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Serological and bacteriological examinations for the presence of Salmonella sp. were carried out on 104 samples of fermented sludge sediments, both fresh and dewatered on plots. Serological examinations were performed with the use of the latex test. Bacteriological tests were done on growth media and differential ones. Biochemical examinations were carried out as well. Seropositive results were noticed in 64.1 per cent of fresh fermented sludge sediments, dewatered on plots. Salmonella sp. were isolated from 32.6 per cent of the samples of fresh fermented sediments and from 15.4 per cent of the samples of fermented, dewatered sediments. The compatibility between serological and bacteriological examinations was 90.3 per cent and was statistically significant.
Recreational areas cover parks, bicycle paths, lawns, urban squares, sports complexes, holiday camp areas, playgrounds for children, beaches, and even spontaneously used green field open spaces. While using recreational areas, people take with them accompanying animals (dogs, cats). These animals constitute the main source and reservoir of many dangerous zoonoses, including parasitoses caused by roundworms of the genus Toxocara and Trichuris. The objective of the presented study was determination of the level of contamination of soil with parasites’ developmental forms (eggs of Toxocara spp., Ascaris spp., Trichuris spp and Ancylostoma) in selected recreational areas in the Lublin Region of southeastern Poland. The material for the study was collected from beaches in recreational areas located around the Białe Lake near Włodawa, the water reservoirs in Krasnobród and Janów Lubelski. The studies were carried out from May-October 2010 durng which time a total number of 215 samples of sand were collected. Eggs of Ascaris spp. and Trichuris spp. were detected based on the Polish Standard PN-Z-19000-4/2001. Beaches on the Białe Lake were the most contaminated. The eggs of intestinal parasites were isolated from 6 per 15 samples examined, which is 40%. In one sample, the presence of the eggs of 3 types of parasites were observed, while the eggs of Ancylostoma were not found in any of the samples. Attention should also be paid to the possibility of contamination of recreational areas with the eggs of intestinal parasites by wild animals. Recently in Poland, as well as in many other European countries, the phenomenon of synantropization of wild living animals has been observed, which most frequently concerned wild boar (Sus scrofa) and red fox (Vulpes vulpes).
The objective of this study was to evaluate the contamination of vegetables, fruits and soil with zoonotic parasite eggs on organic and conventional farms in south-eastern Poland. To evaluate the contamination with eggs of zoonotic parasites, examinations were conducted on 8 conventional and 11 organic farms in south-eastern Poland from May – October in 2008 and 2009. The following fruit and vegetables were selected for the experiment: strawberry, leek, onion, carrot, zucchini, beetroot, parsley, potatoes, celery, rhubarb, lettuce, cabbage, broccoli, pumpkin, young beetroot leaves, cauliflower, French beans, turnip, fennel and sorrel. A total of 187 samples of vegetables, fruits and soil were examined by means of a modified flotation method according to Quinn et al. (1980). Contamination with Ascaris, Trichuris and Toxocara eggs was found, with a higher number of positive samples revealed on conventional (34.7%), compared to organic farms (18.9%). The level of contamination in soil samples from conventional farms was higher (88.5% positive samples), than of those from organic farms (32.8%). Of the 15 geohelmints eggs, positive samples were found in vegetables: 9 Toxocara eggs, 4 Ascaris eggs and 2 Trichuris eggs. No geohelmints eggs were observed in the strawberry samples. The consumption of vegetables and fruits contaminated with the eggs of parasites may be the cause of parasitoses in humans. Stricter sanitary standards on farms of all types may limit the incidence of parasitic zoonoses.
