Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Mechanizmy toksyczności i detoksykacji amoniaku u zwierząt przeżuwających żywionych mocznikiem

100%

Motyl T.

Postępy Nauk Rolniczych

|

1981

|

tom 28

|

nr 3

2

Uwalnianie amoniaku z białka przyczyną zawyżania wyników oznaczeń mocznika we krwi metodą Conwaya

100%

Motyl T.

Diagnostyka Laboratoryjna

|

1975

|

tom 11

|

nr 4

3

Kwas orotowy w mleku: pochodzenie, fizjologizcne znaczenie oraz konsekwencje spożycia

100%

Motyl T.

Medycyna Weterynaryjna

|

1990

|

tom 46

|

nr 05

4

Toksyczność amoniaku u owiec przy ciągłym i ograniczonym dostępie do paszy o dużej zawartości moczniku

100%

Motyl T.

Zeszyty Naukowe Akademii Rolniczo-Technicznej w Olsztynie. Weterynaria

|

1978

|

nr 10(188)

5

Acyduria orotowa u ludzi i zwierząt

100%

Motyl T.

Medycyna Weterynaryjna

|

1990

|

tom 46

|

nr 01-03

6

Apoptoza - smierc warunkujaca zycie

75%

Motyl T.

Postępy Biologii Komórki

|

1998

|

tom 25

|

nr 3

315-334

7

Mikromacierze DNA - nowe narzedzie badawcze w fizjologii i patologii

63%

Sadkowski T., Motyl T.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 11 Supl.

1421-1426

The aim of this review is to explain fundamentals of DNA microarray technique and indicate possibilities of its use in veterinary sciences. DNA microarray technology is a powerful tool for functional and structural genomics. It allows the analysis of an entire genome expression (transcriptomic profile) on one glass or plastic slide in a short period of time. It gives the opportunity to establish genes expression patterns characteristic for different physiological and pathological conditions and allows its use as a diagnostic tool. The use of expression microarrays gives the possibility to examine the influence of new treatment strategies in particular diseases and enables the assessment of advantages and disadvantages of this type of therapy. It also allows finding new targets (key proteins, enzymes) for newly developed drugs and makes possible the adaptation of a drug therapy to individual patients. By using DNA microarray technique it is possible to make a new hypothesis and to validate current ones. In this review the origin of the DNA microarray technique, its molecular basics, different kinds of microarray slides and analysis platform as well as the methodology of labeling and hybridization were described. This review may be very useful for people who are planning to use or are have already begun using DNA microarray technique.

8

Exploiting cancer genomics in pet animals to gain advantage for personalized medicine decisions

63%

Krol M., Motyl T.

Journal of Applied Genetics

|

2014

|

tom 55

|

nr 3

9

Apoptosis and autophagy in involuting bovine mammary gland

63%

Zarzynska J., Motyl T.

Journal of Physiology and Pharmacology. Supplement

|

2008

|

tom 59

|

nr 9

275-288

10

Zastosowanie technik hodowli przestrzennych [3D] w badaniach biologii gruczolu mlekowego bydla

63%

Kozlowski M., Motyl T.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 11 Supl.

1417-1420

The mammary gland has been extensively studied for over a century. However, the major contribution to our current knowledge of mammary gland biology has been made mostly by studying the mammary gland of humans and rodents, while ruminant models have lagged behind. During the last years, a variety of in vitro approaches have been employed to understand the regulation of mammary gland growth and morphogenesis during different developmental and physiological stages. There are only a few immortalized cell lines of bovine mammary gland epithelium. Unfortunately only three of them are IGF and EGF sensitive, which is very important in mammary gland development in vivo. Monolayer culture models are easy and convenient to set up but they lack the three-dimensional microenvironment of intact tissue. This review describes the three-dimensional (3-D) cell culture system, which has recently been widely used. Bovine primary mammary epithelium cell cultures and some immortalized cell lines plated on an extracellular matrix form functional acini-like structures. This model mimics organization and differentiation of an intact tissue and can be very useful in the investigation of bovine mammary gland biology.

11

Autofagia - narzedzie przezycia czy smierci komorki nowotworowej

63%

Lamparska-Przybysz M., Motyl T.

Postępy Biologii Komórki

|

2005

|

tom 32

|

nr 1

13-22

12

Molekularny mechanizm inwolucji gruczolu mlekowego krowy

63%

Zarzynska J., Motyl T.

Medycyna Weterynaryjna

|

2005

|

tom 61

|

nr 10

1110-1113

13

Dissimilar effects of LY 294002 and PD 098059 in IGF-I- mediated inhibition of TGF-beta1 expression and apoptosis in bovine mammary epithelial cells

63%

Zarzynska J., Motyl T.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 3

181-193

In mammary epithelial cells (MEC) TGF-ß1 is the auto-/paracrine growth inhibitor and inducer of apoptosis and therefore is considered as an important local regulator of mammary tissue involution. However, the mechanisms of controlled TGF-ß1 expression in the course of bovine mammary gland remodelling are still unclear. Recent study performed in this laboratory support the evidence that TGF-ß1 expression in bovine MEC is regulated by hormones of somatotropic axis (GH, IGF-I and somatostatin). Present study was focused on the contribution of IGF-I-induced signaling pathways in anti-TGF-ß1 and anti-apoptotic effects of IGF-I. Laser scanning cytometry was applied for the measurement of TGF-ß1 content and apoptotic cell number in bovine BME-UV1 MEC. Involution of the bovine mammary gland in vitro was modeled by decreasing the availability of FBS for bovine MEC. Reducing FBS content in the medium from 10% to 0.5% evoked highly significant increase of TGF-ß1 expression and increase of apoptotic cell number. IGF-I (50 ng/ml) completely abrogated FBS deficiency-induced TGF-ß1 expression and apoptosis in bovine MEC. In order to establish which of the IGF-I signaling pathways contributed to anti-TGF-ß1 and anti-apoptotic effects, the inhibitors of PI3-kinase - (LY 294002) and MEK- (MAPKK for ERK) (PD 098059) mediated signaling pathways were applied to our model. The results clearly showed that inhibition of PI3-K reverses the ability of IGF-I to suppress TGF-ß1 expression and apoptosis. An inhibition of ERK1/2 pathway even potentiated inhibitory effect of IGF-I on TGF-ß1 expression, but partially abrogated anti-apoptotic effect of IGF-I. In conclusion, the results of the study indicate that PI3-K/Akt pathway contributed significantly to the inhibition of TGF-ß1 expression by IGF-I, whereas both PI3-K/Akt and ERK1/2 pathways are involved in the anti-apoptotic effect of IGF-I in bovine MEC.

14

ALCAM-CD166 [Activated Leukocyte Cell Adhesion Molecule] - adhezyjna czasteczka regulujaca wzrost, rozwoj, agregacje oraz migracje komorek zdrowych i nowotworowych

63%

Jezierska A., Motyl T.

Postępy Biologii Komórki

|

2004

|

tom 31

|

nr 4

663-680

15

Związki pirymidynowe i purynowe w osoczu krwi, moczu i mleku krów zdrowych i chorych na enzootyczną białaczkę

63%

Motyl T., Jasinski J.

Medycyna Weterynaryjna

|

1989

|

tom 45

|

nr 05

Исследования провели на 56 коровах нч-п породы: здоровых и показывающих положительную серологическую реакцию на антиген вируса лейкемии, в том 4 коров с отчетливым лейкоцитозом (35,1 ± 9,3 тыс. лейкоцитов в мм³). Сравнивали уровень мочевой кислоты, цитозина, псевдоуридина, цитидина, гипоксантина, ксантина и уродина в кровяной плазме, оротовой кислоты и псевдоуридина в моче, а также оротовой кислоты в молоке исследуемых коров. Коровы, больные энзоотической лейкемией, показывали повышенный уровень псевдоуридина в кровяной плазме и моче, а также большую концентрацию оротовой кислоты в молоке по сравнению со здоровыми коровами. На метаболический эфдект лейкемии налагалось влияние факторов кормления, так как кормление увеличивало уровень пиримидиновых и пуриновых соединений в кровяной плазме и моче.

16

Autofagia w przebudowie gruczolu mlekowego bydla

51%

Gajewska M., Gajkowska B., Sobolewska A., Motyl T.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 11 Supl.

1412-1416

Autophagy is a process responsible for the degradation and recycling of cytoplasmic content by lysosomes. It is thought to facilitate cell survival during periods of nutrient starvation, but it can also be involved in other physiological processes including regulation of protein homeostasis, degrading intracellular bacteria, tumor suppression and regulation of programmed cell death. Our group was the first to report autophagy in bovine mammary epithelial cells, both in vitro, on BME-UV1 mammary epithelial cell line, and in vivo. The highest intensity of autophagy in bovine mammary glands is noted during dry periods, when the gland undergoes intensive involution and the deprivation of bioactive compounds (hormones, growth factors, cytokines) and nutrients occur. Our studies on the regulation of autophagy show that the conditions observed during bovine mammary gland involution, such as: 1) decrease in the release of lactogenic hormones and growth factors (prolactin, GH and IGF-I); 2) increase of apoptogenic factors (i.e. TGF-β₁) and their receptors, 3) increased synthesis of sex steroids (17-β estradiol, progesterone); and 4) the enhanced competition of intensively developing fetus and mother organism for nutritional and bioactive compounds, may create a state of temporary malnutrition of mammary epithelial cells that forces the cells to the induction of autophagy as a mechanism stabilizing intracellular supplies of energy and aminoacids, especially during the enhanced activity of apoptogenic factors.

17

Transcriptional pattern of TGF-beta1 inhibitory effect on mouse C2C12 myoblasts differentiation

51%

Wicik Z., Sadkowski T., Jank M., Motyl T.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 4

The aim of the present study was to define the effect of TGF-β1 on C2C12 myoblasts myogenesis. TGF-β1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-β1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-β1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-β1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-β1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-β1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Bax, MdfI, Tef, Tubg1, Cxcl5, Rho, Calca and Lgals4.

18

Rola komórek satelitowych we wzroście i regeneracji mięśni szkieletowych

51%

Ciecierska A., Sadkowski T., Motyl T.

Medycyna Weterynaryjna

|

2019

|

tom 75

|

nr 12

Postnatal growth and regeneration capacity of skeletal muscles is dependent mainly on adult muscle stem cells called satellite cells. Satellite cells are quiescent mononucleated cells that are normally located outside the sarcolemma within the basal lamina of the muscle fiber. Their activation, which results from injury, is manifested by mobilization, proliferation, differentiation and, ultimately, fusion into new muscle fibers. The satellite cell pool is responsible for the remarkable regenerative capacity of skeletal muscles. Moreover, these cells are capable of self-renewal and can give rise to myogenic progeny.

19

Trojwymiarowe hodowle komorek nablonka gruczolu sutkowego jako model badawczy procesu roznicowania

51%

Gajewska M., Kozlowski M., Motyl T.

Postępy Biologii Komórki

|

2010

|

tom 37

|

nr 1

121-135

20

Morfologiczne i molekularne wskazniki komorek macierzystych i progenitorowych w gruczole sutkowym

51%

Kolek O., Gajkowska B., Motyl T.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 02

136-141

The mammary gland is a dynamic organ that undergoes profound remodeling dependent on proliferation, differentiation, and apoptosis of mammary epithelial cells (MEC) during the cycle of the pregnancy, lactation, and involution. Long-lived populations of stem cells, which have a unique capacity for self-renewal, are responsible for these developmental changes. There is an increasing body of discoveries regarding human and mouse mammary gland stem cells, but the studies on bovine mammary gland stem cells are still very limited. According to morphological criteria bovine MEC are classified into two types: undifferentiated type I stem/progenitor cells assembling small light cells (SLC) and large light cells (LLC), and type II partially differentiated large dark cells (LDC) and terminally differentiated cells. To date there are no identified reliable molecular markers of stem/progenitor cells in bovine mammary glands. The main candidates are membrane transporting proteins of the Adenosine Binding Cassette (ABC) family, including Multi-drug-resistance protein 1 (Mdr1) and Breast cancer resistance protein 1 (Bcrp1). These proteins allow for the isolation of side populations (SP) of MEC assembling stem/progenitor cells by exclusion of dyes. Cytometric analysis of SP revealed from 0.2% to 5% of MEC in human and mouse mammary glands. The knowledge on the number and molecular properties of stem cells in bovine mammary glands would be very useful not only for enhancing milk production but also for explanation of the natural resistance against mammary cancer in this species.

Ograniczanie wyników

Mechanizmy toksyczności i detoksykacji amoniaku u zwierząt przeżuwających żywionych mocznikiem

Uwalnianie amoniaku z białka przyczyną zawyżania wyników oznaczeń mocznika we krwi metodą Conwaya

Kwas orotowy w mleku: pochodzenie, fizjologizcne znaczenie oraz konsekwencje spożycia

Toksyczność amoniaku u owiec przy ciągłym i ograniczonym dostępie do paszy o dużej zawartości moczniku

Acyduria orotowa u ludzi i zwierząt

Apoptoza - smierc warunkujaca zycie

Mikromacierze DNA - nowe narzedzie badawcze w fizjologii i patologii

Exploiting cancer genomics in pet animals to gain advantage for personalized medicine decisions

Apoptosis and autophagy in involuting bovine mammary gland

Zastosowanie technik hodowli przestrzennych [3D] w badaniach biologii gruczolu mlekowego bydla