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Effect of plant phenolics isolated from leaves of blackcurrant (Ribes nigrum L.) and sour cherry (Prunus cerasus L.) as potential biopesticides against the grain aphid was examined. The extracts from the sour cherry contained a twice higher level of salicylic acid than extract from leaves of the blackcurrant. Moreover, concentration of chlorogenic acid was higher in sour cherry and the content of tannic acid within both plant tissues was at the same level. Both examined extracts had a negative effect on development of the grain aphid when the aphid fed on wheat plants sprayed with 1.0%, 2.5% and 5.0% solutions. The length of prereproductive period (time from birth to maturity) of the aphids was extended from 5.9 days on control plants (not sprayed) to 8.1 days, when the aphids were fed on the treated plants. Apterous females of Sitobion avenae fed on sprayed plants showed lower fecundity and lower values of intrinsic rate of natural increase.
Phenolic compounds were extracted with 60% methanol (after purification with acetone) from the ears of two winter triticale cultivars (Dagro and Malno). Total phenols were determined and five phenylpropanoid acids were identified. Separation of phenylpropanoids was carried out in a H PLC column - C,8 Eurospher with the use of KNAUER isocratic system. A mixture of methanol- -0.1 M KH2P04 (55: 45) was used as a mobile phase. The obtained results showed that the contents of total phenols and caffeic acid were higher in Malno than in Dagro. The concentration of four other phenylpropanoid acids was higher in Dagro.
Wykorzystując wysokosprawną chromatografię cieczową (HPLC) zbadano występowanie i oznaczono zawartość związków polifenolowych w liściach dwóch odmian pszenżyta ozimego (Dagro i Malno). Wyodrębniono frakcję wolnych polifenoli w siedmiu fazach rozwojowych pszenżyta i poddano ją analizie chromatograficznej na kolumnie z odwróconymi fazami (Eurospher C-18) w warunkach izokratycznych. Jako fazę rozwijającą zastosowano metanol - 0, IM KH2P04 (55:45). Zidentyfikowano i oznaczono zawartość pięciu związków polifenolowych, tj. katecholu, kumaryny, kwasu chlorogenowego, rutyny i (-)-epikatechiny. Dominującym polifenolem liści badanych odmian pszenżyta ozimego był kwas chlorogenowy. Przeprowadzone badania dowiodły, iż skład jakościowy i wysoka zawartość oznaczonych polifenoli w liściach odmiany Malno ma duży wpływ na jej konstytucyjną odporność na mszyce zbożowe.
The total amount of the identified phenolics was similar in leaves of both cultivars noninfested by the insects. However, higher content of ferulic acid and o-coumaric acid was found in the less acceptable triticale (Lamberto cv.) than in more acceptable Marko cv. After infestation an increase in content of the most phenolics was observed. The cereal leaf beetle brought the highest changes in the gallic, chlorogenic, salicylic, syringic and ferulic acid. The grain aphid feeding resulted in the increase of gallic, chlorogenic, vanilic, caffeic, syringic and salicylic acids. Moreover, much higher induction of phenolic biosynthesis occurred within seedlings of Lambero cv. than in Marko one. The differences in phenolic acids level within triticale tissues induced by the chewing and sucking-piercing insects are discussed.
