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Linkage and association studies suggested the relationship between α-adducin polymorphism (Gly460Trp; rs4961) and genetic susceptibility to salt-sensitivity. However, the currently available results were inconsistent. This study aimed to define quantitatively the association between salt-sensitivity and α-adducin Gly460Trp polymorphism in all published case-control studies. Publications from PubMed and other databases were retrieved. The major inclusion criteria were: (1) case-control design; (2) salt-sensitivity confirmed by sodium loading tests, and (3) the distribution of genotypes given in detail. Seven case-control studies fulfilled the inclusion criteria. In total they involved 820 subjects (454 salt-sensitive and 366 non-salt-sensitive). The meta-analysis shows that Gly460Trp polymorphism in general is not significantly associated with salt-sensitivity [OR (95%CI): 1.40 (0.96,2.04), P = 0.08]. Subgroup analysis showed that the association is statistically significant in Asian people [OR (95%CI): 1.33 (1.06, 1.69), P = 0.02] but not in Caucasian people [OR (95%CI): 1.98 (0.57, 6.92), P = 0.28]. This indicates that blood pressure response to sodium varies between ethnical groups. More studies based on a larger population are required to evaluate further the role of α-adducin Gly460Trp polymorphism in salt-sensitive hypertension.
Phenotypic plasticity acts to increase the performance of plants under stress. Leaf morphological plasticity and its causes in different environments are incompletely understood. We measured the leaf morphological parameters of Quercus acutissima Carr. seedlings, including leaf size, leaf shape and venation pattern, assessing the effects of different habitat conditions on leaf morphological plasticity. A field study in forest edge and understory was combined with experiments simulating different light and water conditions. Leaf morphology variations occurred over most of the parameters, and the causes were consistent between the field study and lab experiment. Leaf size decreased with low supply of light and water. Leaf length and width were only affected by leaf area. The leaf petiole did not lengthen under shade stress, suggesting a trade-off relationship between functional tissues and support structures. Leaf shape became narrower in drought and broader in the shade, as reflected in changes in three leaf fractions. Higher vein density played a part in enhancement of mechanical support and water supply. Leaves with more teeth show more active photosynthesis, but are disadvantageous in xeric environments because of higher transpiration. Light was the main factor inducing leaf morphological plasticity. The variations caused by drought were due mainly to the allometry. Our results showed that the leaves of Q. acutissima seedlings respond to different habitats with phenotypic plasticity of morphology, suggesting that this is an important mechanism for seedlings to adapt to broader ecological amplitudes.
Ascorbic acid (Asc) plays a multifunctional role in plants. L-galactono-1,4-lactone dehydrogenase (GLDH, EC 1.3.2.3) catalyzes the last step in the main pathway of Asc biosynthesis in higher plants. In this paper, we first examined how a change in Asc content leads to a changed plant growth and seed set using GLDH transgenic rice (Oryza sativa L.) which has different expression level of GLDH. The results showed that suppression of GLDH expression resulted in a loss of chlorophyll, a lower Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) protein content, and a lower rate of CO₂ assimilation. As a consequence, a slower rate of plant growth and lower seed set were observed. Reduced seed set and growth rate as measured by plant height, root length, leaf weight, and root weight were consistent with the GLDH-mediated reduction of photosynthetic function. Increasing GLDH expression maintained high levels of chlorophyll, Rubisco protein, and a higher rate of net photosynthesis, resulting in higher seed set. The observation that increasing the level of GLDH expression correlated with reduced lipid peroxidation whereas reducing GLDH expression correlated with increased lipid peroxidation was consistent with the foliar level of Asc, indicating that GLDH functions to protect against ROSmediated damage. When taken together, this work suggests that level of Asc in transgenic rice for GLDH is associated with plant growth and seed set.
Water is a main factor limiting plant growth. Integrative responses of leaf traits and whole plant growth to drought will provide implications to vegetation restoration. This study investigated the drought responses of Vitex negundo L. var. heterophylla (Franch.) Rehd. with a focus on leaf morphology and physiology, seedling growth and biomass partitioning. Potted 1-year-old seedlings were subjected to four water supply regimes [75, 55, 35 and 15% field capacity (FC)], served as control, mild water stress, moderate water stress and severe water stress. Leaf morphological traits varied to reduce the distance of water transfer under water stress and leaflets were dispersed with drought. Net photosynthetic rate decreased significantly under water stress: stomatal closure was the dominant limitation at mild and moderate drought, while metabolic impairment was dominant at severe drought. The physiological impairment at severe drought could also be detected from the relative lower water use efficiency and nonphotochemical quenching to moderate water stress. Total biomass of well-watered plants was more than twice that at moderate water deficit and nearly ten times that at severe water deficit. In summary, V. negundo var. heterophylla had adaptation mechanism to water deficit even in the most serious condition, but different strategies were adopted. Seedlings invested more photosynthate to roots at mild and moderate drought while more photosynthate to leaves at severe drought. A nearly stagnant seedling growth and a sharp decline of total biomass were the survival strategy at severe water stress, which was not favorable to vegetation restoration. Water supply above 15% FC is recommended for the seedlings to vegetation restoration.
