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  1. 29 Herba Polonica

  2. 2 Herba Polonica. Suplement

  3. 2 Polish Journal of Veterinary Sciences

  4. 2 Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Biologia i Hodowla Zwierząt

  5. 2 Żywienie Człowieka i Metabolizm. Suplement

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  1. 41 Mielcarek S.

  2. 27 Buchwald W.

  3. 25 Mrozikiewicz P. M.

  4. 20 Krajewska-Patan A.

  5. 20 Mscisz A.

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  1. 3 2013

  2. 5 2012

  3. 4 2011

  4. 1 2010

  5. 8 2009

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1

The content of flavonoids and polyphenolic acids in inflorescences of sandy everlasting [Helichrysum arenarium [L.] Moench] from natural stands and plantations

100%
Sawilska A. K., Mielcarek S.
Herba Polonica
|
2009
|
tom 55
|
nr 3
118-126
During the years 2007–2008 the content of flavonoids and polyphenolic acids in inflorescences of Helichrysum arenarium (L.) Moench was analyzed in specimens from natural stands and plantations. Plants cultivated in experimental plots originated from in vitro plantlets. The amount of flavonoids in inflorescences from experimental plots was higher and attained its maximum at 1.19% compared with 0.84% observed for plants from natural stands. In herbal material collected in 2008 the content of flavonoids was lower. The tendency was not observed for polyphenolic acids. Within the study period their amount ranged over the interval 0.74–2.82%. It was also found that dry mass of inflorescences from natural stands was higher than that for cultivated plants by 30.9%. In contrast, the density of generative shoots was 5.2-fold higher in natural stands. It was shown that Sandy Everlasting plantations could yield up to 3.4-fold more herbal material which is of a better quality (contains more biologically active compounds) than that from natural stands.
2

Badania fitochemiczne jako czynnik ulatwiajacy ocene bezpieczenstwa i jakosci produktow leczniczych i suplementow diety zawierajacych przetwory z Echinacea i Ginkgo biloba

100%
Mielcarek S., Mscisz A.
Herba Polonica
|
2007
|
tom 53
|
nr 2
69
3

Phytochemical investigations as a tool for safety and quality evaluation of medicinal products and dietary supplements containing Echinacea and Ginkgo biloba preparations

100%
Mielcarek S., Mscisz A.
Herba Polonica
|
2007
|
tom 53
|
nr 2
4

The effect of thermal and freeze drying on the content of organic acids and flavonoids in fruit of European cranberry [Oxycoccus palustris Pers.]

81%
Adamczak A., Buchwald W., Kozlowski J., Mielcarek S.
Herba Polonica
|
2009
|
tom 55
|
nr 3
94-102
The present paper shows the results of a study on the effect of freeze and thermal drying on the content of organic acids and flavonoids in fruit of European cranberry (Oxycoccus palustris Pers.). They indicate a significant effect of drying conditions on the content of the above mentioned active compounds. In lyophilized fruit, distinctly more organic acids but less flavonoids were found than in fruit dried at a temperature of 35–400 C. The largest relative differences were observed in the case of ascorbic acid. Its content in the fruit dried at high temperature, compared to the lyophilized material, was lower by a mean value of 5 mg/100 g of dry matter (DM), that is, by 42% on the average. With regard to the other organic acids (quinic, citric and malic acids), the average difference was 0.9–2.1 g/100 g DM (14–17%). The mean flavonoid content in the thermally dried fruit was larger by 34 mg/100 g DM (converted into hyperoside) and 24 mg/100 g (converted into quercetin), that is on average by 19% as compared to freeze-dried samples.
5

Standaryzacja zielarskich przetworów spożywczych

81%
Segiet-Kujawa E., Iberhan L., Mielcarek S.
Żywienie Człowieka i Metabolizm. Suplement
|
2004
|
tom 31
|
nr 1 cz.1
6

Produkty immunomodulujace z Echinacea. Polifenolokwasy i alkamidy - standaryzacja technika RP-HPLC-DAD

81%
Mielcarek S., Mscisz A., Otta H., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
41-42
7

Metoda oznaczania zawartosci melatoniny technika ultrasprawnej chromatografii cieczowej [UPLC]

81%
Przybylak J. K., Mielcarek S., Mscisz A., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
45-46
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effect of air freeze drying on the content of flavonoids, beta-carotene and organic acids in European dog rose hips [Rosa L. sect. Caninae DC. em. Christ.]

