Cruciferin was separated from the rapeseed crude proteins using salting out with ammonium sulphate and Sephadex G-200 gel filtration. Then, so obtained protein fraction was separated using a micellar electrokinetic chromatography (MEKC) with SDS as a the surfactant. Nine peaks with migration times between 14.33 and 20.48 min were recorded on the chromatogram. The main cruciferin subfractions were characterised with molecular mass of 22 000 and 33 000 determined using MEKC technique. UV spectra showed that cruciferin protein appears as a complex with phenolic acids.
Starch was extracted from rice bean (Vigna umbellata L.) using 0.5% (w/v) NaOH solution for 24 h at 60°C. The separated starch was characterised for the content of moisture, ash, nitrogen, lipids, amylose, starch damage, enzymatic (α-amylase) and acid (2.2 mol/L HCl) hydrolysis, swelling factor, amylose leaching, water and fat absorption, and gelatinization temperature. The gross chemical composition of rice bean starch did not differ significantly from other edible legumes, for example, amylose content was 32.8%. The rice bean starch was found to absorb 83.2% of water, 77.6% fat and exhibit a gelatinization temperature of 66±2°C.
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