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Forests are one the main natural factors that regulate and determine climate, weather patterns and amount of CO2 of an area. With rapid industrialization and rapid urbanization there is a significant increase in deforestation and as a consequence rise in global mean surface temperatures. Rapid and unchecked cut down of forest cover has resulted in some of the worst disasters during the last decades. This paper focuses on studying the role of deforestation, its influence on climate change phenomena and its consequences in Pakistan.
The bio-efficacy of four plant leaf extracts viz., apple of sodom, Calotropis procera Aiton; Mexican poppy, Argemone mexicana Linnaeus; Mexican marigold, Tagetes minuta Linnaeus and Indian neem, Azadirachta indica were tested against mustard aphid, Lipaphis erysimi on Indian mustard, Brassica juncea. The highest per cent aphid reduction during first, second and third spray were 28.79, 40.52 and 59.32 at 1 : 10 g/ml; 34.70, 44.49 and 66.14 at 1 : 5 g/ml and 53.88, 64.84 and 100.00 at 1 : 2.5 g/ml with Indian neem. However, Mexican marigold was also effective at highest concentration (1 : 2.5 g/ml) and reduced 96.38 per cent L. erysimi. All the treatments of plant leaf extracts showed insecticidal activity, but Indian neem followed by Mexican marigold reduced the aphid population to a great extent.
To study the response of cabbage butterfly, Pieris brassicae on cabbage, cauliflower, yellow sarson, gobhi sarson and Indian mustard, various life tables i.e., age specific and stage specific life tables have been constructed in controlled conditions (25±1°C, 65±5% R.H. and 12 h L:12 h D). The age specific life-table revealed that P. brassicae required maximum period of 42 days on Indian mustard and minimum 36 days on cabbage to complete the generation. The survivorship decreased gradually from an initial stage of development till culmination of the generation on each host plant. However, mortality showed a significant variation at different development stages of P. brassicae on each host plant. The apparent mortality and mortality survival ratio was found highest at pupal stage (15.91 and 0.19%) on Indian mustard and lowest at pre-pupal stage (3.39 and 0.04%) on cabbage. Similarly, maximum k-value (0.0753) was recorded at pupal stage on Indian mustard and minimum (0.0150) at pre-pupal stage on cabbage. On the other hand, survival fraction was recorded highest (0.97) at pre-pupal stage on cabbage and lowest (0.84) at pupal stage on Indian mustard. The life-table parameters revealed that cabbage is the most preferred food of P. brassicae than other cole crops.
Storage of experience, including learning and memory, is thought to involve plasticity within pre-existing brain circuits. One model for looking at experience-dependent changes is environmental enrichment (EE), which involves exposing animals to a complex novel environment. Animals exposed to EE have previously been shown to exhibit a variety of behavioural and structural alterations in the brain, including decreased stress, improved learning and memory, altered levels of immediate early genes and synaptic change in the visual cortex. We were interested in understanding what regions of the brain are activated during the initial stages of EE. We used the fos-tau-lacZ (FTL) transgenic mouse to examine changes in functional activation throughout the brain after a single exposure to EE. Female C57BL6 FTL mice (n=19) were divided into three groups; enriched, home cage and handled control. We found that early in the process of EE, there was a high level of FTL expression in a series of brain regions in the enriched group compared to the home cage group and the handled control group, indicating that multiple circuits were activated. These regions include the claustrum, infralimbic cortex, hippocampus, amygdala and the hypothalamus. We believe that EE stimulates an initial strong increase in activation of multiple functional circuits. These circuits are presumably involved in the initial response of the animal to the enriched environment.
