Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 67

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 4 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 4 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Global situation of foot-and-mouth disease (FMD) - a short review

100%
Kesy A.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 4
This article reviews the actual world FMD situation. In 2000, fifty nine countries officially reported outbreaks of FMD. The disease occurred in Europe (Greece), Asia (Russia, Mongolia, Bangladesh, Cambodia, China, Japan, Laos, Nepal, Pakistan, Philippines, Republic of Korea, Taiwan, Thailand, Vietnam, Iran, Iraq, Turkey, in Caucasian region - Georgia, Azerbaijan and Armenia as well as in Kazakhstan, Kyrgyzstan, Turkmenistan and Tajikistan), Africa (Egypt, Kenya, Mauritania, South Africa, Tanzania, Uganda, Malawi, Namibia, Zambia and Zimbabwe) and in South America (Brazil, Colombia, Uruguay, Bolivia, Peru, Ecuador and Venezuela). In 2001, FMD was still spreading throughout the endemic regions and appeared in some of the west European countries - Great Britain, the Netherlands, France and Ireland. In South America, FMD occurred in Argentina, Uruguay, Brazil and Colombia. In Asia the FMD spread in Turkey, Iran, Afghanistan, Georgia, Azerbaijan, Mongolia, Kuwait, Bahrain, Yemen, Qatar, United Arab Emirates, Oman, Iran, Bhutan, Nepal, Malaysia, Philippines, Thailand and Taiwan. The FMD situation in Africa was unclear, but probably most countries in West, East and South Africa were affected. The most recent data of the OIE from May 2002 confirmed FMD outbreaks in population of pigs in Republic of Korea.
2

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

63%
Paprocka G., Kesy A.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cell culture growth. After about 23 h and while still in the growth phase, the cells were infected with decimal virus dilutions (10⁻¹-10⁻⁶) containing from 100 000 to 1 median tissue culture infectious doses (TCID₅₀). SVDV replication in cell cultures induced a change in cell index; together with the occurrence of cytopathic effect (CPE), the CI values declined. A significant correlation between the concentration of the virus used and CPE occurrence was found. The results also enabled determination of cell sensitivity to SVDV infection. The highest sensitivity was exhibited by IB-RS-2, followed by SK-6. To conclude, the xCELLigence System was used effectively and evaluated as being an efficient tool for CPE detection and SVDV replication analysis in cell cultures. Compared to the standard method, it enabled a more precise assessment of viral replication based on the quantitative CI measurement, providing additional current information.
3

Wirus choroby niebieskiego jezyka - budowa i replikacja

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 09
597-600
The article reviews the structure of BTV and the role of capsid proteins in the replication process. The BTV is an icosahedral virus with a genome of approximately 19 200 base pairs and composed of ten linear segments of double-stranded RNA (dsRNA). These RNA segments are packaged in a triple layered protein capsid (88 nm in diameter). The outer shell of capsid is composed of two structural proteins: 60 trimers of VP2, which acting as a hemagglutinin (HA) is responsible for glycoprotein receptor binding, and 120 trimers of VP5 that mediate the release of viral particles from endosomal compartments into cytoplasm and undergo pH dependent conformational changes that allow membrane fusion and syncytium formation. The intermediate layer consists of VP7 - protein the major immunodominant of BTV. This layer surrounds the subcore (54 nm) which consist of 12 decamers of the VP3 protein (role in the structural integrity of the virus core) and three minor structural proteins: VP1 (replicase), VP4 (mRNA capping enzyme) and VP6 (RNA-dependent ATPase and helicase). Moreover, the viral subcore consists of nonstructural proteins: NS1, NS2, NS3 and NS3A, which participate in the control of BTV replication, maturation and export from infected cell.
4

Metody polimeryzacji DNA in vitro w diagnostyce choroby niebieskiego języka

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 03
5

Seroprevalence of antibodies against bluetongue virus in animals imported to Poland from EU countries

