DREAM is a Ca2 -dependent transcriptional repressor that regulates the expression of multiple genes, some of which are implicated in pain mechanisms. To further investigate the role of DREAM in pain we have developed transgenic lines of mice overexpressing dominant active mutants of DREAM (daDREAM). They were tested for thermal and mechanical sensitivity in basal conditions and following CFA-induced infl ammation or CCI-induced neuropathic pain. Naïve transgenic (TG) mice showed signifi cantly higher responses to noxious heat as compared to wild type (WT). In a visceral pain model,TG animals were markedly hyperalgesic in comparison to WT. In models of chronic infl ammation (CFA), TG animals show a delay in the development of thermal hypersensitivity as compared to the WT animals. In a model of neuropathic pain (CCI), within 5 days post op, TG animals were as hyperalgesic as WT animals. However, considering that baseline sensitivity was lower in TG animals, the degree of hyperalgesia was less than that of WT animals when compared to pre-operative values. Thus, daDREAM is implicated in various pain states as well as baseline noxious sensitivity.The behavioural data will be supplemented with histochemical data characterising sensory neurone populations in both groups of animals.
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