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Decomposition rates and changes of carbon and nitrogen contents of Sphagnum litter in mire vegetation can help to explore the ecological effects of climate change and the role of environmental factors from a local to an ecosystem scale. The objective of the study was to determine the relationship between mentioned parameters in small and isolated Sphagnum dominated mires. Measurements had been conducted throughout a year by placing litterbags filled with Sphagnum biomass in three vegetation types (open peat bog, poor fen, alder carr) of a mire ecosystem in Hungary. Peat decomposition rates differed to a great extent; the slowest decomposition rate (39.1±9.52%) was in the alder carr, indicating that slower decomposition could be characteristic for this kind of vegetation type of mire. Between Sphagnum dominated microhabitats, open peat bog showed medium (65.57±4.05) while poor fen the fastest (68.61±5.5) rates in decomposition. The C/N ratio of the Sphagnum litter showed significant decrease (P <0.005) in all studied micro-environments. Slower N release was observed from litter of Alnus dominated association (31.3±6.9%) compared to Sphagnum dominated ones (56.5±8.3%). Our findings showed that the decomposition rates were more dependent on vegetation type than C/N ratio and this relationship was also revealed at a small spatial scale.
Foraminiferal assemblages from the neritic environment reveal the palaeoecological impact of nutrient types in relation to shore distance and sedimentary setting. Comparatively proximal siliciclastic settings from the Boreal Domain (Brora section, Eastern Scotland) were dominated by inner−shelf primary production in the water column or in sea bottom, while in relatively seawards mixed carbonate−siliciclastic settings from the Western Tethys (Prebetic, Southern Spain), nutrients mainly derived from the inner−shelf source. In both settings, benthic foraminiferal assemblages increased in diversity and proportion of epifauna from eutrophic to oligotrophic conditions. The proximal setting example (Brora Brick Clay Mb.) corresponds to Callovian offshore shelf deposits with a high primary productivity, bottom accumulation of organic matter, and a reduced sedimentation rate for siliciclastics. Eutrophic conditions favoured some infaunal foraminifera. Lately, inner shelf to shoreface transition areas (Fascally Siltstone Mb.), show higher sedimentation rates and turbidity, reducing euphotic−zone range depths and primary production, and then deposits with a lower organic matter content (high−mesotrophic conditions). This determined less agglutinated infaunal foraminifera content and increasing calcitic and aragonitic epifauna, and calcitic opportunists (i.e., Lenticulina). The comparatively distal setting of the Oxfordian example (Prebetic) corresponds to: (i) outer−shelf areas with lower nutrient input (relative oligotrophy) and organic matter accumulation on comparatively firmer substrates (lumpy lithofacies group) showing dominance of calcitic epifaunal foraminifera, and (ii) mid−shelf areas with a higher sedimentation rate and nutrient influx (low−mesotrophic conditions) favouring potentially deep infaunal foraminifers in comparatively unconsolidated and nutrient−rich substrates controlled by instable redox boundary (marl−limestone rhythmite lithofacies).
Six green pea varieties (Pisum sativum L.) with different maturity and leaf type were grown under three irrigation conditions (irrigated, water deficit, non-irrigated) in dry seasons with the objective to examine the relationship among stomatal resistance, stomatal density, chlorophyll content expressed by SPAD value, normalized difference vegetation index (NDVI) and the yield during the growing season. During flowering and pod ripening, significant difference was found in stomatal resistance between the mid- and late-ripening varieties. Those varieties had high stomatal resistance during flowering and pod development produced low yield under water deficiency. In drought under non-irrigated conditions, close significant correlations were found between stomatal resistance and SPAD, as well as NDVI measured during flowering and the yield and water use efficiency for green seeds (WUEy). Under this condition, when the stomatal resistance does not exceed 3 s cm-1 during green pea flowering, 4.3 t ha-1 of yield can be expected. In a dry season, under water deficiency, the SPAD values can be considered drought stress marker and it is more appropriate for evaluation of water use ability and productivity of pea varieties than the extent of stomatal resistance. The results can be used for the selection of pea genotypes adapted to water deficit under field conditions.