Detailed knowledge about site-specific aquifer characteristics, subsurface lithology, and groundwater potential can help to determine the depth and location of fresh groundwater quality. The present research study was carried out by conducting 80 vertical electrical sounding surveys (VESs) in Rahim Yar Khan District (RYK), Punjab, Pakistan to distinguish the fresh groundwater aquifer from saline groundwater and to evaluate the aquifer protective capacity (APC) of overburden. 1XID software (Interpex, USA) was used to accomplish the interpretation of VES data. The VES interpreted data was used to prepare spatial distribution maps of aquifer apparent resistivity (AR), layer thickness, longitudinal conductance (LC), and transverse resistance (TR) for the second, third, and fourth subsurface layers using ArcGIS 10.1. The results showed that the greater part of the study area (65%) had four subsurface geo-electric layers. The spatial distribution maps for AR showed that the fresh groundwater quality was present on the northwestern and northeastern sides of the study area for all the layers. The results also indicated that the APC of overburden increased with the increase of depth from the ground surface. Layer 4 with thickness of 57.09 m showed good APC in the northern and central parts with LC values of >0.7 mhos. Similarly, the higher values of TR showed higher yield potential in the north-eastern part as compared to the southern part. Overall analysis indicated that the spatial distribution maps of AR, layer thickness, LC, and TR should be helpful for future groundwater development in terms of quality and quantity.
During our current study we evaluated the effect of ethylenediaminetetracetic acid (EDTA) and Enterobacter sp. CS2 on nickel stress alleviation and phytoextraction by Impatiens balsamina L in spiked soil. Nickel resistant Enterobacter sp. CS2 was isolated from soil polluted by industrial effluents. The I. balsamina seeds primed with Enterobacter sp. CS2 were raised in EDTA-supplemented soil (10 mM) contaminated with 0, 100, 200, and 300 mg kg⁻¹ Ni for 50 days. The effect of different treatments on plant growth attributes, nickel tolerance index, bioconcentration factor, and translocation factor were evaluated. The Ni stress reduced plant growth, carotenoids, and chlorophyll (chl) content. However, higher Ni uptake and proline contents were observed in plants growing in Ni-contaminated soils. The Enterobacter sp. CS2 inoculation further enhanced Ni uptake and proline contents in I. balsamina plants growing under Ni stress. The inoculated plants showed improved shoot length, root length, carotenoid content, chl ‘a’ and ‘b’ contents, root and shoot dry weight. The Ni tolerance index in Enterobacter sp. CS2-assisted plants was much higher compared to un-inoculated ones. The inoculated plants supplemented with EDTA enhanced 39%, 34%, and 30% Ni uptake in roots respectively under 100, 200, and 300 mg kg⁻¹ of Ni treatment as compared with un-inoculated plants. The data regarding bioconcentration factor and translocation factor showed that Ni phytoextraction capability of I. balsamina plants was significantly enhanced with the supplementation of Enterobacter sp. CS2 and EDTA.
This paper evaluates the environmental impacts of various municipal solid waste (MSW) treatment options produced on-site simultaneously with energy and material recovery. The results present a comparison of life cycle assessment (LCA) for municipal solid waste management (MSWM) in five different waste scenarios along with baseline scenario of the Defence Housing Authority (DHA) in Lahore. All scenarios were modeled using EaseTech software. Nine impact categories were assessed and results were presented based on the ASTM D5231-92(2003) characterization method. Results revealed that a material recovery facility (MRF) had low global warming potential (GWP), but lower avoided burdens in other impact categories. The incineration process indicated fewer burdens on the environment such as GWP (-2.086×10⁷ kg CO₂ eq) as compared to landfills (2.461×10⁷ kg CO₂ eq). This was due to lower avoided emissions in the landfill process compared to incineration. The negative values in results represented higher avoided emissions in treatment processes. Bio-gasification avoided CO₂ emissions (-8.053×10⁵ kg CO₂ eq), but showed negative impacts in other categories. Almost all impact categories were high in composting except for freshwater eutrophication. LCA results provided good knowledge for decision makers as a tool to decide what alternative is a better change for sustainable waste management.
