There is presented the information about history of the plant and production in ZPOW Hortex in Płońsk (actually member of Hortex Holding SA). There are described the works related to implementation of system HACCP (in the framework of PHARE project No PL 9607-01-12) on the lines of production of aseptic packed vegetable pastes and juices and nectars. Authoress presented the works from scope of "administration by quality" in former ZPOW Hortex Co. Ltd in Płońsk. She presented the implementation of HACCP system in present ZPOW Hortex in Płońsk. Authoress described the profits and experiences gained because of implementation of this system.
Mikrorozmnażanie podkładki Rosa canina 'Inermis' na podstawowej pożywce do rozmnażania róż nie przynosiło zadowalających efektów, zarówno ze względu na liczbę jak i jakość pędów. Osiągnięto znaczną poprawę efektywności namnażania pędów przez zastosowanie Gelritu i usuwanie wierzchołka wzrostu. Jakość mikrosadzonek była wysoka dzięki dodatkowi ryboflawiny do pożywki z auksyną lub przenoszenie sadzonek po zaindukowaniu korzeni do pożywki bez auksyny, z dodatkiem węgla aktywnego. Rośliny aklimatyzowały się w szklarni w około 90%.
Rose genotypes can be recalcitrant in in vitro regeneration. A lack of competence for regeneration results in unsatisfactory numbers of adventitious shoots, which diminishes achievements in use of genetic engineering in rose modern breeding. Among the factors which can partially overcome this problem is the use of thidiazuron (TDZ) in the induction phase of regeneration. We studied the use of TDZ in concentrations 0.05 and 1.0 mg l⁻¹, in the medium for the culture of donor shoots with the aim of increasing the regeneration competence of five rose cultivars differing in their regeneration ability. All the tested genotypes increased their regeneration potential when the donor shoots were cultured on TDZ media for 15 or 30 days. The strongest effect, doubling to quadrupling the number of shoots, was obtained in the cultivars characterized by the lowest regeneration potential. This enhanced regeneration ability was maintained through two or three subsequent 4-week long regeneration passages.
We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4℃ in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.