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Influence of organic supplements on accumulation of beta-boswellic acid, 11-keto-beta-boswellic acid and acetyl-11-keto-beta-boswellic acid in callus culture of Boswellia serrata Roxb.

100%

Ghorpade R., Chopra A., Nikam T.

Acta Physiologiae Plantarum

2012

tom 34

nr 6

Boswellia serrata Roxb. is a source of several bioactive triterpenoids. Boswellic acid, obtained from oleogum resin of the tree, is a major potentially bioactive and medicinal compound. Unrestricted exploitation of its natural resource has led to its listing among the threatened and endangered species. Accumulation of the compound through tissue culture seems a promising option. The present work was conducted to study the effect of sodium pyruvate, L-phenylalanine, glycine, ferulic acid and sucrose on the growth of callus and accumulation of four principal isomers of boswellic acids, viz. β-boswellic acid (BBA), acetyl-β-boswellic acid (ABBA), 11-keto-β-boswellic acid (KBBA) and acety1⁻¹1-keto-β-boswellic acid (AKBBA). Callus cultures obtained from embryo explants of Boswellia serrata on Murashige and Skoog medium containing 2.5 µM 6-benzyladenine, 15 µM indole acetic acid and 200 mg 1⁻¹ polyvinyl pyrrolidone was supplemented with varying concentrations of the supplements. Sodium pyruvate was most beneficial for the production of AKBBA (77 folds), BBA (27 folds) and ABBA (27 folds) at 10 mg 1⁻¹ and for KBBA (47 folds) at 5 mg 1⁻¹ when compared with control. It was closely followed by sucrose (50 g 1⁻¹) resulting in KBBA (22-fold), AKBBA (25-fold), BBA (17-fold) and ABBA (10-fold). Glycine, L-phenylalanine and ferulic acid were relatively less effective. It can be concluded that callus cultures manipulated with different concentrations of organic supplements, sodium pyruvate or sucrose, in particular, could be considered as an alternate strategy for direct production of boswellic acid and help in the conservation of the species.

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Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle

88%

Chopra A., Gupta I. D., Verma A., Chakravarty A. K., Vohra V.

Polish Journal of Veterinary Sciences

2015

tom 18

nr 3

Lactoferrin (Lf) gene promoter was screened for the presence of single nucleotide polymphism in indigenous and crossbred cattle from North India and to evaluate its association with Mastitis. Study revealed the presence of genetic variation in regulatory region of bovine Lactoferrin gene using PCR-RFLP technique. Three genotypes namely GG, GH and HH were identified. A single nucleotide change, from guanine to adenine at 25th position was found to be significantly associated (p<0.05) with clinical mastitis in indigenous Sahiwal and crossbred Karan Fries cattle maintained at organised herd of National Dairy Research Institute, Karnal. A non-significant association was observed between subclinical mastitis, somatic cell score (SCS), and GG genotype in Karan Fries cattle, however, a lower SCS was observed in animals having GG genotype. Overall a lower incidence of clinical mastitis was recorded in those animals having GG genotype of Lf in Sahiwal and Karan Fries (KF) cattle. The SNP identified in the promoter region may effect expression lactoferrin protein, which may lead to different levels of antibacterial and anti- nflammatory activity of Lf gene. Results from this study indicated the probable role played by Lactoferrin promoter to serve as candidate gene for mastitis susceptibility among indigenous and crossbred milch cattle.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Polish Journal of Veterinary Sciences

2 Chopra A.

1 Chakravarty A. K.

1 Ghorpade R.

1 Gupta I. D.

1 Nikam T.

1 Verma A.

1 Vohra V.

1 2015

1 2012

Wyniki wyszukiwania

Influence of organic supplements on accumulation of beta-boswellic acid, 11-keto-beta-boswellic acid and acetyl-11-keto-beta-boswellic acid in callus culture of Boswellia serrata Roxb.

Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle