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The aim of this study was to determine the effect of various dietary supplements on the level of selected unsaturated fatty acids, with potential antibacterial properties, in the hepatopancreas of Cepaea nemoralis snails, and to perform a preliminary phenotypic characterization of Citrobacter braakii strains occurring in the intestinal tract of these snails, which are environmental reservoirs for these bacteria. Gas chromatography was used for fatty acid analysis of the snails. Biochemical characteristics of Citrobacter braakii isolates and their resistance profiles for six chemiotherapeutics were determined. Giving Cepaea nemoralis food supplemented with Allium sativum (garlic), Urtica dioica (stinging nettle) and Aloe vera caused an increase in the total unsaturated fatty acids in the hepatopancreas. In the invertebrates studied, the Aloe vera and Allium sativum supplements caused an increase in arachidonic acid, while Utrica dioica increased oleic and linoleic acid content, which probably contributed to the 2-log CFU (colony forming unit) reduction of Citrobacter in the intestine. Wild snails of the genus Cepaea should be taken into consideration in epidemiological investigations as potential vectors for pathogenic strains of Citrobacter spp.
The purpose of the study was both to estimate the electrophoretic profiles (SDS-PAGE) of outer membrane proteins among Salmonella Enteritidis strains isolated from humans and poultry, as well as to identify the antigens which can induce the appearance of a specific antibody response among vaccinated animals. The densitometry analysis of electrophoregrams showed that a single protein complex of 21 fractions was distinctive for strains grown in standard conditions. Among these fractions those with 20, 22, 35 and 37 kDa characterized by high optical density were found dominant. A single protein profile with 24 fractions was also characteristic of strains grown in conditions with restricted access to iron ions. A high optical density was a feature of proteins with 19, 35, 37, 55 and 74 kDa. Adding of 200 µM 2,2’-dipirydyl to the culture medium caused the expression of proteins and 78 kDa taking part in obtaining and transporting iron through S. Enteritidis within a mass range of 19, 55, 70, and 74. Densitometry analysis of electrophoregrams obtained from nitrocellulose membranes for proteins from bacterial strains grown in conditions with restricted access to iron ions indicated the presence of specific, intensive reactions towards the antigens of 35 and 55 kDa. As far as proteins obtained from bacterial culture grown with and without the addition of chelator were concerned, distinctive reactions were found towards antigens of 35 and 58 kDa. The main protein present in electrophoregrams of bacteria grown both with and without a supplement of chelator and in immunoblotting reactions was that of 35 kDa, belonging to porins of OmpA type.
Forensic entomology frequently assists forensic medicine in legal investigations. It makes it possible to estimate the time of death when a cadaver is recovered at a relatively advanced stage of decomposition. In criminalistics practice, unburied bodies are found the most commonly, and therefore the fauna of these cadavers is the best investigated. The aim of this study was to collect a succession of insects and other invertebrates occurring on an unburied corpse. The experiment was conducted on the carcass of a cat euthanized due to an advanced cancer process. The carcass was colonized by three phyla of animals: Annelidae, Mollusca, and Arthropoda. They belonged to 7 classes and 10 orders. The most diverse were Arthropoda. They were classified into 5 classes: Insecta, Diplopoda, Malacostraca, Entognata, and Arachnida, and into 8 orders: Julida, Isopoda, Collembola, Diptera, Coleoptera, Hemiptera, Araneae, and Acari. The fly species Calliphora vicina from the family Calliphoridae is of particular interest among the insects collected because it is one of the fundamental indicator species whose life cycle makes it possible to determine an approximate time of death. During the study it was noted that arthropods occurred in a certain pattern of succession, predictable in forensic entomology. The first group was Calliphora vicina (Calliphoridae, Diptera), which laid eggs. The next (second) group consisted of first-instar C. vicina larvae and insects feeding on these larvae, such as Philonthus tenuicornis (Staphylinidae, Coleoptera). The first stage of succession was the appearance of eggs of C. vicina. The second phase was the appearance of adult flies other than Calliphoridae and of accidental species, as well as beetles (e.g. Philonthus tenuicornis, Staphylinidae, Coleoptera) feeding on larvae of C. vicina. The third phase of succession was the appearance of all larvae stages of C. vicina that continued and finished their life cycle.
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