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1

Syntetaza tlenku azotu w jadrach przedwzrokowych, nadwzrokowych i guzowych podwzgorza swini - dystrybucja i kolokalizacja z DBH, LHRH i receptorem estrogenowym

100%
Calka J.
Rozprawy i Monografie. Uniwersytet Warmińsko-Mazurski w Olsztynie
|
2002
|
nr 64
1-46
2

Increased expression of CART, nNOS, VIP, PACAP, SP and GAL in enteric neurons of the porcine stomach prepyloric region following hydrochloric acid infusion

100%
Calka J.
Folia Histochemica et Cytobiologica
|
2019
|
tom 57
|
nr 4
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Wegetarianizm i weganizm mogą negatywnie wpływać na inteligencję i zdrowie psychiczne

100%
Calka J.
Życie Weterynaryjne
|
2023
|
tom 98
|
nr 04
4

The role of nitric oxide in the hypothalamic control of LHRH and oxytocin release, sexual behavior and aging of the LHRH and oxytocin neurons

100%
Calka J.
Folia Histochemica et Cytobiologica
|
2006
|
tom 44
|
nr 1
5

Unijne standardy studiow weterynaryjnych

88%
Calka J.
Życie Weterynaryjne
|
2005
|
tom 80
|
nr 06
323-324
6

Zielone panaceum

75%
Calka J.
Wiedza i Życie
|
2007
|
nr 09
34-35
7

Analysis of expression of SP and NOS in the porcine nodose ganglion (NG) sensory neurons supplying prepyloric stomach region after intragastric hydrochloric acid infusion

63%
Rytel L., Calka J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: We anylazed expression of substance P (SP) and nitric oxide synthase (nNOS) in the porcine nodose ganglion sensory neurons innervating prepyloric stomach region in physiological state and following intragastric hydrochloric acid infusion. METHODS: The study was performed on 8 immature gilts of the Large White Polish breed. All animals were injected retrograde neuronal marker Fast Blue (FB) into the anterior prepyloric stomach wall and then divided into 2 groups (n=4 each). On 23rd day after FB injection gilts of the HCL group received single infusion of hydrochloric acid into the stomach. On 28th day all control and HCL pigs were euthanized and bilateral right (rNG) and left (lNG) were collected. Cryostat sections were processed for double immunofluorescence using antibodies against SP and NOS. RESULTS: Immunofluorescence staining of the nodose ganglia in control group showed the presence of FB-positive (gastric) neurons expressing SP (45.9±3.38% in rNG and 60.4±1.71% in lNG) and NOS (34.9±6.83% in rNG and 49.9±9.32% in lNG). The SPpositive neurons revealed granular immunoreaction product evenly distributed throughout the cytoplasm. The NOS immunostaining appeared as smooth immunoprecipitate observed throughout the cytoplasm. In HCL group increased expression of SP in the rNG (54.8±5.34%) and decreased in lNG (56.9±3.28%) was found in gastric neurons. While number of FB+/NOS-immunoreactive perikarya increased in both rNG (54.9±4.45%) and lNG (52.5±2.17%) respectively. Both appearance as well as distribution of immunoreaction products resembled that in control group. CONCLUSIONS: The acquired results suggest that SP and NOS function as neurotransmitters/neuromodulators in the vagal sensory transduction from prepyloric region of the porcine stomach. Additionally, they are possibly involved in pathological changes related to hyperacidity induced gastritis.
8

An ultrastructural demonstration of NADPH-diaphorase-nitric oxide synthase activity in the rat striatal astroglia

63%
Calka J., Wolf G.
Folia Morphologica
|
2003
|
tom 62
|
nr 3
251-253
In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplasmic reticulum and mitochondria. Predominantly mitochondria of astrocytic “end feet” were labelled. Our ultrastructural observations promote the possibility that astroglial NADPH-d/NOS is involved in adaptation of the local blood flow in the striatal microenvironment.
9

