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1
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Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced by lysophosphatidylcholine

100%
Park S., Kim J. A., Choi S., Suh S. A.
Journal of Physiology and Pharmacology
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2010
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tom 61
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nr 4
375-381
Oxidative stress in the vascular wall has intimately been implicated in the apoptosis of human umbilical vein endothelial cells (HUVECs) by lysophosphatidylcholine (LPC). However, the major type of reactive oxygen species (ROS) in this apoptotic signaling pathway remains to be clarified. In this study, we report that superoxide mediate LPC-induced caspase-3 dependent apoptosis in cultured HUVECs. The stimulation of HUVECs with LPC evoked apoptosis and ROS generation, and inhibited nitric oxide (NO) production in a dose-dependent manner. The classical caspase-3 dependent apoptosis was determined after 16 hours treatment by Western blotting using an antibody against cleaved caspase-3. The caspase-3 activation induced by LPC was prominently inhibited by antioxidants or NO donors and enhanced by NO inhibitors. Especially, LPC-induced caspase-3 activation was inhibited by superoxide dismutase (SOD) and enhanced by ammonium tetrathiomolybdate, SOD inhibitor. Additionally, xanthine/xanthine oxidase mixture increased the caspase-3 activation but catalase failed to reduce this superoxide-induced caspase-3 activation. These findings indicate that the superoxide generation caused by LPC activates the caspase-3 which results in HUVECs death. This study reveals some evidences linking superoxide with caspase-3 activation and provides a new dimension to superoxide-mediated caspase-3 activation in developing the endothelial dysfunction and atherosclerosis.
2
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Effect of thiol antioxidants on lipopolysaccharide-induced cyclooxygenase-2 expression in pulmonary epithelial cells

100%
Choi S., Lim J. W., Kim H.
Journal of Physiology and Pharmacology
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2018
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tom 69
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nr 4
3

Expression profile analysis of human peripheral blood mononuclear cells in response to aspirin

100%
Choi S., Park H. S., Cheon M. S., Lee K.
Archivum Immunologiae et Therapiae Experimentalis
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2005
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tom 53
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nr 2
4
Content available remote

Stimulation of large-conductance Ca2plus-activated Kplus channels by the Naplus-Ca2plus exchanger inhibitor dichlorobenzamil in cultured human umbilical vein endothelial cells and mouse aortic smooth muscle cells

88%
Liang G. H., Park S., Kim J. A., Choi S., Suh S. H.
Journal of Physiology and Pharmacology
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2009
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tom 60
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nr 1
43-50
We investigated the effects of the selective inhibitor of Na+/Ca2+ exchanger (NCX), 2',4'- and 3',4'-dichlorobenzamil (DCB), on large-conductance Ca2+-activated K+ (BKCa) channels in cultured human umbilical vein endothelial cells (HUVECs) and fresh isolated mouse aortic smooth muscle cells (MASMCs) using the patch clamp techniques. Both kinds of DCB reversibly activated BKCa currents in whole-cell clamped HUVECs or MASMCs. The EC50 of 2',4'-DCB for BKCa current activation in HUVECs was 2.64 ± 0.10 µM. In inside-out and outside-out patches, 2',4'-DCB remarkably increased BKCa channels activity. 2',4'-DCB increased open frequency, but had no significant effect on mean open time. In inside-out patches, 2',4'-DCB shifted the relationship curve between [Ca2+]i and open probability (NPo) to the left; the [Ca2+]i required to evoke half-maximal activation changed from 1087.45 ± 142.91 nM to 500.24 ± 66.83 nM by 10 µM 2',4'-DCB. In addition, 2',4'-DCB shifted the relationship curve between membrane potential and NPo to the left; the membrane potential to evoke half-maximal activation changed from 81.1 ± 2.4 to 64.7 ± 3.1 mV by 10 µM 2',4'-DCB. 3',4'-DCB also increased BKCa channels activity. There was no significant difference in the effect of DCB on BKCa channels between both excised patches. These results suggested that 2',4'- and 3',4'-DCB activate BKCa channels activity in HUVECs and MASMCs by increasing the sensitivity of BKCa channels to cytosolic free Ca2+ and membrane potential. Our report would provide a consideration if they are used as NCX blocker in vascular endothelial cells or smooth muscle cells.
5

Effects of functional rice cake on the loperamide-induced constipation in rats

76%
Choi H., Park J. O., Choi S., Yoon S., Park H., Kim J.
Polish Journal of Food and Nutrition Sciences
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2011
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tom 61
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nr Suppl.1
6

Expression analysis of D-type cyclin in potato (Solanum tuberosum L.) under different culture conditions

64%
Rhee Y., Hwang K. R., Cho S., Lee M., Kil E.-J., Choi S., Hahn B.-S., Kim D., Auh C.-K., Lee S.
Acta Physiologiae Plantarum
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2016
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tom 38
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nr 02
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