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1

Conditions for dormancy breaking and germination of European hophornbeam [Ostrya carpinifolia Scop.] seeds. Part II

100%
Chmielarz P.
Arboretum Kórnickie
|
1992
|
tom 37
113-124
2

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

100%
Chmielarz P.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 3
To evaluate the feasibility of long-term cryopreservation of Polish provenances of silver birch (Betula pendula), the sensitivity of conditionally dormant seeds to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196℃) was evaluated. The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or for 2 years in LN was also determined. Germination tests revealed no critical WC for seeds [to 0.02 g H₂O g⁻¹ dry mass (g g⁻¹)]. Seeds tolerated freezing in LN within specific safe range of WC 0.02–0.23 g g⁻¹ (nuts). Seeds desiccated to the safe WC and stored in LN for 2 years had similar or higher germination as seeds stored at -3℃ for 2 years, depending on provenance. Therefore, long-term cryopreservation of B. pendula seeds in gene banks is feasible.
3
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Odpornosc mrozowa zoledzi debu szypulkowego [Quercus robur L.] o wilgotnosci 42 procent

100%
Chmielarz P.
Sylwan
|
1996
|
tom 140
|
nr 08
33-41
4

Mozliwosci zastosowania kriogeniki w przechowywaniu nasion - metody, technologie, urzadzenia i sprzet

100%
Chmielarz P.
Postępy Techniki w Leśnictwie
|
2002
|
tom 82
47-52
5

Cryopreservation of Pinus sylvestris seeds for three years

100%
Chmielarz P.
Arboretum Kórnickie
|
1998
|
tom 43
37-42
6

Immature stage of Sibinia pellucens [Scopoli, 1772] [Coleoptera: Curculionidae]

100%
Chmielarz P.
Annales Zoologici
|
1997
|
tom 47
|
nr 1-2
Larva and pupa of Sibinia pellucens are described in detail. The larvae were collected from fruits of Melandrium album (Caryophyllaceae); some larvae were reared till pupation.
7
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Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen

100%
Chmielarz P.
Dendrobiology
|
2002
|
tom 47 Supplement
The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.
8

Immature stages and bionomics of Amalorrhynchus melanarius [Stephens, 1831] [Coleoptera: Curculionidae: Ceutorrhynchinae]

100%
Chmielarz P.
Genus. International Journal of Invertebrate Taxonomy
|
1995
|
tom 06
|
nr 3-4
9
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Zachowanie różnorodności genetycznej dębu szypułkowego

88%
Chmielarz P.
Sylwan
|
1994
|
tom 138
|
nr 03
67-70
10

Induction and proliferation of embryogenic tissues of Serbian spruce (Picea omorika) and their maintenance in liquid nitrogen

51%
Hazubska-Przybyl T., Chmielarz P., Michalak M., Bojarczuk K.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
11
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Potential possibilities of the induction of European beech (Fagus sylvatica ) tissue cultures from various types of explants

51%
Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
12
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In vitro responses of various explants of Fagus sylvatica

51%
Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.
Dendrobiology
|
2015
|
tom 73
There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
13
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Czy kriokonserwacja jest bezpieczna? Określenie potencjalnych zmian w tkankach roślinnych poddanych kriogenicznemu przechowywaniu

51%
Michalak M., Plitta B. P., Kotlarski S., Chmielarz P.
Sylwan
|
2014
|
tom 158
|
nr 10
Cryopreservation (storage in liquid nitrogen, –196°C) is a technique that ensures safe, long−term conservation of plant species with recalcitrant seeds, vegetatively propagated species and biotechnology products such as somatic embryos, cell lines and genetically transformed material. The aim of cryostorage is to keep plant tissue in a safe way, which does not cause changes during storage at neither phenotypic, cytological and histological, biochemical, genetic nor epigenetic level. Therefore, before cryostorage will be applied for long−term preservation it should be thoroughly assess if it does not cause any injuries in plant material.
14
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Kriogeniczne przechowywanie nasion

51%
Plitta B. P., Michalak M., Kotlarski S., Chmielarz P.
Sylwan
|
2013
|
tom 157
|
nr 10
Cryopreservation is a method of storage of biological material at the temperature of liquid nitrogen (–196°C, LN). The main advantage of this method is the possibility to store viable cells for a long time. Desiccation and freezing sensitivity of seeds, their fragments or other plant organs, which are useful as genetic resources, should be investigated before cryopreservation.
15

Prolonged incubation with desipramine differentially modulate A1A-and A1B-adrenoreceptor signaling in PC12 cells

51%
Chmielarz P., Kowalska M., Rafa-Zablocka K., Nalepa I.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Action of tricyclic antidepressant drugs (TCA) involves inhibition of noradrenaline re-uptake, however, several of TCA also exhibit affinities for adrenergic receptors (α1-ARs). Furthermore there are reports in literature implying role of α1-AR in depression and antidepressant action. However due to scarcity of selective ligands discriminating the α1-ARs, the specific role of each of three subtypes remains elusive. Aim of current study was to investigate if and how prolonged incubation with desipramine (DMI), a TCA exhibiting affinity for α1-AR could affect the α1A- and α1B-adrenoreceptor reactiveness in vitro. METHODS: Measurements were performed in PC12 cells stably transfected with human α1A- and α1B‑AR. To assess receptor activity cells expressing either one of the receptors were incubated for 24 hours with 10 µM DMI or vehicle. After incubation receptor responsiveness to agonist was assayed by stimulation with serial dilutions of noradrenaline (form 10-9 to 10-4 M) and measurement of inositol phosphate generation with use of TR-FRET based assay. RESULTS: The 24 hours preincubation with DMI shifted the noradrenaline dose response curve rightwards in case of both α1A-AR (EC50 = 0.9 µM and EC50 = 14.5 µM for VEH and DMI preincubated cells, respectively) and α1B‑AR (EC50 = 0.5 µM and EC50 = 2.7 µM, for VEH and DMI preincubated cells, respectively). The effect of DMI was more pronounced for α1A-AR and difference between receptor subtypes was reflected in significant receptor×treatment interaction (P<0.001) in two-way ANOVA comparison. CONCLUSIONS: Our data indicate that DMI can differentially modulate activity of α1A- and α1B‑AR. The diverse susceptibility of α1A- and α1B‑AR to DMI action may be interesting in the light of reports of different role of these receptors in depressive-like behaviors in mice. This study was supported by statutory funds of Institute of Pharmacology, PAS.
16
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Klonowanie najstarszych dębów pomnikowych rosnących w Polsce z wykorzystaniem metody in vitro