Changes in expression of somatostatin in the CSMG neurons supplying prepyloric area of the porcine stomach induced by intragastric infusion of hydrochloric acid

63%
Palus K., Calka J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Gastric hyperacidity is frequent gastric disorders. However, little is known about the changes in the expression of somatostatin (SOM) in the the coeliac-superior mesenteric ganglion (CSMG) neurons supplying prepyloric area of the porcine stomach during inflammation induced by intragastric infusion of hydrochloric acid. The present study was designed to define localization and chemical expression of somatostatin in the sympathetic perikarya supplying the porcine stomach prepyloric area in physiological state and during gastritis induced by intragastric infusion of hydrochloric acid. METHODS: Ten juvenile female pigs of the Large White Polish breed were used. The animals were divided into two groups: control and animals with hydrochloric acid infusion (HCl). The neuronal retrograde marker Fast Blue (FB) was injected into the anterior prepyloric wall of the stomach of all animals. 23 days after FB injection, the animals of HCI group were introduced into a state of general anesthesia and given intragastrically 5 ml/kg of 0.25 M hydrochloric acid solution. 28 days after FB injections all animals were deeply anaesthetized, transcardially perfused with buffered paraformaldehyde and tissue samples were collected. The CSMG cryostat sections were stained immunocytochemically for SOM and TH (tyrosine hydroxylase). RESULTS: In the control group 14.97±1.57% out of 200 FBpositive CSMG neurons contained SOM. Inflammation induced by intragastric infusion of hydrochloric acid resulted in upregulation of the SOM-IR neurons to 29.63±0.85%. All SOM-IR neurons in both groups showed the simultaneously TH immunoreactivity. CONCLUSIONS: Increase in the expression of SOM in FB-possitive neurons of the HCL group may suggest its participation in the protective mechanisms of neurons in different pathological processes, such as gastric hyperacidity
10

Diabetes mellitus in cats relevant to human type 2 diabetes – current knowledge and new treatment strategies – a review

63%
Palus K., Calka J.
Annals of Animal Science
|
2015
|
tom 15
|
nr 1
11

Study of chemical coding of the CSMG neurons supplying prepyloric region of the porcine stomach following acetylsalicylic acid supplementation and after partial stomach resection

63%
Calka J., Palus K.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: The present study was designed to define localization and chemical coding of the sympathetic perikarya innervating the porcine stomach prepyloric area in physiological state, following prolonged aspirin supplementation and after partial stomach resection. METHODS: The study was performed on 3 groups of 5 immature female pigs. The neuronal retrograde marker Fast Blue (FB) was injected into the anterior prepyloric wall of the stomach of control, acetylsalicylic acid treated (ASA) and partial stomach resection (RES) groups. Animals in ASA group were given acetylsalicylic acid orally for 21 days. On 22nd day after injection, in animals of RES group partial stomach resection was performed. On 28th day all pigs were euthanized. Then cryostat sections were double immunolabeled using primary antisera directed towards tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), galanin (GAL), somatostatin (SOM), nitric oxide synthase (NOS), Leu 5-Enkephalin (LENK). As the secondary antibody AlexaFluor 546 and AlexaFluor 488 were applied. In the coeliacsuperior mesenteric ganglion (CSMG) 1615±20.73 FB+ (gastric) neurons have been identified. RESULTS: In the control group, gastric neurons expressed TH (94.85±1.01%), DβH (97.10±0.97%), NPY (46.88±2.53%), SOM (14.97±1.57%) and GAL (8.40±0.53%). In ASA and RES groups, TH- and DβH- positive nerve cells were reduced (ASA: 81.78±0.87% and 86.42±0.94%; RES: 84.56±2.56% and 88.30±1.62%). Moreover, in both groups increased expression of NPY (ASA: 76.59±3.02%, RES: 64.93±2.32%), SOM (ASA: 33.72±4.39%, RES: 39.02±3.65%), GAL (ASA: 26.45±2.75%, RES: 31.13±1.64%) as well as de novo synthesis of NOS (ASA: 6.13±1.11%, RES: 7.29±0.65%) and LENK (ASA: 4.77±0.42%, RES: 3.92±0.45%) in traced CSMG neurons were observed. CONCLUSIONS: Our results indicate involvement of studied substances in the development and presumably counteraction of gastric inflammation and survival of damaged neurons.
12