Boron toxicity is one of the most important environmental stresses prevailing globally, which limits grain production. In wheat, tolerance to boron toxicity has been manipulated around the Bo1 locus on 7BL chromosome. However, there is a need to identify new diversity in other Triticeae to broaden the germplasm base for genetic improvement and greater tolerance durability. Among these resources, Aegilops tauschii (2n = 2x = 14; DD) accessions have demonstrated significant outputs with their contribution of novel alleles for various biotic and abiotic stresses influencing traits that add valueto wheat improvement. This study demonstrates the performance of 45 D-genome synthetic hexaploids wheats (SHWs) derived from a durum cultivar ‘Decoy’ for their tolerance to boron toxicity at seedling plus adult stages and validates their diversity at the molecular level. The root and shoot growth suppression as percentage of control was taken as the selection criteria for boron toxicity tolerance. Analysis of variance (ANOVA) exhibited ample variability (at P = 0.0001) among genotypes, treatments, and their interaction. Among 45 SHWs analyzed, 11 were found tolerant at the seedling stage, while 12 were found tolerant during adult plant screening. The different tolerant accessions at seedling and adult plant revealed that the tolerant mechanism was different at both stages. Genetic diversity was investigated using SSR marker specific to chromosome 7D. A total of 38 alleles were amplified with a mean value of 3.8 alleles per marker. Analysis of molecular variance (AMOVA) further established significant genetic diversity among different clusters. The potential genomic regions underlying tolerance to boron toxicity were also highlighted by searching sequences of cereal boron tolerance genes in wheat-specific databases. The wheat genome survey sequence and the draft genome sequence of Ae. tauschii proved to be useful genomic resources that identified five different chromosomal regions highly similar to the available boron tolerant genes in cereals. Conclusively, tolerance to boron toxicity in SHWs, genetic variability among the tolerant accessions based on SSRs, and availability of orthologous copies of boron tolerance genes make SHWs as potent genetic resources for wheat improvement against boron toxicity.
 High serum homocysteine (Hcy) level is regarded as an indicator for impairment of folate-dependent methionine cycle and is associated with oxidative stress. In a case control study, we evaluated eighty 3-5 years old Omani children (40 diagnosed with Autism Spectrum Disorder and 40 their age and gender matched controls) for their fasting serum homocysteine levels as a biomarker of Autism Spectrum Disorder (ASD). Serum folate and vitamin B12 status were also evaluated. The serum homocysteine was measured using an enzyme immunoassay (EIA) technique whereas folate and vitamin B12 were measured using an automated random access immune-assay system. The results indicated that mean serum Hcy levels were significantly (P < 0.05) higher in autistic children (20.1 ± 3.3 µmol/L) as compared to controls (9.64 ± 2.1 µmol/L). Significantly (P < 0.05) lower serum folate (1.8 ± 0.4 µg/L) and vitamin B12 (191.1 ± 0.9 pg/mL) levels were observed in autistic children as compared to controls (6.1 ± 0.6 µg/L and 288.9 ± 1.3 pg/mL, respectively). The levels of homocysteine in autistic children were also much higher as compared to normal reference values (5-15 µmol/L). The results suggest that high fasting serum homocysteine and low folate and vitamin B12 levels could be used as clinical biomarkers for an early diagnosis and management of ASD.
Epidemic outbreaks of avian influenza (AI) virus H5N1 have been frequently reported in Egypt during the last nine years. Here we investigate the involvement of AI H5N1 in outbreaks of acute respiratory disease that occurred in several commercial chicken farms in Egypt in 2011, and we describe to the pathology caused by the virus in the course of the outbreak. Twenty-one chicken farms with history of acute respiratory symptoms and high mortalities were screened for AI H5N1. Virus identification was based on hemagglutination inhibition test, and PCR detection and sequencing of the hemagglutinin and neuraminidase genes. Virus distribution was determined by immunohistochemical staining of AI antigens in organs of infected birds. Standard H&E staining was performed for histological examination of affected organs. Eighty-one % of the examined birds, representing 100% of the screened farms, were positive for AI H5N1 virus. Phylogenetic analysis of the hemagglutinin and neuraminidase genes of the isolated virus reveals its affiliation to clade 2.2.1. Viral antigens were localized in the endothelial cells of the heart, liver, lungs and skin, where pathological lesions including congestion, hemorrhages, multifocal inflammation and necrosis were concurrently observed. According to the pattern of the viral antigen and lesion distribution in the visceral organs, we suggest cardiovascular and circulatory failures as the probable cause of death during these outbreaks. In conclusion, the present study further confirms the epidemic status of AI H5N1 virus in Egypt and reveals the highly pathogenic nature of the local isolates.
