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Recommendations: diagnosing tick-borne diseases in a laboratory - meeting research quality standards and providing patient safety

100%

Puacz E., Smutek M.

Health Problems of Civilization

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2017

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tom 11

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nr 2

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Alcohol drinking under intermittent access in group-housed C57BL/6J mice

80%

Smutek M., Rodriguez-Parkina J., Turbasa M., Przewlocki R.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr Suppl.1

Models of alcohol drinking in rodents are useful in determining factors underlying uncontrolled alcohol abuse. Here we describe a new model, which overcomes a limitation of previous approaches, the necessity of studying animals in isolation. Over the course of 4–5 weeks group-housed mice intermittently received free or instrumental access to 12% alcohol in the IntelliCage system. Animals developed stable alcohol preference which was similar across 6 cohorts tested (46.6 ± 12.8%). Compared to behaviors of singlehoused mice, we found no escalation of drinking over time, and no difference in alcohol preference between males and females, but comparable levels of alcohol preference to those previously reported. Motivation to obtain alcohol or saccharin measured under a progressive ratio schedule was initially similar, but the breakpoint decreased over consecutive sessions in case of alcohol (from 19.8 to 15.9). Conversely, addition of 0.03% quinine had a smaller impact on alcohol than saccharine intake (72.2% vs. 97.2% decrease). We observed that sequences of animal entries to alcohol-containing corners diverged from random distribution. Thus, this model applied to group-housed mice induces stable levels of alcohol drinking and allows to measure its motivational aspects as well as explore relations between social structure and drinking behavior.

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Molecular and behavioral patterns associated to stress responsivity and PTSD risk

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Szklarczyk K., Smutek M., Korostynski M., Solecki W., Golda S., Przewlocki R.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

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nr S

Post-traumatic stress disorder (PTSD) is a chronic anxiety condition that develops as a result of a terrifying event. Clinical studies show that only about 10% of trauma-exposed people suffer from PTSD. Our research was focused on endophenotypes and molecular biomarkers of PTSD in an animal model. Differences in response to stress among inbred mouse strains (C57BL/6J, DBA/2J, SWR/J and 129P3/J) were compared: a single intense footshock was applied and ultrasonic vocalization during/after the stress was measured. Long-lasting effects were assessed 4-6 weeks after the traumatic event: conditioned and sensitized fear, social withdrawal, depressive-like behavior and susceptibility to drug addiction. SWR/J strain displayed the lowest conditioned fear, whereas sensitized fear was increased over time in C57BL/6J mice. Moreover, C57BL/6J strain exhibited increase in depressive-like behavior, while DBA/2J strain displayed increased social withdrawal. In addition, it was observed that exposition to traumatic stress increased sensitivity to rewarding properties of morphine in 129P3/J mice. Diverse long-lasting behavioral consequences of exposition to stress were associated with changes in basal and stress-induced profile of glucocorticoid receptor-dependent (GR) genes (e.g., Fkbp5 and Tsc22d3) in amygdala. Furthermore, our research showed that administration of GR antagonist disrupted reconsolidation of the traumatic event memory. Our research supports a model in which genetic factors are important for phenotypic variation in responsivity to stress. These genes may provide novel insight into mechanisms of stress-related disorders. This work was supported by Polish MSHE grants NN405 274137, N405 143238, IUVENTUS Plus and POIG De-Me-Ter 3.1

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

1 Health Problems of Civilization

3 Smutek M.

2 Przewlocki R.

1 Golda S.

1 Korostynski M.

1 Puacz E.

1 Rodriguez-Parkina J.

1 Solecki W.

1 Szklarczyk K.

1 Turbasa M.

1 2017

1 2013

1 2011

Recommendations: diagnosing tick-borne diseases in a laboratory - meeting research quality standards and providing patient safety

Alcohol drinking under intermittent access in group-housed C57BL/6J mice

Molecular and behavioral patterns associated to stress responsivity and PTSD risk