INTRODUCTION: tDCS mapping of zones of brain with antiseizure potency might reveal their role in kindled seizures development. AIM(S): To investigate the effects of cathode tDCS performed at different zones of brain upon generalized seizures induced in pentylenetetrazol (PTZ) – kindled rats. METHOD(S): Kindling model of chronic generalized seizures were induced in rats via daily i.p. administration of PTZ (30 mg/kg) in male Wistar rats during three weeks. Only animals with generalized clonic – tonic fits as response to last three administrations of PTZ were used for further observation. tDCS were performed with cathode electrode with diameter of 7.5 mm which was located on the left side to bregma as a first type of position and at middle line to lambda as a second position. It was assumed that the first electrode location permitted to affect the frontal parts of cortex while the second one permitted to affect cerebellar cortex. The intensity of applied current was 0.7 mA, and tDCS was performed during 15 min. In 10 min from the moment of cessation of tDCS the PTZ was i.p. injected with a dosage of 30.0 mg/kg. Control group of PTZ-kindled animals was false stimulated. RESULTS: Latency of seizures induced after tDCS of frontal cortical zone was not differfrom such one in the control group. The generalized fits were protected in 5 out of 10 animals, while in the control group generalized fits were observed in all 10 animals (P<0.05). After tDCS at posterior zone latency of seizures exceeded such one in the control group by 35.0% (P<0,05) and generalized fits were not observed (10 rats), which was also significantly differ from group with tDCS at hemisphere. Besides, cerebellar stimulation prevented manifestations of postseizure depression. CONCLUSIONS: Cathode tDCS applied to posterior zone of skull induced more pronounced antiseizure effects upon PTZ-kindled seizures when compared with tDCS of hemisphere. Such an effect might be explained by the involvement of cerebellar structures in the mechanisms of seizures suppression.
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