Wyniki wyszukiwania

1

Feasibility of sewage sludge leached by Aspergillus niger in land utilization

100%

Xu Y., Feng Y.-Y.

Polish Journal of Environmental Studies

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2016

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tom 25

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nr 1

Aspergillus niger is adopted in bioleaching to dispose of sludge from an urban wastewater treatment plant to determine the bioleaching influence of sludge on heavy metal fraction and nutritive material loss. The enrichment effect on wheat seedlings of Cu, Zn, Pb, and Cd in sludge is researched by a soil-cultivated experiment. Results indicate that the removal rates of Cu, Zn, Pb, and Cd are 74.1%, 87.6%, 82.1%, and 97.8% in the sludge after bioleaching, respectively. The remaining heavy metal in the sludge is mainly residual fraction followed by oxidizable fraction. The bioefficiency of the heavy metal decreases significantly. The loss rates of N, P, and K in the sludge are 29.8%, 13.3%, and 28.8%, respectively, with good fertility after bioleaching. The enrichment capacity order of heavy metals is Zn>Pb>Cu>Cd in wheat root and stem leaf, which is consistent with the order of unstable fraction content in the sludge after bioleaching.

2

Planning and policy of renewable energy utilization in a rural economic development zone

100%

Zhang C., Xu Y.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 5

3

MicroRNA-495 inhibits the high glucoseinduced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

81%

Wang X., Jin H., Jiang S., Xu Y.

Cellular and Molecular Biology Letters

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2018

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tom 23

Background: MicroRNAs (miRNAs) have physiological and pathophysiological functions that are involved in the regulation of cardiac fibrosis. This study aimed to investigate the effects of miR-495 on high glucose-induced cardiac fibrosis in human cardiac fibroblasts (CFs) and to establish the mechanism underlying these effects. Methods: Human CFs were transfected with an miR-495 inhibitor or mimic and incubated with high glucose. The levels of NOD1 and miR-495 were then determined via quantitative RT-PCR. Pro-inflammatory cytokine levels, cell differentiation and extracellular matrix accumulation were respectively detected using ELISA, quantitative RT-PCR and western blot assays. The luciferase reporter assay, quantitative RT-PCR and western blot were used to explore whether NOD1 was a target of miR-495. The effects of miR-495 on the NF-κB and TGF-β1/Smad signaling pathways were also detected via western blot. Results: Our results show that high glucose can significantly increase the expression of NOD1 in a time-dependent manner. Upregulation of miR-495 significantly alleviated the high glucose-induced increases in cell differentiation and collagen accumulation of CFs. Moreover, the bioinformatics analysis predicted that NOD1 was a potential target gene for miR-495. The luciferase reporter assay showed that miR-495 can directly target NOD1. The introduction of miR-495 could significantly inhibit the high glucose-activated NF-κB and TGF-β1/Smad signaling pathways. Conclusion: Upregulation of miR-495 ameliorates the high glucose-induced inflammatory, cell differentiation and extracellular matrix accumulation of human CFs by modulating both the NF-κB and TGF-β1/Smad signaling pathways through downregulation of NOD1 expression. These results provide further evidence for the protective effect of miR-495 overexpression in cases of high glucose-induced cardiac fibrosis.

4

Organizacja hodowli zwierzat futerkowych w Chinach

81%

Ilyukha V. A., Xu Y., Lapinski S.

Zwierzęta Futerkowe

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2015

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nr 10

5

Landfill leachate treatment using a combination of heterotrophic denitrification and partial nitritation in a single sequencing batch reactor

81%

Xu Y., Zhou S., Li H.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 1

6

Altitude variation in chilling tolerance among natural populations of Elymus nutans in the Tibetan Plateau

81%

Fu J., Xu Y., Miao Y., Hu T.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 09

7

Biology and chemistry of fumonisin and AAL toxins

81%

Mirocha C. J., Chen J., Xie W., Xu Y.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1993

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tom 37

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nr 1-2

8

Rapid non-empirical approaches for estimating relative binding free energies

81%

Mark A. E., Xu Y., Liu H., Van Gunsteren W. F.

Acta Biochimica Polonica

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1995

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tom 42

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nr 4

Rapid non-empirical methods for estimating binding free energies are reviewed. A novel approach based on the application of the free energy perturbation formula to a biased ensemble is presented. Preliminary results demonstrating the applicability of this approach in protein systems are shown and the potential of this method in structure-based drug design is discussed.