The effect of methyl jasmonate (MJ) vapors on content of phenolic compounds: free phenolic acids, total quercetin, and total phenolics in etiolated buckwheat seedlings were studied. The data presented show that low concentration of MJ (10-8 M) had no influence on trans-cinnamic acid (CA), but stimulated the accumulation of chlorogenic acid in hypocotyls and cotyledons of buckwheat seedlings. A moderate dose of MJ (10-6 M) did not change the level of chlorogenic acid in the hypocotyls and cotyledons, but CA synthesis was promoted in cotyledons, whereas in hypocotyls no significant effect was found. Highest concentration of MJ (10-4 M) caused small decline of CA in hypocotyls, but large stimulation of the acid production in cotyledons was noted. MJ had stimulatory effect on caffeic acid forming, but inhibited synthesis of vanillic acid in hypocotyls and cotyledons. Lowest concentration of MJ (10-8 M) elicited accumulation of quercetin glycosides in both studied tissues of buckwheat seedlings, however at higher doses (10-6 and 10-4 M) did not affect the flavonol level. The obtained results suggest that nonequivalent influence of methyl jasmonate on the phenolics composition can be a result of various mechanisms of MJ uptake, transforming and/or its translocation in buckwheat hypocotyls and cotyledons. Decline of anthocyanins level in buckwheat hypocotyls caused by MJ cannot be explained by enhanced accumulation of quercetin glycosides or free phenolic acids, but probably by synthesis of other unknown phenolic compounds.
It was confirmed during laboratory tests that two cultivars of spring triticale (Kargo and Migo) developed the highest level of antibiosis against Sitobion avenae, whereas the lowest one was found for Gabo seedlings. Values of daily fecundity (DF) and intrinsic rate of natural increase (rm) were significantly higher for aphids developed on the susceptible cultivar Gabo. Field experiments have shown that cultivars with lower level of antibiosis (Gabo and Mieszko) evolved a stronger ability to recovery of plant injuries and reduction of yield loss caused by the grain aphid. On the other hand, we have found lack differences in density of S. avenae migrantes on the tested cultivars. Thus, these results demonstrate that plants of spring triticale develop two mechanisms of resistance against aphids: antibiosis and tolerance.
Host specialization plays a central role in physiological diversification of aphids and plant-insect co-evolution. Different morphs of the bird cherry-oat aphid (Rhopalosiphum padi L.) are specialized and locally adapted to both primary and secondary hosts. The abundance of bird cherry-oat aphid population was estimated using the technique of direct counting of aphid individuals infesting 50 randomly selected, fully expanded 1-year side shoots of bird cherry (approx. 40 cm long) grown in the Municipal Park „Aleksanria” in Siedlce (central-eastern Poland). Entomological observations were conducted during three consecutive vegetation seasons: 2001–2003. The impact of R. padi feeding on composition of free amino acids within leaves of its primary host (the bird cherry, Prunus padus L.), infested and uninfested by aphids were studied. Eight essential amino acids (Val, Thr, Phe, Ileu, Leu, Met, His and Lys) and seven non-essential amino acids (Asp, Glu, Pro, Ser, Ala, Gly and Tyr) were identified in the bird cherry-oat leaves with the use of highperformance liquid chromatography (HPLC) separation. There were quantitative differences in free amino acids content between the tested bird cherry-oat leaves (infested and uninfested by aphids). Levels of the free amino acids were decreased in leaves attacked by R. padi, and detailed analyses revealed about 20% decline in the content of studied components during maximal aphid abundance in comparison with non-infested leaves. Additionally, the feeding behavior of the bird cherry-oat aphid seasonal morphs (fundatrix, fundatrigeniae, alatae) on the primary host was investigated. Using the electrical penetration graph (EPG) we showed differences in feeding behavior of bird cherry-oat aphid morphs on primary host. The total probing time was greatest for fundatrigeniae among all studied morphs. These individuals spent more time during phloem sap ingestion then others. Adults of alatae were not active in the phloem sap ingestion on the bird cherry. Among the tested morphs xylem sap ingestion activity was greatest for alatae. It is hypothesized that rejection of primary host and subsequent the bird cherry-oat aphid migration onto secondary hosts may be stimulated by the decline in levels of free amino acids within primary host leaves caused by long-term R. padi feeding. Comparative analyses of the aphid probing behavior additionally proved that P. padus leaves possessed high nutritive value of food for non-winged morphs (early stages of aphid infestation), while not sufficient for winged alatae (terminal phase of infestation).
