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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Starzenie a długowieczność

100%
Gregoraszczuk E. L.
Wszechświat
|
2017
|
tom 118
|
nr 10-12
2

Wplyw ksenoestrogenow i innych zanieczyszczen srodowiska na zdrowie

100%
Gregoraszczuk E. L.
Wszechświat
|
2008
|
tom 109
|
nr 10-12
263-267
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Hormonalnie czynne związki w środowisku a epidemia otyłości

100%
Gregoraszczuk E. L.
Wszechświat
|
2021
|
tom 122
|
nr 04-06
4

Ability of isolated ovarian cell types to form aggregates in vitro

100%
Gregoraszczuk E. L.
Folia Histochemica et Cytobiologica
|
1990
|
tom 28
|
nr 3
5

Is progesterone a modulator of luteal steroidogenesis in pig? A tissue culture approach

100%
Gregoraszczuk E. L.
Folia Histochemica et Cytobiologica
|
1994
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tom 32
|
nr 1
6

The effect of triiodotyronine [T3] and TSH treatment in vitro on progesterone production by luteal cells isolated during different stages of the luteal phase

100%
Gregoraszczuk E. L.
Journal of Physiology and Pharmacology. Supplement
|
1996
|
tom 47
|
nr 1
7
Content available remote

Superactive human leptin antagonist reverses leptin-induced excessive progesterone and testosterone secretion in porcine ovarian follicles by blocking leptin receptors

63%
Gregoraszczuk E. L., Rak A.
Journal of Physiology and Pharmacology
|
2015
|
tom 66
|
nr 1
8

Effect of ghrelin on proliferation, apoptosis and secretion of progesterone and hCG in the placental JEG-3 cell line

63%
Rak-Mardyla A., Gregoraszczuk E. L.
Reproductive Biology
|
2010
|
tom 10
|
nr 2
9

Wplyw hormonu wzrostu na steroidogeneze komorek jajnikowych

63%
Gregoraszczuk E. L., Gertler A.
Postępy Biologii Komórki. Suplement
|
1999
|
tom 12
207-212
10
Content available remote

ERK 1/2 and PI-3 kinase pathways as a potential mechanism of ghrelin action on cell proliferation and apoptosis in the porcine ovarian follicular cells

63%
Rak-Mardyla A., Gregoraszczuk E. L.
Journal of Physiology and Pharmacology
|
2010
|
tom 61
|
nr 4
451-458
Recently, we reported the stimulatory effect of ghrelin on ovarian cell proliferation in parallel with the inhibitory action of ghrelin on cell apoptosis. The aim of the presented data propose local activation of extracellular signal-regulated protein kinase 1 and 2 (ERK 1/2) and phosphoinositide-3 (PI-3) kinase pathways as a mechanism of ghrelin effect in the porcine ovary. To test this hypothesis, action of ghrelin on levels of ERK 1/2 with PI-3 kinase activity and protein expression using ELISA and western blot analysis, respectively, was examined. Additionally, to determine which pathways (ERK 1/2 or PI-3 kinase) are the potential signals of ghrelin-mediated cell proliferation and apoptosis in ovarian cells, we used PD098059 (50 µM) and wortmannin (200 µM), well-known inhibitors of these kinases. Treatment of ovarian coculture cells with ghrelin (100, 250, 500 and 1000 pg/ml) showed stimulation of phospho-ERK 1/2 levels and PI-3 kinase activity, with the maximum effect observed after 15 min of cell incubation. Additionally, western blot analysis indicated that ghrelin increased expression of both kinases. Moreover, ghrelin used in combination with PD098059 or wortmannin significantly decreased cell proliferation, which was measured by the Alamar Blue assay and increased apoptosis, which was measured by caspase - 3 activity and DNA fragmentation. In conclusion, these results suggest that the ERK 1/2 and PI-3 kinase pathways may be potential signals of ghrelin mediate the cell proliferation and apoptosis of ovary cells.
11
Content available remote

Expression of ghrelin and its receptor in porcine ovarian follicles collected from prepubertal and estrous cycle animals

63%
Rak-Mardyla A., Gregoraszczuk E. L.
Journal of Physiology and Pharmacology
|
2012
|
tom 63
|
nr 2
12
Content available remote