9

Comparative proteomic analysis of Cd-responsive proteins in wheat roots

71%

Wang Y., Qian Y., Hu H., Xu Y., Zhang H.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 2

Cadmium (Cd) is a major environmental toxicant to plant cells due to its potential inhibitory effects on many physiological processes. To gain a comprehensive understanding of plant response to Cd, wheat seedlings were exposed to a range of Cd concentrations (10, 100 and 200 µM) for 1 week and a combination of physiological and proteomic approaches were used to evidence Cd effects and to access the plant response to Cd toxicity. Root and shoot elongation was decreased, whereas the H₂O₂ and malondialdehyde content in wheat seedlings was increased significantly at higher Cd concentration. Protein profiles analyzed by two-dimensional electrophoresis revealed that 46 protein spots showed 1.5-fold change in protein abundance following Cd exposure; 31 protein spots were upregulated and 15 protein spots were down-regulated; 25 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. As expected, most of the up-regulated proteins are involved in heavy metal detoxification and antioxidant processes. Enzyme activity analysis revealed that ascorbate peroxidase and glutathione S-transferase activity was stimulated by Cd treatment. Abundance changes of these proteins, together with their putative functions provide us a new insight that can lead to an integrated understanding of the molecular basis of Cd responses in plants.

10

Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes

71%

Xu Y., Lu Y., Zhang R., Wang H., Liu O.

Acta Biochimica Polonica

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2016

|

tom 63

|

nr 2

11

Modeling the quality and quantity of runoff in a highly urbanized catchment using storm water management model

71%

Li C., Liu M., Hu Y., Gong J., Xu Y.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 4

As urbanization increases, urban runoff becomes an increasingly more important component of urban non-point pollution. In this study, the Storm Water Management Model was used to simulate the quantity and quality of runoff in a highly urbanized catchment. Data from three rainfall events were collected and used for model calibration and validation. Model performance was assessed using the Nash-Sutcliffe coefficient, relative error, and coefficient of determination. The modified Morris screening method was used for local sensitivity analysis. Sensitivity analysis results showed that the destore imperv and condit roughness parameters had the most influence on the hydrology and hydraulic module. Road exponent was the most sensitive parameter in determining TSS quantity and peak concentration. The calibration and verification results indicated that the model structure and parameters fitted the runoff-producing pattern. The total simulation accuracies of TSS, TN, TP, and COD loads, as assessed by the R2 value, were 0.82, 0.87, 0.72, and 0.94, respectively.

12

Transformation of wheat Triticum aestivum with the HvBADH1 transgene from hulless barley improves salinity-stress tolerance

61%

Li P., Cai J., Luo X., Chang T., Li J., Zhao Y., Xu Y.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 09

Studies have shown that the stress tolerance of cereal plants to osmotic or salinity stresses can be improved to varying degrees by the overexpression of an introduced betaine aldehyde dehydrogenase (BADH) gene. In the present study, the HvBADH1 gene from Hordeum vulgare L. var. nudum Hook. f., encoding a cytosolic BADH, was transferred into Triticum aestivum via traditional Agrobacterium tumefaciens-mediated transformation. Molecular methods, such as PCR, Southern blot analysis, and real-time quantitative RT-PCR were used to identify the successful integration and expression of the HvBADH1 transgene in genetically transformed wheat lines. To detect the efficacy of the HvBADH1 transgene in the transformants, some pivotal physiological indicators that reflected abiotic stress tolerance were measured in individual transgenic plant lines. These indicators included intracellular K⁺ and Na⁺ contents or K⁺/Na⁺ ratio, relative conductivity, and malondialdehyde and glycine betaine (GB) concentrations in cells. The results revealed that all the tested transgenic lines could significantly increase the recruitments of K⁺ in their cytosol than the wild-type seedlings. Similarly, 11.59- to 21.82-fold greater accumulation of GB, 2.11–2.56 times higher calli relative growth rates, and 26.2–29.1% seedling survival rates were found in transgenic lines under 150 mM NaCl stressed conditions. Our results demonstrated that by overexpressing the HvBADH1 transgene in genetically transformed wheat, the overall salt tolerance of the target plants was significantly increased, and the damaging effects of high salinity were significantly reduced.

13

Molecular cloning and expression analysis of a squalene synthase gene from a medicinal plant, Euphorbia pekinensis Rupr.