Kumestrol - 8 lakton kwasu 2,4-dihydroksyfenylo-6-hydroksy-3-benzofuranokarboksylowego (C15H18O5) to związek chemiczny o charakterze izoflawonoidu zaliczany do wtórnych metabolitów biorących czynny udział w reakcjach odpornościowych u roślin. Podjęte badania miały na celu wykrycie i oznaczenie zawartości kumestrolu w dwóch odmianach pszenżyta ozimego o różnym stopniu podatności na Sitobion avenae (F). (Dagro podatne, Lasko względnie odporne), w oparciu o podane niżej metody. Ekstrakcję i oczyszczanie kumestrolu ze świeżego materiału roślinnego, zebranego w 5 fazach rozwojowych, wykonano zmodyfikowaną metodą Liwingstone'a i wsp. 1961. Oczyszczone ekstrakty zagęszczano i po doprowadzeniu metanolem do 25 ml analizowano wykorzystując zmodyfikowaną, podwójną, jednokierunkową TLC i HPLC chromatografie (Loper 1968, Graham 1991). Zawartość kumestrolu w badanym materiale roślinnym odczytano z krzywej wzorcowej sporządzonej dla odpowiednich stężeń kumestrolu. Identyfikację kumestrolu przeprowadzono również w oparciu o widmo absorbcyjne roztworu standardowego w zakresie 200-350 nm, które analizowano na spektrofotometrze diodowym. Zastosowane techniki chromatograficzne (TLC i HPLC) oraz spektrofotometria absorpcyjna w UV dowiodły, że są niezwykle efektywne przy wykrywaniu, identyfikacji i oznaczaniu zawartości kumestrolu w badanych odmianach pszenżyta ozimego. Wykazały również, że wysoki poziom kumestrolu, stwierdzony w każdej z analizowanych faz rozwojowych w odmianie Lasko, determinuje prawdopodobnie jej wysoki stopień odporności w stosunku do S. avenae.
The influence of common plant polyamines on feeding behaviour and survival of grain aphid (Sitobion avenae F., Hemiptera: Aphididae) has been studied. Laboratory tests showed that wingless females of S. avenae assimilated higher quantities of food from triticale seedlings placed in 0.01% solutions of agmatine and cadaverine, and lower in case of spermidine and spermine. Increase in the polyamines concentrations up to 0.10% caused strong decrease in food assimilation, especially under the agmatine, cadaverine and putrescine treatment. Moreover, 0.10% concentration of the all analyzed polyamines decreased body mass and reduced survival of grain aphid wingless females. The 0.01% concentration of the polyamines were not affecting survival and body mass of S. avenae with exception of spermidine. Importance of the polyamines as potential natural biopesticides to the grain aphid is discussed.
The aim of the study was to determine the activity of ß-fructofuranosidase isolated from the bird cherry leaves collected in the period of spring development (April-May). The extracted enzyme was subjected to preliminary purification with ammonium sulphate and further purification with the use of column chromatography (Sephadex G-150). The obtained results proved that extracts purified by means of chromatography contained a more active enzyme and lower protein content in comparison with the crude ones. Furthermore, a statistically significant gradual decrease in invertase activity in the bird cherry leaves was noticed during the analysed period of vegetation.
The host-plants of bird cherry-oat aphid were compared as to the content and composition of phenolic compounds. The gallic, chlorogenic, caffeic, siringic, o- and p‑coumaric, tannic, ferulic, sinapinic, salicylic, p‑hydroxybenzoic acids and /+/‑catechin were detected in the all studied host-plants of the bird cherry-oat aphid. Coumarin and vanilic acid only occurred within orchard grass and winter triticale tissues. Predominant compounds within P. padus leaves were /+/‑catechin, chlorogenic acid, p-hydroxybenzoic acid and tannic acid. Vanilic and salicilic acids were the major compounds of the orchard grass, whereas leaves of winter triticale contained mostly salicilic, chlorogenic and vanilic acids as well as coumarin.
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