63%
Niedbalski W., Kesy A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 3
205-208
The aim of this study was to determine the seroprevalence of BTV-specific antibodies in animals imported to Poland from EU countries after 15 June 2006. From 1 January 2007 to 22 January 2008, a total of 10719 samples of sera collected from cattle, goats and fallow deer were tested. Sera were screened using the highly sensitive and specific c-ELISA test and positive results were confirmed by the AGID assay. Out of 10719 sera, 30 (0.28% of the total number of samples) were found to be positive in both tests applied. All of 21 seropositive cattle specimens were imported to Poland from Germany whereas 9 seropositive fallow deer were of Dutch origin. In conclusion, it can be stated that because BTV situation in Europe is getting worse, implemented surveillance studies should be continued to monitor the actual BT status in Poland.
6

Epidemiologia choroby niebieskiego jezyka w Europie

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 11
739-742
Probiotic products are representatives of functional food, which constitutes a dynamically growing segment of the food market. Functional food is defined as all the products that demonstrate beneficial effects on human health surpassing that resulting only from the presence of nutrients traditionally recognized as essential. Probiotics are selected microorganisms that are also called bioactive compounds of food products. Up until the present, the major branch of the food industry in which probiotics have been applied is the milk industry. The most typical active components of probiotic products are lactic acid bacteria, particularly Lactobacillus species, and bifidobacteria. Health claims of probiotics are numerous, but include maintenance of healthy intestinal flora (e.g. as a result of their competition for the nutrients and/or the production of antimicrobials inhibiting the growth of undesirable microflora and pathogens) and the stimulation of the immune system. Research and industry experiments on the application of probiotic bacteria in meat processing have proved that raw fermented sausages are the most suitable environment for their growth. In the meat industry, like in other food industry branches, a key criteria for the selection of appropriate probiotics strains are as follows: their safety (non-pathogenicity), growth properties during processing or resistance to changing parameters of technological processes as well as characteristics that enable the attainment of the expected quality, including the desired sensory attributes of final products. The article presents the most important properties and criteria of the selection of suitable probiotic bacteria for food and problems concerning the application of probiotics in meat processing.
7

Pryszczyca - wciąż aktualne zagrożenie

63%
Paprocka G., Kesy A.
Magazyn Weterynaryjny
|
2011
|
tom 20
|
nr 02
8

Patologia i patogeneza choroby niebieskiego języka

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 07
s.459-563,rys.,bibliogr.
The article reviews the clinical symptoms and pathogenesis of Bluetongue virus (BTV) infection of domestic and wild ruminants. The clinical signs of BTV infection occur principally in sheep but typical BT signs have also been observed in cattle infected with BTV serotype 8 in North-Western Europe. BTV infection can display a variety of clinical manifestations, ranging from subclinical or mild, to acute or even fatal infections. The lesions of BT differ not only among the animal species but also within breeds of the same species. In sheep the febrile period (41-42°C) lasts about one week. Nasal discharge, salivation, hyperemia, hemorrhages of the oral and nasal mucosa are observed 24-48 hrs after onset of fever. After the next few days edema of lips, tongue, face and ears is developed. At the end of febrile period, when mouth lesions begin to heal, coronitis may occur. The pathogenesis of BTV infection is similar in all species of ruminants. After cutaneous infection of BTV, by inoculation or through the bite of a BTV-infected Culicoides vector, the virus travels along the blood vessels to the regional lymph node, the place of the first replication of BTV. The virus is then disseminated to a variety of tissues throughout the body (particularly lungs and spleen) where replication occurs principally in mononuclear phagocytic and endothelial cells, lymphocytes and other cell types. BTV replicates in endothelial cells, causing cell injury and necrosis and leading to vascular thrombosis, tissue infarction, and, consequently, to a dysfunction of organs.
9