To test the feasibility of using field-collected scats as a source of DNA in the study of the least weasel Mustela nivalis nivalis Linnaeus, 1766, DNA was extracted from scat samples collected from captive weasels using a modified extraction protocol. Using universal primers, the control region of the mitochondrial genome was successfully amplified from scat-extracted DNA. This amplification resulted in two products; one equivalent in size and sequence to the product obtained from tissue-extracted weasel DNA, and the other slightly larger and equivalent in size and sequence to the domestic house mouse Mus musculus, the food source of the captive weasels. This demonstrates the reliability of DNA extraction from scats, as well as the possibility, under favourable circumstances, of identifying the prey species from the same samples. In addition, we attempted to amplify microsatellite loci from both tissue and scat-extracted DNA using six primer pairs designed for other mustelids, the American mink Mustela vison and the wolverine Gulo gulo. While three loci, Mvi57 (American mink), Ggu216 and Ggu234 (wolverine), were found to be polymorphic in the least weasel, amplification of these loci from the scat extracted DNA was only successful for approximately half of the samples. Although further work is needed, the present results suggest that it is possible to use scats as a source of DNA in field studies of the least weasel.
Increased nitrogen deposition and biological invasions may be two crucial consequences of global change. Exotic plants often have adverse effects on native plants. However, there are reports of occasional positive interaction between them. Increased nitrogen deposition enhances nitrogen availability in soil, which may facilitate the growth of some exotic plant and decrease the competitive advantage of native plants because of their adaptation to low-nutrient environments. Consequently, increased nitrogen deposition may regulate the relationship between exotic and native plants. A greenhouse experiment was, thus, conducted to examine the effects of increased nitrogen deposition on the relationship between the native Quercus acutissima and the exotic Robinia pseudoacacia, which are both widely distributed in North China. Seedlings of both species were grown in monoculture and mixture arrangements in a pot experiment with different nitrogen addition levels (i.e., 0, 2, 5, and 10 g N m–2 a–1). Nitrogen addition altered the biomass partitioning of Q. acutissima and R. pseudoacacia seedlings, with more biomass allocated to leaves and less to roots. Although R. pseudoacacia seedlings always had growth predominance over Q. acutissima seedlings, Q. acutissima was not threatened by R. pseudoacacia at the four levels of nitrogen addition during the three-month duration of the experiment. A positive interaction between the seedlings of R. pseudoacacia and Q. acutissima was found, and increased nitrogen deposition did not affect the positive interaction. Further studies are necessary to determine whether or not R. pseudoacacia is harmful to native ecosystems and should be limited for forestation in North China.
Groundwater is considered a major source of drinking water and its quality a basis for good population health. In order to identify groundwater hydro-chemical characteristics and pollution conditions in Songnen Plain, groundwater hydro-chemical characteristics and nitrate-nitrogen (NO3-N) spatial distribution characteristics and the health risks were analyzed. Results showed that groundwater hydro-chemical type was mainly HCO3-Ca, which was associated with the action of calcite and silicate mineral weathering dissolution. The over standards rate of NO3-N accounted for 50.8%, the coeffi cient of variation was 183.57% which was high spatial variability, the high-risk area accounted for 88.78% of the total study area, and the high-risk area covered the area with water quality of classes IV, V, and part of class III. The high-risk area is mainly distributed in the eastern high plains and in the central low plains, while the low-risk zone accounts for only 11.22% of the total area and is mainly distributed in the western alluvial plain with scattered distribution in other areas.
The relationships between species richness and driving factors might vary with taxa and spatial scale. For this study we used plant species data of eight groups from nature reserves in China, namely pteridophytes, gymnosperms, angiosperms, vascular plants, Chinese endemic species, rare and endangered plants, woody plants, and herbaceous plants, and eight putative environmental predictors to explore the relationships between plant species richness and environmental factors at two spatial scales (temperate region and national scale). Our data suggested that area, mean annual temperature, and mean annual precipitation were the determinants of variation in species richness within these eight groups in the temperate nature reserves of Shandong Province in China. The relationships between plant species richness and environmental factors were consistent in different groups. However, the relationships between species richness and mean annual temperature varied with spatial scale. In the temperate region, species richness was negatively correlated with mean annual temperature and positively correlated with mean annual precipitation, whereas on a national scale species richness was positively correlated with both mean annual temperature and mean annual precipitation. Our study confirmed that the relationships between plant species richness and environmental factors in nature reserves were consistent in the studied groups and varied with spatial scale.