Wprowadzenie i cel pracy. Jady i trucizny w świecie zwierzęcym stanowią broń chemiczną Jadowite i trujące bezkręgowce wytwarzają trucizny w celach obronnych lub w celu zdobycia pożywienia. Zwierzęta te wykształciły morfologiczne i fizjologiczne przystosowania (narządy i gruczoły jadowe), które umożliwiają dostanie się jadu lub trucizny do ciała lub na ciało innego zwierzęcia lub człowieka. Substancje te wywołują u człowieka schorzenia narządowe, reakcje alergiczne i toksyczne, niekiedy nawet prowadzące do śmierci. Znajomość miejsc bytowania i sposobów zachowania się tych zwierząt może uchronić człowieka przed niebezpieczeństwem utraty zdrowia, a nawet życia. Celem pracy jest przedstawienie wybranych gatunków zwierząt trujących i jadowitych, należących do bezkręgowców, groźnych dla zdrowia lub życia człowieka. Opis stanu wiedzy. W pracy przedstawiono gatunki najbardziej niebezpieczne dla człowieka, których jad jest śmiercionośny. Do jadowitych bezkręgowców zaliczamy: parzydełkowce, skorpiony, pająki, mięczaki (ślimaki, głowonogi). Przykładem są: krążkopławy z rodzaju Chironex, karakurt (Latrodectus mactans tredecimguttatus). Do trujących bezkręgowców należą miedzy innymi niektóre motyle (monarcha – Danaus plexippus) i chrząszcze (majka lekarska – Lytta vesicatoria). Podsumowanie. Wiedza na temat zwierząt jadowitych i trujących jest bardzo przydatna dla osób wybierających się do krajów tropikalnych. Niestety, jest ona nadal niewystarczająca zarówno wśród turystów, jak również pracowników biur podróży.
The objective of the study was to determine the degree of municipal wastewater contamination with intestinal parasite eggs of the genera Ascaris, Toxocara, and Trichuris at individual stages of treatment, and indication of potentially weak points in the hygienisation of sewage sludge. The study was conducted in 17 municipal mechanical-biological wastewater treatment plants which, to a slight degree, differed in the technological process of wastewater treatment and the method of hygienisation of sewage sludge. The selected treatment plants, located in seven regions, included five classified as large agglomerations (population equivalent - PE >100 000), ten as medium-size (PE 15 000-100 000), and two as smaller size with PE 10 000 - 5000. The largest number of viable eggs of Ascaris spp., Toxocara spp., and Trichuris spp. was found in the sewage sludge collected from the priman settling tank. A slightly lower number of the eggs were found in the samples of excess sludge, which indicates that the sedimentation process in the priman settling tank is not sufficiently long to effectively separate parasites' eggs from the sewage treated. The number of eggs of Ascaris spp. and Toxocara spp. in the fermented sludge was nearly 3 times lower than that in the raw sludge. The effectiveness of hygienisation of dehydrated sewage sludge by means of quicklime was confirmed in two wastewater treatment plants, with respect to Ascaris spp. eggs, in three plants with respect to Toxocara spp. eggs, and in one plant with respect to Trichuris spp. eggs. The mean reduction of the number of eggs was 65%, 61%, and 100%, respectively. In one wastewater treatment plant, a reduction in the number of v iable eggs of Ascaris and Trichuris species was also noted as a result of composting sludge by 85% and 75%, respectively. In the remaining treatment plants, no effect of hygienisation of sewage sludge was observed on the contents of viable eggs of these nematodes.
The aim of the study to evaluate the occurrence of intestinal parasites and Salmonella in fox and mink faeces and to assess potential biological risk related to their use as a natural fertilizer in agriculture. The study included 12 randomly selected fox-breeding farms with a total population of 2800 animals and 9 mink farms with a total of 24 650 animals. The material examined comprised 120 samples of fox faeces and 66 samples of mink faeces. In addition, the intestines of 45 foxes from 3 farms were examined by the SCT method. The samples of feces and intestines of slaughtered foxes were examined parasitologically and bacteriologically. In 13 samples of fox faeces, the presence of parasites of the genus Isospora was observed, whereas 2 samples contained the eggs of nematodes of the alimentary tract of Toxocara canis and Strongyloides spp. The examination of the intestines confirmed the presence of mature forms of Toxocara canis in 2 foxes. In mink, only parasites of the genus Isospora were found. The presence of pathogenic bacteria was noted in the faeces of foxes. In 5 samples of fox faeces, Salmonella were found. No Salmonella were isolated in mink faeces. The present study confirmed a considerable decrease in the extensiveness of Toxocara spp. invasion in foxes bred on farms in Lublin Voivodeship. At the same time, it was confirmed that farm-raised foxes, unlike mink, are not entirely free of intestinal parasites and Salmonella, which highlights the need for continued monitoring.
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