Modulatory effect of ghrelin in prepubertal porcine ovarian follicles

63%
Rak A., Gregoraszczuk E. L.
Journal of Physiology and Pharmacology
|
2008
|
tom 59
|
nr 4
781-793
Ghrelin is a novel growth hormone-releasing peptide, originally identified in rat stomach as an endogenous ligand of the growth hormone secretagogue receptor. Ghrelin is an important regulator of growth hormone secretion, food intake, and reproductive function. This study investigates whether or not ghrelin can modulate prepubertal pig ovary function, which was measured as ovarian estradiol secretion, aromatase activity, cell proliferation, and apoptosis. To investigate this, ovarian cells were co-cultured with four different doses of ghrelin (100, 250, 500, and 1000 pg/ml) for 48 h. Culture media samples were collected, and estradiol levels were determined, while aromatase expression was measured in the cultured cells. Cell apoptosis was measured by determination of caspase-3 activity, DNA fragmentation and TUNEL assay. Ghrelin in 250 and 500 pg/ml doses stimulated estradiol secretion. At all doses ghrelin stimulated aromatase activity and protein expression. Moreover, ghrelin increased cell proliferation and decreased apoptosis. This study provides novel evidence that ghrelin has a modulatory effect in the ovary. We suggest two mechanisms that explain how ghrelin acts on estradiol secretion: 1) ghrelin directly influences aromatase activity and protein expression; 2) ghrelin stimulates cell proliferation and antiapoptotic actions.
13

Lysosomal acid phosphatase activity and progesterone secretion by porcine corpora lutea at various periods of the luteal phase

63%
Gregoraszczuk E. L., Sadowska J.
Folia Histochemica et Cytobiologica
|
1997
|
tom 35
|
nr 1
14

The cytoskeleton proteins and LH-regulated steroidogenesis of porcine luteal cells

63%
Gregoraszczuk E. L., Slomczynska M.
Folia Histochemica et Cytobiologica
|
1996
|
tom 34
|
nr 1
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Thyroid hormone action in porcine luteal cells. Effect of triiodothyronine on mitochondrial cytochrome P450-SCC activity

63%
Gregoraszczuk E. L., Pieklo R.
Journal of Physiology and Pharmacology
|
1998
|
tom 49
|
nr 3
16

The cytoskeleton proteins and Prl-regulated steroidogenesis of porcine theca cells

63%
Gregoraszczuk E. L., Stoklosowa S.
Folia Histochemica et Cytobiologica. Supplement
|
1997
|
tom 35
|
nr 2
17

Triiodotyronine [T3] modulates cAMP accumulation in cultured porcine theca, granulosa and luteal cells

63%
Gregoraszczuk E. L., Galas J.
Folia Histochemica et Cytobiologica. Supplement
|
1997
|
tom 35
|
nr 2
18
Content available remote

A low halogenated biphenyl (PCB3) increases CYP1A1 expression and activity via the estrogen receptor beta in the porcine ovary

51%
Ptak A., Ludewig G., Gregoraszczuk E. L.
Journal of Physiology and Pharmacology
|
2008
|
tom 59
|
nr 3
577-588
Polychlorinated biphenyls (PCBs) have been detected at high levels, up to hundreds of pg/ml, in human ovarian follicle fluid. The effect of PCBs on the ovary and the consequences of exposure are largely unknown. We have previously shown that PCB3 (4-chlorobiphenyl) increases the secretion of estradiol and the activity of cytochrome P450s (CYPs) in ovarian follicle cells. Our goal here is to elucidate the mechanism of CYP induction by this congener. Exposure of porcine follicle cells, a co-culture of theca and granulosa cells, to 6 ng/ml of PCB3 caused an increase in CYP1A1 protein and enzymatic activity, in the same manner as exposure to exogenous 17ß-estradiol. No changes were seen in the protein level of the aryl hydrocarbon receptor (AhR), which mediates the first step in the signaling pathway of CYP1A1 induction. However, a strong reduction was seen in the protein level of estrogen receptor beta (ERß), while no effect was seen on ER protein levels. These result suggest that: 1) PCB3 acts as an agonist of ERß but not the Ah receptor in the ovarian follicles, 2) PCB3 is not only an efficacious inducer of CYP1A1 expression and activity, but also a substrate for this enzyme. Changes in the expression level of CYP1A1 not only alter the intensity of the activity of PCB3, but also the activity of estrogen in the ovary.
19

The effect of prostaglasndin F2alpha and its analogues on progesterone, estradiol and adrogen production by the cultured bovine luteal cells

51%
Gregoraszczuk E. L., Wojtusiak A., Rozanska A.
Folia Histochemica et Cytobiologica
|
1988
|
tom 26
|
nr 4
20

Different action of ovine GH on porcine theca and granulosa cells proliferation and insulin-like growth factors I - and II-stimulated estradiol production

51%
Kolodziejczyk J., Gregoraszczuk E. L., Leibovich H., Gertler A.
Reproductive Biology
|
2001
|
tom 01
|
nr 1
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