Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 10

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
A computer – aided semen analyzer (CASA) was used for monitoring some motility parameters of zebra mussel sperm after spawning for up to 1,5h at 22°C and after 24 hours of storage at 8°C. The mean percentage of motility (MOT) for sperm shortly after spawning was 70ą2.0 %, VSL = 31.8ą3.0 µm s-1, and VCL= 110.2ą6.4 µm s-1. The motility expressed as LIN was 27.7ą2.0 %, STR=44.5ą4.8 %, ALH=8.7ą2.0 µm, BCF=6.8ą2.0 Hz. The data demonstrate a consistent decrease in VSL (12.8ą 4.0), VCL (57.4ą 5.7), LIN (15.8ą 3.0) and ALH (1.1ą 0.23) values after 24 hours. No significant differences were found for % of MOT, BCF and STR parameters values during the time of experiment. We found CASA analyzer as a proper tool helpful to discerning the quality of zebra mussel semen samples.
The objectives of this study were: sperm cryopreservation, computer assisted sperm motility analysis (CASA) and improvement of sperm motility through in vitro incubation of rainbow trout neomale spermatozoa. In vitro incubation of sperm in ASP (Artificial Seminal Plasma) solution resulted in an increase of the percentage of motile spermatozoa, while other sperm motility characteristics remained unchanged. The highest hatching rates were obtained for sperm cryopreserved in straws (16.3% and 25.0%): cryopreservation in pellets was less successful (7.7% and 3.8%), for non-incubated and incubated sperm, respectively. There were significant regressions between hatching rates and straight-line velocity (VSL; R² = 0.75) and average path velocity (VAP; R² = 0.74) for sperm preincubated in ASP and cryopreserved in straws. These data indicate that sperm of rainbow trout neomales may be successfully cryopreserved and CASA analysis is useful for the prediction of fertilizing ability of cryopreserved sperm.
Five-month-old male rates were exposed to 0.5 ppm ozone for 50 days, 5 hours a day. A week before the completion of ozone exposure, a biological test was performed to determine the fertilization rate and the survival rate of newborns in both ozone-exposed and control animals. After 50 days, the rats were sacrificed with an overdose of halotane, and testes were collected to assess the morphology and motility of spermatozoa. Neither the morphology of spermatozoa nor motility parameters determined by the CASA (computer-assisted sperm analysis) system showed statistically significant differences between ozone-exposed and control males. The number of successful matings and the survival rate of newborns per litter within one year postpartum were also similar in both groups. However, sperm concentration was by 17% lower in ozone-exposed rats, compared with the control animals.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.