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1
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Phytochemical variability of coltsfoot (Tussilago farfara L.) in Poland

100%
Adamczak A., Buchwald W., Gryszczynska A.
Herba Polonica
|
2012
|
tom 58
|
nr 4
Coltsfoot leaves are a traditional raw material, rich in polysaccharides and phenolics. The variability of the contents of these main compounds was determined, using plant material originated from 22 natural populations of Tussilago farfara L. growing in various regions of Poland. In the years 2008–2009, plants from each investigated population were collected in the Garden of Medicinal Plants in Plewiska near Poznań (Poland). Coltsfoot leaves were harvested in the middle of June and July of 2010, and then dried at room temperature. In these raw material we quantified swelling index (describing mucilage content) and spectrophotometrically: the amounts of total polyphenols, polyphenols unadsorbed on hide powder (non-tannin phenolics) and tannins (expressed as pyrogallol equivalent) as well as the sum of hydroxycinnamic acid derivatives (expressed as rosmarinic acid) and flavonoids (expressed as quercetin). The demonstrated results show the relatively high and balanced contents of the basic active compounds, especially flavonoids (0.7–1.3%) and polysaccharides (swelling index: 8.0–14.5). In addition, it was found that flavonoids and mucilage in coltsfoot leaves fluctuate in only a small range (V=11–13%), regardless of overshadow and the harvest time of raw material.
2
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Content of pyrrolizidine alkaloids in the leaves of coltsfoot (Tussilago farfara L.) in Poland

100%
Adamczak A., Opala B., Gryszczynska A., Buchwald W.
Acta Societatis Botanicorum Poloniae
|
2013
|
tom 82
|
nr 4
Coltsfoot (Tussilago farfara L.) is a common species, widely used in European and Chinese traditional medicine for the treatment of respiratory diseases. However, raw material from this plant contains hepatotoxic pyrrolizidine alkaloids (PAs). The aim of the study was to determine the variability of the level of PAs (senkirkine and senecionine) in leaves of coltsfoot originated from natural populations in Poland. In the phytochemical analysis, 20 samples of T. farfara were used. This plant material was obtained from the Garden of Medicinal Plants in Plewiska near Poznań and originated from different regions of Poland. Coltsfoot leaves were harvested in the middle of July of 2010 and then dried at room temperature. The alkaloid content was detected using the HPLC-DAD method. The amount of PAs in leaves of T. farfara changed in a wide range from 0.06 to 1.04 μg g−1 of dry matter (DM). The content of senkirkine and senecionine was positively correlated (r = 0.68, P = 0.001). There was no statistically significant correlation between the amount of PAs as well as leaf weight and water content in leaves of T. farfara. Our results showed that a medium-sized leaf of coltsfoot (0.33 g DM) may contain from 0.02 to 0.34 μg of PAs (on average 0.14 μg). The level of PAs was not associated with the region of Poland, but phytochemical similarity of samples was usually visible at the local scale. Coltsfoot leaves are characterized by a high variability of the content of toxic PAs, much higher than in the case of the main active compounds, especially flavonoids and mucilage. This phytochemical variability is mainly genetically determined (samples came from a garden collection), and it can be increased by environmental factors. Our investigations indicate that Polish natural populations of T. farfara may provide raw material with a low level of toxic PAs.
3
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Biometric and phytochemical variability of roseroot (Rhodiola rosea L.) from field cultivation

100%
Adamczak A., Gryszczynska A., Buchwald W.
Herba Polonica
|
2014
|
tom 60
|
nr 1
Roseroot (Rhodiola rosea L.) is an adaptogenic plant, widely used in the traditional medicine of Scandinavia, Russia, China and Mongolia. The aim of the study was to determine the biometric and phytochemical co-variability of this species under field cultivation in western Poland (Plewiska near Poznań). The plant material originated from four-year-old cultivation established twice by rhizome division in autumn 2007 and 2008. In the study, 46 individuals (23 plants in 2011 and in 2012) were used. The biometric analysis included measurements of the basic characteristics of plants related to the size (luxuriance) of clumps, shoots, leaves and rhizomes with roots (raw material). The amounts of total polyphenols (expressed as gallic acid), tannins (expressed as pyrogallol) and flavonoids (expressed as quercetin) were determined spectrophotometrically. The obtained results showed high variation of Rh. rosea, especially in the level of flavonoids (0.01–0.20% DM) and in the weight of raw material (113–1156 g FM/plant). There were observed correlations between the phytochemical (flavonoid and total phenolic content) and biometric (water content, leaf number, shoot and clump size) features.
4
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Biometric features and content of phenolic compounds of roseroot (Rhodiola rosea L.)