81%
Adamczak A., Buchwald W., Zielinski J., Mielcarek S.
Herba Polonica
|
2010
|
tom 56
|
nr 1
7-18
This paper presents the results of a study on the effect of air drying (at a room temperature) and freeze drying on the content of flavonoids, β-carotene and organic acids in total weight of hips (hypanthia and achenes) of roses from the section of Caninae DC. em. Christ. The obtained results show a significant effect of drying conditions on the content of β-carotene and organic acids. In the lyophilized material more organic acids were found, but less β-carotene, than in the rose hips dried at a room temperature. The largest differences were noted in the case of ascorbic acid. Its content in the lyophilizates was on average 5 times higher than in the air-dried hips and it was on average as much as 1.225 g/100 g of dry weight (DW). The amount of citric acid was higher by only 10%, on average. Large differences were also found for β-carotene. Its content in the freeze-dried material, compared to the air-dried rose hips, was on average lower by 74 mg/100 g DW (43%). In the case of flavonoids, the obtained results were ambiguous.
9

Standaryzacja zielarskich przetworow spozywczych

81%
Segiet-Kujawa E., Iberhan L., Mielcarek S., Mrozikiewicz P. M.
Żywienie Człowieka i Metabolizm. Suplement
|
2004
|
tom 31
|
nr 2 cz.1
452-461
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The determination of flavan-3-ol content in the root of Rhodiola kirilowii

81%
Gryszczynska A., Mielcarek S., Buchwald W.
Herba Polonica
|
2011
|
tom 57
|
nr 1
According to a new classification, nearly 90 species belong to the Rhodiola family. However, only three of them are most frequently used in alternative medicine. In addition to R. rosea and R. quadrifida, R. Kirilowii can also be included in this group. This species was investigated for flavan-3-ol content. Due to beneficial properties of this class of compounds (free radical scavenging and reactive oxygen forms), the researchers decided to examine 5 substances in the root and in two extracts, alcoholic and aqueous ones. Depending on the matrix analysed, the content of particular components varies. Furthermore, it is also affected by extraction time as well as a particular extraction solvent employed. An ultra performance liquid chromatograph connected to a tandem mass spectrometer (Waters) was used for the assay. The application of this analytical method allows to detect very small amounts of analytes.
11

Zawartość olejku eterycznego i kwasu walerenowego w korzeniach kozłka lekarskiego (Valeriana officinalis L.) w wybranych fazach fenologicznych

81%
Seidler-Lozykowska K., Baraniak M., Mielcarek S.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 527
W latach 2005 i 2006, w Instytucie Roślin i Przetworów Zielarskich w Poznaniu przeprowadzono analizę zawartości wybranych substancji czynnych w surowca kozłka lekarskiego w dziewięciu następujących fazach fenologicznych w drugim roku wegetacji: początek wegetacji, rozeta wiosenna, wykształcanie pędów kwiatostanowych, początek kwitnienia, pełnia kwitnienia, zbiór nasion, trzy tygodnie po zbiorze nasion, rozeta jesienna, po pierwszych przymrozkach. Celem badań było zbadanie zależności pomiędzy zawartością olejku i kwasów walerenowych, a fazami fenologicznymi co pozwoliłoby ustalić optymalny termin zbioru surowca. Materiał do badań stanowiły korzenie kozłka odmiany Polka oraz rodu hodowlanego nr 4 pobierane losowo w każdej fazie. W surowcu oznaczono zawartość: olejku eterycznego, kwasu walerenowego, kwasu acetoksywalerenowego, kwasu hydroksywalerenowego oraz sumę kwasów walerenowych. Największe ilości olejku eterycznego oraz kwasów walerenowych stwierdzono w różnych fazach wegetacji: u rodu 4 w fazie pełni kwitnienia (1,3% i 0,266% odpowiednio), natomiast u odmiany Polka - w trzy tygodnie po zbiorze nasion (zaw. olejku 1,25%), a kwas walerenowy - w fazie zbioru nasion (0,218%). Najmniejsze zawartości olejku w surowcu występowały na początku wegetacji oraz po pierwszych przymrozkach jesiennych. Wyniki badań sugeruj ą, że wysokie zawartości olejku oraz sumy kwasów walerenowych w drugim roku wegetacji można uzyskać w surowcu kozłka od pełni kwitnienia roślin (0,278%) do fazy rozety jesiennej (0,316%).
12

Differentiation of Pimpinella saxifraga L. s.l. in comparative, morphological and phytochemical analyses