Szczepionki przeciwko pryszczycy i perspektywy ich rozwoju

63%
Paprocka G., Kesy A.
Biotechnologia
|
1993
|
nr 2
97-104
10

Postęp w dziedzinie badań nad pryszczycą

63%
Paprocka G., Kesy A.
Postępy Mikrobiologii
|
1986
|
tom 25
|
nr 1-2
11

Pryszczyca - nosicielstwo wirusa u przezuwaczy

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 02
115-118
12

Banki szczepionki przeciwko pryszczycy w Europie - stan obecny i perspektywy

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 04
394-398
The article reviews the history, present status and the future of foot and mouth disease (FMD) vaccine banks in Europe. FMD vaccine banks are those holding reserves of fully formulated and tested vaccines ready for use, and those holding reserves of tested inactivated and concentrated antigen that can be formulated into vaccine. There are essentially four types of FMD vaccine banks in Europe: the International Vaccine Bank (IVB), the European Union Vaccine Bank (EUVB), the All Russian Research Institute for Animal Health (ARRIAH) Vaccine Bank and twelve National Government Vaccine Banks (NGVB). The establishing of these banks, their current location, consistent FMD virus serotypes and strains, as well as their utilization have been summarized. The most recent recommendations on FMD virus strains to be included in FMD vaccines banks, based on current epidemiological intelligence, have been presented. The turnover and replacement of banked antigen is necessary due to changes of FMD epidemiological situation. In an era of increasing global risk of the spread of FMD, vaccine banks will have a strategic role in the control and eradication of FMD.
13

Zastosowanie sondy molekularnej do wykrywania wirusa pryszczycy

63%
Paprocka G., Kesy A.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 03
227-229
14

Pryszczyca - wystepowanie i zwalczanie choroby

63%
Paprocka G., Kesy A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 03
251-253
The review presents a short description of foot-and-mouth disease (FMD) - a highly contagious disease and important from the economic point of view. It describes the clinical symptoms of FMD and the present global FMD situation as well as control measures. In addition it describes the latest fast diagnostic assays.
15

Choroba niebieskiego jezyka - zagrozenie dla Polski

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 11
1271-1274
The aim of this paper was to evaluate the present bluetongue (BT) epidemiological situation in Europe and to present the results of BT monitoring studies carried out in the Department of Foot-and-Mouth Disease of the National Veterinary Research Institute. In 2007 BT spread to the regions of Europe where it has never been observed before. The first cases of BT were reported in the United Kingdom, Denmark, Switzerland and Czech Republic. In total, 31 598 BT outbreaks caused by BTV-8 were found in Europe. Most of the outbreaks were observed between the 34th and 41st week of 2007. Moreover, in some south European countries BT outbreaks caused by serotypes 1 and 4 (Portugal and Spain) and 1, 2, 4, 9 and 16 (Italy and Malta) were found. The BT monitoring studies initiated in our laboratory in September 2006 were continued in 2007. By January 2008 about 9850 serum and blood samples collected from animals imported into Poland from European countries affected by BTV were tested. We found 15 seroreagents, all among the cattle imported from Germany. On the 5th of December, for the first time, the viral RNA was detected by real-time RT-PCR in blood samples of seroreagent from Germany. The presence of viral RNA was detected again in three samples of EDTA blood collected from German cows imported into Poland in November 2007. These animals can be a potential source of BTV in our country after culicoides midges start their seasonal activity in the environment.
16

Serologiczne roznicowanie zwierzat szczepionych od zakazonych wirusem pryszczycy

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 01
20-23
Foot-and-mouth disease (FMD) is a severe and highly infectious viral disease of cloven-hoofed animals. Differentiating FMDV-infected animals from those that have merely been vaccinated is important for international and local trade of live animals, FMD control programs and in particular for eradication campaigns where emergency vaccinations have been applied. Several diagnostic tests have been developed to distinguish between these animals and are all based on detecting antibodies for non-structural proteins (NSPs) of FMDV. These assays have been described using either panels of proteins or individual proteins 3D, 2C, 3AB1 or 3ABC. The response to 3ABC and its cleavage products (mainly 3AB, 3A and 3B) seem to be the most reliable indices of infection. There are four commercially available tests for antibodies to FMDV NSPs: CHEKIT FMD-3ABC (Bommeli AG, Switzerland), Ceditest FMDV-NS (Cedi-Dignostics, B.V. the Netherlands), SVANOIR FMDV 3ABC-Ab ELISA (Svanova Biotech AB, Sweden) as well as UBI FMDV NSP ELISA (United Biomedical, Inc., USA). These kits have been validated to some extent but need to be harmonized and standardized against a set of standards which should cover different epidemiological situations.
17