61%

Zheng Z., Cao X., Li C., Chen Y., Yuan B., Xu Y., Jiang J.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 10

Euphorbia pekinensis Rupr., which is also known as a medicinal plant, produces a large amount of alkaloids, phytosterols and triterpenes. In this study, we reported on the cDNA cloning and characterization of a novel squalene synthase (SQS) from E. pekinensis. Squalene synthase catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to produce squalene (SQ), the first committed precursor for sterol and triterpene biosynthesis. The full length cDNA named EpSQS (Genbank Accession Number JX509735) contained 1,614 bp with an open reading frame of 1,236 bp encoding a polypeptide of 411 amino acids. The deduced amino acid sequence of the EpSQS named EpSQS exhibited a high homology with other plant SQSs, and contained a single domain surrounded by helices. Phylogenetic analysis showed that EpSQS belonged to the plant SQS kingdom. Tissue expression analysis revealed that EpSQS expressed strongly in roots, weakly in stems and leaves, implying that EpSQS was a constitutive expression gene. The recombinant protein was expressed in Escherichia coli and detected by SDS-PAGE and western blot. The high performance liquid chromatography (HPLC) analysis showed that EpSQS could catalyze the reaction from farnesyl diphosphate (FPP) to squalene.

14

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Optimization of processing parameters for lettuce vacuum osmotic dehydration using response surface methodology

61%

Yuan Y., Tan L., Xu Y., Dong J., Zhao Y., Yuan Y.

Polish Journal of Food and Nutrition Sciences

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2018

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tom 68

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nr 1

In order to obtain the optimal technological parameters of lettuce vacuum osmotic dehydration, the effects of osmotic temperature, slice thickness, sucrose concentration, and vacuum degree on the vacuum osmotic dehydration were explored. The lettuce water loss rate and solid gain rate decreased with the increase of slice thickness and vacuum degree, and increased with the increase of sucrose concentration and osmotic temperature. Response surface methodology was applied to analyze the infl uence of the four infl uential factors on the evaluated parameters and the optimization of lettuce vacuum osmotic dehydration was studied. The results indicated that, within the experimental scope, the optimized technological parameters of lettuce vacuum osmotic dehydration are the temperature of 28º C, the slice thickness of 2 mm, sucrose concentration of 47%, the vacuum degree of 22 kPa, and the water loss rate and solid gain rate are 72.16% and 11.82%, respectively.

15

Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits

61%

Liu J., Guo S., He H., Zhang H., Gong G., Ren Y., Xu Y.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 11

Sugar content largely determines watermelon fruit quality. We compared changes in sugar accumulation and activities of carbohydrate enzymes in the flesh (central portion) and mesocarp of elite sweet watermelon line 97103 (Citrullus lanatus subsp. vulgaris) and exotic nonsweet line PI296341-FR (C. lanatus subsp. lanatus) to elucidate the physiological and biochemical mechanisms of sugar accumulation in watermelon fruit. The major translocated sugars, raffinose and stachyose, were more unloaded into sweet watermelon fruit than non-sweet fruit. During the fruit development, acid a-galactosidase activity was much higher in flesh of 97103 than in mesocarp of 97103, in flesh and mesocarp of PI296341-FR fruit. Insoluble acid invertase activity was higher in 97103 flesh than in 97103 mesocarp, PI296341-FR flesh or mesocarp from 18 days after pollination (DAP) to 34 DAP. Changes in soluble acid invertase activity in 97103 flesh were similar to those in PI296341-FR flesh and mesocarp from 18 DAP to full ripening. Sucrose synthase and sucrose phosphate synthase activities in 97103 flesh were significantly higher than those in 97103 mesocarp and PI296341- FR fruits from 18 to 34 DAP. Only insoluble acid invertase and sucrose phosphate synthase activities were significantly positively correlated with sucrose content in 97103 flesh. Therefore, phloem loading, distribution and metabolism of major translocated sugars, which are controlled by key sugar metabolism enzymes, determine fruit sugar accumulation in sweet and non-sweet watermelon and reflect the distribution diversity of translocated sugars between subspecies.

16

Genome-wide identification of the auxin response factor (ARF ) gene family and expression analysis of its role associated with pistil development in Japanese apricot (Prunus mume Sieb. et Zucc)

61%

Song J., Gao Z., Huo X., Sun H., Xu Y., Shi T., Ni Z.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 08

Auxin has been widely implicated in various aspects of plant growth and development, including flower development. In order to further elucidate the role of auxin during flower development, especially on the pistil development process, auxin response factors (ARFs), an important component in auxin signalling pathway, were studied in the early flower buds of Japanese apricot (Prunus mume Sieb. et Zucc). In this study, a comprehensive overview of the ARF gene family in Japanese apricot is presented, including the chromosomal locations, phylogenetic relationships, gene structures, the domain and nuclear localization analysis. Seventeen Japanese apricot genes that encode ARF proteins (PmARFs) have been identified based on the genome sequence of Japanese apricot. Comparison of the expression of some PmARF genes between perfect and imperfect flower buds in Japanese apricot suggests that PmARFs, especially the PmARF13 and PmARF17 gene may be required for pistil development and function in Japanese apricot. These results will be useful for future functional analyses of the ARF family genes in plants.