71%
Latowski K., Komorowska E., Grys A., Mielcarek S., Buchwald W.
Herba Polonica
|
2009
|
tom 55
|
nr 3
11-19
The paper presents study results on Pimpinella saxifraga L. s.l. which belongs to critical species whose taxonomy has not been finally determined, so far. Because of the fact that in this genus, there occur species with acknowledged usefulness (P. anisum L., and P. anisetum Boiss. et Ball) and possess therapeutic importance [among others: P. saxifraga L., P. tragium Vill., P. major (L.)Huds.], the presented studies can be helpful in the determination of their natural affinity. Burnet saxifrage, P. saxifraga, was the research object treated as an aggregation of forms which are related to each others because of a high degree of structural polymorphism. Morphological studies were carried out on herbarium material collected in 34 localities in West Poland (Wielkopolska-Kuyavia Lowland). Results of this part of studies that in that have shown terrain, there occur two separate forms (“nigra” and “saxifraga”), whereby the “nigra” form occurs significantly more frequently than the “saxifraga” one. The ranges of biometrical measurements of some features are overlapping indicating that there occur plants with an intermediate character (hybrids?). In the phytochemical analysis of roots, it was that there occurred essential oil which contained eight components. The domination of the “nigra” form has been confirmed. Its essential oil different than the oil of the “saxifraga” form, it has a different colourand its total content is usually increased. In the composition of both forms (“nigra” and “saxifraga”), there definitely dominate myrcene and limonene. The “nigra” form is characterized by the absence of cymene, while “saxifraga” form has no γ-terpinene and only some traces of cymene and sabinene
13

Safety of John's wort administered during lactation in pigs

61%
Mrozikiewicz P. M., Mscisz A., Majewski P., Koziorowski M., Mielcarek S., Iwanik K., Gorski M.
Herba Polonica. Suplement
|
2005
|
tom 51
|
nr 1
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

61%
Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.
Herba Polonica
|
2012
|
tom 58
|
nr 3
The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.
15

Centella asiatica uprawiana w Polsce - badania fitochemiczne metoda RP-HPLC-DAD

61%
Mielcarek S., Przybylak J. K., Buchwald W., Furmanowa M., Krajewska-Patan A., Mscisz A., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
79-80
16

Uprawy probne Centella asiatica [L.] Urban w Polsce

61%
Buchwald W., Forycka A., Furmanowa M., Mscisz A., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
67
17

Wpływ podawania kurczętom brojlerom fitopreparatu przed obrotem przedubojowym na właściwości fizykochemiczne mięsa

61%
Wojcik A., Sowinska J., Mituniewicz T., Witkowska D., Mielcarek S., Murawska D., Pomianowski J. F.
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Biologia i Hodowla Zwierząt
|
2011
|
tom 63
18

Evaluation of the analytical method for a chromatographic profile assessment of the essential oil obtained from Pimpinella saxifraga s.l.

61%
Grys A., Komorowska E., Mielcarek S., Lowicki Z., Latowski K., Buchwald W.
Herba Polonica
|
2009
|
tom 55
|
nr 3
109-117
Pimpinella saxifraga s.l. has been used for medicinal purposes for a long time. The root is a part of medicinal properties composed by essential oils in about 0.4%. The composition of these essential root oils consists of about sixty chemicals. The aim of the current experiment was assessing the analytical method of chromatographic profile utilizing essential oil of Pimpinella saxifraga. This essential oil was isolated with use of Deryng apparatus according to Polish Pharmacopoeia. The method for assessing the chromatographic profile of the essential oil was split-up gas chromatographic capillary column. The quantitative composition of the essential oil was calculated with use of method of normalization. The identity and the compositions of the essential oil from Pimpinella saxifraga roots was analyzed on Clarus 500 gas chromatograph equipped with capillary columns with flame ionization detector (FID), automatic dispenser and computer with Total Chrom Navigator software Perkin Elmer. Chromatographic system was characterized by range of retention time, area peaks, tailing factor and compounds’ separation. This method of volatile oil substance marking was validated following the ICH standards, by its precision, accuracy and linearity.
19

Wplyw nawozenia mineralnego na zawartosc zwiazkow biologicznie czynnych i mase korzeni Salvia miltiorrhiza Bunge

61%
Buchwald W., Forycka A., Mscisz A., Mielcarek S., Mrozikiewicz P. M., Baraniak M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
65
20

Zmiany zawartosci zwiazkow biologicznie czynnych w korzeniach Salvia miltiorrhiza Bunge w okresie wegetacji

61%
Buchwald W., Forycka A., Mscisz A., Mielcarek S., Mrozikiewicz P. M., Baraniak M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
64
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