Expression of SP, CGRP, NOS, VIP and GAL in the porcine NG neurons supplying prepyloric stomach region following acetylsalicylic acid supplementation and partial stomach resection

63%
Calka J., Rytel L.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: We analyzed expression of SP, CGRP, NOS, VIP and GAL in the porcine nodose ganglion (NG) sensory neurons innervating prepyloric stomach region in physiological state, following gastritis evoked by acetylsalicylic acid supplementation and after partial stomach resection. METHODS: The study was performed on 12 immature gilts divided into 3 groups (n=4 each). All animals were injected retrograde marker Fast Blue (FB) into the anterior prepyloric stomach wall. Since 7th day after FB injection animals of the ASA group were given acetylsalicylic acid (100 mg/kg BW) orally for 21 days, while animals of RES group underwent partial stomach resection on 22nd day following FB injection. On 28th day all pigs were euthanized and bilateral right (rNG) and left (lNG) were collected. Cryostat sections were double immunolabeled using antibodies against SP, CGRP, NOS, VIP and GAL. RESULTS: Microscopic analysis of the control group revealed expression of SP (45.9±3.4% in rNG and 60.4±1.7% in lNG), CGRP (32.5±4.3% in rNG and 42.6±9.5% in lNG), nNOS (34.9±6.8% in rNG and 49.9±9.3% in lNG), VIP (17.9±2.7% in rNG and 31.5±5.1% in lNG) and GAL (21.9±3.3% in rNG and 35.5±6.8% in lNG) in FB+ perykaria. In RES group increased expression of SP (61.3±3.3% in rNG and 55.2±5.6% in lNG), CGRP (60.6±2.7% in rNG and 52.8±3.7% in lNG), nNOS (49.3±4.5% in rNG and 52.8±9.0% in lNG), VIP (61.3±2.5% in rNG and 67.1±6.8% in lNG) and GAL (35.1±7.3% in rNG and 37.1±5.8% in lNG) in FB+ perykaria was found. In ASA group increased expression of SP (56.5±3.4% in rNG and 67.2±3.9% in lNG), CGRP (52.8±6.9% in rNG and 64.8±6.2% in lNG), nNOS (47.9±9.0% in rNG and 59.3±7.1% in lNG), VIP (51.9±6.0% in rNG and 60.3±5.6% in lNG), GAL (47.2±7.2% in rNG and 55.8±4.2% in lNG) in FB+ perykaria was observed. CONCLUSIONS: Our data suggest that SP, CGRP, NOS, VIP and GAL play a role in sensory transduction, posttraumatic dendrites regeneration, and development of the stomach.
13

Immunohistochemical characterization of neurons in the porcine ciliary ganglion

51%
Kaleczyc J., Juranek J., Calka J., Lakomy M.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
|
nr 1
14

Rodzinne schorzenia psów rasy shar-pei związane z podwyższonym poziomem IL-6

51%
Palus K., Rytel L., Calka J.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 08
The aim of this article is to present the current state of knowledge concerning the familial diseases of Chinese Shar-pei dogs, with particular emphasis on the pathogenesis and treatment options. Familial Shar-pei fever (FSF) is an autoimmune disease clinically resembling human hereditary periodic fever syndromes called familial Mediterranean fever (FMF). Hereditary predisposition to the disease occurs in 23% of the population of this breed, and it is due to the presence of mutations in a gene located within the gene encoding the synthetase-2 hyaluronic acid (HAS2) on the 13th chromosome. FSF is characterized by recurrent episodes of fever of unknown origin associated with elevated levels of IL-6 in serum. The most common complications of chronic inflammation include swelling of the joints, especially ankles, and organ amyloidosis, which interferes with the proper functioning of organs and leads to their chronic failure - and, consequently, to premature death or euthanasia.
15