Zastosowania hodowli komorek do namnazania wirusa pryszczycy

63%
Paprocka G., Kesy A.
Biotechnologia
|
1992
|
nr 3
15-20
18

Ryzyko rozprzestrzeniania wirusa pryszczycy przez zwierzęta dzikie

63%
Paprocka G., Kesy A.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 06
Foot-and-mouth-disease (FMD) is a highly contagious viral disease that primarily affects cloven-hooved livestock and wildlife. The virus can infect members of the Artiodactyla order as well as a few species in other orders. Each species varies in its susceptibility to infection and clinical disease, as well as in its ability to transmit the virus to other animals. Livestock susceptible to FMD are mainly cattle, pigs, sheep and goats. FMD virus can also infect species of wild animals, including African buffalo, bison, elk, wild boar, warthogs, kudu, impala, gazelles and several species of deer. Susceptible non cloven-hooved species include hedgehogs, capybaras, guinea pigs, rats and mice. With these exceptions, wildlife hosts do not seem to be able to maintain FMD viruses, and are usually infected by contact with domesticated livestock. The pathogenicity of FMD among wildlife ranges from asymptomatic to fatal. The signs of FMD in wildlife are generally similar to those in domestic animals; FMD vesicles develop at multiple sites, usually on the feet and in the mouth. In enzootic regions, eradication of FMD is complicated by the presence of infected wild animals which may serve as a reservoir of reintroduction to domestic herds. The most important data regarding FMD in wildlife are presented in this article.
19

Walidacja metody ELISA [SPCE] do wykrywania przeciwcial przeciwko wirusowi pryszczycy

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 04
455-458
The aim of this study was to examine the essential validation parameters of a solid-phase competition ELISA (SPCE) assay for the serological detection of foot-and-mouth disease virus (FMDV) antibody serotypes A, O and Asia 1. The sensitivity of the SPCE was calculated from the results of testing positive sera from convalescent and vaccinated cattle. The highest sensitivity of assay (>99% for all FMDV types) was found at PI 41-50 but implementation of a cut-off level above PI 50 resulted in a decrease of ELISA sensitivity. The specificity of SPCE was examined by testing 760 samples of FMDV-negative field sera collected from healthy, neither infected nor previously vaccinated cattle. In contrast to the sensitivity, an increase in ELISA specificity was observed in tested successive PI ranges and 100% was achieved at PI 61-70. The accuracy of assay was determined by the estimation of repeatability and reproducibility of results. These were performed using a panel of reference sera distributed by the World Reference Laboratory for Foot-and-Mouth Disease (WRL FMD) for the purpose of Phase XVIII collaborative studies. Repeatability and reproducibility were expressed as a coefficient of variation (CV) by analyzing the results of tested reference sera. In case of reproducibility, the CVs for 9 of 10 tested sera ranged from 6.6 to 9.8%. The results of reproducibility were more variable - CVs for all reference sera were generally higher and for two samples (weak positive for FMDV serotype A) they exceeded the acceptable maximum limit.
20

Wystepowanie przeciwcial przeciwko wirusowi choroby niebieskiego jezyka w surowicy zwierzat sprowadzonych do Polski

63%
Niedbalski W., Kesy A.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 01
76-79
The aim of this study was to determine of seroprevalence of bluetongue (BT) virus specific antibodies in susceptible animals imported into Poland after 15 June 2006 from Germany, the Netherlands, France and Belgium. From 1 September to 31 December 2006, 5757 samples of sera supplied from 16 voivodeships were tested by c-ELISA, and positive results were confirmed using agar gel immunodiffusion (AGID). Out of all tested sera, only 3 samples from Zachodniopomorskie Voivodeship were found to be positive for antibodies to BTV (0.05% of the total number of sera samples). The seroreagents were held on a farm in the Swidwin district but their country of origin was the Netherlands. No virus was detected by RT-PCR in EDTA blood samples taken from seroreagents. The results obtained in the National Veterinary Research Institute confirmed by the Community Reference Laboratory for BT in Pirbright (England), where antibodies against BTV-8 were detected in the supplied sera samples.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 4 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.