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Background. The Siberian sturgeon, Acipenser baeri, is one of the most important sturgeon species cultured in Poland. The effective management of aquaculture production of this species requires contemporary knowledge of broodstock structure, mating patterns, and genetic diversity of broodstock. The aim of the present study was the application of microsatellite DNA analysis for estimation of gene diversity in the Siberian sturgeon farmed at a Polish fish farm. Materials and Methods. Fin clips were randomly sampled from 94 specimens of Siberian sturgeon broodstock reared at the Wąsosze Fish Farm near Konin, Poland. The analysed broodstock has been kept there since 1996, with new specimens being introduced annually. The fish were studied in 2007–2008. Genomic DNA for amplification of microsatellite loci was extracted using Chelex 100. Six microsatellite loci (Afu-19, Afu-39, Afu-68, AfuB-68, Spl-163, and Spl-168) were amplified for examination of the genetic variability of the studied fish. Results. Within 94 individuals of the Siberian sturgeon, a total of 74 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 8 to 18, with an average allele number being 12. The genetic diversity of six microsatellite loci varied from 0.686 to 0.811. Conclusion. This technology has great potential for use in aquaculture of sturgeon fish, especially when levels of genetic variation could be monitored and inbreeding controlled in commercial breeding programs.
DNA finferprinting analysis based on microsatellites was applied for separation of mixed gynogenetic offspring of Siberian sturgeon (Acipenser baeri) and individuals from commercial production. Variation at 11 microsatellite DNA loci was surveyed for parent of gynogenetic offspring. Thus microsatellite DNA profiles in studied loci were known and this key-point was aplied in segregation analysis of mixed fish. In results 108 individuals of 281 studied were verified as gynogenetic offspring. The present survey of microsatellite variation demonstrated a reliable tool for separation of mixed group of fish.
Six microsatellite loci were used to examine the genetic variability of Russian sturgeon and sterlet cultured in a Polish fish farm. Within 47 individuals of Russian sturgeon and 35 individuals of sterlet, a total of 113 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 9 to 23 in Russian sturgeon and 3–8 in sterlet. The genetic diversity of six microsatellite loci varied from 0.404 to 0.880 in Russian sturgeon and from 0.515 to 0.971 in sterlet. Microsatellite analysis has a great potential for aquaculture of sturgeon fishes, especially when levels of genetic variation could be monitored and inbreeding controlled in commercial breeding programs.
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The genetic characteristic of seriously threatened river lamprey Lampetra fluviatilis L. from Vistula river and Vistula lagoon (Poland) was described in the present paper. The study, based on the nine microsatellite markers, was conducted to determine the genetic diversity and population structure of river lamprey in Vistula river and Vistula lagoon in Poland. Observed heterozygosity ranged from 0.158 to 0.974 in lamprey from Vistula river, and 0.040 to 0.990 in lamprey from Vistula lagoon. The expected heterozygosity in river lamprey ranged 0.177–0.673 in specimens and 0.213–0.670 in population from Vistula lagoon. Eight loci appear to be diagnostic, due to occurrence of private alleles, for distinguishing the Vistula river and Vistula lagoon populations. The estimated effective population size (Ne) for the studied populations of river lamprey form Vistula river and Vistula lagoon equalled 72.4 (95% CI = 14.9–∞) and 25.8 (95% CI = 8.7–151.1), respectively. Constructed individual’s tree based on DAS genetic distances and the Principal Coordinates Analysis (PCoA) exhibited three main genetic groupings within studied fish group. The presented genetic characteristic of studied lamprey populations is important and necessary to develop and implement conservation actions of river lamprey in Poland
The colour of light is a very important environmental factor that affects fish physiology. The aim of the present study was to evaluate the effects of different colours of light on weight gain, length, condition factor and specific growth rates of the juvenile rainbow trout Oncorhynchus mykiss. The study employed fertilized eggs that were exposed separately to seven colour lamps including white (573 nm, control), azure (397 nm), blue (459 nm), green (524 nm), yellow (586 nm), orange (611 nm) and red (742 nm). The experiment was conducted for 5 months (from fertilization until the fish reached 2 g wet weight) at the Sheshpir fish farm (west of Fars province), Iran. After yolk sac absorption, the weight and length of randomly sampled fish were measured monthly. The results showed that weight parameters of fish exposed to yellow (0.562 ± 0.13) and white (0.547 ± 0.13) coloured light were higher (p ≤ 0.05) than fish subjected to the other colours. The highest length growth was observed in fish exposed to yellow (3.91 ± 0.16) and white (3.61 ± 0.10) light, respectively. The highest growth rate (4.641 ± 0.29) and condition factor (2.00 ± 0.03) were observed in fish maintained under yellow light.
The aim of the present study was to assess the genetic variability of whitefish (Coregonus lavaretus L.) from Lake Vistytis using microsatellite DNA markers. The genetic analysis indicated a high level of polymorphism at the investigated microsatellite loci with 78 alleles identified among four microsatellite markers. The average values of the He, Ho, and PIC parameters were 0.92, 0.85, and 0.89, respectively, and were high for all the studied loci. The present genetic analysis is the first molecular study of the whitefish population from Lake Vistytis.
Experimental rearing of sterlet using forms with normal and albino coloring was conducted. The spawn was fertilized as follows: spawn from females with normal coloring were fertilized with milt obtained from males with normal coloring (group CC) and with that from albino males (group CB); spawn from albino females were fertilized with milt obtained from males with normal coloring (group BC) and with milt obtained from albino males (group BB). It was confirmed that the hatch inherited parental coloring; there were only individuals with dark coloring in group CC, while in group BB, there were only albino individuals. However, in groups BC and CB, in addition to the majority of individuals that inherited color, there were individuals of median coloring. The highest hatch survival rate was noted in group CC at 97.3%, and the lowest was noted in group CB at 94.8%. The hatch with albino coloring had the slowest growth rate.
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