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Prevalence of mecA, blaZ, tetO/K/M, ermA/B/C, aph, and vanA/B/C/D genes conferring resistance to oxacillin, penicillin, tetracycline, erythromycin, gentamicin, and vancomycin was investigated in 65 staphylococcal isolates belonging to twelve species obtained from ready-to-eat porcine, bovine, and chicken products. All coagulase negative staphylococci (CNS) and S. aureus isolates harbored at least one antibiotic resistance gene. None of the S. aureus possessed more than three genes, while 25% of the CNS isolates harbored at least four genes encoding resistance to clinically used antibiotics. In 15 CNS isolates the mecA gene was detected, while all S. aureus isolates were mecA-negative. We demonstrate that in ready-to-eat food the frequency of CNS harboring multiple antibiotic resistance genes is higher than that of multiple resistant S. aureus, meaning that food can be considered a reservoir of bacteria containing genes potentially contributing to the evolution of antibiotic resistance in staphylococci.
Listeria monocytogenes, a significant food-borne pathogen, must defy a variety of conditions encountered in the food environment and during the infection process. In reaction to adverse conditions, the bacteria significantly change their metabolism, inducing a stress response which is mediated by a range of alternative sigma factors. The extent of the response to stress was shown to vary in the L. monocytogenes population. According to recent evidence a major L. monocytogenes alternative sigma factor, designated sigma B (σB), regulates some virulence genes in response to stress, which supports an older hypothesis that stress-resistant strains should be more pathogenic. The induction of σB-dependent genes may also be important from the point of view of food hygiene. It seems that stress response activation can paradoxically enhance resistance to agents used in food preservation. Therefore, monitoring the expression of σB-dependent genes can serve as a useful marker to assess the innate resistance of L. monocytogenes strains. This knowledge will allow the design of new methods with sequential preservation steps that could inactivate the bacteria without inducing their stress response.
Real-time PCR directed to intergenic spacer (IGS) noncoding region between 16S and 23S rRNA genes was used for species specific detection of Mycoplasma felis in conjunctival scrapings. Samples were collected from 57 cats suffering from chronic conjunctivitis in 2008-2010 (Wrocław, Poland). Samples from 36 cats (63.2%) were shown to be positive for Mycoplasma felis. Our research gives a first insight in the occurrence of Mycoplasma felis among domestic cats in Poland suggesting that this pathogen may constitute an underestimated cause of chronic conjunctivitis.
Staphylococcal food poisoning results from ingestion of food contaminated with toxins produced by enterotoxigenic Staphylococcus aureus strains. Common symptoms of this intoxication include vomiting, diarrhea, and abdominal cramps. Staphylococcal enterotoxins are resistant to heat and a number of environmental factors. Certain cheeses, milk powder, and whey powder are the only foodstuffs that are being routinely examined for the presence of staphylococcal enterotoxins SEA-SEE. The newly identified enterotoxins are not included in the current examination scheme. Enterotoxin-producing staphylococci were already isolated from meat, meat products, milk, dairy products, fermented food products, vegetables, pastries and fish products. It has been demonstrated that many environmental factors associated with food processing and storage can significantly influence the level of secreted enterotoxins by S. aureus strains. Nevertheless, only a few studies on the production of staphylococcal enterotoxins were conducted in foodstuffs. Most data on their expression is based on experiments performed with a low number of S. aureus strains, and usually only SEA-SEE enterotoxins are investigated. These results inclined many authors to the conclusion that milk and dairy products are unfavorable environments for expression of staphylococcal enterotoxins. However, recent research has indicated a significant heterogeneity in the ability of enterotoxin production in milk among S. aureus strains derived from diverse sources. S. aureus strains able to secrete high levels of enterotoxins in milk and meat juice were described. This research indicates that a high number of S. aureus strains should be used for studying staphylococcal enterotoxins expression in food. It seems to be the appropriate way to assess the risk of staphylococcal food poisoning.
