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Seasonal changeability of soil enzyme activity has not been entirely known. The aim of the paper was presenting the seasonal changeability in enzyme activity in fresh forest sites. From 10/06/2007 to 20/06/2008 samples were taken seven times at 6-weeks' intervals from two sample plots representing sites Lśw, LMśw, BMśw and one for Bśw site. Activity of dehydrogenase, protease and urease have been marked in the soil samples. As a result of conducted research the minimum value for enzymatic activity was found in October 2007 and April 2008. Maximum values for the activity of studied enzymes were noted in January and June 2007 and 2008, while no statistically significant differences were found between enzymatic activity marked in June 2007 and June 2008.
The aim of the study was to determine the activity of dehydrogenases and urease in forest soil before and after the blowdown. The research was a continuation project which was carried out in 2007-2010. The research was carried out in the Przedbórz Forest Inspectorate. Statistically significant differences in enzyme activity were observed in the soil taken before and after the blowdown. The lowest differences in enzyme activity were detected on the BM22, LM 35 and BM36 plots. Our results show that trees and shrubs have an impact on the soil properties. The enzymatic activity of the soil in the humus horizons may be used in estimating change in the soil.
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Enzyme activity in forest peat soils

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The aim of the study was to determine the activity of dehydrogenases and urease in forest peat soils of different fertility. There were selected 23 experimental plots localised in central and northern Poland. The research was conducted on forest fens, transition bogs and raised bogs. The biggest differences in soil physical and chemical properties were detected between fen and raised bog soils while raised bog soils and transition bog soils differed the least. Statistically significant differences between particular subtypes of peat soils were observed for soil pH-H2O, pH-KCl, C/N ratio as well as the content of organic carbon, nitrogen, calcium and potassium. The highest average dehydrogenase activity in the soil surface level was observed in fen soils, and the lowest – in raised bogs soils. The results obtained on urease activity were similar. Differences in urease activity in the studied soil types were shown. Dehydrogenase activity did not reveal statistically significant diversity. The activity of urease was negatively correlated with the content of carbon, C/N ratio, hydrolytic acidity and moisture. Also the increase in enzymatic activity accompanied by the increase in pH has been observed.
Taking into account the progressive degradation of soils it is important to assess their quality. Soil quality depends on a large number of physical, chemical, biological and biochemical properties. In the publications available, there are presented three approaches regarding the use of soil properties to estimate soil quality: (1) the use of individual properties, (2) the use of simple indexes and (3) the use of complex indexes derived from combinations of different properties. The purpose of this study was to investigate the possibility to use enzymes as indicators of forest soil quality. Experimental plots (43) were located in central Poland. The study was carried out in a number of diverse fresh forest sites. To assess the quality of forest soils dehydrogenase and urease activity and the degree of base saturation were used. One of the final conclusions point out that enzymatic activity indicates current site condition as well as the changes that occur in soil better than soil physical and chemical properties. In other words, in comparison to soil enzymatic activity, soil physico-chemical properties constitute a less sensitive indicator of soil changes.
The aim of this study was to assess the possibility of using biological and biochemical parameters in the evaluation of forest soil quality and changes caused by land use. The study attempted to determine a relationship between the enzymatic activity of soil, the number of earthworms and soil physico-chemical properties. The study was carried out in central Poland in adjoining Forest Districts (Przedbórz and Smardzewice). In soil samples taken from 12 research plots, basic physico-chemical properties, enzyme activity (dehydrogenase, urease) and density and biomass of earthworms were examined. Enzyme activity showed a large diversity within the forest site types studied. The correlations between the activity of the enzymes studied and C/N ratio indicated considerable importance of these enzymes in metabolism of essential elements of organic matter of forest soils. Urease and dehydrogenase activity and earthworm number showed susceptibility to soil pH, which confirmed relationships between enzyme activity and abundance of earthworms and soil pH in H2O and KCl.
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The aim of this study was to determine the site−creating value of forest soil with lithological discontinuities. The second aim was to describe an influence of lithological discontinuities on the soil properties and forest communities. Tested soils were grouped according to the ability to create forest sites. The presence of discontinuities has influence on the development of the forest communities, floristic abundance and forest stand quality which is expressed by site index.
The aim of this study was to assess the effects of different types of land use (forest, tillage and pasture) on soil properties, especially enzymes activity. Our investigation was carried out on 53 research plots with 11 plots in broadleaved forest stands, 12 plots in mixed broadleaved stands, 10 plots in mixed coniferous stands, 9 plots on tillage and 11 plots on pasture. The soil samples were collected from a depth of 0–15 cm after removing the organic horizon. Contents of organic carbon and nitrogen, pH and soil texture were investigated. Furthermore, dehydrogenase and urease activity were determined. Significant differences in the enzyme activity between forest and agricultural soils were observed, thus demonstrating that enzyme activity is influenced by the organic matter content of the soil. The highest enzyme activity was recorded in the forest soil within broadleaved stands, whilst the lowest activity was found in tillage soil, because tillage soil contained significantly less organic matter. High enzymatic activity of pasture soils is the combined result of vegetation type and the lack of plowing.
Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.
Wpływ biomasy pochodzącej z resztek pozrę− bowych na właściwości gleb. Sylwan 164 (2): 110−117. DOI: https://doi.org/10.26202/sylwan.2019052. The aim of the study was to determine the effect of different management treatments of Norway spruce post−harvest logging residues on soil properties. The study compared the impact of residues chipping and arrangement into the stacks. The investigation was carried out in the Nowy Targ Forest District (southern Poland). 15 research plots were selected for the analysis (5 plots with wood chips, 5 with stacks and 5 control ones). In May 2018, on each research plot soil samples for laboratory analysis were collected from the surface horizon (0−10 cm depth). Basic properties (pH, carbon and nitrogen content, base cations content) and enzyme activity (dehydrogenase, urease and β−glucosidase) were determined. The obtained results confirm the influence of post−harvest logging residues management on soil properties. The significant differences in soil carbon content between soils of different plots variants were noted. Soils of research plots with wood chips and stacks were characterized by higher content of carbon compared to the control research plots. At the same time soils with post−harvest logging residues had a higher biochemical activity expressed by enzymatic activity. The nutrients released from decaying post−harvest loggingresidues stimulated the microbiological activity of the soils.
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