The present research deals with the synthesis and viability of Sawdust biochar/Fe3O4 nanocomposite toward textile waste water treatment. The structure, functionality, and morphology of the nanocomposite were determined by FT-IR, powder XRD, and SEM. Removal of the textile dye Reactive Blue 21 (RB21) was almost 75% under optimum conditions. The value of Langmuir dimensionless separation factor RL indicated a favorable adsorption (01), hence denoting favorable sorption. The negative values for free energy indicated a spontaneous reaction. Positive value for enthalpy suggested the endothermic nature of adsorption. The kinetic data showed a pseudo first-order kinetic model. The calculated value of qt agreed with the experimental value of qt at equilibrium. The results depicted that sawdust biochar/Fe3O4 nanocomposites are efficient adsorbents for removing RB21 and has potential application in treatment of textile wastewater.
The basic aim of our research was to reduce water consumption in the dying process in the textile industry. This study evaluated the treatment efficiency of combined process of chemical coagulation and electrocoagulation on the synthetically produced dyes C.I. Reactive Yellow 145 and C.I Reactive Red 194. The wash-off liquor generated during the dyeing process was decolorized by adding a mixture of alum and chitosan, followed by electrocoagulation and reused for the wash-off of dyes. Fabric color was analyzed by wash fastness, rubbing and crocking experiments. The combined treatment process was proven to be very effective in decolorizing both dyes by 99.1% and 96.15%. The color difference values of standard (dyed in fresh water) and batch samples for both dyes were 0.53 and 0.35, which is within the acceptable limit (≤1.0). The wash fastness test indicated a range of 4-5 for both standards and batch samples. In the case of water quality parameters, a decrease in pH values was observed after treatment; however, an increasing trend was seen in cases of COD, TDS and EC. While these parameters do not affect the color of the fabric in dying process, this study concludes that the combined treatment of chemical and electrocoagulation is very efficient for decolorizing dye wastewater. Reuse of treated wash-off wastewater is suitable not only for reducing water consumption but also reducing wastewater generation in the textile industry. Hence this treatment option will improve overall water efficiency of the textile industry.
Peach fruit and trees are prone to various issues, regarding yield, quality and pest attack. Calcium plays several roles in plant and fruit development. Therefore the current study was conducted to evaluate the response of peach fruit to foliar application. The experiment was laid out in Randomized Complete Block Design with two factors factorial arrangement and three replicates. Calcium chloride was applied at the rate of 0, 1, 2 and 3%. The foliar application of calcium was done at pink bud stage, berry size fruit stage, and then at pit hardening stage of peach. The obtained results indicated that foliar application of calcium significantly improved peach fruit quality and yield. The highest fruit weight (142.6 g), yield tree⁻¹ (15.6 kg), ascorbic acid content (6.67 mg∙100 g⁻¹), total soluble solids (11.0°Bx), fruit juice pH (3.98), sugar to acid ratio (45.1), fruit firmness (3.90 kg∙cm⁻²) and fruit volume (155.6 cm³) was obtained with the application of 3% solution of calcium chloride at pit hardening stage but it significantly reduced the percent titratable acidity (0.3%) and disease incidence (5.8%). It was concluded that 3% calcium chloride applied at the pit hardening stage significantly boosted peach quality and fruit yield.
The development of drought tolerant wheat cultivars has been slow due to lack of understanding the diagnostic physiological parameters associated with improved productivity under water stress. We evaluated responses to PEG induced osmotic stress under hydroponics in D-genome synthetic derived and bread wheat germplasm with the main aim to unravel and identify some promising attributes having role in stress tolerances. Genotypes used in this study differed in their morpho-physiological and biochemical attributes. Tolerant genotypes exhibited the ability to ameliorate harmful effects of PEG induced osmotic stress through better osmotic adjustment achieved through substantial relative water content (RWC), lowered osmotic potential, relatively stable root length having maximum water extraction capacity, significant increase in osmoprotectant concentration and relatively enhanced antioxidant activities. The results clearly revealed the importance of synthetic derivatives over check cultivars and conventional wheats in terms of osmotic stress responses. Interestingly, synthetic-derived advanced lines with Aegilops tauschii in its parentage including AWL-02, AWL-04 and AWL-07 proved superior over the best rainfed check cultivar (Wa-01). It was concluded that syntheticderived wheats has great potential to improve a range of stress adaptive traits. It could, therefore, be recommended to be a useful strategy for allowing modern bread wheat to become adapted to a wider range of environments in future climate change scenarios.
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