51%
Kotlarski S., Michalak M., Chmielarz P.
Rocznik Polskiego Towarzystwa Dendrologicznego
|
2019
|
tom 67
17

The lack of glucocorticoid receptor on noradrenergic cells does not influence an inflammatory response after chronic stress

45%
Kusmierczyk J., Chmielarz P., Trojan E., Roman A., Nalepa I.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Noradrenergic neurons with terminals in the hypothalamus are known to regulate activity of the hypothalamic pituitary adrenal (HPA) axis. The aim of the study was to evaluate whether glucocorticoid receptor (GR) ablation in noradrenergic cells affects the inflammatory response in the central nervous system (CNS) and functioning of immune organs under chronic restraint stress (CIS) conditions. METHODS: Selective ablation of GRs in the noradrenergic system (GRDBHCre mice) was achieved using the Cre/loxP approach. The male mice were kept in standard laboratory conditions. The CIS procedure was performed by placing animals, for 2 hours daily, in 50 ml disposable centrifuge tubes and was repeated for 14 days The expression of cytokines in selected brain structure was analyzed with TaqMan RT-PCR assay. The relative thymus and spleen mass were calculated as well as peritoneal cell ability to production of selected cytokines after stimulation. RESULTS: We found that CIS procedure caused the decrease in body and relative thymus weights in both wt and mutant mice. The mRNA expression of interferon gamma and interleukin-6 genes was elevated in the hypothalamus, prefrontal cortex and hippocampus in mutation independent manner. We also found the increase in production by peritoneal macrophage cells on tumor necrosis factor alpha and interleukin 1beta after pro-inflammatory stimulation and increase in interleukin 4 productions in anti-inflammatory stimulation in both wt and mutant mice. CONCLUSIONS: The regulation of inflammatory process is a complex process in which a number of cells and molecules play different roles in a coordinated and well-controlled manner. Therefore, the lack of GR in noradrenergic cells might be too subtle and insufficient modification to cause disturbances in inflammatory responses after chronic stress. Supported by grant no. POIG.01.01.02-12-004/09 co-financed by the ERDF and by statutory funds of Institute of Pharmacology PAS.
18
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Effects of spermidine on germination of Salix spp. after storage under controlled conditions

45%
Wawrzyniak M. K., Ley-Lopez J. M., Kijowska-Oberc J., Chmielarz P., Ratajczak E.
Dendrobiology
|
2022
|
tom 87
19

The influence of conditional inactivation of GR in noradrenergic system on stress related behavior in mice

39%
Kreiner G., Chmielarz P., Kusmierczyk J., Parlato R., Schuetz G., Nalepa I.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Depression is a mental disease affecting complex cognitive and emotional functions. Stress induced hyperactivity of hypothalamic-pituitary-adrenal system (HPA) is believed to be one of the major contributors to its pathology. The activity of HPA is controlled by glucocorticoid receptors (GR) which function may be impaired in depression, resulting in reduced GR-mediated negative feedback on the HPA-axis. Most of the compounds which modulate GR action also influence noradrenergic system by increasing noradreneline levels. The aim of this study was to investigate if conditional inactivation of GR in noradrenergic neurons of mice affects the animal behavior in stressful conditions. Selective ablation of GR in noradrenergic system was achieved using the Cre/loxP approach by crossing transgenic mice hosting the Cre recombinase under the dopamine beta-hydroxylase (DBH) promoter with animals harboring the floxed GR gene. Resulting GRDBHCre mutant mice were born at expected rates, viable and showed no obvious physical impairment regarding life span, weight gain and locomotor activity. Also plasma cortisol levels did not differ between mutant and control mice. Animals were screened for anxiety and depressive-like behavior in light/ dark box test (LDT) and tail suspension test (TST). Male mutant mice did not unveil any differences from their control littermates in basal state nor after acute restraint stress (2 hrs). However, both tests performed after chronic restraint stress (14 days, 2 hrs/day) revealed that GRDBHCre mice were resistant to this type of experimental procedure showing similar anxiety status and immobility time as non-stressed controls. Our mutant mice may represent an interesting tool to study the role of stress in depression in context of noradrenergic system which is important target for antidepressant therapy. This study was supported by grant POIG.01.01.02-12-004/09 (DeMeTer) financed by European Regional Development Fund.
20

Możliwość gromadzenia leśnych zasobów genowych w warunkach ex situ

33%
Chmielarz P., Suszka J., Bujarska-Borkowska B., Lewandowski A., Wawrzyniak M., Kotlarski S., Hazubska-Przybyl T., Litkowiec M., Plitta-Michalak B., Michalak M.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2018
|
nr 283
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