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The results of investigations concerning the advantages and disadvantages of implementing the accredited systems of management in testing laboratories were presented in the paper. The advantages and disadvantages were pointed out by staff of the laboratories. The discussion about results of implementing the accredited systems of management in testing laboratories functioning in Poland was undertook.
The aim of this paper was to study comparatively the amount, chemical composition and energy content of litter from forest and grassland ecosystems along a transect in Central Siberia, running from the north (68°N, Forest tundra) to the south (50°N, Dry steppes); it is about 2 000 km. In the boreal forests litter standing crop ranges from 15 to 78 t·ha⁻¹ d.w., in subboreal birch stands – it is about 17 t·ha⁻¹ d.w. and in grasslands – the litter standing crop decreases southward along the transect from 6 to 1.5 t·ha⁻¹ d.w. All kinds of litter are rich in reduced compounds and have negative oxidation degree. Nevertheless oxidation degree increased from North to South in both ecosystem groups: from –0.6 to –0.3 in the boreal forests and from –0.5 to –0.2 in the subboreal ecosystems. The chemical composition of litter is specific and differs from both initial substrates, i.e. phytomass and products of humification. The litter contains as many carbon as fulvic acids, as many oxygen as humic acids and much more hydrogen than both acids together. Energy content values of litter were estimated to be lower than that of humic acids and higher than energy contents of fulvic acids. The transformation of litter organic substances seems to be shifted to the formation of fulvic acids. The content of minerals in litter changed in both series (forest and grassland) in the following order Si> Ca> F> Al> S> Mg> K> P> Na.
Introduction. Schizophrenic patients present cognitive dysfunctions which are regarded to be one of endophenotypical markers predisposing to schizophrenia. Currently, schizophrenia can be treated as a neurodegenerative and neurodeveloping disease with genetic background. Objective. Assessment of the possible positive effect of neuropsychological rehabilitation in schizophrenia, in patients presenting cognitive dysfunctions. An additional aim was to verify the hypothesis that some genetic polymorphisms can be a prognostic factor for success in neuropsychological rehabilitation. Material and methods. 41 participants and 40 control subjects were randomly selected. Both groups had the diagnosis of paranoid schizophrenia. Cognitive functions were checked with the Wisconsin Card Sorting Test, Trail Making Test, and Stroop Test at the beginning and end of the experiment. In the research group, each patient trained with the rehabilitation programme RehaCom, whereas the control group did not receive such training. Genes COMT rs4680 and BDNF rs6265 were analysed in the genetic part of study. Results. RehaCom procedures appear to be useful in the neuropsychological rehabilitation of cognitive dysfunctions in patients diagnosed with schizophrenia. The research group showed a moderate improvement in the training programmes. Analysis of parameters obtained in the neuropsychological tests showed a slight improvement in both groups. At the present time, analysis of the polymorphisms of genes cannot be treated as a prognostic factor for the success of neuropsychological rehabilitation because statistical analyses showed few dependences with little statistical significance. Conclusions. Cognitive rehabilitation produces moderate improvement in cognitive functioning.
Some empirical evidence suggests that the endocannabinoids (eCB) (e.g. anandamide) may play an important role in cocaine addiction. The eCB act as a retrograde messengers activating CB receptors at the presynaptic membrane and are degraded by enzymatic actions of fatty acid amide hydrolase (FAAH). The present study aimed to examine the effect of the FAAH inhibitors, phenylmethylsulphonyl fluoride (PMSF; i.p.) or cyclohexylcarbamic acid 3-carbamoyl biphenyl-3-yl ester (URB597; i.p.) on the cocaine- or food-maintained self-administration as well as on the cocaine-seeking or food-taking behaviors in rats. Male Wistar rats were implanted with a catheter (iv.) and trained to self-administer cocaine (0.5 mg/kg/infusion) on a fixed ratio 5 schedule of reinforcement with a conditioned stimulus (tone+light). After self-administration stabilized, extinction/reinstatement procedures were carried out during which the rats were tested for the response reinstatement induced by cocaine (10 mg/kg, ip) or a cue (light+tone). The food (sweetened milk) self-administration and extinction/reinstatement procedures were conducted in a manner resembling cocaine self-administration. Neither PMSF (30–120 mg/kg) nor URB597 (0.1–3 mg/kg) affected cocaine self-administration. PMSF, 60 mg/kg, significantly reduced cocaine-induced reinstatement and at 120 mg/kg (combined with the challenge dose of cocaine) it evoked behavioral disruption. PMSF (60-120 mg/kg) dose-dependently inhibited cue-induced reinstatement. URB597 (1-3 mg/kg) attenuated both cocaine- and cue-induced drug-seeking behaviors. PMSF (60 mg/kg) decreased food self-administration. Toward reinstatement of food-taking behavior PMSF (60-120 mg/kg) and URB597 (3 mg/kg) showed inhibitory effects. Our results indicate that FAAH inhibitors could be potent modulators of motivational and conditioned aspects of goal-directed behaviors with less prominent effects on consumatory behaviors.
Gamma-butyric acid (GABA), a classical amino acid neurotransmitter, is implicated in the mediation of aggressive behaviour in both vertebrates and invertebrates including social insects. In social Hymenoptera queen absence usually has a suppressing effect on worker aggressiveness and may induce modifications of biogenic amine levels in worker brains. Effects of queen presence/ absence on worker brain levels of classical amino acid neurotransmitters were so far unexplored. To investigate that question and to elucidate the possible role of GABA in the mediation of ant aggressive behaviour we carried out HPLC measurements of GABA contents in individual brains of workers of two ant species, Formica polyctena (subfamily Formicinae) and Myrmica ruginodis (subfamily Myrmicinae) reared in queenright or queenless colony fragments. Immediately before killing the ants (only foragers) were tested in dyadic aggression tests consisting of an encounter with a nestmate, an alien conspecific, or a small larva of the house cricket (Acheta domesticus). In spite of significantly smaller brain weight of workers of M. ruginodis, worker brain GABA levels were significantly higher in M. ruginodis than in F. polyctena. Queen absence was associated with significantly increased brain GABA levels in F. polyctena, but not in M. ruginodis. Brain GABA levels of the tested ants did not depend on the type of the aggression test, and no significant interaction was discovered between the aggression test type and queen presence/ absence. We demonstrate for the first time that queen presence/ absence may influence not only biogenic amine levels, but also levels of classical amino acid neurotransmitters in ant worker brains. Our data also imply that not only brain levels of classical neurotransmitters, but also modifications of these levels induced by changes of social context (queen removal) may significantly differ between various ant phyla.
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