial count on mutton carcass surfaces was found. The overall bacterial contamination of mutton carcasses were between 1.0 × 10³ cfu/cm² (3.0 log - stage I) and 2.5 × 10³ cfu/cm² (3.4 log - stage III). No significant difference among the slaughter cycles, as indicated by total microbial numbers was observed. The obtained daily mean log values ranged from 4.7 × 10² (2.67 log) and 7.6 × 10³ (3.88 log) cfu/cm². The daily log mean values were lower than the maximal bacteria count (M) set out for hygiene standard of sheep slaughter process by the Commission Regulation 2073/2005. Bacteria belonging to the Enterobacteriaceae family were recovered from 21 (65.6%) samples while enterococci were identified in 28 (87.5%) samples. In most cases, significant differences in the level of contamination with bacteria isolated from the carcasses at each stage of a daily slaughter cycle, were not observed. At stage III, significantly higher levels of bacterial contamination (0.86 and 1.31 log cfu/cm² respectively) were established as compared to stage I (0.37 and 0.58 log cfu/cm² respectively). There were no Salmonella-positive samples determined. Importantly, the number of slaughtered animals during a slaughter day did not influence bacterial contamination on carcass surface if the successful application of HACCP control system was combined with the implementation of optimal sanitary supervision.
Rabbit meat is currently quite popular world-wide, which is influenced by its taste and nutritional values. The meat has a high content of protein, vitamin B, mineral and trace element components, and a low fat and cholesterol content. The evaluation of consumers is positive toward its basic sensory characteristics, such as color, odor, flavor and texture. Rabbit meat is described as delicate, not too stringy, very tender and juicy. The hygienic quality of the slaughtered rabbit carcass depends on the sanitary condition of the slaughterhouse. Pathological bacteria have been sporadically isolated, among them coagulase-positive Staphylococcus, Salmonella and E. coli. The bacterial contamination of the surface of the carcasses obtained through maintaining sanitary standards is 10³-10⁴ cfu in 1 g. A somewhat higher contamination of 10⁵-10⁶ cfu in 1 g occurs in slaughterhouses that do not meet sanitary standards.
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