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The samples of stored seeds of pea (Pisum sativum L.) were collected from 30 farmer markets. The mycobiota analysis showed presence of 15 fungal species and one species of insect Callosobruchus chinensis. The fungi such as Aspergillus flavus, A. niger, A. ochraceous, A. terreus were found to be dominant based on percent frequency of each in blotter method in unsterilized and sterilized seeds 18.9–7.9, 15.0–3.9, 12.2–3.7, 10.1–1.7, respectively, and in agar plate technique 17.9–8.3, 15.1–9.5, 12.8–5, 7.9.7–6.7, respectively. These species showed reduction in terms of weight loss, germination and protein content in pathogenicity testing. Essential oil extracted by hydrodistillation from fruits of Cuminum cyminum L. was evaluated against the most common occurring funi such as A. flavus and A. niger as well as the insect species C. chinensis and the oil exhibited high toxicity. The oil killed the tested fungi and showed thermostable nature at its minimum inhibitory concentration (MIC) of 400 ppm. The oil safely preserved pea seeds up to 120 days at 0.50 (1,000 ppm) and 0.76 ml (1,500 ppm) in polyethylene and jute bags of 500 ml capacity containing 400 g seeds separately. There were no changes in organoleptic appereance of food seeds during storage. The oil has beneficial effect on number of visible nodule formation and shoot and root dry biomass of 15-day-old plants in comparison to control sets. The cumin oil was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
Background: Preterm birth is a common cause for neonatal morbidity and mortality worldwide. Aim of the study: To compare perinatal outcomes in preterm and term neonates. Material and methods: The present cohort study was conducted in the Obstetrics and Gynecology Department of MMIMSR, Ambala, a rural tertiary care center of Northern India over a one-year period (January-December 2018). 2,997 antenatal women were recruited at gestation (≥28 weeks) with singleton live pregnancies. All participants were divided into two groups: Group I: Antenatal women delivering at gestation (≥37 weeks) and Group II: Antenatal women delivering at gestation (≥28 to <37-weeks. Onset of labor, mode of delivery, perinatal outcome including birth weight, Apgar scores, Neonatal Intensive Care Unit (NICU) admission, need for intubation, complications, and mortality were compared between groups. Results: Of 2,997 deliveries, 2,528 (84.4%) were full-term, 469 (15.6%) preterm [48(10.2%) very preterm and 421(89.8%) late preterm] deliveries. The most common mode of delivery in both full-term and preterm participants was spontaneous vaginal delivery (62.8% vs 60.4%) followed by cesarean section (36.6% vs 39.2%). Preterm neonates (especially very preterm) had significantly lower birthweights (p<0.001, OR: 0.898; 95% CI: 0.108-7.48), 1-minute (p=0.018; OR: 7.812; 95% CI: 1.06-57.69) and 5-minutes Apgar scores (p=.000; OR: 3.410; 95% CI: 1.79-6.48) as compared to moderate- to- late preterm neonates. NICU admission rate, need for intubation, neonatal complications, stillbirth and early neonatal deaths were significantly higher in preterm neonates (p=.000). The most common complication in preterm neonates was Respiratory Distress Syndrome 172(55.1%) followed by sepsis 41(54.7%). Conclusions: Preterm delivery, especially very preterm, was significantly associated with adverse perinatal outcome as compared to full-term delivery.
A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 µM kinetin (Kn), 4.5 µM BAP, and 5.5 µM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 µM BAP and 8.5 µM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l⁻¹ activated charcoal. Elongated shoot treated with 15 µM IBA, 5.7 µM IAA, and 11 µM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification.
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