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A methicillin-resistant Staphylococcus aureus (MRSA) referred to as livestock-associated (LA-MRSA) has recently emerged in farm animals, particularly in pigs. Strains of this MRSA variant from Europe and North America mostly belong to clonal complex (CC) 398. Generally LA-MRSA cause asymptomatic colonization among pigs, but also in veal calves, broiler chickens, turkeys, and horses. People in contact with livestock animals are at high risk of asymptomatic colonization or infection with these bacteria. In previous years, the impact of LA-MRSA on human health was considered small. However, LA-MRSA has become more prevalent among people without direct livestock contact, especially in areas with a high density of pig production. As a result of horizontal gene transfer S. aureus CC398 strains are constantly evolving. The adaptive power of S. aureus to new hosts and acquisition of resistance to antibiotics may cause the emergence of new, more virulent clones. LA-MRSA has evolved from human-adapted methicillin-susceptible S. aureus CC398, which was proved by comparative genome analysis. The adaptation to livestock was associated with several genetic changes. The most worrying aspect of MRSA CC398 seems to be its ability to spread to humans. For this reason, continuous surveillance of further genetic changes is recommended.
The aim of the study was to evaluate the incidence of Lawsonia intracellularis in a population of healthy pigs and to determine the section of ileum most frequently inhabited by the microorganism. The investigation covered a total number of 832 healthy finishing pigs of similar age. The body weight of individuals ranged from 90 to 120 kg. The animals did not show any symptoms of the disease and they were directed to slaughter. Samples of ileum, caecum and colon were subjected to nested PCR test for the presence of L. intracellularis. The results showed that the bacteria mainly inhabits the ileum (33.3%). The p78 genome fragment of L. intracellularis was sporadically detected in the large intestine: 8.2% in colon and 5.4% in caecum samples. This specific fragment was found in 43.1% of intestinal samples of healthy slaughter pigs from different regions of Poland. The percentage of the herds infected was established at 73.4%.
Antimicrobial resistance (AMR) is a global public health issue. Multidrug resistance (MDR) genes that confer resistance to antimicrobials from different classes are of particular importance in the spread of AMR. Moreover, some of these MDR genes are involved in resistance to critically important antimicrobial agents used in human and veterinary medicine. Staphylococci isolated from animals and humans harbor a wide range of resistance genes, including MDR genes. Location of MDR genes on mobile genetic elements facilitate the exchange of these genes between staphylococci of animal and human origin. The emergence of resistant Staphylococcus spp. is probably linked to therapeutic or prophylactic antimicrobial use through not only direct selection of the corresponding resistance, but also indirect selections via cross-resistance and co-resistance. Judicious use of antibiotics and the knowledge of the genetics of MRD genes and other resistance genes is indispensable to counteract further dissemination of staphylococcal MDR genes.
Equine proliferative enteropathy (EPE) is an emerging enteric disease of foals caused by Lawsonia intracellularis. The disease has been reported worldwide. Affected foals display lethargy, weight loss, peripheral edema, diarrhea and colic. An antemortem diagnosis of EPE is based on clinical signs, the presence of hypoproteinemia, abdominal ultrasonography, positive serology and molecular detection of L. intracellularis in feces. The transmission of L. intracellularis, as well as immunological and epidemiological aspects of the disease within a herd have remained poorly investigated. The results of the latest investigations demonstrate that a primary role in immunity is played by IgA mucous antibodies and cell immunity. To attain a full knowledge of these defense mechanisms further research is necessary. The disease is considered a zoonosis.
In hospitals, veterinary clinics and food processing plants, infectious agents have emerged that are increasingly resistant to applied drugs and disinfection procedures. Therefore, at present it is even more vital to develop and implement new, more effective methods of their inactivation. An example of the most recent solutions in this field is the application of photocatalysis. Among those processes of photocatalysis that have been most studied in the context of their ability to eradicate viruses, prions, bacteria and moulds are TiO₂/UV, ZnO/UV and MgO/UV, where, respectively, titanium dioxide (TiO₂), zinc oxide (ZnO) and magnesium oxide (MgO) are used as photocatalysts after they have been powdered into nanoparticles (NPs), whereby the ultraviolet radiation (UV) is used as an agent generating free radicals. Nano-sized oxides of titanium, zinc and magnesium are applied to create thin photocatalytic films covering various surfaces that thereby display self-disinfecting properties. The susceptibility of infectious agents to photocatalytic processes presents the following order: (viruses = prions) > gram-negative bacteria > gram-positive bacteria > yeasts > moulds. In the light of the most recent studies, photocatalysis seems to be a very promising tool to help overcome problems related to hygiene and public health protection.