Rozpoznawanie i leczenie zaburzen cyklu plciowego oraz ciazy u szczurow laboratoryjnych

51%
Juranek J., Buzon B., Jedlinska-Krakowska M., Calka J.
Magazyn Weterynaryjny
|
2006
|
tom 15
|
nr 05
63-65
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Immunohistochemical characterization of neurons and neuronal processes in the dorsal vagal nucleus of the pig

51%
Ganko M., Rychlik A., Calka J.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 1
The vagus nerve is responsible for efferent and afferent innervation of the gastrointestinal tract. The efferent fibres originate from neurons located in the dorsal motor nucleus of the vagus (DMX) and control mainly the gastric motor and secretory function. The main goal of our study was to examine expression of ChAT, SP, LENK, NOS and CART in the neuronal matrix of the porcine DMX. Double-labeling immunofluorescence revealed plenty of ChAT-IR neuronal cell bodies and fibres distributed throughout the nuclear matrix. Between the cholinergic somata and processes numerous SP-, LENK- and CART-IR neuronal protrusions forming dense networks were identified. While net of the NOS-IR fibres presented moderate density, the SP- and LENK-IR processes were often observed to form a basket-like structure closely surrounding the cholinergic parasympathetic neurons. Individual CART-IR basket-like formations were also encountered. A few double labeled ChAT-/NOS-IR perikarya in the rostral segment of the nucleus were also found. We confirm expression of studied antigens in the porcine DMX and provide morphological foundations for a possible regulatory role of SP, LENK, NOS and CART in porcine vago-visceral signaling.
17

Białka synaptycznej strefy aktywnej - koordynatorzy procesu neurotransmisji w układzie nerwowym

51%
Juranek J., Calka J., Lakomy M.
Humanistyka i Przyrodoznawstwo. Uniwersytet Warmińsko-Mazurski w Olsztynie
|
2007
|
tom 13
18

The localization and expression of NADPH-diaphorase and isoforms of nitric oxide synthase in the porcine gravid uterus

51%
Andronowska A., Chrusciel M., Calka J.
Reproductive Biology
|
2008
|
tom 08
|
nr 3
19

Co-localisation of nos- and chat-immunoreactivity in the spinal autonomic nuclei of the pig. An immunocytochemical study

51%
Calka J., Zalecki M., Arciszewski M. B.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 4
Our previous studies revealed an overlap of cholinergic and NO-ergic preganglionic neurons in the autonomic sympathetic and parasympathetic spinal cord nuclei of the pig. A close topographical as well as morphological relationship between both neuronal populations suggests a possible co-expression of the ChAT and NOS in the same autonomic neurons. Therefore, the goal of the present study was to determine the correlation between the ChAT- and neuronal NOS-immunoreactivity in the thoracic intermediolateral and sacral intermediolateral and intermediomedial nuclei. Double immunocytochemical staining revealed that many of the ChAT positive cell bodies demonstrated immunoreactivity to NOS. The number of the double stained cell bodies depended on the cross-section level. In the intermediolateral nucleus of the thoracic spinal cord about 60% of the ChAT-positive cells co- expressed also NOS, while in the intermediolateral nucleus of the S2 spinal segment constituted approximately 52%. The colocalisation of both substances in the intermediomedial nucleus varied from 39% at SI level until 58% at S2 spinal plane. Our data provide morphological evidence confirming the co-expression of NOS in the spinal sympathetic and parasympathetic cholinergic neurons and suggest that NO may play a role in the modulation of the spinal cholinergic transmission.
20

Niektore aspekty leczniczego dzialania zielonej herbaty

51%
Calka J., Zasadowski A., Juranek J.
Bromatologia i Chemia Toksykologiczna
|
2008
|
tom 41
|
nr 1
5-14
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