Salicornia bigelovii is an unusually salt-tolerant plant species and is able to continue to photosynthesize and grow in saline conditions. There is an interest in understanding the mechanisms of this salt tolerance. In this study, S. bigelovii plants grown under 400 mM NaCl had higher growth and higher photosynthetic capacity than those not grown with salt. The intensities of the fluorescence emission peaks in the thylakoid membrane spectra in the salt treatment were higher than in the control. PSII and PSI peaks of the control were blue-shifted, indicating that salt deficiency affects energy transfer and structural stability in PSII and PSI. Both SDS-PAGE and western blot illustrated that the PSI proteins PsaA and PsaB and the PSII proteins CP47 and CP43 were upregulated with salt treatment, which might partly explain the change in the energy transfer process. Furthermore, Lhcb1 was also stimulated by salt treatment while Lhcb2 and Lhcb3 did not show noticeable changes. This demonstrated that salt is important in the maintenance of photosynthesis. In summary, treatment with salt led to an increased amount of PsaA/B, CP47, CP43, and Lhcb1 with a concurrent increase in antennae size. These photosystem changes may be responsible for the adaptation of S. bigelovii to saline conditions.
This study aimed to investigate plasticity of different plant traits to varied light and water availability. A greenhouse experiment was conducted with Quercus aliena seedlings with two light and four soil water levels. Plant traits related to leaf physiology, morphology, anatomy, and biomass production were determined. The results showed that plant size had significant effects on leaf area, leaf number, shoot height, basal diameter and crown area. After excluding the influence of plant size, water treatment had stronger effects on plants compared to light levels, and their interaction effect was significant. The limited water supply significantly inhibited leaf photosynthetic rate and the fluorescence efficiency under high light. However, leaves submitted to moderate drought stress showed enhanced fluorescence activity under shade condition. Grand plasticity of leaf physiology and growth was the highest, followed by biomass allocation and leaf morphology, and lastly anatomy, and this ranking did not change as resources considered. Among the variables, leaf petiole length, chlorophyll content and leaf area could be selected as candidates for estimation of species’ plasticity to water, light and their interaction, respectively. Therefore, our results suggested that there was a hierarchy existing among traits plasticity in Q. aliena, and supported the aboveground facilitation hypothesis that shade could alleviate the adverse effect of drought.
The particle of calcareous sands was forced to crush, then the energy from the crushing was released by the form of sound waves. Therefore the AE technique was used to detect the calcareous sands AE signal when it crushed. by to study the AE characteristics, the mechanics of calcareous sands was studied. Study showed that: (1) there was the AE activities on the low confining pressure condition at the beginnig of test, (2) there was more and more AE activities with the continuing of test until to the end, (3) the calcareous sands’ AE activities was on the whole testing, (4) the calcareous sands’ particle crushing and mutual friction played different roles for its AE activities. Then the AE model based on the calcarous sands’ particle crushing was discussed
The plasticity response of Quercus variabilis and Quercus mongolica seedlings to combined nitrogen (N) deposition and drought stress was evaluated, and their performance in natural niche overlaps was predicted. Seedlings in a greenhouse were exposed to four N deposition levels (0, 4, 8, and 20 g N m-2 year-1) and two water levels (80 and 50 % field-water capacity). Plant traits associated with growth, biomass production, leaf physiology, and morphology were determined. Results showed that drought stress inhibited seedling performance, altered leaf morphology, and decreased fluorescence parameters in both species. By contrast increased N supply had beneficial effects on the nutritional status and activity of the PSII complex. The two species showed similar responses to drought stress. Contrary to the effects in Q. mongolica, N deposition promoted leaf N concentration, PSII activity, leaf chlorophyll contents, and final growth of Q. variabilis under well-watered conditions. Thus, Q. variabilis was more sensitive to N deposition than Q. mongolica. However, excessive N supply (20 g N m-2 year-1) did not exert any positive effects on the two species. Among the observed plasticity of the plant traits, plant growth was the most plastic, and leaf morphology was the least plastic. Therefore, drought stress played a primary role at the whole-plant level, but N supply significantly alleviated the adverse effects of drought stress on plant physiology. A critical N deposition load around 20 g N m-2 year-1 may exist for oak seedlings, which may more adversely affect Q. variabilis than Q. mongolica.
Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan ≫ N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.
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