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1
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Tworzenie kalusa i organogeneza z liscieni papryki w kulturach in vitro

100%
Gatz A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
1994
|
tom 414
379-386
Na eksplantatach liścieniowych Capsicum annuum L., cv. Bryza auksyna (IAA) indukowała tworzenie tkanki kalusowej oraz korzeni. Natomiast cytokinina (BAP) stymulowała nie tylko wzrost kalusa, ale również przy najwyższym stężeniu, tworzenie pąków przybyszowych. Dla wydajnego tworzenia pąków jak i korzeni niezbędna jest obecność obu regulatorów wzrostu. Nie wykazano większego wpływu czynnika endogennego (część dystalna i proksymalna liścienia) na zdolności regeneracyjne.
2
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Morpho-histological aspects of adventitious shoot formation without plant growth regulators in seed explants of Capsicum annuum L., and impact of preculture on regeneration

100%
Gatz A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 2
The physiological state of plant material is the crucial endogenous factor at the explant choice for plant regeneration. The phases of germination characterised by various, following each other biochemical and developmental processes can affect the organogenesis capability. This research examined the morphological and anatomical events during the early stages of organogenesis and plant regeneration in explants derived from seeds of Capsicum annuum L., cv. Bryza preincubated under high humidity conditions from 0 to 6 days and next cultured on MS medium without PGRs. The early stage of de novo shoot formation reminded leaf differentiation in planta. First the leaves began to differentiate as spherical and tongue shaped structures from epidermis and subjacent layers of the explants about the 7th day of culture. In some cases nearly at the base of previously formed leaf and even on its petiole one or two leaves as well as shoot apex in their axils were induced thereby forming young shoot which underwent elongation and whole plant regeneration after 2 subculturing. More advanced developmentally structures of adventitious shoot were obtained while prolonging preculture duration. This was the favourable effect on the shoot differentiation, their elongation and plant regeneration as seed submitted preculture for 3, 4, 5 days however, seeds not treated with preculture revealed the best response as regard to shoot primordium formation at the earliest stage.
3

Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper [Capsicum annuum L., cv.Bryza] in vitro

100%
Gatz A.
Acta Societatis Botanicorum Poloniae
|
2002
|
tom 71
|
nr 3
4

Histological changes during the adventitious shoot formation in seedling explants of pepper [Capsicum annuum L.] cultured in vitro

100%
Gatz A.
Acta Societatis Botanicorum Poloniae
|
2002
|
tom 71
|
nr 4
5

Wplyw IAA i BAP oraz warunkow swietlnych na roznicowanie kalusa papryki in vitro

63%
Gatz A., Reinke M.
Biotechnologia
|
2004
|
nr 2
176-184
6

In vitro organogenetic potential of cotyledon and leaf explants of Capsicum annuum L.,CV.Bryza

63%
Gatz A., Rogozinska J.
Acta Societatis Botanicorum Poloniae
|
1994
|
tom 63
|
nr 3-4
255-258
7
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Tracheary element differentiation and morphogenetic changes in callus derived from embryos of pepper (Capsicum annuum L.)

63%
Gatz A., Kowalski T.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2011
|
tom 10
|
nr 1
Tracheary elements appear already in the early stages of in vitro organogenesis and precede the formation of meristemoids and shoot primordia which further develop into shoots. Thus this process can be considered as an important indicator of potential, organogenic capability of the explants. The aim of this study was to investigate the effect of auxin (IAA), cytokinin (BAP), sucrose and the light conditions (light with 16-h photoperiod, darkness) upon de novo differentiation of tracheary elements (TEs) in callus derived from mature embryos of pepper cv. Bryza. Moreover, it was to determine the proliferation ability of the callus cells and changes of callus morphology. TEs were differentiated in the form of single cells, irregular clusters, strands and whirls exclusively with reticulate and pitted thickening of the secondary wall and the clusters occurred the most frequently. Cytokinin used alone, as well as in auxin combination, stimulated xylogenesis with the highest efficiency as well as with regard to the frequency and TE cell number within clusters. The highest number of TEs per cluster was obtained in the case of combination 0.1 mg·dm⁻³ IAA + 0.1 mg·dm⁻³ BAP on the light. Smooth surface, large cohesion cell degree and green colour are the morphological callus features accompanied by the effective TE formation.
8

In vitro morphogenesis of callus cells of Capsicum annuum [L.] from suspension cultures

63%
Gatz A., Lewicka E.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
9

Plant regeneration from seed and seedling explants of pepper cv. bryza via organogenesis

51%
Gatz A., Drozdowska L., Rogozinska J.
Biological Bulletin of Poznań. Supplement
|
1995
|
tom 32
10

Histological analysis of organogenesis and somatic embryogenesis during shoot formation in sugar beet (Beta vulgaris L.) via gynogenesis

51%
Tomaszewska-Sowa M., Figas A., Gatz A.
Polish Journal of Natural Sciences
|
2017
|
tom 32
|
nr 4
The aim of the present research were histological analysis of regenerating structures through in vitro gynogenesis from unfertilized ovules of sugar beet (Beta vulgaris L.). The process of shoot regeneration using a novel two stage method combines the preculture in liquid medium with the culture on solid medium. The highest number of explants that formed shoots (60%) was observed on medium supplemented with BAP, sucrose and gerlite, and as regards carbohydrates used in the medium most of explants forming shoots (42%) and the largest total number of shoots (110) was observed for glucose. To accurately determine the course of shoot formation, histological analyses were performed. Careful histologic evaluation of regenerating structures revealed the presence of numerous meristematic centres. In some meristems formation of specialized tissues and organs was observed, including epidermis, apical meristem, leaf primordia and tracheal elements. The analyses showed that the regeneration of the new structures from sugar beet ovules occurred both through organogenesis as well as somatic embryogenesis since the presence of somatic embryos in the globular stage or torpedo stage were observed.
11

Bioaccumulation of heavy metals in herbal plants from areas not exposed to heavy anthropopressure

45%
Kobierski M., Tomaszewska-Sowa M., Figas A., Gatz A., Sawilska A. K.
Polish Journal of Soil Science
|
2017
|
tom 50
|
nr 1
The aim of the study was to evaluate the content of Zn, Cu, Mn, Pb, Hg and Fe in sandy everlasting, yarrow and stinging nettle in relation to the concentration of metals in the soil. Samples of soils and plants were collected from natural habitats (edges of forests in the Kujawy-Pomerania Province). The total metal content and their available forms for plants in the soil samples were determined. The stinging nettle inhabited the richest environmental areas in which anthropogenic accumulation of metals in the surface of soils was determined. The investigated soils were not contaminated with heavy metals and the content of their plant-available forms was not harmful for a proper plants growth. The content of metals extracted with the diethylenetriaminepentaacetic acid was considerably higher than the concentration referred to as the deficit level for plants. Among the analyzed herbal plants, sandy everlasting contained the largest amounts of copper, manganese, and only concentration of lead in dry weight was higher than 10 mg⋅kg⁻¹, indicating that the plants harvested from the study areas should not be used in herbal medicine. Bioconcentration factor (BCF) values point clearly to the mercury and zinc accumulation in the aboveground parts of herbal plants.
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