100%
Adamczak A., Buchwald W., Gryszczynska A.
Acta Societatis Botanicorum Poloniae
|
2016
|
tom 85
|
nr 3
Roseroot (Rhodiola rosea L.) belongs to important herbs in folk medicine of Scandinavia, Russia, Mongolia, and China. Its therapeutic usage is mainly associated with the adaptogenic properties of this species. Roseroot is characterized by high morphological, phytochemical, and genetic differentiation. The aim of the present work was to determine the biometric and phytochemical co-variability of this taxon. Samples of Rh. rosea were collected from 4-year-old experimental field cultivation established by rhizome division in western Poland. For each plant, the biometric measurements of the clumps, shoots, leaves, and rhizomes with roots were carried out. In the underground plant parts (raw material), the contents of the main active compounds (phenylpropanoids, phenylethanoids, phenolic acids, and catechins) were determined by the HPLC-DAD method. K-means clustering analysis showed three well-separated plant groups of Rh. rosea that differed significantly in the level of most of the investigated components. It was interesting that in the raw material with a high content of phenylethanoids, a low level of phenyl­propanoids was found, and vice versa. These chemical groups clearly differed in luxuriance of plants, too. The important diagnostic feature was also the degree of leaf serration. The morphological and phytochemical co-variability of roseroot was confirmed by the correlations detected between some active compounds (especially catechins and rosavin) and biometric traits describing the size and serration of leaves, the size of clumps and shoots as well as the weight of the raw material.
5
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The determination of flavan-3-ol content in the root of Rhodiola kirilowii

100%
Gryszczynska A., Mielcarek S., Buchwald W.
Herba Polonica
|
2011
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tom 57
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nr 1
According to a new classification, nearly 90 species belong to the Rhodiola family. However, only three of them are most frequently used in alternative medicine. In addition to R. rosea and R. quadrifida, R. Kirilowii can also be included in this group. This species was investigated for flavan-3-ol content. Due to beneficial properties of this class of compounds (free radical scavenging and reactive oxygen forms), the researchers decided to examine 5 substances in the root and in two extracts, alcoholic and aqueous ones. Depending on the matrix analysed, the content of particular components varies. Furthermore, it is also affected by extraction time as well as a particular extraction solvent employed. An ultra performance liquid chromatograph connected to a tandem mass spectrometer (Waters) was used for the assay. The application of this analytical method allows to detect very small amounts of analytes.
6

Sprawozdanie z konferencji naukowej 'Zalozenia reformy prawa w zakresie geoinformacji'

100%
Chromicka A., Gryszczynska A., Szpor G.
Przegląd Geodezyjny
|
2007
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tom 79
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nr 05
18-20
7
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Effect of fertilization on roseroot (Rhodiola rosea L.) yield and content of active compounds

88%
Buchwald W., Mordalski R., Kucharski W. A., Gryszczynska A., Adamczak A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2015
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tom 14
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nr 2
Roseroot (Rhodiola rosea L.) is an important medicinal plant with welldocumented adaptogenic activity. The decrease in its natural resources induces to undertake research on the introduction of this species into cultivation. The aim of the present work was to determine the effect of organic and mineral fertilization on increasing the biomass of underground parts of Rh. rosea and the level of biologically active compounds. Throughout the study period, the highest raw material yield was obtained after the application of the following doses of mineral fertilization: N – 60.0 kg·ha-1, P – 35.2 kg·ha-1, K – 83.0 kg·ha-1, without manure. The use of manure caused a decrease in the average rhizome and root weight by more than 20%. Mineral fertilization application increased the average raw material yield by about 30–40% and allowed obtaining the highest weight of underground organs of the species in question already in the fourth year of cultivation. Furthermore, the present study demonstrates that mineral fertilization does not affect substantially the level of the individual groups of compounds in the raw material, but the use of manure may significantly reduce the content of phenylpropanoids. The level of active compounds is also influenced by crop age. Two-year-old plants were characterized by the highest content of phenylpropanoids as well as by the lowest content of phenylethanoids and phenolic acids. In the following years of cultivation, no statistically significant changes were observed in the level of the analysed groups of compounds.
8

Secondary metabolites in in vitro culture of Virginia mallow (Sida hermaphrodita Rusby)

88%
Mankowska G., Lewanska A., Grygorowicz K., Gryszczynska A., Wielgus K.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
9
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Yield and level of phenolic compounds in the inflorescence of yellow everlasting Helichrysum arenarium (L.) Moench collected from natural sites

88%
Forycka A., Adamczak A., Opala B., Gryszczynska A., Buchwald W.
Herba Polonica
|
2016
|
tom 62
|
nr 4
Introduction: The inflorescence of Helichrysum arenarium (L.) Moench shows anti-inflammatory, antioxidant, detoxifying properties and is traditionally used in liver and biliary tract diseases. Because of its difficult and expensive cultivation, the plant raw material is mainly harvested from natural sites. Objective: The research aimed to determine the local variation in yield and content of flavonoids and phenolic acids in the yellow everlasting inflorescence against the background of the layer structure of vegetation as the rate of plant succession. Methods: The plant raw material was collected from 30 plots of 1 m2, established for three separate populations developing on sandy fallows near Zielona Góra (western Poland). For each study area, percentage cover of the moss-lichen and herb layers, the height, cover and yield of H. arenarium as well as the height and cover of other herbaceous plants were determined. Total contents of flavonoids (expressed as quercetin) and phenolic acids (calculated as caffeic acid) were measured spectrophotometrically, according to Polish Pharmacopoeia. Results: Everlastings reached a cover of up to 70% and the maximum air-dry matter yield of 46.42 g/m2. The height, coverage and yield of H. arenarium were correlated with the parameters describing the herb layer. The content of flavonoids ranged from 0.56 to 0.99%, while that of phenolic acids from 0.82 to 1.80% DM. Conclusions: Yellow everlasting is an important species of early fallows on poor sandy soils and these habitats constitute a rich natural source of herbal raw material. Inflorescences harvested from natural sites are distinguished by a high and similar content of polyphenols and usually meet the requirements of Polish Pharmacopoeia.
10
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Phenolic acids improve the antioxidant activity of ceruloplasmin isolated from plasma of healthy volunteers and atherosclerotic patients

76%
Gryszczynska B., Iskra M., Wielkoszynski T., Malecka M., Budzyn-Napierala M., Kasprzak M., Gryszczynska A.
Journal of Elementology
|
2015
|
tom 20
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nr 1
The aim of the present study was to estimate the ability of individual phenolic acids to eliminate Fe(II) ions from the solution. Moreover, the influence of phenolic acids on the ferroxidase activity of ceruloplasmin (Cp) isolated from blood plasma of healthy volunteers (CpC) and patients with atherosclerosis obliterans (CpAO) was established in vitro. Phenolic acids demonstrated a ferroxidase-like activity, i.e. the ability to eliminate Fe(II) ions, within the studied concentration range of 2.0-17.0 mol 10-5 dm-3, in the following order of decreasing effectiveness: caffeic acid (CA)>ellagic acid (EA)>chlorogenic acid (ChA)>ferulic acid (FA) ≈ p-coumaric acid (PcA) = sinapic acid (SA). The study showed that the ability of phenolic acids to eliminate Fe(II) ions by oxidation or chelation was related to the structure of the former, to the presence of orto -OH groups, especially. Furthermore, the effect of the molar ratio of phenolic acid to Fe(II) ion was observed. EA and ChA, both containing two orto-OH groups and the highest number of –OH groups (4 and 5, respectively), demonstrated the greatest ability to eliminate Fe(II) ions, especially at the Fe(II) to phenolic acid molar ratio of 6:1. Phenolic acids added to samples with a constant amount of Cp caused decrease in the concentration of Fe(II) ions. Therefore, it may be assumed that the addition of phenolic acids to CpC and CpAO, even in low concentrations, caused a significant decrease in the concentration of Fe(II) ions.
11
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Content of alkaloids and flavonoids in celandine (Chelidonium majus L.) herb at the selected developmental phases

76%
Seidler-Lozykowska K., Kedzia B., Bocianowski J., Gryszczynska A., Lowicki Z., Opala B., Pietrowiak A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2016
|
tom 15
|
nr 4
The content of alkaloids and flavonoids and the yield of herb were analyzed in greater celandine cultivar ‘Cynober’ during six following phases: spring rosette formation, the beginning of flowering, full bloom, green fruit, seed harvest, fall rosette formation. Yield of celandine herb was different at the investigated phases and in years of cultivation. The highest yield of herb was observed at the beginning of flowering, then a decrease was noticed, up to the phase of seed harvest, when the yield grown up. The lowest yield of celandine herb was obtained in last phase – fall rosette formation, except 2011 when the lowest yield was in spring rosette formation. The average content of alkaloids was the highest in phase of green fruit (1.097%), while in 2012, the highest content was reached in phase of fall rosette formation – 1.200%. The lowest content of alkaloids was obtained in herb of the beginning of flowering (0.608%) in both years. The mean content of flavonoids was from 0.310% (the beginning of flowering) to 0.522% (seed harvest) and was the same in both years. The stable high content of total alkaloids and flavonoids and individual alkaloids was noticed in phase of fall rosette. Our results suggest that seed maturity is the best time for celandine herb harvest regarding the herb yield and content of alkaloids and flavonoids.
12
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Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

76%
Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.
Herba Polonica
|
2012
|
tom 58
|
nr 3
The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.
13
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Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

76%
Ozarowski M., Pietrowiak A., Gryszczynska A., De A.Chaves D. S., Krajewska-Patan A., Wielgus K., Seremak-Mrozikiewicz A.
Herba Polonica
|
2019
|
tom 65
|
nr 3
14

Cold storage of Tussilago farfara L. nodal cultures

76%
Dreger M., Gorska-Paukszta M., Gryszczynska A., Opala B., Buchwald W., Krajewska-Patan A., Wielgus K.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
15

Effect of yeast elicitor on Salvia przewalskii Maxim. suspension culture

76%
Dreger M., Gorska-Paukszta M., Gryszczynska A., Opala B., Buchwald W., Krajewska-Patan A., Mikolajczak P. L.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
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nr 2
16

In vitro cultures of Tussilago farfara L.

64%
Buchwald W., Krajewska-Patan A., Dreger M., Stanislawska M., Gorska-Paukszta M., Adamczak A., Mirlcarek S., Gryszczynska A.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr Suppl.1
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

64%
Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
|
2012
|
tom 58
|
nr 4
A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.
18
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Determination of lotaustralin in Rhodiola species

64%
Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 2
In our research, the concentration of lotaustralin in the roots of two species Rhodiola kirilowii and Rhodiola rosea were compared. Aqueous and hydroalcoholic extracts from those plants were analyzed too. To determine the content of this compound the ultra performance liquid chromatography – tandem mass spectrometry (UPLC-MS/MS, Waters) was used. The obtained results showed that the content of measured lotaustralin depends on the species of Rhodiola. R. rosea roots are the richer source of lotaustralin then R. kirilowii. The same situation was observed in the extracts. A hydroalcoholic extract from R. rosea contains up to 135.276 mg of lotaustralin in 100 g of dry powdered material. In the case of R. kirilowii extracts, an aqueous extract contained more lotaustralin (74.791 mg/100 g of dry powdered material) then a hydroalcoholic extract.
19
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Content available

Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro

64%
Dreger M., Krajewska-Patan A., Gorska-Paukszta M., Pieszak M., Opala B., Gryszczynska A., Adamczak A., Mikolajczak P. M., Buchwald W.
Herba Polonica
|
2012
|
tom 58
|
nr 4
Tussilago farfara L. (family Asteraceae) is a valuable medicinal plant that has been used as a cough suppressant and as an antibacterial and anti-inflammatory drug. Mucopolysac charides, flavonoids, sterols, phenolic acids and pyrrolizidine alkaloids (PAs) are the main active compounds of coltsfoot. Due to hepatotoxic properties of some pyrrolizidine alkaloids, raw materials that contain PAs should be monitored and determined. The aim of present work was to establish nodal cultures of Tussilago farfara and to determine the content of senecionine and senkirkine in plants propagated in in vitro conditions. Eleven clones of coltsfoot derived from Polish natural populations were established. Rhizome buds were used as explants for the initiation of in vitro cultures on MS (Murashige and Skooge) medium. Every six weeks the shots and leaves were collected and dried. The HPLC method was applied for the identification and determination of senecionine and senkirkine. Content of pyrrolizidine alkaloids varied significantly depending on origin (population). An average sum of alkaloids (senecionine and senkirkine) ranged from 1.23 to 10.47 mg/100g d.w. that corresponds to 0.0013–0.011%, respectively.
20
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Determination of chlorogenic and gallic acids by UPLC-MS/MS

64%
Gryszczynska A., Opala B., Lowicki Z., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Boron D., Bogacz A., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 1
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.
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