Easy-to-clean and easy-to-disinfect surfaces play a key role in keeping the right sanitary and hygienic standards in hospitals, veterinary clinics and food processing plants. In recent years, due to the development of nanotechnology, self-cleaning and self-disinfecting surfaces are applied on an increasing scale. They are covered by photocatalytic surfaces made of nanomaterials (NMs) on which, upon exposure to the ultraviolet radiation, advanced oxidation processes (AOPs) can take place. Those processes present an effective method of bacteria eradication. One of the most studied AOPs in relation to microorganism eradication is the TiO₂/ UV-process, where titanium dioxide (TiO₂) is used as a photocatalyst, and the ultraviolet radiation (UV) is used as an agent generating free radicals. The high efficiency of the gram-negative and gram-positive bacteria inactivation by the methods using photocatalytic properties of nano-sized TiO₂ (nano-TiO₂) contributes towards its increasing application. Disinfection based on free radicals reactions is considered to become one of the most promising tools to solve difficult problems related to hygiene and public health protection in the near future.
Every year, several millions of people all over the world are diagnosed with cancer. Despite the tremendous development of medical sciences, a remarkable number of people die due to late diagnosis or ineffective cancer therapy. Since most of the tumors are highly resistant to drugs, research for new effective therapy methods is continuing. Great expectations for a breakthrough in the fight against cancer are attributed to nanotechnology. A new interdisciplinary field of science dealing with the creation of nanoparticles (NPs) and nanomaterials (NMs) that are variously applied, e.g. in nanomedicine. NPs and NMs have gained an increased consideration in cancer therapy in recent years, performing as carriers of medicine, as well as photo- or sonosensitisers, compounds generating reactive oxygen species (ROS) formed by ultraviolet light (UV) excitation or ultrasound (US) activation, respectively. Targeted therapy is based upon the attachment of specific ligands or antibodies to nanoparticles. This process guarantees not only increased therapy efficiency, but it also lowers the cytoand genotoxicity of the active compound towards the healthy cells. Nano-sized titanium dioxide (nano-TiO2) presents an example of a substance with an increasing role in the eradication of tumor cells. Currently, studies are conducted to examine its application, i.e. in the cancer fighting photo- and sonodynamic therapies.
Veterinary students taking part in practical classes on “Hygiene of food of animal origin” course were tested on taste and odor identification as well as color differentiation. The results were subjected to statistical analysis to: (a) evaluate the influence of sex, smoking, time of day and status of health declared by the students on the results of tests; (b) determine the number of individuals who may fail the tests on taste and odor identification as well as color differentiation; (c) evaluate whether the sensory acuity of veterinary students changed in the 40 years of observation. The study involved 3310 students. A test for taste and odor identification was performed according to Polish Standard (PN-65/A-04021). The test for color differentiation was carried out using Rabkin color plates. The results of the study were elaborated using IBM SPSS Statistics 23. The chi-square test was used to determine whether there are significant differences between numbers of individuals assessed positively in particular experimental groups. The significance of the effect of experimental factors on the number of correctly identified samples was determined using analysis of variance. It was found that sex and smoking exerted a statistically significant effect (p < 0.01) on the number of subjects assessed positively and the number of correctly identified samples in three conducted tests. Female students more often than male students were assessed positively and correctly identified larger number of samples in all conducted tests. Nonsmokers obtained better results than smokers in all performed comparisons (significant difference at p < 0.01). It is worth noting that only 65.9% of students passed all three tests, and 1.3% of them did not pass any. In this last group were only men. The analysis of variance indicated that as time passed (since 1975 to 2015) sensory acuity of veterinary medicine students has increased.
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