17

Morphological studies on five trachelocercids from the Yellow Sea coast of China, with a description of Tracheloraphis huangi spec. nov. (Ciliophora, Karyorelictea)

61%

Xu Y., Esaulov A., Lin X., Mazei Y., Hu X., Al-Rasheid K. A. S., Warren A.

Acta Protozoologica

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2011

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tom 50

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nr 3

18

Tracking the fate of fertilizer nitrogen in a paddy rice field using isotope technology

61%

Yu X., Xu Y., Zhu H., Shutes B., Yan B., Chen X., Cheng R.

Polish Journal of Environmental Studies

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2020

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tom 29

|

nr 1

19

Benefit allocation in the construction supply chain considering carbon emissions

61%

Du Q., Huang Y., Xu Y., Bai L., Bao T., Wang H.

Polish Journal of Environmental Studies

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2019

|

tom 28

|

nr 5

The construction industry has played an important role in reducing carbon emissions. Various policies have been implemented to stimulate construction enterprises to reduce carbon emissions, but the effects of emission reduction are not obvious, for they do not directly benefit the enterprises. This paper employs a modified Shapley value method to study benefit allocation in a construction supply chain considering carbon emissions. Four correction factors are proposed for modifying the initial allocation, namely the contribution rate of inputs, the risk-sharing coefficient, the degree of cooperation and the contribution rate of carbon emissions. We analyze carbon emissions based on an illustrative example of a concrete supply chain consisting of a cement manufacturer, a concrete manufacturer and a construction enterprise, and present our findings. First, the enterprises intend to cooperate to achieve the greatest benefit, and second, the benefit allocation is greatly affected by carbon emissions. Participants that produce more carbon emissions have higher carbon tax costs, which reduce profits. Further suggestions are also presented, which may help enterprises reduce carbon emissions. And policy makers should arrive at a suitable level of carbon tax to promote the smooth progress of projects and to improve the emission reduction effect.

20

Screening proteins interacting with VpPR10.1 of Chinese wild grapevine using the yeast two-hybrid system

61%

Xu T.-F., Xiang J., Li F.-J., Li T.-M., Yu Y.-H., Wang Y.-J., Xu Y.

Acta Physiologiae Plantarum

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2013

|

tom 35

|

nr 08

Among the 17 plant pathogenesis-related (PR) protein families, only PR10 family is intracellular and cytosolic. PR proteins are expressed in response to pathogen challenge and abiotic stresses in higher plants. However, the molecular mechanisms of their actions remain poorly understood. In a previous work, we isolated a PR10 gene from Erysiphe necator-resistant Chinese wild Vitis sp. (Baihe-35-1) and it was designated as VpPR10.1. In this study, yeast two-hybrid method was used to screen proteins interacting with VpPR10.1 proteins. Twenty-one ESTs were isolated and sequenced. All sequences were compared using BLASTx to identify presumptive orthologs. Several proteins associated with VpPR10.1 protein were screened, including CNR8, UFGT6, HSP, DEAD-box, Trx h2, Grx C9 and GLOX. These proteins are closely related to defensive action of plants against pathogens and abiotic stresses. Our results reveal that VpPR10.1 gene may be involved in hormone signaling, programmed cell death and defense responses of grapevine.

Ograniczanie wyników

Feasibility of sewage sludge leached by Aspergillus niger in land utilization

Planning and policy of renewable energy utilization in a rural economic development zone

MicroRNA-495 inhibits the high glucoseinduced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

Organizacja hodowli zwierzat futerkowych w Chinach

Landfill leachate treatment using a combination of heterotrophic denitrification and partial nitritation in a single sequencing batch reactor

Altitude variation in chilling tolerance among natural populations of Elymus nutans in the Tibetan Plateau

Biology and chemistry of fumonisin and AAL toxins

Rapid non-empirical approaches for estimating relative binding free energies

Comparative proteomic analysis of Cd-responsive proteins in wheat roots

Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes

Modeling the quality and quantity of runoff in a highly urbanized catchment using storm water management model

Transformation of wheat Triticum aestivum with the HvBADH1 transgene from hulless barley improves salinity-stress tolerance

Molecular cloning and expression analysis of a squalene synthase gene from a medicinal plant, Euphorbia pekinensis Rupr.

Optimization of processing parameters for lettuce vacuum osmotic dehydration using response surface methodology

Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits