Wyniki wyszukiwania

1

Purification and characterization of oxalate oxidase from wheat seedlings

100%

Hu Y., Guo Z.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 2

Oxalate oxidase (OxO, EC 1.2.3.4.) was purified to homogeneity from wheat (Triticum aestivum) seedlings by sequential thermal treatment, ultrafiltration, Sephadex G-100 gel filtration and affinity chromatography with concanavalin A. The enzyme was purified 66.11-fold with a recovery of 21.97%. It showed a subunit molecular mass of 32.6 kDa on SDS-PAGE and a native molecular mass of 170 kDa on Sephadex G-150 filtration, suggesting that it is a pentamer. The wheat OxO had a maximum activity at pH 3.5. Its Km for oxalate was 0.21 mM. Chemical modification revealed that cysteine, lysine and carboxylate residues were essential for OxO activity, whereas arginine, serine, threonine and tryptophane residues were not essential.

2

Production of isoflavone genistein in transgenic IFS tobacco roots and its role in stimulating the developmnet of arbuscular mycorrhiza

81%

Tan Z., Liu R., Hu Y., Lin Z.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 5

Flavonoids and isoflavonoids are secondary metabolites in plants. With the goal of obtaining isoflavonoids from a wide range of plants, a few key studies have proven that isoflavonoids can be produced in non-leguminous plants by transgenic engineering. Many earlier studies investigate genistein biosynthesis in leaves and petals of isoflavone synthase (IFS) transgenic tobacco. However, most reports do not attempt to analyze quantification of genistein or do not check the presence of genistein in transgenic plant roots. In addition, little is known about the influence of genistein on arbuscular mycorrhiza (AM). In this paper, we reported that genistein was obtained from transgenic IFS tobacco roots. In addition, we revealed that endogenous genistein and 10 µg g⁻¹ exogenous genistein enhanced the development of AM symbiosis. We also revealed the relative expression levels of pertinent genes during the development of AM symbiosis. Our results suggest that genistein plays a positive role in the development of AM symbiosis in tobacco roots.

3

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A new tritylodontid from the Upper Jurassic of Xinjiang, China

81%

Hu Y., Meng J., Clark J. M.

Acta Palaeontologica Polonica

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2009

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tom 54

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nr 3

385-391

A new genus and species of Tritylodontidae, Yuanotherium minor, is described and compared with other known tritylodontids. The new taxon is represented by a partially preserved upper jaw with three postcanines, collected from the upper part of the Shishugou Formation (Oxfordian, Late Jurassic) in the Wucaiwan area of the Junggar Basin, northwestern Xinjiang, China. Like other tritylodontids its maxillary teeth have three rows of blade−like trenchant cusps separated by deep furrows. The new species differs from other tritylodontids mainly in having posteriormost two cusps of the median row on upper postcanines closely placed. The new tritylodontid may have been omnivorous rather than herbivorous, as previously suggested for tritylodontids in general.

4

Positive grass diversity-productivity relationships and controlling factors along an elevation gradient in Central Asian Alpine grasslands

81%

Li K., Fangmeier A., Hu Y., Liu X.

Polish Journal of Ecology

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2012

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tom 60

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nr 1

The productivity-diversity relationship in grasslands is of great interest with regard to species loss in natural ecosystems, where species extinction is not random but directed. We carried out a three-year investigation from 2005 to 2007 along an elevation gradient in alpine grasslands of Central Asia in an area of 70 x 20 km2 at Bayinbuluk, Tianshan Mountains. We selected 9 sites (10 x 10 m2) and 117 plots (1 x 1 m2) from 2460 to 3260 m a.s.l. with 100 m intervals of altitude. Species richness, productivity, soil characteristics, air temperature and relative humidity (May–August) were recorded. The relationship between plant diversity and productivity was significant and positive. We used ordination techniques such as Canonical Correspondence Analysis (CCA) to examine the relationship between vegetation and the environmental factors. The results showed that plant species composition, species richness and productivity were significantly affected by air temperature, soil pH and relative humidity across the study area.

5

Microstructure evolution and mechanical properties of underwater dry welded metal of high strength steel Q690E under different water depths

81%

Sun K., Hu Y., Shi Y., Liao B.

Polish Maritime Research

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2020

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tom 27

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nr 4

6

New dinosaur egg material from Yunxian, Hubei Province, China resolves the classification of dendroolithid eggs

81%

Zhang S., Yang T.-R., Li Z., Hu Y.

Acta Palaeontologica Polonica

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2018

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tom 63

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nr 4

The oofamily Dendroolithidae is a distinct group of dinosaur eggs reported from China and Mongolia, which is characterized by branched eggshell units and irregular pore canals. The ootaxonomic inferences, however, were rarely discussed until now. A colonial nesting site was recently uncovered from the Qinglongshan region, Yunxian, Hubei Province, China. More than 30 dendroolithid egg clutches outcrop on the Tumiaoling Hill, including an extremely gigantic clutch containing 77 eggs. All clutches were exposed in the Upper Cretaceous fluvial-deposited Gaogou For mation. In this study, we emend the diagnosis of the oogenus Placoolithus and assign all dendroolithid eggs from the Tumiaoling Hill to a newly emended oospecies Placoolithus tumiaolingensis that shows greatly variable eggshell microstructure. Moreover, our study also disentangles the previous vexing classification of dendroolithid eggs. We conclude that Dendroolithus tumiaolingensis, D. hongzhaiziensis, and Paradendroolithus qinglongshanensis, all of which were previously reported from Yunxian, should be assigned to the newly emended oospecies Placoolithus tumiaolingensis.

7

Pleistocene Equid Brain Endocast from Shanxi Province, China

81%

Hu Y., Chen Y., Wang S., Sun Q.

Acta Palaeontologica Polonica

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2014

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tom 59

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nr 2

Brain endocasts are rare in the fossil record because they are only preserved under exceptional conditions. An equid brain endocast from the early Pleistocene of Wanrong County, Shanxi Province, China, is reported in this paper. Measuring approximately 140 × 95.2 × 83 mm, the new specimen represents a relatively advanced adult horse brain. Comparisons indicate that it is more derived than those of Hyracotherium and Mesohippus in having an expanded neocortex, and more than those of Pliohippus and Hipparion in having an enlarged network of branching sulci; in most characters involving these sulci, the Shanxi brain conforms to the extant species Equus caballus. The sulcus diagonalis of the Equus brain appears to have evolved conservatively during the early Pleistocene, whereas the sulcus suprasylvius seems to have evolved rapidly. The specimen demonstrates that the development of a high degree of complexity predates the enlargement of the brain in the horse, which increased in length, breadth, and especially height during the late Cenozoic.

8

Expression of SYMRK affects the development of arbuscular mycorrhiza in tobacco roots

81%

Tan Z., Hu Y., Lin Z.

Acta Physiologiae Plantarum

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2013

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tom 35

|

nr 01

SYMRK is a plant receptor-like kinase with a role in root endosymbiosis. Heterologous expression of SYMRK from non-legumes can complement the loss-offunction effects of the mutant symrk in legumes. However, it is unclear whether the development of arbuscular mycorrhiza (AM) is affected along with the enhanced expression of SYMRK. In the present study, the full-length LsSYMRK gene was cloned from Lathyrus sativus. Overexpression of LsSYMRK in tobacco roots was essential for AM development, and affected the expression of genes which are involved in the potential signaling pathway of AM. In addition, the pattern of hyphal penetration changed from radial to longitudinal when hyphae reached the root cortex. Furthermore, overexpression of LsSYMRK increased tobacco biomass in the presence of AM fungi. These results suggest that increased expression of SYMRK in roots of AM-infected tobacco can increase the colonization and biomass.

9

Simulation model and state analysis of ship transmission line

81%

Hu Y., Xu M., Li Y.

Polish Maritime Research

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2018

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tom 25

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nr Special Issue S3

n order to discuss the simulation model of the ship transmission line and the state of the transmission line, an early fault model is built according to the evolution principle of the short circuit fault of the transmission line and combining with the fault characteristics of the early fault. A small distributed ship transmission line system is built in MATLAB/Simulink. Then, combined with the constructed fault module, the original short circuit module, and the load module, the various states (normal state, early fault state, severe early fault state, short circuit state) of the ship transmission line are stimulated, and the features of voltage signal in each state is analysed. It is concluded that, due to the normal operation of the ship transmission line system, the variation characteristics of the flow signal and voltage signal caused by the sudden load mutation, that is, the sudden load and the sudden increase load, are very similar to the changes caused by the early fault. Therefore, in order to find a more accurate early fault detection method, the state is divided into normal state, sudden load state, sudden increase and sudden decrease load state

10

Light restored root growth of Arabidopsis with constitutive ethylene response

81%

Hu Y., Chang C., Xu G., Wang T.

Acta Physiologiae Plantarum

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2011

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tom 33

|

nr 3

Ethylene response factor (ERF) is an important component in ethylene or pathogen-induced defensive response of plants. However, physiological effects of ERF on plants have not been fully elucidated. We previously identified an ERF gene, OsERF1, in rice. It up-regulated ethylene-responsive genes expression and influenced growth and development of the transgenic Arabidopsis. Here, we report that similar to other seedlings with constitutive ethylene response, OsERF1 seedlings were suppressed in their root growth. Interestingly, the suppressed root growth was restorable by light irradiation. Detailed analysis showed that OsERF1 inhibited cell elongation without influencing cell number in hypocotyls and leaves of the transgenic Arabidopsis. In addition, homozygous OsERF1 was fatal and heterozygous OsERF1 was harmful in Arabidopsis. These findings expand our understanding of ERF.

11

Docking strategies: binding of substrate and inhibitors to S-adenoslyhomocysteine hydrolase - a case study

81%

Kuczera K., Hu Y., Yin D., Borchardt R. T.

Cellular and Molecular Biology Letters

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2001

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tom 06

|

nr 2B

536-537

12

Spatial and temporal gene expression during seed germination of Brassica napus

81%

Ge F., Hu Y., Wang J.

Acta Physiologiae Plantarum

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2013

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tom 35

|

nr 10

A cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach was used to analyze the spatio-temporal transcript profilings in cotyledons, radicle and hypocotyl of Brassica napus (B. napus) seed during different stages of germination. In total, 75 differentially expressed transcript-derived fragments (TDFs) in cotyledons, 145 in radicle and 145 in hypocotyl were sequenced. Base on BLAST analyses, 37 TDFs in cotyledons, 66 in radicle and 72 in hypocotyl were homologous to genes with known function in the database, respectively. The above known transcripts (175 in total) in three tissues were classified into categories based on their biochemical functions. These genes belong to 14 different functional categories and most of them correspond to metabolic processes, followed by transcription, signal transduction, translation and transport. However, many classes were expressed specifically, including regulation of metabolism and protein function genes in cotyledons, cell rescue and defense genes in radicle, signal transduction genes in hypocotyl. Besides, the expression of eleven identified genes was verified by semi-quantitative reverse transcription polymerase chain reaction in different tissues. The results showed differential gene expression at various germination stages and characteristic gene expression in different seed tissues. Analysis of gene expression profile of B. napus seed is useful for further investigating the seed germination mechanism.

13

Monobutyl phthalate induces the expression change of G-protein-coupled receptor 30 in rat testicular Sertoli cells

81%

Hu Y., Li D., Lu Y., Han X.

Folia Histochemica et Cytobiologica

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2013

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tom 51

|

nr 1

14

Water salinity and initial development of four guava [Psidium guajava L.] cultivars in North-Eastern Brazil

81%

Cavalcante I. H. L., Cavalcante L. F., Hu Y., Beckmann-Cavalcante M. Z.

Journal of Fruit and Ornamental Plant Research

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2007

|

tom 15

15

Estimation of wind erosion rates of Mongolian Plateau by using 137Cs tracing technique

71%

Qi Y., Liu J., Shi H., Zhuang D., Hu Y.

Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation

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2010

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tom 42

|

nr 1

197-208

Wind erosion is one of the major environmental problems in semi-arid and arid regions. Here we established a transect from northwest (Tariat, Mongolia) to southeast (Xilingol, Inner Mongolia of China) across the Mongolian Plateau, and selected eight sampling sites along the transect. We then estimated the soil wind erosion rates by using the 137Cs tracing technique and examined their spatial dynamics. In the Mongolia section (from Tariat to Sainshand), the wind erosion rate increased gradually with vegetation type and climatic regimes; the wind erosion process was controlled by physical factors such as annual precipitation and vegetation coverage, etc. While in the China section (Inner Mongolia), the wind erosion rates of Xilinhot, Zhengxiangbai Banner and Taipusi Banner were thrice as much as those of Bayannur of Mongolia, although these four sites were all dominated by typical steppe. Besides the physical factors, higher population density and livestock carrying level should be responsible for the higher wind erosion rates in these regions of Inner Mongolia.

16

Beneficial effects of silicon in alleviating salinity stress of tomato seedlings grown under sand culture

71%

Li H., Zhu Y., Hu Y., Han W., Gong H.

Acta Physiologiae Plantarum

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2015

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tom 37

|

nr 04

Sand culture experiments were designed to investigate the effect and mechanism of exogenous silicon on salt tolerance of tomato plants. Plant growth, photosynthetic gas exchange, water status, ion accumulation, root morphological traits, root water uptake and antioxidant defense were analyzed under 150 mM NaCl without or with application of 2 mM silicon. Application of silicon improved tomato growth, photosynthetic pigment and soluble protein contents, net photosynthetic rate and root morphological traits under salt stress. The leaf transpiration rate and stomatal conductance were not decreased, but increased by application of silicon under salt stress. Meanwhile, silicon decreased the concentrations of Na and Cl in the roots, stem and leaves, but without any sign of decrease in the root-to-shoot translocations. Leaf water status, root hydraulic conductance and antioxidant ability were all improved by exogenous silicon under salt stress. Results suggest that silicon application could decrease Na and Cl accumulation and increase antioxidant defense in tomato roots, which improved the root growth and hydraulic conductance, and therefore improved leaf water status and shoot growth. The study also suggests a potential value of silicon application in soilless culture (sand culture) in tomato production.

17

Nitrogen-fixing bacterial communities exhibit strong spatial structure along a slope gradient in karst shrublands ecosystem

71%

Liang Y., He X., Chen X., Hu Y., Su Y.

Polish Journal of Environmental Studies

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2019

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tom 28

|

nr 5

Environmental heterogeneity is a primary factor in determining soil microbial spatial patterns. However, few studies have demonstrated a link between environmental heterogeneity and the spatial patterns in free-living nitrogen-fixing bacteria, which are important in nitrogen cycling. Here, quantitative polymerase chain reaction and terminal restriction fragment length polymorphism were used to determine the spatial distribution of free-living nitrogen-fixing bacteria along a slope gradient. Plant communities were evaluated through field surveys, and basic soil properties were measured in the laboratory. Soil properties (e.g., soil available phosphorus, soil organic carbon, and total nitrogen) were higher in upper- and middle -slope soils than in lower-slope soils, while bacterial abundance was the opposite. Bacterial and plant communities all varied along the slope gradient. Redundancy analysis revealed that bacterial community composition was closely linked to pH, soil organic carbon, available phosphorus, and total nitrogen. These results indicate that free-living nitrogen-fixing bacterial communities show strong spatial structure along a slope gradient, and emphasize the importance of soil heterogeneity in affecting bacterial spatial patterns in a karst region.

18

Antiviral and immunoregulatory role against PCV2 in vivo of Chinese herbal medicinal ingredients

71%

Yang H., Chen X., Jiang C., He K., Hu Y.

Journal of Veterinary Research

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2017

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tom 61

|

nr 4

19

Modeling the quality and quantity of runoff in a highly urbanized catchment using storm water management model

71%

Li C., Liu M., Hu Y., Gong J., Xu Y.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 4

As urbanization increases, urban runoff becomes an increasingly more important component of urban non-point pollution. In this study, the Storm Water Management Model was used to simulate the quantity and quality of runoff in a highly urbanized catchment. Data from three rainfall events were collected and used for model calibration and validation. Model performance was assessed using the Nash-Sutcliffe coefficient, relative error, and coefficient of determination. The modified Morris screening method was used for local sensitivity analysis. Sensitivity analysis results showed that the destore imperv and condit roughness parameters had the most influence on the hydrology and hydraulic module. Road exponent was the most sensitive parameter in determining TSS quantity and peak concentration. The calibration and verification results indicated that the model structure and parameters fitted the runoff-producing pattern. The total simulation accuracies of TSS, TN, TP, and COD loads, as assessed by the R2 value, were 0.82, 0.87, 0.72, and 0.94, respectively.

20

Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53

71%

Li T., Zhang Z., Hu Y., Duan X., Xin Z.

Journal of Applied Genetics

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2011

|

tom 52

|

nr 2

Powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) is an economically important disease in wheat worldwide. The identification of germplasms resistant to the disease can not only facilitate the breeding of resistant cultivars, but can also broaden the diversity of resistance genes. The Mexican M53 is a synthetic hexaploid wheat line developed at the International Maize and Wheat Improvement Center (CIMMYT) from the cross between Triticum durum and Aegilops tauschii249. Infection of M53 with 15 different pathogen races revealed that the resistance in M53 was race-dependent and effective against the majority of the tested Bgt races, including the race 15 predominant in the Beijing wheat growing area. Inoculation of the parents of M53 with the race 15 demonstrated that M53 and Ae. tauschii249 were resistant, whereas T. durum was susceptible. The inoculation of three segregating F2 populations developed from the crosses between M53 and three susceptible Chinese wheat cultivars with the race 15 showed that the resistant gene in M53 segregated in a single dominant manner. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were used to map the gene in a segregating F2 population consisting of 213 lines developed from the cross Wan7107 × M53. Two closely linked AFLP markers, Apm109 and Apm161, were identified to flank the gene with genetic distances of 1.0 cM and 3.0 cM, respectively. The recognized gene was assigned to the long arm of chromosome 5D as determined by three linked SSR markers, Xwmc289b, Xgwm583, and Xgwm292, and by the physical mapping of Apm109 using Chinese Spring nullisomic–tetrasomic and ditelosomic stocks. The resistance gene identified in M53, temporarily designated as Pm-M53, could be used in local wheat-breeding programs to improve powdery mildew resistance.

Ograniczanie wyników

Purification and characterization of oxalate oxidase from wheat seedlings

Production of isoflavone genistein in transgenic IFS tobacco roots and its role in stimulating the developmnet of arbuscular mycorrhiza

A new tritylodontid from the Upper Jurassic of Xinjiang, China

Positive grass diversity-productivity relationships and controlling factors along an elevation gradient in Central Asian Alpine grasslands

Microstructure evolution and mechanical properties of underwater dry welded metal of high strength steel Q690E under different water depths

New dinosaur egg material from Yunxian, Hubei Province, China resolves the classification of dendroolithid eggs

Pleistocene Equid Brain Endocast from Shanxi Province, China

Expression of SYMRK affects the development of arbuscular mycorrhiza in tobacco roots

Simulation model and state analysis of ship transmission line

Light restored root growth of Arabidopsis with constitutive ethylene response

Docking strategies: binding of substrate and inhibitors to S-adenoslyhomocysteine hydrolase - a case study

Spatial and temporal gene expression during seed germination of Brassica napus

Monobutyl phthalate induces the expression change of G-protein-coupled receptor 30 in rat testicular Sertoli cells

Water salinity and initial development of four guava [Psidium guajava L.] cultivars in North-Eastern Brazil