The genus Arcobacter was included in the family Campylobacteraceae in 1991, and currently consists of fourteen species, of which A. butzlerii, A. cryaerophilus, and A. skirrowii have been associated with human and animal diseases. Arcobacter spp. have been isolated from food, mainly from products of animal origin, with the highest prevalence in chickens, followed by pork and beef. The bacteria have been commonly detected in chicken carcasses and in the intestinal content of birds. The faecal contamination is regarded as the main route of transmission of Arcobacter spp. into poultry carcasses. Additionally, these bacteria can attach to water pipes and are able to survive in the slaughterhouse environment under chilled conditions. Apart from poultry and other meat, Arcobacter spp. have been isolated from drinking water reservoirs, sewage, faeces of healthy animals and from animals affected by various diseases, including abortion, mastitis, septicaemia, and enteritis. Recent evidence suggests that Arcobacter spp., especially A. butzleri, may be involved in human enteric diseases. Occasionally, these bacteria have also been found in cases of human extraintestinal diseases. Until present, little is known about the infection dose, mechanisms of pathogenicity, and virulence factors of Arcobacter spp. Consumption of raw or poorly cooked contaminated food of animal origin, mainly poultry, is the most likely source of human poisoning. The actual role of Arcobacter spp. in human diseases is probably underestimated because of the lack of standardized identification methods and routine detection procedures.
Entomopathogenic fungi attack the host insect and therefore may be potentially used for insect control. Penetration of cuticle is achieved by a combination of mechanical pressure and enzymatic degradation. The entomopathogenic fungi produce a variety of exocellular hydrolytic enzymes: proteases, chitinases and lipases, which are capable to digest the major components of insect cuticle. This review describes the characteristics of these enzymes and their role in pathogenesis.
Nitric oxide (NO, earlier known as EDFR - Endothelium-Derived Relaxing Factor) is a multifunctional particle involved in physiological as well as pathological reactions. The specificity of these reactions depends on the NO concentration, location and interactions with other particles. Nitric oxide affects the dilatation of blood vessels and immunological reaction. It influences cardiovascular myocyte tonus (contraction) and counteracts vasoconstrictive factors such as endothelin-1 and angiotensin-II, thereby ensuring proper tissue perfusion depending on current requirements. NO protects vessel walls by preventing lipid peroxidation and decreasing the activity of reactive oxygen radicals. Nitric oxide shows anticoagulant properties by inhibiting the adhesion, activation and aggregation of trombocytes.
Staphylococcal enterotoxins (SEs) are emetic toxins causing food poisoning in humans, because of their biological activity and structural relatedness They have been classified as members of the pyrogenic exotoxin superantigen family Among them nine major antigenic types of emetic enterotoxins were recognized In recent years several newly detected SEs were also discriminated, but their occurrence and role in human and animal diseases are not fully understood Neverthless, evidences of their pathogenic role and broad distribution in staphylococcal strains cumulate Therefore their importance as potential risk factor for food safety becomes essential For this reason their properties, genetic determinants and supposed mechanisms of the pathogenic activity are discussed in respect of their potential hazard to human health.
A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows’ milk in the milk of goats. The PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and cows’ mitochondrial DNA fragment prevented false negative results. The method was applied to track the presence of cow DNA in goat milk available on the Polish market. A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined. In 33 samples, cow DNA was detected, while 21 samples, including all of the 20 samples from foreign producers, produced the goat-specific product only.
Aim of the study: The study was conducted to determine the incidence of genes encoding emetic staphylococcal enterotoxins (SEs) in S. aureus isolates from pork and pigs, and to demonstrate the connection between the enterotoxigenic potential of S. aureus and its genetic background. Materials and methods: S. aureus isolates from pork (45 isolates) and pigs (45 isolates), representing various clonal complexes, were tested for the presence of emetic SEs genes. Results and discussion: Thirty-four of the 45 S. aureus isolates (75%) derived from pork were shown to harbor genes encoding emetic SEs. Among 45 pig-derived S. aureus isolates, SE genes were detected in 28 isolates (62%). Fifty-five percent of potentially enterotoxigenic staphylococci carried genes encoding classical toxins (SEA-SEE), whereas 28 isolates (45%) harbored exclusively genes encoding new emetic SEs. The most prevalent (82%) classical enterotoxin gene was seb, whereas seg and sei genes dominated (82%) among isolates harboring genes encoding other emetic toxins. Seventeen of 23 S. aureus isolates assigned to the CC15 clonal complex were found to harbor the seb gene. Ten of 15 CC7 isolates contained the selp gene. Isolates harboring seg and sei genes dominated in CC30 (81%) and CC9 clones (76%). Four isolates assigned to CC398 were shown to harbor enterotoxin genes, such as seb, sed, seg, sei, and ser. Our results indicate a high incidence of enterotoxigenic S. aureus isolates harboring genes encoding other emetic SEs in pork and pigs. In most of the pig- and pork-derived isolates studied here, genotype-enterotoxin association was similar to that known from human S. aureus isolates. This is the first report on SE genes in S. aureus CC398 genetic background in Poland, and probably also in Europe.
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