The aim of this study was the comparison of growth parameters, yields and qualitative properties of muscles in broiler hybrids ISA 220 and intermediate type ISA 257. One hundred and one one-day-old chicks, comprising approximately equal numbers of males and females were used. During the experiment the feed consumption (per 1 kg of body weight gain), weight gains, and body weight were recorded. After the processing of the broiler chickens, the weight of carcass, and yields (of the whole carcass, breast muscle, thighs, boned thigh muscles, wings, skeleton) were recorded. The breast and thigh muscles were analyzed for the content of nitrogen compounds, fat, water, amino acid, and weight losses during the cooking. The sensory properties of breast and thigh muscles of both groups were compared. The mean body weight of the broiler chickens at the beginning of the experiment was 43 g (ISA 257), and 36 g (ISA 220) respectively. On day 45, the mean body weights and carcasses were significantly higher (p < 0.05) in group ISA 220 (2507 g and 1676 g respectively) in comparison with ISA 257 (1890 g and 1249 g). These results corresponded with the results of carcass yields. The mean yield of the whole carcass was significantly higher (p < 0.05) in ISA 220 (66.64%) in comparison with ISA 257 (66.06%). However, the mean yields of thighs, boned thigh muscle, and wings, which are considered as ideal parts, for grilling were significantly higher (p < 0.05) in ISA 257. Higher differences were recorded in fat and water content. The hybrids ISA 220 contained a higher amount of water in breast and thigh meat, and fat content was higher in meat of ISA 257, mainly in breast meat. The results of sensory examination showed better acceptability of ISA 257 hybrid than ISA 220.
Blood serum concentrations and biological half-life of oxytetracycline (OTC) after long action preparations were investigated in adult sheep of the Slovak Merino breed. A the same time their local tolerance has also been observed. In the first group oxytetracycline was administered in the form of preparation Tetraxyl L. A. inj. a.u.v. and in the second group in the form of preparations Alamycin L.A. inj. a.u.v. The blood serum concentrations of OTC were studied at intervals of 1, 3, 6, 24 hours and 2, 3, 4 and 5 days after single administration of the preparations. Oxytetracycline has been determined by high HPLC chromatography. Therapeutically concentrations of OTC (above 0.5 ?g.ml-1) produced by Tetraxyl were detected within 74 hours and after Alamycin administration within 67?68 hours. Detectable concentrations of OTC (under MIC) of Tetraxyl (0.28 ?g.ml-1) and Alamycin (0.14 ?g.ml-1) were recorded in 96 hours. On the 5th day all samples in both groups were negative. Biological half-life of Tetraxyl was determined at 36.5?38.5 hours and Alamycin at 36.0?37.0 hours. Short-time palpation hyperaesthesia was recorded in 1 sheep (within 13 hours) after Tetraxyl administration and in 2 sheep (within 15 hours) from the Alamycin group. On the basis of the results it was concluded that in selected pharmaceutically parameters and also in local tolerance Tetraxyl L.A. is more favorable in comparison with Alamycin L.A.
Irrigation is one of the most important factors of surface water resources management, therefore water saving irrigation farming is desired which could promote better utilisation of the agricultural potential of Hungary. In our article we deal with the situation, problems and development possibilities of water resources management, especially with the development possibilities of irrigation farming.
Bioprotective lactic acid from bacterial culture FloraCarn L-2u was tested for its biopreservative capacities to improve the quality and safety of cod salad, with additives: NaCl, acetic acid, sugar, mayonnaise, mustard and vegetables. The salad was divided into three portions: one untreated and two treated with a commercial Lactobacillus alimentarius biopreservative, inoculated in different concentrations (0.05 and 0.1g/kg). Lactic acid production, pH, development of the indigenous flora were examined for 14 days of aerobic storage in a refrigerator. The level of indigenous coliform bacteria, Staphylococcus aureus and moulds were reduced. The antibacterial effect of FloraCarn L-2u is attributed to lactic acid produced by this culture.
After intramuscular administration to laying hens, the highest mean concentration of oxytetracycline in (OTC) egg white was noticed on the 1st day after treatment (the 6th day of the experiment). On the 6 th day after treatment OTC was not detected. In the egg yolk, the highest OTC concentration was observed on the 3rd day after treatment. On the 13 th day after treatment no residues of oxytetracycline were detected above the detection limit of the used method (50 ppb). After oral administration, OTC residues appeared in the egg white as early as the first day after treatment began. The residues were detected until the 9th day after treatment. In the yolk, residues appeared four days after the beginning of the treatment and they were detected until the 12th day after treatment.
A new stand, formed by some fifty individuals of Ambrosia artemisiifolia L., an invasive plant of alien origin, was discovered at the Katowice railway station (southern Poland), in 2011. Two years later, the stand (increased to more than 160 individuals in the meantime) was re-visited, phytosociological survey was made, and four soil cores (5 cm deep and 80 cm² surface area, each) were taken for seed bank studies. The phytosociological sampling revealed 28 co-occurring vascular species, most of them representing hemicryptophyte and geophyte life forms. According to Ellenberg's values, the species pool indicated sunny (L7 and L8 species dominating) and semi-dry (mainly F4 species) habitat for the common ragweed population, whereas Zarzycki's soil granulometric data reflected coarse-grained soil. Albedo of the soil was lower than that of the vegetation thus sparsely vegetated sites were considered as heat accumulating microhabitats that might support establishment of the thermophilic A. artemisiifolia. During soil seed bank analysis a high number of naturally opened fruits (dehiscent achenes) were found (718.75 per m²) most probably indicating frequent germination in previous years. Greenhouse germination tests proved successful germination of 125 individuals per m², which was considered enough to maintain the A. artemisiifolia stand at the station. The results call attention to a newly discovered, established population with increasing demography of A. artemisiifolia, a highly allergenic introduced weed of Poland.
After the intramuscular administration of Engemycin L.A. inj. ad us. vet. (0.8 ml. 10kg⁻¹) to seven ewes for four consecutive days, the milk samples were collected at every 12 h of the experiment, from 0 to 166 h. The milk samples were analysed by the high performance liquid chromatography (HPLC) method. Twelve hours after the beginning of the experiment, all milk samples were positive for the presence of oxytetracycline (OTC) residues. The maximum mean concentrations were reached 12 h after the final administration (3.67 μg.g⁻¹). The OTC residues were not detected in any milk sample 84 h after the final administration.
Six cows suffering from mastitis were injected intramuscularly with tylosin at a dose of 10 mg kg⁻¹ b.w. for five consecutive days after the morning milking. The milk samples were collected for 13 days at 12 h intervals, and from the afternoon and morning milkings were pooled together (1:1). The samples were analysed by liquid chromatography and yoghurt inhibitor test for a detection of tylosin residues. Qualitative parameters of milk (active acidity, somatic cell count, total bacterial count) were also investigated. It was found that the HPLC method and yoghurt inhibitor test gave comparable results. The somatic cell count was above 400 000 per ml of milk. Staphylococcus aureus and pathogenic streptococci were detected in milk from mastitic cows.
The aim of the studies was the evaluation of relationship between the tetracycline residue levels in milk of cows treated intrauterinary with different doses of tetracycline and clinical form of endometritis. Six cows were treated by intrauterine route with pessaries containing tetracycline hydrochloride. The cows in group 1 (n = 3) were affected with acute endometritis, and the cows in group 2 (n = 3) by chronic endometritis. The animals were treated with Tetramykoin supp. uter. ad us. vet. (Léčiva, Praha, Czech Republic) at a dosage of 3g/cow (group 1), and 2g/cow (group 2). Milk samples were collected before treatment and at 12-h intervals for 144 h. The samples were analysed by the high-performance liquid chromatography method with solid phase extraction. The mean concentrations of tetracycline in milk 12 h after treatment were 227.7 μg.1⁻¹ in both groups. The tetracycline residues reached their maximum mean concentrations 24 h after the treatment (276.4 μg.1⁻¹, and 369.6 μg.1⁻¹, for group 1 and 2 resp.). Residues of 13.7 μg.1⁻¹ were detected in group 2 at 108 h. At 120 h the mean concentrations were 33.1μ g.1⁻¹ for group 1 and zero for group 2. No residues were detected after 144 h.
Blood serum concentrations and excretion of oxytetracycline (OTC) into milk after the i.m. administration of long-acting preparations in adult ewes were studied. Four ewes were treated with L.A. Engemycin and four - with L.A. Tetravet. OTC was administered to animals in a single dose of 20 mg/kg of live weight. OTC concentrations in blood serum were determined 1, 6 and 24 hours and then 2 to 8 days after the treatment. The milk samples were collected from the morning and evening milking up to 166 hours. OTC was determined by the HPLC method with solid phase extraction. Significantly higher serum concentrations of OTC (p < 0.05) were recorded after Tetravet administration from the 24th hour to the 5th day. OTC was detected in blood serum up to the 5th day after Engemycin administration, and up to the 6th day after Tetravet administration. The OTC residues in milk reached their maximum mean concentrations after 24 hours (2.525, and 2.186 mg/I, respectively). Then the mean OTC concentrations rapidly decreased and the residues were below the maximum residual limit (0.1 mg/l) 96 hours after the administration. OTC residues were detected in the first group up to 144 hours (0.021 mg/l), and up to 168 hours (0.031 mg/I) in the second group. The results indicated on the influence of the vehicle (polyvinylpyrrolidone in Engemycin and dimethylacetamide in Tetravet) on the serum kinetics and time of excretion of OTC into milk of treated ewes.
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