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1

Zdolność oocytów klaczy do dojrzewania i zapłodnienia in vitro

100%
Mlodawska W.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 01
The aim of the review was to present current views on the factors influencing the in vitro maturation (IVM) and fertilization (IVF) of mare oocytes. The first two foals produced with the use of the so-called standard IVF (co-incubation of oocytes and spermatozoa in culture media) were born over 20 years ago. To date, it has been possible to obtain offspring in horses after the fertilization of in vitro matured oocytes by the intracytoplasmic sperm injection technique (ICSI) or by the surgical transfer of oocytes to the oviducts of inseminated mares (fertilization in vivo). Causes of the low efficiency of IVF in horses are complex and may be related to an incomplete maturation of oocytes, an inappropriate method of sperm capacitation in vitro, as well as the use of non-compliant media for the development of inseminated oocytes and/or early embryos. The paper describes the method of oocyte collection from mare ovaries and the most important factors influencing the number and quality of oocytes obtained. It discusses the relationship between the physiological status of ovarian follicles, cumulus oophorus morphology and the capacity of mare oocytes for in vitro maturation and fertilization. In addition, some aspects of nuclear and cytoplasmic maturation of equine oocytes are presented. The understanding of the in vivo maturation mechanisms of equine gametes and of the developmental requirements of embryos helps to improve culture conditions and the in vitro fertilization efficiency of equine oocytes.
2

Zmiany w jajnikach podczas dojrzewania płciowego klaczy

100%
Mlodawska W.
Prace Komisji Nauk Rolniczych, Leśnych i Weterynaryjnych. Polska Akademia Umiejętności
|
2014
|
tom 20
3

Dojrzewanie płciowe klaczy i perspektywy skracania okresu międzypokoleniowego u koni

63%
Mlodawska W., Tischner M.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 07
The aim of the study is to present current knowledge on the mechanisms regulating puberty in mares and the possibility of shortening the intergenerational period in horses through modern animal reproduction biotechnology. The study discusses fetal sex recognition in horses by means of ultrasound, pre- and postnatal development of mare gonads, oogenesis and folliculogenesis, as well as the process of selection and elimination of oocytes. It also describes the role of gonadotropins, ovarian hormonal activity and morphological changes occurring during sexual maturation. It has been shown that about 37% of mares attain sexual maturity in the first year of life. It has also been documented that one-year and two-year-old fillies produce normal embryos that can be used for transplantation and give offspring. It has also been proved that embryos can be produced in vitro from oocytes of juvenile mares. There is hope that acquiring preantral follicles from the ovary and their in vitro culture until the oocyte reaches full maturity for fertilization will permit us to obtain embryos and offspring from mares, including those sexually immature. These methods, combined with in vitro fertilization and embryo transfer techniques, have already made it possible to obtain normal embryos and even live-born offspring in other mammals.
4

Wpływ komórek ziarnistych i pożywek hodowlanych na kompetencje mejotyczne oocytów bydła oraz ich zdolność do rozwoju po zapłodnieniu in vitro

63%
Mlodawska W., Ploszaj E.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 05
The aim of the study was to compare the effect of gonadotropic hormones and granulosa cells on the maturation and developmental capacity of cattle oocytes in vitro, as well as the effect of TCM 199 and DMEM/F12 media on the development of embryos obtained in co-culture with oviduct epithelial cells. Fertilization was performed with the use of frozen semen from 2 bulls. Twenty hours after insemination, presumptive zygotes were placed in co-culture with oviduct cells in a TCM 199 (TCM-KJ co-culture) or a DMEM/F12 medium (DMEM-KJ co-culture) and cultured for 7-9 days. Metaphase II was reached by 40% and 48% of oocytes cultured in the presence of granulosa cells and gonadotropins, respectively. Only embryos obtained from oocytes maturing in the presence of granulosa cells developed to the blastocyst stage. Considerably more dividing embryos were obtained when the presumptive zygotes were co-cultured with TCM-KJ (38.1%) rather than with DMEM-KJ (8.6%; P < 0.01). This study showed that the presence of granulosa cells had no effect on the nuclear maturation of cattle oocytes, but increased their capacity for embryonic development. TCM 199 is much more useful than DMEM/F12 for the co-culture of cattle embryos with oviduct cells.
5

I Europejska Konferencja nt. gamet koni

63%
Mlodawska W.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 02
134-135
6

Metody pozyskiwania oocytow z pecherzykow jajnikowych klaczy oraz ich hodowla w warunkach in vitro

51%
Mlodawska W., Slonina D., Okolski A.
Acta Agraria et Silvestria. Series Zootechnica
|
1995
|
tom 33
65-73
7

Dojrzewanie in vitro oocytow cielat i krow

51%
Mlodawska W., Tischner M., Polanska M.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 03
297-300
8

Przyżyciowe pozyskiwanie oocytów z przedowulacyjnych pęcherzyków jajnikowych klaczy

51%
Okolski A., Duchamp G., Tischner M., Mlodawska W.
Medycyna Weterynaryjna
|
1990
|
tom 46
|
nr 05
Mares were prepared for oocyte recovery by estrus syn­chronization with PGF2a and HCG. Oocytes from 12 mares were recovered after 36.7 hours since HCG injection. The oocytes were obtained after manual location of the ovaries through the rectum and puncture the follicle through the ventral integument in the flank area. The follicle fluid was aspirated into a syringe connected by a plastic tubing with a needle approx. 15 cm long. Out of six oocytes recovered (50%) five were morphologically normal.
9

Recovery of equine oocytes, their developmental stage in relationship to ovarian follicle size, and their maturation in vitro

51%
Mlodawska W., Okolski A., Lietz W.
Acta Agraria et Silvestria. Series Zootechnica
|
1991
|
tom 29
10

Ocena konfiguracji chromatyny jadrowej w dojrzewajacych in vitro oocytach klaczy

51%
Mlodawska W., Wnuk A., Okolski A.
Roczniki Naukowe Zootechniki. Suplement
|
2005
|
tom 22
|
nr 1
249-252
11

Twardosc oslonki przejrzystej oocytow klaczy w warunkach hodowli in vitro

51%
Mlodawska W., Okolski A., Polak I.
Medycyna Weterynaryjna
|
1999
|
tom 55
|
nr 12
836-838
12

Selected methods of in vitro embryo production in felids - a review

33%
Prochowska S., Nizanski W., Partyka A., Kochan J., Mlodawska W., Nowak A., Migdal A., Skotnicki J., Grega T., Palys M.
Animal Science Papers and Reports
|
2017
|
tom 35
|
nr 4
During the past decade the need for Artificial Reproductive Techniques in felids has greatly increased. Mostly, this is a result of growing expectations that these techniques may be applied in conservation biology and thereby contribute to saving wild felids from extinction. In this article we describe three most common methods of obtaining embryos in vitro in the domestic cat and its wild relatives: classic in vitro fertilisation, in vitro fertilisation by intracytoplasmic sperm injection and somatic cell nuclear transfer. Each of the methods provides a cleavage rate of around 50% and approx. 20% of embryos develop to the blastocyst stage. After the transfer of embryos produced by these methods, scientists obtained living offspring of the domestic cat, as well as several wild cats: the tiger, serval, fishing cat, caracal, ocelot, wild cat, sand cat, black-footed cat and the oncilla. These successes, in spite of the low efficiency of the discussed methods, are promising and suggest that biotechniques of reproduction will be valuable tools in the protection of wild species. Somatic cell nuclear transfer will allow to sustain the narrow gene pool in the critically endangered felids. For these reasons it is necessary to conduct further research on the optimization of artificial reproduction techniques in cats.
13

ARTs in wild felid conservation programmes in Poland and in the world

33%
Kochan J., Nizanski W., Moreira N., Cubas Z. S., Nowak A., Prochowska S., Partyka A., Mlodawska W., Skotnicka J.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 3
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