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The purpose of examinations was defined role of environment of school in interindyvidual transmisions. The yeast and yeast - like fungi isolated from the oral cavity, troat and nose from 270 healthy children, at the age of 6 to 15 years, from country and municipal schools. The frequency of occurrence of fungi in country was higher than in minicipal schools by the resambling biodifferentiation. Totally 13 species belonging to 5 genera were detected in the examined material: Candida, Rhodotorula, Saccharomyces, Saccharomycopsis i Trichosporon. Increasing part of S. capsularis was noted in ontocenoses of healthy children. The qualitative and quantitative changes testify about changes of environment. Simultaneously, they inform about timely condition of mycoflora of the examined ontocenosis and surroundings.
Biodiversity of mycocoenoses of oral cavity, throat and nose of children aged 6-15 years. The purpose of this study was analysis of biodiversity of mycoflora in selected ontocenoses of healthy children. The material for the study was consisted of swabs taken from oral cavity, pharynx, and nose of healthy children: 128 girls and 142 boys. The material for the study was collected in May and November, dividing the children into two age groups: 6-9 years and 10-15 years. A total of 13 species representing 5 genera: Candida, Rhodotorula, Saccharomyces, Saccharomycopsis and Trichosporon were found in the material studied. The dominant group were yeasts-like fungi. Candida albicans, the basic etiological factor of the majority of mycotic diseases in humans, was found most frequently. Appearance of Candida glabrata and C. krusei is a reason for concern, as increasing numbers of their strains are resistant to basic antimycotic drugs, as weil as relatively frequent appearance of Trichosporon beigelii, Saccharomycopsis capsularis, and Saccharomyces sp. - the fungi showing increasing expansiveness and enzymatic activity. The fungi were most frequently isolated from oral cavity and pharynx. No phenological changes in fungal prevalence were found in the pharynx. In the other ontocenoses fungi were found much more frequently in the spring than in the autumn. Regardless of the season, the largest fluctuations in biodiversity and numbers of the studied mycocoenoses were observed in the oral cavity and nose - the organs that come into direct contact with environmental contaminants and many agents of transmission for potentially pathological fungi.
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Fungi isolated in school buildings

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The aim of the study was to determine the species composition of fungi occurring on wall surfaces and in the air in school buildings. Fungi isolated from the air using the sedimentation method and from the walls using the surface swab technique constituted the study material. Types of finish materials on wall surfaces were identified and used in the analysis. Samples were collected in selected areas in two schools: classrooms, corridors, men’s toilets and women’s toilets, cloakrooms, sports changing rooms and shower. Examinations were conducted in May 2005 after the heating season was over. Fungi were incubated on Czapek-Dox medium at three parallel temperatures: 25, 37 and 40°C, for at least three weeks. A total of 379 isolates of fungi belonging to 32 genera of moulds, yeasts and yeast-like fungi were obtained from 321 samples in the school environment. The following genera were isolated most frequently: Aspergillus, Penicillium and Cladosporoium. Of the 72 determined species, Cladosporium herbarum, Aspergillus fumigatus and Penicillium chrysogenumoccurred most frequently in the school buildings. Wall surfaces were characterised by an increased prevalence of mycobiota in comparison with the air in the buildings, with a slightly greater species diversity. A certain species specificity for rough and smooth wall surfaces was demonstrated. Fungi of the genera Cladosporium andEmericellawith large spores adhered better to smooth surfaces while those of the genus Aspergilluswith smaller conidia adhered better to rough surfaces. The application of three incubation temperatures helped provide a fuller picture of the mycobiota in the school environment.
School buildings typically serve a number of functions simultaneously, owing to which they seem to be especially exposed to the expansion of fungi with wide enzymatic capabilities, including fungi of the genus Penicillium. In total, 34 isolates belonging to 20 species were obtained within two schools. The most frequently isolated species included: Penicillium chrysogenum, P. waksmanii, and P. citrinum. Over 65% more fungi were isolated in the heating season than when the heating was off. This period was also characterized by an almost threefold greater taxonomic spectrum. Especially in the heating season, the prevalence and species spectrum of the fungi of the Penicillium genus in school buildings should be constantly monitored due to their environmental plasticity as well as toxigenic, allergenic and antibiotic properties.
The study is part of hydromycological investigations of man-affected water bodies in Olsztyn. Our results show that yeast-like fungi are permanent components in three selected lakes (lakes Tursko, Długie and Skanda). Their abundance and species composition depend on the trophic state of each lake.
The objective of this research was to determine the species composition of school rooms of various functionalities, the selection of typical species, and the development of assumptions of school environment monitoring. Out of the 151 species identified in the studied school rooms, 52 were grown at a temperature above 25ºC, which constituted 34.4%. Seven species were isolated by incubating the samples at 40ºC, yet it was indispensable only for Acremonium alabamense. Thermophilic fungi constituted 55.3% of the species with the above-indicated Bio-Safety Level status. Out of the identified fungi, one was classified to bio-safety class (Blastomyces dermatitidis), whereas 22 species were claccified as BSL2 (most were: Aspergillus funigatus, A. flavus, A. terreus) and 61 species to class 1. So far, investigations of the indoor bio-aerosol of rooms in this part of Europe have not demonstrated such a wide species spectrum of fungi, as is the case in this study.
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh. glutinis, Rh. minuta, Rh. mucilaginosa and two-species isolates: Rh. minuta + Candida dubliniensis, Rh. mucilaginosa + C. albicans and Rh. mucilaginosa + Saccharomyces cerevisiae. The enzymatic activity of the isolated fungi was evaluated as high and very high in the case of acidic and alkaline phosphatase, esterase lipase, lipase, as well as leucine, valine and cysteine arylamidase. The highest values were determined in Rh. mucilaginosa with co-occurrence of C. albicans and Rh. minuta with co-occurrence of C. dubliniensis. In the ontosphere of oncologic patients, the increasingly frequent appearance of the species of fungi with high and very high enzymatic activity indicates the progressing risk posed by opportunistic forms, which includes Rhodotorula species.
A total of 69 isolates of yeasts were recorded in the indoor air of the school buildings: 43 in heated rooms and 26 in unheated rooms. Perfect stages prevailed. Fungi isolated in our study belonged to 39 species. These were mostly monospecific isolates although five two-species isolates were noted. Differences in the properties of physiological characters of fungi isolated in both study seasons were observed. As indoor and outdoor air does not mix during the heating season, a specific substrate for prototrophic, non-fermenting yeastlike fungi forms. Acid production allows fungi to dissolve inorganic compounds in building structures and to release needed microcomponents. Abilities to produce carotenoid pigments are clearly promoted in yeast-like fungi living indoor. This may be related to the accumulation of compounds that are indirect stages in the cycle of biosynthesis of carotenoids or a surplus of oxidizing compounds.
This work provides a morphological description of a parasitic fungus Puccinia passerinii Schroet. discovered in the Biebrza National Park on bractless toadflax Thesium ebracteatum Hayne, a host plant listed in Appendix II of the Habitat Directive and strictly protected in Poland. Puccinia passerinii was recorded in 1916 within the borders of current-day Poland, however, owing to a lack of herbarial materials it was not included into the checklist of Polish microscopic fungi. This work is the first report, after 100 years, on the appearance of this species in Poland, which enlarges the list of microfungi in this country and particularly in the Biebrza National Park. Moreover, a common parasite of rust fungi – Sphaerellopsis filum (Biv.) B. Sutton is reported for the first time on P. passerinii.
A total of 111 isolates of yeast-like fungi and yeasts belonging to 40 species of 19 genera were identified in indoor air and outdoor air. Only one species, Kluyveromyces marxianus, was recorded in both types of air and seasons (spring and autumn). Kluyveromyces lactis and Yarrowia lipolytica, a species having the greatest symbiotic abilities, dominated in indoor air and outdoor air, respectively. Intensely used rooms, especially those with limited access of air, have the broadest range of species of yeast-like fungi. A comparison of both habitats shows that school rooms pose a greater epidemiological risk of yeast-like infections than outdoor air. The indoor as well as outdoor mycobiota undergoes phenological changes although it is determined by other biotic and abiotic factors.
Fungi of the genus Trichosporon are widespread in the biosphere and are a frequent component of the skin mycobiota. They are opportunistic organisms that adopt a commensal lifestyle on the skin in healthy humans but can cause pathological changes (trichosporonosis) of various intensity and range in immunocompromised persons. The species recorded in our study (T. asahii, T. capitatum, T. cutaneum, T. inkin, T. pullulans) are potential anthropopathogens. T. asahii, T. cutaneum and T. inkin are classed as BSL-2 in the biosafety classification.
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